Bioremediation of cooking oil waste using lipases from wastes
Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, alt...
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| creator | Okino-Delgado, Clarissa Hamaio Prado, Débora Zanoni do Facanali, Roselaine Marques, Márcia Mayo Ortiz Nascimento, Augusto Santana Fernandes, Célio Junior da Costa Zambuzzi, William Fernando Fleuri, Luciana Francisco |
| description | Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases. |
| doi_str_mv | 10.1371/journal.pone.0186246 |
| format | Article |
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In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0186246</identifier><identifier>PMID: 29073166</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alcohol ; Biocatalysts ; Biochemistry ; Biodegradation, Environmental ; Biology and Life Sciences ; Bioremediation ; Cell culture ; Cloning ; Commercialization ; Cooking ; Cooking oils ; Cytotoxicity ; Edible oils ; Edible vegetable oils ; Environmental aspects ; Environmental impact ; Enzymatic activity ; Enzymes ; Esterification ; Esters ; Fatty acids ; Fermentation ; Food science ; Frit ; Frying ; Gas chromatography ; Glycerol ; Lipase ; Lipase - metabolism ; Lipids ; Oil wastes ; Oils & fats ; Physical Sciences ; Soybean oil ; Soybean Oil - chemistry ; Soybeans ; Statistical analysis ; Studies ; Substrates ; Toxicity ; Toxicology ; Transesterification ; Waste management ; Waste treatment ; Wastes</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0186246-e0186246</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Okino-Delgado et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.</description><subject>Alcohol</subject><subject>Biocatalysts</subject><subject>Biochemistry</subject><subject>Biodegradation, Environmental</subject><subject>Biology and Life Sciences</subject><subject>Bioremediation</subject><subject>Cell culture</subject><subject>Cloning</subject><subject>Commercialization</subject><subject>Cooking</subject><subject>Cooking oils</subject><subject>Cytotoxicity</subject><subject>Edible oils</subject><subject>Edible vegetable oils</subject><subject>Environmental aspects</subject><subject>Environmental impact</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Esterification</subject><subject>Esters</subject><subject>Fatty acids</subject><subject>Fermentation</subject><subject>Food 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activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29073166</pmid><doi>10.1371/journal.pone.0186246</doi><tpages>e0186246</tpages><orcidid>https://orcid.org/0000-0002-8750-7298</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-10, Vol.12 (10), p.e0186246-e0186246 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1956438163 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alcohol Biocatalysts Biochemistry Biodegradation, Environmental Biology and Life Sciences Bioremediation Cell culture Cloning Commercialization Cooking Cooking oils Cytotoxicity Edible oils Edible vegetable oils Environmental aspects Environmental impact Enzymatic activity Enzymes Esterification Esters Fatty acids Fermentation Food science Frit Frying Gas chromatography Glycerol Lipase Lipase - metabolism Lipids Oil wastes Oils & fats Physical Sciences Soybean oil Soybean Oil - chemistry Soybeans Statistical analysis Studies Substrates Toxicity Toxicology Transesterification Waste management Waste treatment Wastes |
| title | Bioremediation of cooking oil waste using lipases from wastes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T14%3A16%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bioremediation%20of%20cooking%20oil%20waste%20using%20lipases%20from%20wastes&rft.jtitle=PloS%20one&rft.au=Okino-Delgado,%20Clarissa%20Hamaio&rft.date=2017-10-26&rft.volume=12&rft.issue=10&rft.spage=e0186246&rft.epage=e0186246&rft.pages=e0186246-e0186246&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0186246&rft_dat=%3Cgale_plos_%3EA511535420%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1956438163&rft_id=info:pmid/29073166&rft_galeid=A511535420&rft_doaj_id=oai_doaj_org_article_8d51fbfc3a0049c6bb74b240f8f0792e&rfr_iscdi=true |