Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs
Vitamin D is a secosteroid hormone with many varied functions including regulation of blood calcium levels, cell proliferation, immunity, and reproduction in mammals. Vitamin D is activated by 25-hydroxylase (CYP2R1) and 1-alpha-hydroxylase (CYP27B1) and is degraded by 24-hydroxylase (CYP24A1). Vita...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-10, Vol.12 (10), p.e0187221-e0187221 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0187221 |
|---|---|
| container_issue | 10 |
| container_start_page | e0187221 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Jang, Hwanhee Choi, Yohan Yoo, Inkyu Han, Jisoo Hong, Jin Su Kim, Yoo Yong Ka, Hakhyun |
| description | Vitamin D is a secosteroid hormone with many varied functions including regulation of blood calcium levels, cell proliferation, immunity, and reproduction in mammals. Vitamin D is activated by 25-hydroxylase (CYP2R1) and 1-alpha-hydroxylase (CYP27B1) and is degraded by 24-hydroxylase (CYP24A1). Vitamin D is transported by vitamin D-binding protein (group-specific component, GC) through the bloodstream and regulates cellular actions by binding to vitamin D receptor (VDR). In this study, we determined the expression and regulation of vitamin D-related molecules and the role of vitamin D at the maternal-conceptus interface in pigs. Vitamin D-metabolizing enzymes CYP2R1, CYP27B1, and CYP24A1, vitamin D binding protein GC, and vitamin D receptor VDR were expressed in the endometrium in a pregnancy stage-specific manner as well as in conceptus and chorioallantoic tissues during pregnancy. VDR protein was localized to endometrial and trophoblastic cells. Concentrations of calcitriol, the active form of vitamin D, in the endometrial tissues were higher during early pregnancy than in mid- to late pregnancy, while plasma concentrations of calcitriol were highest during late pregnancy. Furthermore, calcitriol affected the expression of several genes related to conceptus implantation, vitamin D metabolism, calcium ion regulation, PG metabolism, and calcium-binding proteins in endometrial tissue explants. These results show that CYP2R1, CYP27B1, CYP24A1, GC, and VDR were expressed at the maternal-conceptus interface, endometrial calcitriol levels were regulated during pregnancy, and calcitriol modulated the expression of endometrial genes, suggesting that calcitriol may play an important role in the establishment and maintenance of pregnancy by regulating endometrial function in pigs. |
| doi_str_mv | 10.1371/journal.pone.0187221 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1958247977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A512496632</galeid><doaj_id>oai_doaj_org_article_6132b1ec231a4ee584a57ce533737329</doaj_id><sourcerecordid>A512496632</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-d88a1abb4f8a4ba3a2cfc58a07ba1156237026c7ce871f3a9500078d246677553</originalsourceid><addsrcrecordid>eNqNk9tuEzEQhlcIREvhDRBYQkJwkeDDHrlAqkqBSJUqceitNeudTVx57WB7q5bH4UlxmqQkqBfIF7bG3_y_PfZk2XNGp0xU7N2lG70FM106i1PK6opz9iA7ZI3gk5JT8XBnfZA9CeGS0kLUZfk4O-ANrWvesMPs94WOMGhLPk4GjNA6oxVB--tmwEDAdsSjgYgdGZxBNRoM78np9dJjCNpZApHEBZIhIavDTJSzCpdxDETbFOpB4a3KCvJJgbieXG0dE5OsOpeMvQZD5miR4F_xtL3U8_A0e9SDCfhsMx9lPz6dfj_5Mjk7_zw7OT6bqLLhcdLVNTBo27yvIW9BAFe9KmqgVQuMFSUXFeWlqhTWFesFNAWltKo7npdlVRWFOMpernWXxgW5KW-QrClqnldNVSVitiY6B5dy6fUA_kY60PI24Pxcgo9aGZQlE7xlqLhgkCMWdQ5Fsi6EqNLgTdL6sHEb2wE7hTZ6MHui-ztWL-TcXcmiLEUueBJ4sxHw7ueIIcpBB4XGgEU3rs9d5KykLKGv_kHvv92GmkO6gLa9S75qJSqPC8bzJhmvbKf3UGl0OOj0_NjrFN9LeLuXkJiI13EOYwhy9u3r_7PnF_vs6x12gWDiIjgzxvR1wj6Yr0HlXQge-7siMypXrbSthly1kty0Ukp7sftAd0nb3hF_AATrGqs</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1958247977</pqid></control><display><type>article</type><title>Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Jang, Hwanhee ; Choi, Yohan ; Yoo, Inkyu ; Han, Jisoo ; Hong, Jin Su ; Kim, Yoo Yong ; Ka, Hakhyun</creator><creatorcontrib>Jang, Hwanhee ; Choi, Yohan ; Yoo, Inkyu ; Han, Jisoo ; Hong, Jin Su ; Kim, Yoo Yong ; Ka, Hakhyun</creatorcontrib><description>Vitamin D is a secosteroid hormone with many varied functions including regulation of blood calcium levels, cell proliferation, immunity, and reproduction in mammals. Vitamin D is activated by 25-hydroxylase (CYP2R1) and 1-alpha-hydroxylase (CYP27B1) and is degraded by 24-hydroxylase (CYP24A1). Vitamin D is transported by vitamin D-binding protein (group-specific component, GC) through the bloodstream and regulates cellular actions by binding to vitamin D receptor (VDR). In this study, we determined the expression and regulation of vitamin D-related molecules and the role of vitamin D at the maternal-conceptus interface in pigs. Vitamin D-metabolizing enzymes CYP2R1, CYP27B1, and CYP24A1, vitamin D binding protein GC, and vitamin D receptor VDR were expressed in the endometrium in a pregnancy stage-specific manner as well as in conceptus and chorioallantoic tissues during pregnancy. VDR protein was localized to endometrial and trophoblastic cells. Concentrations of calcitriol, the active form of vitamin D, in the endometrial tissues were higher during early pregnancy than in mid- to late pregnancy, while plasma concentrations of calcitriol were highest during late pregnancy. Furthermore, calcitriol affected the expression of several genes related to conceptus implantation, vitamin D metabolism, calcium ion regulation, PG metabolism, and calcium-binding proteins in endometrial tissue explants. These results show that CYP2R1, CYP27B1, CYP24A1, GC, and VDR were expressed at the maternal-conceptus interface, endometrial calcitriol levels were regulated during pregnancy, and calcitriol modulated the expression of endometrial genes, suggesting that calcitriol may play an important role in the establishment and maintenance of pregnancy by regulating endometrial function in pigs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0187221</identifier><identifier>PMID: 29088291</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism ; Agricultural biotechnology ; Animals ; Biology and Life Sciences ; Calcitriol ; Calcium ; Calcium (blood) ; Calcium metabolism ; Cell proliferation ; Cholestanetriol 26-Monooxygenase - metabolism ; Chorioallantoic Membrane - metabolism ; Cytochrome P-450 ; Cytokines ; Endometrium ; Endometrium - metabolism ; Endometrium - physiology ; Enzymes ; Explants ; Female ; Gene expression ; Gene Expression Regulation - physiology ; Genes ; Guanylate cyclase ; Hogs ; Homeostasis ; Hydroxylase ; Immunity ; Implantation ; Medicine and Health Sciences ; Metabolism ; Physical sciences ; Physiological aspects ; Physiology ; Pigs ; Pregnancy ; Protein binding ; Proteins ; Receptors, Calcitriol - metabolism ; Rodents ; Swine ; Tissues ; Vitamin D ; Vitamin D - metabolism ; Vitamin D - physiology ; Vitamin D receptors ; Vitamin D-binding protein ; Vitamin D-Binding Protein - metabolism ; Vitamin D3 24-Hydroxylase - metabolism</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0187221-e0187221</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Jang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Jang et al 2017 Jang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d88a1abb4f8a4ba3a2cfc58a07ba1156237026c7ce871f3a9500078d246677553</citedby><cites>FETCH-LOGICAL-c692t-d88a1abb4f8a4ba3a2cfc58a07ba1156237026c7ce871f3a9500078d246677553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663432/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663432/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29088291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Hwanhee</creatorcontrib><creatorcontrib>Choi, Yohan</creatorcontrib><creatorcontrib>Yoo, Inkyu</creatorcontrib><creatorcontrib>Han, Jisoo</creatorcontrib><creatorcontrib>Hong, Jin Su</creatorcontrib><creatorcontrib>Kim, Yoo Yong</creatorcontrib><creatorcontrib>Ka, Hakhyun</creatorcontrib><title>Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Vitamin D is a secosteroid hormone with many varied functions including regulation of blood calcium levels, cell proliferation, immunity, and reproduction in mammals. Vitamin D is activated by 25-hydroxylase (CYP2R1) and 1-alpha-hydroxylase (CYP27B1) and is degraded by 24-hydroxylase (CYP24A1). Vitamin D is transported by vitamin D-binding protein (group-specific component, GC) through the bloodstream and regulates cellular actions by binding to vitamin D receptor (VDR). In this study, we determined the expression and regulation of vitamin D-related molecules and the role of vitamin D at the maternal-conceptus interface in pigs. Vitamin D-metabolizing enzymes CYP2R1, CYP27B1, and CYP24A1, vitamin D binding protein GC, and vitamin D receptor VDR were expressed in the endometrium in a pregnancy stage-specific manner as well as in conceptus and chorioallantoic tissues during pregnancy. VDR protein was localized to endometrial and trophoblastic cells. Concentrations of calcitriol, the active form of vitamin D, in the endometrial tissues were higher during early pregnancy than in mid- to late pregnancy, while plasma concentrations of calcitriol were highest during late pregnancy. Furthermore, calcitriol affected the expression of several genes related to conceptus implantation, vitamin D metabolism, calcium ion regulation, PG metabolism, and calcium-binding proteins in endometrial tissue explants. These results show that CYP2R1, CYP27B1, CYP24A1, GC, and VDR were expressed at the maternal-conceptus interface, endometrial calcitriol levels were regulated during pregnancy, and calcitriol modulated the expression of endometrial genes, suggesting that calcitriol may play an important role in the establishment and maintenance of pregnancy by regulating endometrial function in pigs.</description><subject>25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism</subject><subject>Agricultural biotechnology</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Calcitriol</subject><subject>Calcium</subject><subject>Calcium (blood)</subject><subject>Calcium metabolism</subject><subject>Cell proliferation</subject><subject>Cholestanetriol 26-Monooxygenase - metabolism</subject><subject>Chorioallantoic Membrane - metabolism</subject><subject>Cytochrome P-450</subject><subject>Cytokines</subject><subject>Endometrium</subject><subject>Endometrium - metabolism</subject><subject>Endometrium - physiology</subject><subject>Enzymes</subject><subject>Explants</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes</subject><subject>Guanylate cyclase</subject><subject>Hogs</subject><subject>Homeostasis</subject><subject>Hydroxylase</subject><subject>Immunity</subject><subject>Implantation</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Physical sciences</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Pigs</subject><subject>Pregnancy</subject><subject>Protein binding</subject><subject>Proteins</subject><subject>Receptors, Calcitriol - metabolism</subject><subject>Rodents</subject><subject>Swine</subject><subject>Tissues</subject><subject>Vitamin D</subject><subject>Vitamin D - metabolism</subject><subject>Vitamin D - physiology</subject><subject>Vitamin D receptors</subject><subject>Vitamin D-binding protein</subject><subject>Vitamin D-Binding Protein - metabolism</subject><subject>Vitamin D3 24-Hydroxylase - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tuEzEQhlcIREvhDRBYQkJwkeDDHrlAqkqBSJUqceitNeudTVx57WB7q5bH4UlxmqQkqBfIF7bG3_y_PfZk2XNGp0xU7N2lG70FM106i1PK6opz9iA7ZI3gk5JT8XBnfZA9CeGS0kLUZfk4O-ANrWvesMPs94WOMGhLPk4GjNA6oxVB--tmwEDAdsSjgYgdGZxBNRoM78np9dJjCNpZApHEBZIhIavDTJSzCpdxDETbFOpB4a3KCvJJgbieXG0dE5OsOpeMvQZD5miR4F_xtL3U8_A0e9SDCfhsMx9lPz6dfj_5Mjk7_zw7OT6bqLLhcdLVNTBo27yvIW9BAFe9KmqgVQuMFSUXFeWlqhTWFesFNAWltKo7npdlVRWFOMpernWXxgW5KW-QrClqnldNVSVitiY6B5dy6fUA_kY60PI24Pxcgo9aGZQlE7xlqLhgkCMWdQ5Fsi6EqNLgTdL6sHEb2wE7hTZ6MHui-ztWL-TcXcmiLEUueBJ4sxHw7ueIIcpBB4XGgEU3rs9d5KykLKGv_kHvv92GmkO6gLa9S75qJSqPC8bzJhmvbKf3UGl0OOj0_NjrFN9LeLuXkJiI13EOYwhy9u3r_7PnF_vs6x12gWDiIjgzxvR1wj6Yr0HlXQge-7siMypXrbSthly1kty0Ukp7sftAd0nb3hF_AATrGqs</recordid><startdate>20171031</startdate><enddate>20171031</enddate><creator>Jang, Hwanhee</creator><creator>Choi, Yohan</creator><creator>Yoo, Inkyu</creator><creator>Han, Jisoo</creator><creator>Hong, Jin Su</creator><creator>Kim, Yoo Yong</creator><creator>Ka, Hakhyun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20171031</creationdate><title>Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs</title><author>Jang, Hwanhee ; Choi, Yohan ; Yoo, Inkyu ; Han, Jisoo ; Hong, Jin Su ; Kim, Yoo Yong ; Ka, Hakhyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d88a1abb4f8a4ba3a2cfc58a07ba1156237026c7ce871f3a9500078d246677553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism</topic><topic>Agricultural biotechnology</topic><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Calcitriol</topic><topic>Calcium</topic><topic>Calcium (blood)</topic><topic>Calcium metabolism</topic><topic>Cell proliferation</topic><topic>Cholestanetriol 26-Monooxygenase - metabolism</topic><topic>Chorioallantoic Membrane - metabolism</topic><topic>Cytochrome P-450</topic><topic>Cytokines</topic><topic>Endometrium</topic><topic>Endometrium - metabolism</topic><topic>Endometrium - physiology</topic><topic>Enzymes</topic><topic>Explants</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genes</topic><topic>Guanylate cyclase</topic><topic>Hogs</topic><topic>Homeostasis</topic><topic>Hydroxylase</topic><topic>Immunity</topic><topic>Implantation</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Physical sciences</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Pigs</topic><topic>Pregnancy</topic><topic>Protein binding</topic><topic>Proteins</topic><topic>Receptors, Calcitriol - metabolism</topic><topic>Rodents</topic><topic>Swine</topic><topic>Tissues</topic><topic>Vitamin D</topic><topic>Vitamin D - metabolism</topic><topic>Vitamin D - physiology</topic><topic>Vitamin D receptors</topic><topic>Vitamin D-binding protein</topic><topic>Vitamin D-Binding Protein - metabolism</topic><topic>Vitamin D3 24-Hydroxylase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jang, Hwanhee</creatorcontrib><creatorcontrib>Choi, Yohan</creatorcontrib><creatorcontrib>Yoo, Inkyu</creatorcontrib><creatorcontrib>Han, Jisoo</creatorcontrib><creatorcontrib>Hong, Jin Su</creatorcontrib><creatorcontrib>Kim, Yoo Yong</creatorcontrib><creatorcontrib>Ka, Hakhyun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jang, Hwanhee</au><au>Choi, Yohan</au><au>Yoo, Inkyu</au><au>Han, Jisoo</au><au>Hong, Jin Su</au><au>Kim, Yoo Yong</au><au>Ka, Hakhyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-10-31</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>e0187221</spage><epage>e0187221</epage><pages>e0187221-e0187221</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Vitamin D is a secosteroid hormone with many varied functions including regulation of blood calcium levels, cell proliferation, immunity, and reproduction in mammals. Vitamin D is activated by 25-hydroxylase (CYP2R1) and 1-alpha-hydroxylase (CYP27B1) and is degraded by 24-hydroxylase (CYP24A1). Vitamin D is transported by vitamin D-binding protein (group-specific component, GC) through the bloodstream and regulates cellular actions by binding to vitamin D receptor (VDR). In this study, we determined the expression and regulation of vitamin D-related molecules and the role of vitamin D at the maternal-conceptus interface in pigs. Vitamin D-metabolizing enzymes CYP2R1, CYP27B1, and CYP24A1, vitamin D binding protein GC, and vitamin D receptor VDR were expressed in the endometrium in a pregnancy stage-specific manner as well as in conceptus and chorioallantoic tissues during pregnancy. VDR protein was localized to endometrial and trophoblastic cells. Concentrations of calcitriol, the active form of vitamin D, in the endometrial tissues were higher during early pregnancy than in mid- to late pregnancy, while plasma concentrations of calcitriol were highest during late pregnancy. Furthermore, calcitriol affected the expression of several genes related to conceptus implantation, vitamin D metabolism, calcium ion regulation, PG metabolism, and calcium-binding proteins in endometrial tissue explants. These results show that CYP2R1, CYP27B1, CYP24A1, GC, and VDR were expressed at the maternal-conceptus interface, endometrial calcitriol levels were regulated during pregnancy, and calcitriol modulated the expression of endometrial genes, suggesting that calcitriol may play an important role in the establishment and maintenance of pregnancy by regulating endometrial function in pigs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29088291</pmid><doi>10.1371/journal.pone.0187221</doi><tpages>e0187221</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-10, Vol.12 (10), p.e0187221-e0187221 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1958247977 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism Agricultural biotechnology Animals Biology and Life Sciences Calcitriol Calcium Calcium (blood) Calcium metabolism Cell proliferation Cholestanetriol 26-Monooxygenase - metabolism Chorioallantoic Membrane - metabolism Cytochrome P-450 Cytokines Endometrium Endometrium - metabolism Endometrium - physiology Enzymes Explants Female Gene expression Gene Expression Regulation - physiology Genes Guanylate cyclase Hogs Homeostasis Hydroxylase Immunity Implantation Medicine and Health Sciences Metabolism Physical sciences Physiological aspects Physiology Pigs Pregnancy Protein binding Proteins Receptors, Calcitriol - metabolism Rodents Swine Tissues Vitamin D Vitamin D - metabolism Vitamin D - physiology Vitamin D receptors Vitamin D-binding protein Vitamin D-Binding Protein - metabolism Vitamin D3 24-Hydroxylase - metabolism |
| title | Vitamin D-metabolic enzymes and related molecules: Expression at the maternal-conceptus interface and the role of vitamin D in endometrial gene expression in pigs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T19%3A14%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Vitamin%20D-metabolic%20enzymes%20and%20related%20molecules:%20Expression%20at%20the%20maternal-conceptus%20interface%20and%20the%20role%20of%20vitamin%20D%20in%20endometrial%20gene%20expression%20in%20pigs&rft.jtitle=PloS%20one&rft.au=Jang,%20Hwanhee&rft.date=2017-10-31&rft.volume=12&rft.issue=10&rft.spage=e0187221&rft.epage=e0187221&rft.pages=e0187221-e0187221&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0187221&rft_dat=%3Cgale_plos_%3EA512496632%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1958247977&rft_id=info:pmid/29088291&rft_galeid=A512496632&rft_doaj_id=oai_doaj_org_article_6132b1ec231a4ee584a57ce533737329&rfr_iscdi=true |