Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry

The widely used interaction of the homotetramer streptavidin with the small molecule biotin has been intensively studied by force spectroscopy and has become a model system for receptor ligand interaction. However, streptavidin's tetravalency results in diverse force propagation pathways throug...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2017-12, Vol.12 (12), p.e0188722-e0188722
Hauptverfasser:	Sedlak, Steffen M, Bauer, Magnus S, Kluger, Carleen, Schendel, Leonard C, Milles, Lukas F, Pippig, Diana A, Gaub, Hermann E
Format:	Artikel
Sprache:	eng
Schlagworte:	Atomic beam spectroscopy Atomic force microscopy Binding sites Biology and Life Sciences Biotin Cysteine Dosage and administration Experiments Health aspects Immobilization Interfaces Ligands Measurement Mechanical properties Medicine and Health Sciences Microscopy Nanoparticles Physical Sciences Polyethylene glycol Potential barriers Research and Analysis Methods Spectroscopy Spectrum analysis Stoichiometry Streptavidin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!