Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study
Previous reports have suggested the significant association of miRNAs aberrant expression with tumor initiation, progression and metastasis in cancer, including gastrointestinal (GI) cancers. The current preliminary study aimed to evaluate the relative expression levels of miR-196a2 and three of its...
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| description | Previous reports have suggested the significant association of miRNAs aberrant expression with tumor initiation, progression and metastasis in cancer, including gastrointestinal (GI) cancers. The current preliminary study aimed to evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients. Quantitative real-time PCR for miR-196a2 and its selected mRNA targets, as well as immunohistochemical assay for annexin A1 protein expression were detected in 58 tissues with different GI cancer samples. In addition, correlation with the clinicopathological features and in silico network analysis of the selected molecular markers were analyzed. Stratified analyses by cancer site revealed elevated levels of miR-196a2 and low expression of the selected target genes. Annexin protein expression was positively correlated with its gene expression profile. In colorectal cancer, miR-196a over-expression was negatively correlated with annexin A1 protein expression (r = -0.738, p < 0.001), and both were indicators of unfavorable prognosis in terms of poor differentiation, larger tumor size, and advanced clinical stage. Taken together, aberrant expression of miR-196a2 and the selected apoptosis-related biomarkers might be involved in GI cancer development and progression and could have potential diagnostic and prognostic roles in these types of cancer; particularly colorectal cancer, provided the results experimentally validated and confirmed in larger multi-center studies. |
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The current preliminary study aimed to evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients. Quantitative real-time PCR for miR-196a2 and its selected mRNA targets, as well as immunohistochemical assay for annexin A1 protein expression were detected in 58 tissues with different GI cancer samples. In addition, correlation with the clinicopathological features and in silico network analysis of the selected molecular markers were analyzed. Stratified analyses by cancer site revealed elevated levels of miR-196a2 and low expression of the selected target genes. Annexin protein expression was positively correlated with its gene expression profile. In colorectal cancer, miR-196a over-expression was negatively correlated with annexin A1 protein expression (r = -0.738, p < 0.001), and both were indicators of unfavorable prognosis in terms of poor differentiation, larger tumor size, and advanced clinical stage. Taken together, aberrant expression of miR-196a2 and the selected apoptosis-related biomarkers might be involved in GI cancer development and progression and could have potential diagnostic and prognostic roles in these types of cancer; particularly colorectal cancer, provided the results experimentally validated and confirmed in larger multi-center studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0187310</identifier><identifier>PMID: 29091952</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>A1 protein ; Aberration ; Adult ; Analysis ; Annexin A1 - genetics ; Apoptosis ; Apoptosis - genetics ; Apoptosis Regulatory Proteins - genetics ; Biochemistry ; Bioindicators ; Biology and Life Sciences ; Biomarkers ; Cancer ; Care and treatment ; Cell cycle ; Cell growth ; Chemotherapy ; Colorectal cancer ; Colorectal carcinoma ; Diagnosis ; Diagnostic systems ; Esophageal cancer ; Female ; Gastric cancer ; Gastrointestinal cancer ; Gastrointestinal Neoplasms - genetics ; Gastrointestinal Neoplasms - pathology ; Gene expression ; Gene Expression Profiling ; Genes ; Humans ; Kinases ; Laboratories ; Liver cancer ; Male ; Medical prognosis ; Medicine ; Medicine and Health Sciences ; Metastases ; Metastasis ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Network analysis ; Ontology ; Overexpression ; Pathology ; Patients ; Pilot Projects ; Proteins ; Ribonucleic acid ; RNA ; RNA-Binding Proteins - genetics ; Stomach cancer ; Studies ; Tissues ; Tumors</subject><ispartof>PloS one, 2017-11, Vol.12 (11), p.e0187310-e0187310</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Fawzy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The current preliminary study aimed to evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients. Quantitative real-time PCR for miR-196a2 and its selected mRNA targets, as well as immunohistochemical assay for annexin A1 protein expression were detected in 58 tissues with different GI cancer samples. In addition, correlation with the clinicopathological features and in silico network analysis of the selected molecular markers were analyzed. Stratified analyses by cancer site revealed elevated levels of miR-196a2 and low expression of the selected target genes. Annexin protein expression was positively correlated with its gene expression profile. 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The current preliminary study aimed to evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients. Quantitative real-time PCR for miR-196a2 and its selected mRNA targets, as well as immunohistochemical assay for annexin A1 protein expression were detected in 58 tissues with different GI cancer samples. In addition, correlation with the clinicopathological features and in silico network analysis of the selected molecular markers were analyzed. Stratified analyses by cancer site revealed elevated levels of miR-196a2 and low expression of the selected target genes. Annexin protein expression was positively correlated with its gene expression profile. In colorectal cancer, miR-196a over-expression was negatively correlated with annexin A1 protein expression (r = -0.738, p < 0.001), and both were indicators of unfavorable prognosis in terms of poor differentiation, larger tumor size, and advanced clinical stage. Taken together, aberrant expression of miR-196a2 and the selected apoptosis-related biomarkers might be involved in GI cancer development and progression and could have potential diagnostic and prognostic roles in these types of cancer; particularly colorectal cancer, provided the results experimentally validated and confirmed in larger multi-center studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29091952</pmid><doi>10.1371/journal.pone.0187310</doi><tpages>e0187310</tpages><orcidid>https://orcid.org/0000-0003-1252-8403</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-11, Vol.12 (11), p.e0187310-e0187310 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1958607976 |
| source | Open Access: PubMed Central; PLoS; MEDLINE; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | A1 protein Aberration Adult Analysis Annexin A1 - genetics Apoptosis Apoptosis - genetics Apoptosis Regulatory Proteins - genetics Biochemistry Bioindicators Biology and Life Sciences Biomarkers Cancer Care and treatment Cell cycle Cell growth Chemotherapy Colorectal cancer Colorectal carcinoma Diagnosis Diagnostic systems Esophageal cancer Female Gastric cancer Gastrointestinal cancer Gastrointestinal Neoplasms - genetics Gastrointestinal Neoplasms - pathology Gene expression Gene Expression Profiling Genes Humans Kinases Laboratories Liver cancer Male Medical prognosis Medicine Medicine and Health Sciences Metastases Metastasis MicroRNA MicroRNAs MicroRNAs - genetics miRNA Network analysis Ontology Overexpression Pathology Patients Pilot Projects Proteins Ribonucleic acid RNA RNA-Binding Proteins - genetics Stomach cancer Studies Tissues Tumors |
| title | Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T19%3A46%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20miRNA-196a2%20and%20apoptosis-related%20target%20genes:%20ANXA1,%20DFFA%20and%20PDCD4%20expression%20in%20gastrointestinal%20cancer%20patients:%20A%20pilot%20study&rft.jtitle=PloS%20one&rft.au=Fawzy,%20Manal%20S&rft.date=2017-11-01&rft.volume=12&rft.issue=11&rft.spage=e0187310&rft.epage=e0187310&rft.pages=e0187310-e0187310&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0187310&rft_dat=%3Cgale_plos_%3EA512699661%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1958607976&rft_id=info:pmid/29091952&rft_galeid=A512699661&rft_doaj_id=oai_doaj_org_article_825ec6cd1de14efe872dc240d303a312&rfr_iscdi=true |