Yeast casein kinase 2 governs morphology, biofilm formation, cell wall integrity, and host cell damage of Candida albicans
The regulatory networks governing morphogenesis of a pleomorphic fungus, Candida albicans are extremely complex and remain to be completely elucidated. This study investigated the function of C. albicans yeast casein kinase 2 (CaYck2p). The yck2Δ/yck2Δ strain displayed constitutive pseudohyphae in b...
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| description | The regulatory networks governing morphogenesis of a pleomorphic fungus, Candida albicans are extremely complex and remain to be completely elucidated. This study investigated the function of C. albicans yeast casein kinase 2 (CaYck2p). The yck2Δ/yck2Δ strain displayed constitutive pseudohyphae in both yeast and hyphal growth conditions, and formed enhanced biofilm under non-biofilm inducing condition. This finding was further supported by gene expression analysis of the yck2Δ/yck2Δ strain which showed significant upregulation of UME6, a key transcriptional regulator of hyphal transition and biofilm formation, and cell wall protein genes ALS3, HWP1, and SUN41, all of which are associated with morphogenesis and biofilm architecture. The yck2Δ/yck2Δ strain was hypersensitive to cell wall damaging agents and had increased compensatory chitin deposition in the cell wall accompanied by an upregulation of the expression of the chitin synthase genes, CHS2, CHS3, and CHS8. Absence of CaYck2p also affected fungal-host interaction; the yck2Δ/yck2Δ strain had significantly reduced ability to damage host cells. However, the yck2Δ/yck2Δ strain had wild-type susceptibility to cyclosporine and FK506, suggesting that CaYck2p functions independently from the Ca+/calcineurin pathway. Thus, in C. albicans, Yck2p is a multifunctional kinase that governs morphogenesis, biofilm formation, cell wall integrity, and host cell interactions. |
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This study investigated the function of C. albicans yeast casein kinase 2 (CaYck2p). The yck2Δ/yck2Δ strain displayed constitutive pseudohyphae in both yeast and hyphal growth conditions, and formed enhanced biofilm under non-biofilm inducing condition. This finding was further supported by gene expression analysis of the yck2Δ/yck2Δ strain which showed significant upregulation of UME6, a key transcriptional regulator of hyphal transition and biofilm formation, and cell wall protein genes ALS3, HWP1, and SUN41, all of which are associated with morphogenesis and biofilm architecture. The yck2Δ/yck2Δ strain was hypersensitive to cell wall damaging agents and had increased compensatory chitin deposition in the cell wall accompanied by an upregulation of the expression of the chitin synthase genes, CHS2, CHS3, and CHS8. Absence of CaYck2p also affected fungal-host interaction; the yck2Δ/yck2Δ strain had significantly reduced ability to damage host cells. However, the yck2Δ/yck2Δ strain had wild-type susceptibility to cyclosporine and FK506, suggesting that CaYck2p functions independently from the Ca+/calcineurin pathway. Thus, in C. albicans, Yck2p is a multifunctional kinase that governs morphogenesis, biofilm formation, cell wall integrity, and host cell interactions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0187721</identifier><identifier>PMID: 29107946</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Baking yeast ; Biofilms ; Biofilms - growth & development ; Biology and Life Sciences ; Biosynthesis ; Calcineurin ; Candida ; Candida albicans ; Candida albicans - enzymology ; Candida albicans - growth & development ; Casein ; Casein kinase II ; Casein Kinase II - metabolism ; Cell cycle ; Cell interactions ; Cell Wall ; Cell walls ; Chitin ; Chitin - biosynthesis ; Chitin synthase ; Cyclosporins ; Cytology ; Damage ; Gene expression ; Genes ; Genomes ; Growth conditions ; Infectious diseases ; Integrity ; Kinases ; Medicine and Health Sciences ; Morphogenesis ; Morphology ; Physical Sciences ; Proteins ; Pseudohyphae ; Real-Time Polymerase Chain Reaction ; Regulation ; Research and Analysis Methods ; Saccharomyces cerevisiae ; Tacrolimus ; Transcription ; Yeast</subject><ispartof>PloS one, 2017-11, Vol.12 (11), p.e0187721-e0187721</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Jung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Jung et al 2017 Jung et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-66fdad2c3a5bd7dabf2cccb2fd072d0cce38a5af5f264615090e6e9eb755232b3</citedby><cites>FETCH-LOGICAL-c692t-66fdad2c3a5bd7dabf2cccb2fd072d0cce38a5af5f264615090e6e9eb755232b3</cites><orcidid>0000-0002-8065-1625</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673188/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673188/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29107946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sturtevant, Joy</contributor><creatorcontrib>Jung, Sook-In</creatorcontrib><creatorcontrib>Rodriguez, Natalie</creatorcontrib><creatorcontrib>Irrizary, Jihyun</creatorcontrib><creatorcontrib>Liboro, Karl</creatorcontrib><creatorcontrib>Bogarin, Thania</creatorcontrib><creatorcontrib>Macias, Marlene</creatorcontrib><creatorcontrib>Eivers, Edward</creatorcontrib><creatorcontrib>Porter, Edith</creatorcontrib><creatorcontrib>Filler, Scott G</creatorcontrib><creatorcontrib>Park, Hyunsook</creatorcontrib><title>Yeast casein kinase 2 governs morphology, biofilm formation, cell wall integrity, and host cell damage of Candida albicans</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The regulatory networks governing morphogenesis of a pleomorphic fungus, Candida albicans are extremely complex and remain to be completely elucidated. This study investigated the function of C. albicans yeast casein kinase 2 (CaYck2p). The yck2Δ/yck2Δ strain displayed constitutive pseudohyphae in both yeast and hyphal growth conditions, and formed enhanced biofilm under non-biofilm inducing condition. This finding was further supported by gene expression analysis of the yck2Δ/yck2Δ strain which showed significant upregulation of UME6, a key transcriptional regulator of hyphal transition and biofilm formation, and cell wall protein genes ALS3, HWP1, and SUN41, all of which are associated with morphogenesis and biofilm architecture. The yck2Δ/yck2Δ strain was hypersensitive to cell wall damaging agents and had increased compensatory chitin deposition in the cell wall accompanied by an upregulation of the expression of the chitin synthase genes, CHS2, CHS3, and CHS8. Absence of CaYck2p also affected fungal-host interaction; the yck2Δ/yck2Δ strain had significantly reduced ability to damage host cells. However, the yck2Δ/yck2Δ strain had wild-type susceptibility to cyclosporine and FK506, suggesting that CaYck2p functions independently from the Ca+/calcineurin pathway. Thus, in C. albicans, Yck2p is a multifunctional kinase that governs morphogenesis, biofilm formation, cell wall integrity, and host cell interactions.</description><subject>Baking yeast</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Calcineurin</subject><subject>Candida</subject><subject>Candida albicans</subject><subject>Candida albicans - enzymology</subject><subject>Candida albicans - growth & development</subject><subject>Casein</subject><subject>Casein kinase II</subject><subject>Casein Kinase II - metabolism</subject><subject>Cell cycle</subject><subject>Cell interactions</subject><subject>Cell Wall</subject><subject>Cell walls</subject><subject>Chitin</subject><subject>Chitin - biosynthesis</subject><subject>Chitin synthase</subject><subject>Cyclosporins</subject><subject>Cytology</subject><subject>Damage</subject><subject>Gene 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casein kinase 2 governs morphology, biofilm formation, cell wall integrity, and host cell damage of Candida albicans</title><author>Jung, Sook-In ; Rodriguez, Natalie ; Irrizary, Jihyun ; Liboro, Karl ; Bogarin, Thania ; Macias, Marlene ; Eivers, Edward ; Porter, Edith ; Filler, Scott G ; Park, Hyunsook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-66fdad2c3a5bd7dabf2cccb2fd072d0cce38a5af5f264615090e6e9eb755232b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Baking yeast</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Calcineurin</topic><topic>Candida</topic><topic>Candida albicans</topic><topic>Candida albicans - enzymology</topic><topic>Candida albicans - growth & development</topic><topic>Casein</topic><topic>Casein kinase II</topic><topic>Casein Kinase II - 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One</addtitle><date>2017-11-06</date><risdate>2017</risdate><volume>12</volume><issue>11</issue><spage>e0187721</spage><epage>e0187721</epage><pages>e0187721-e0187721</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The regulatory networks governing morphogenesis of a pleomorphic fungus, Candida albicans are extremely complex and remain to be completely elucidated. This study investigated the function of C. albicans yeast casein kinase 2 (CaYck2p). The yck2Δ/yck2Δ strain displayed constitutive pseudohyphae in both yeast and hyphal growth conditions, and formed enhanced biofilm under non-biofilm inducing condition. This finding was further supported by gene expression analysis of the yck2Δ/yck2Δ strain which showed significant upregulation of UME6, a key transcriptional regulator of hyphal transition and biofilm formation, and cell wall protein genes ALS3, HWP1, and SUN41, all of which are associated with morphogenesis and biofilm architecture. The yck2Δ/yck2Δ strain was hypersensitive to cell wall damaging agents and had increased compensatory chitin deposition in the cell wall accompanied by an upregulation of the expression of the chitin synthase genes, CHS2, CHS3, and CHS8. Absence of CaYck2p also affected fungal-host interaction; the yck2Δ/yck2Δ strain had significantly reduced ability to damage host cells. However, the yck2Δ/yck2Δ strain had wild-type susceptibility to cyclosporine and FK506, suggesting that CaYck2p functions independently from the Ca+/calcineurin pathway. Thus, in C. albicans, Yck2p is a multifunctional kinase that governs morphogenesis, biofilm formation, cell wall integrity, and host cell interactions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29107946</pmid><doi>10.1371/journal.pone.0187721</doi><tpages>e0187721</tpages><orcidid>https://orcid.org/0000-0002-8065-1625</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Baking yeast Biofilms Biofilms - growth & development Biology and Life Sciences Biosynthesis Calcineurin Candida Candida albicans Candida albicans - enzymology Candida albicans - growth & development Casein Casein kinase II Casein Kinase II - metabolism Cell cycle Cell interactions Cell Wall Cell walls Chitin Chitin - biosynthesis Chitin synthase Cyclosporins Cytology Damage Gene expression Genes Genomes Growth conditions Infectious diseases Integrity Kinases Medicine and Health Sciences Morphogenesis Morphology Physical Sciences Proteins Pseudohyphae Real-Time Polymerase Chain Reaction Regulation Research and Analysis Methods Saccharomyces cerevisiae Tacrolimus Transcription Yeast |
| title | Yeast casein kinase 2 governs morphology, biofilm formation, cell wall integrity, and host cell damage of Candida albicans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T14%3A30%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Yeast%20casein%20kinase%202%20governs%20morphology,%20biofilm%20formation,%20cell%20wall%20integrity,%20and%20host%20cell%20damage%20of%20Candida%20albicans&rft.jtitle=PloS%20one&rft.au=Jung,%20Sook-In&rft.date=2017-11-06&rft.volume=12&rft.issue=11&rft.spage=e0187721&rft.epage=e0187721&rft.pages=e0187721-e0187721&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0187721&rft_dat=%3Cgale_plos_%3EA513556613%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1961011413&rft_id=info:pmid/29107946&rft_galeid=A513556613&rft_doaj_id=oai_doaj_org_article_c5fe26620f2a45d5890675d85a2faa0c&rfr_iscdi=true |