BRITER: a BMP responsive osteoblast reporter cell line
BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore impo...
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| Veröffentlicht in: | PloS one 2012-05, Vol.7 (5), p.e37134-e37134 |
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| creator | Yadav, Prem Swaroop Prashar, Paritosh Bandyopadhyay, Amitabha |
| description | BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens.
We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins.
As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance. |
| doi_str_mv | 10.1371/journal.pone.0037134 |
| format | Article |
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We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins.
As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0037134</identifier><identifier>PMID: 22611465</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biocompatibility ; Bioengineering ; Biology ; Bone morphogenetic proteins ; Bone Morphogenetic Proteins - metabolism ; Bone Morphogenetic Proteins - pharmacology ; Cell Line ; Cloning ; Colorectal cancer ; Cooperation ; Fractures ; Gene expression ; Genes, Reporter ; Genetic screening ; Genetic testing ; Genomes ; Growth factors ; Homeostasis ; Humans ; Influenza ; Journalists ; Kinases ; Laboratories ; Luciferase ; Mice ; Mice, Knockout ; Motility ; Mutation ; Osteoblasts - metabolism ; Proteins ; Screening ; Signal Transduction ; Signaling ; Tamoxifen</subject><ispartof>PloS one, 2012-05, Vol.7 (5), p.e37134-e37134</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Yadav et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Yadav et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c320c74ad17d71a8f30c18ab633641115921d592166219622ccf51a692fc1bd53</citedby><cites>FETCH-LOGICAL-c692t-c320c74ad17d71a8f30c18ab633641115921d592166219622ccf51a692fc1bd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354957/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354957/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22611465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Cooney, Austin John</contributor><creatorcontrib>Yadav, Prem Swaroop</creatorcontrib><creatorcontrib>Prashar, Paritosh</creatorcontrib><creatorcontrib>Bandyopadhyay, Amitabha</creatorcontrib><title>BRITER: a BMP responsive osteoblast reporter cell line</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens.
We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins.
As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Bioengineering</subject><subject>Biology</subject><subject>Bone morphogenetic proteins</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Bone Morphogenetic Proteins - pharmacology</subject><subject>Cell Line</subject><subject>Cloning</subject><subject>Colorectal cancer</subject><subject>Cooperation</subject><subject>Fractures</subject><subject>Gene expression</subject><subject>Genes, Reporter</subject><subject>Genetic screening</subject><subject>Genetic testing</subject><subject>Genomes</subject><subject>Growth factors</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Influenza</subject><subject>Journalists</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Luciferase</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Motility</subject><subject>Mutation</subject><subject>Osteoblasts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yadav, Prem Swaroop</au><au>Prashar, Paritosh</au><au>Bandyopadhyay, Amitabha</au><au>Cooney, Austin John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BRITER: a BMP responsive osteoblast reporter cell line</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-05-14</date><risdate>2012</risdate><volume>7</volume><issue>5</issue><spage>e37134</spage><epage>e37134</epage><pages>e37134-e37134</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens.
We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins.
As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22611465</pmid><doi>10.1371/journal.pone.0037134</doi><tpages>e37134</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biocompatibility Bioengineering Biology Bone morphogenetic proteins Bone Morphogenetic Proteins - metabolism Bone Morphogenetic Proteins - pharmacology Cell Line Cloning Colorectal cancer Cooperation Fractures Gene expression Genes, Reporter Genetic screening Genetic testing Genomes Growth factors Homeostasis Humans Influenza Journalists Kinases Laboratories Luciferase Mice Mice, Knockout Motility Mutation Osteoblasts - metabolism Proteins Screening Signal Transduction Signaling Tamoxifen |
| title | BRITER: a BMP responsive osteoblast reporter cell line |
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