The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis
Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with ch...
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| Veröffentlicht in: | PloS one 2013-07, Vol.8 (7), p.e67719-e67719 |
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| creator | Gmiterek, Anna Wójtowicz, Halina Mackiewicz, Paweł Radwan-Oczko, Małgorzata Kantorowicz, Małgorzata Chomyszyn-Gajewska, Maria Frąszczak, Magdalena Bielecki, Marcin Olczak, Mariusz Olczak, Teresa |
| description | Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis. |
| doi_str_mv | 10.1371/journal.pone.0067719 |
| format | Article |
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The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067719</identifier><identifier>PMID: 23844074</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Amino acid sequence ; Anaerobic bacteria ; Antibiotics ; Bacteria ; Bacterial infections ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteroidaceae Infections - microbiology ; Bacteroides ; Bacteroides gingivalis ; Base Sequence ; Biochemistry ; Biological markers ; Biology ; Biomarkers - analysis ; Biotechnology ; Case-Control Studies ; Chronic Periodontitis - microbiology ; Deoxyribonucleic acid ; Disease ; DNA ; Etiology ; Female ; Genes ; Genetic aspects ; Genomes ; Genomics ; Green chemistry ; Green development ; Gum disease ; Health risks ; Heme ; Heme - metabolism ; Hemeproteins - metabolism ; HmuY protein ; Humans ; Hybridization ; Infections ; Laboratories ; Male ; Medicine ; Microbiota ; Middle Aged ; Molecular Sequence Data ; Nucleotide sequence ; Pathogens ; Patients ; Periodontitis ; Phylogeny ; Porphyromonas gingivalis ; Porphyromonas gingivalis - classification ; Porphyromonas gingivalis - genetics ; Porphyromonas gingivalis - isolation & purification ; Primers ; RNA polymerase ; Sequence Alignment ; Strains (organisms) ; Teeth</subject><ispartof>PloS one, 2013-07, Vol.8 (7), p.e67719-e67719</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Gmiterek et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Gmiterek et al 2013 Gmiterek et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6032384655f638f81b11ca95aca045359d1083068ed7b3e4f140f7266af722ec3</citedby><cites>FETCH-LOGICAL-c692t-6032384655f638f81b11ca95aca045359d1083068ed7b3e4f140f7266af722ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699645/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699645/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23844074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mokrousov, Igor</contributor><creatorcontrib>Gmiterek, Anna</creatorcontrib><creatorcontrib>Wójtowicz, Halina</creatorcontrib><creatorcontrib>Mackiewicz, Paweł</creatorcontrib><creatorcontrib>Radwan-Oczko, Małgorzata</creatorcontrib><creatorcontrib>Kantorowicz, Małgorzata</creatorcontrib><creatorcontrib>Chomyszyn-Gajewska, Maria</creatorcontrib><creatorcontrib>Frąszczak, Magdalena</creatorcontrib><creatorcontrib>Bielecki, Marcin</creatorcontrib><creatorcontrib>Olczak, Mariusz</creatorcontrib><creatorcontrib>Olczak, Teresa</creatorcontrib><title>The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis.</description><subject>Adult</subject><subject>Aged</subject><subject>Amino acid sequence</subject><subject>Anaerobic bacteria</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteroidaceae Infections - microbiology</subject><subject>Bacteroides</subject><subject>Bacteroides gingivalis</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological markers</subject><subject>Biology</subject><subject>Biomarkers - analysis</subject><subject>Biotechnology</subject><subject>Case-Control Studies</subject><subject>Chronic Periodontitis - microbiology</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>Etiology</subject><subject>Female</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Green chemistry</subject><subject>Green development</subject><subject>Gum disease</subject><subject>Health risks</subject><subject>Heme</subject><subject>Heme - metabolism</subject><subject>Hemeproteins - metabolism</subject><subject>HmuY protein</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medicine</subject><subject>Microbiota</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequence</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Periodontitis</subject><subject>Phylogeny</subject><subject>Porphyromonas gingivalis</subject><subject>Porphyromonas gingivalis - classification</subject><subject>Porphyromonas gingivalis - genetics</subject><subject>Porphyromonas gingivalis - isolation & purification</subject><subject>Primers</subject><subject>RNA polymerase</subject><subject>Sequence Alignment</subject><subject>Strains (organisms)</subject><subject>Teeth</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7jr6D0QLgujFjPlq0twIy-LHwOKKroJXIZOetlnbZEzaxf33ZpzuMpW9kELaJs95cz7eLHuK0QpTgd9c-jE43a223sEKIS4ElveyYywpWXKC6P2D76PsUYyXCBW05PxhdkRoyRgS7Dj7dNFCPjr7a4S87ccfeQMO8gjp3xnIdcx1HrdgbG1N3uvwE0Lu6_yzD9v2Ovjeu4Q01jX2Snc2Ps4e1LqL8GR6L7Jv799dnH5cnp1_WJ-enC0Nl2RYckR3KfCiqDkt6xJvMDZaFtpoxApayAqjkiJeQiU2FFiNGaoF4VynlYChi-z5Xnfb-aimVkSFpWBFSUtBE7HeE5XXl2obbEr-Wnlt1d8NHxqlw2BNB6pEiBabSgAmJasMk7UUqNxUpCgQIYVIWm-n28ZND5UBNwTdzUTnJ862qvFXinIpeSpokb2aBIJPnY2D6m000HXagR9T3lRKSdIIZUJf_IPeXd1ENToVYF3t071mJ6pOmEjDZUTutFZ3UOmpoLcm-aa2aX8W8HoWkJgBfg-NHmNU669f_p89_z5nXx6wLehuaKPvxsF6F-cg24Mm-BgD1LdNxkjtbH_TDbWzvZpsn8KeHQ7oNujG5_QPx-H67A</recordid><startdate>20130702</startdate><enddate>20130702</enddate><creator>Gmiterek, Anna</creator><creator>Wójtowicz, Halina</creator><creator>Mackiewicz, Paweł</creator><creator>Radwan-Oczko, Małgorzata</creator><creator>Kantorowicz, Małgorzata</creator><creator>Chomyszyn-Gajewska, Maria</creator><creator>Frąszczak, Magdalena</creator><creator>Bielecki, Marcin</creator><creator>Olczak, Mariusz</creator><creator>Olczak, Teresa</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130702</creationdate><title>The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis</title><author>Gmiterek, Anna ; Wójtowicz, Halina ; Mackiewicz, Paweł ; Radwan-Oczko, Małgorzata ; Kantorowicz, Małgorzata ; Chomyszyn-Gajewska, Maria ; Frąszczak, Magdalena ; Bielecki, Marcin ; Olczak, Mariusz ; Olczak, Teresa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6032384655f638f81b11ca95aca045359d1083068ed7b3e4f140f7266af722ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amino acid sequence</topic><topic>Anaerobic bacteria</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteroidaceae Infections - microbiology</topic><topic>Bacteroides</topic><topic>Bacteroides gingivalis</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological markers</topic><topic>Biology</topic><topic>Biomarkers - analysis</topic><topic>Biotechnology</topic><topic>Case-Control Studies</topic><topic>Chronic Periodontitis - microbiology</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>Etiology</topic><topic>Female</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Green chemistry</topic><topic>Green development</topic><topic>Gum disease</topic><topic>Health risks</topic><topic>Heme</topic><topic>Heme - metabolism</topic><topic>Hemeproteins - metabolism</topic><topic>HmuY protein</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medicine</topic><topic>Microbiota</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequence</topic><topic>Pathogens</topic><topic>Patients</topic><topic>Periodontitis</topic><topic>Phylogeny</topic><topic>Porphyromonas gingivalis</topic><topic>Porphyromonas gingivalis - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gmiterek, Anna</au><au>Wójtowicz, Halina</au><au>Mackiewicz, Paweł</au><au>Radwan-Oczko, Małgorzata</au><au>Kantorowicz, Małgorzata</au><au>Chomyszyn-Gajewska, Maria</au><au>Frąszczak, Magdalena</au><au>Bielecki, Marcin</au><au>Olczak, Mariusz</au><au>Olczak, Teresa</au><au>Mokrousov, Igor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-07-02</date><risdate>2013</risdate><volume>8</volume><issue>7</issue><spage>e67719</spage><epage>e67719</epage><pages>e67719-e67719</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23844074</pmid><doi>10.1371/journal.pone.0067719</doi><tpages>e67719</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-07, Vol.8 (7), p.e67719-e67719 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1974583873 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Amino acid sequence Anaerobic bacteria Antibiotics Bacteria Bacterial infections Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteroidaceae Infections - microbiology Bacteroides Bacteroides gingivalis Base Sequence Biochemistry Biological markers Biology Biomarkers - analysis Biotechnology Case-Control Studies Chronic Periodontitis - microbiology Deoxyribonucleic acid Disease DNA Etiology Female Genes Genetic aspects Genomes Genomics Green chemistry Green development Gum disease Health risks Heme Heme - metabolism Hemeproteins - metabolism HmuY protein Humans Hybridization Infections Laboratories Male Medicine Microbiota Middle Aged Molecular Sequence Data Nucleotide sequence Pathogens Patients Periodontitis Phylogeny Porphyromonas gingivalis Porphyromonas gingivalis - classification Porphyromonas gingivalis - genetics Porphyromonas gingivalis - isolation & purification Primers RNA polymerase Sequence Alignment Strains (organisms) Teeth |
| title | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis |
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