The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium
Na+/K+-ATPase (NKA) is essential for maintaining the Na+ and K+ gradients, and supporting the secondary active transport of certain ions/molecules, across the plasma membrane of animal cells. This study aimed to clone the NKA α-subunit (NKAα) from the inner mantle adjacent to the extrapallial fluid...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-10, Vol.12 (10), p.e0186865-e0186865 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0186865 |
|---|---|
| container_issue | 10 |
| container_start_page | e0186865 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Boo, Mel V Hiong, Kum C Choo, Celine Y L Cao-Pham, Anh H Wong, Wai P Chew, Shit F Ip, Yuen K |
| description | Na+/K+-ATPase (NKA) is essential for maintaining the Na+ and K+ gradients, and supporting the secondary active transport of certain ions/molecules, across the plasma membrane of animal cells. This study aimed to clone the NKA α-subunit (NKAα) from the inner mantle adjacent to the extrapallial fluid of Tridacna squamosa, to determine its subcellular localization, and to examine the effects of light exposure on its transcript level and protein abundance. The cDNA coding sequence of NKAα from T. squamosa comprised 3105 bp, encoding 1034 amino acids with an estimated molecular mass of 114 kDa. NKAα had a basolateral localization along the shell-facing epithelium of the inner mantle. Exposure to 12 h of light led to a significantly stronger basolateral NKAα-immunofluorescence at the shell-facing epithelium, indicating that NKA might play a role in light-enhanced calcification in T. squamosa. After 3 h of light exposure, the transcript level of NKAα decreased transiently in the inner mantle, but returned to the control level thereafter. In comparison, the protein abundance of NKAα remained unchanged at hour 3, but became significantly higher than the control after 12 h of light exposure. Hence, the expression of NKAα in the inner mantle of T. squamosa was light-dependent. It is probable that a higher expression level of NKA was needed in the shell-facing epithelial cells of the inner mantle to cope with a rise in Na+ influx, possibly caused by increases in activities of some Na+-dependent ion transporters/channels involved in light-enhanced calcification. |
| doi_str_mv | 10.1371/journal.pone.0186865 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1953159720</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_5dbffabad8604709af58841d397e26ba</doaj_id><sourcerecordid>1954064403</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4415-6f389b8306487653b4f3eba019b991eb12e5398165d3d9e67c2ece31ea6d5e173</originalsourceid><addsrcrecordid>eNptUk1v1DAQjRCIloV_gMASF6Q22ziOnfhSqar4qKiAw3K2JvFk15Vjp3YC9GfxI7jym3C326pFnOI8v3kz8_yy7CUtlpTV9OjCz8GBXY7e4bKgjWgEf5TtU8nKXJQFe3zvvJc9i_GiKDhrhHia7ZWyqCQT9X72e7VBYpzDQAZwk0XiezIlbG3SL-ksDIdkFYyGzgGJlzMMPsIhwZ9jwBgxEiAtRG9hwgCWfIaDo08H-cnqK0Qkf37lcW5nZ6ZD8mNjug3RJo4WriKxZr2Zco0jOo2p0xodEnCajMFPaNxtB-MdAevdejtV3KC1eQ-dSQCOJmHWzMPz7EkPNuKL3XeRfXv_bnX6MT__8uHs9OQ876qK8lz0rJFtwwpRNbXgrK16hi0UVLZSUmxpiZzJhgqumZYo6q7EDhlFEJojrdkie32jO1of1e4FoqKSM8plnZxeZGc3DO3hQo3BDBCulAejtoAPawVhMp1FxXXb99CCbkRR1YWEnjdNRTWTNZaihaR1vOs2twPqLtmULH4g-vDGmY1a---Kp_1KLpLA251A8JczxkkNJnbJQXDo5-3cVfKiKliivvmH-v_tqhtWF3yMAfu7YWihrlN5W6WuU6l2qUxlr-4vcld0G0P2F6Z85JM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1953159720</pqid></control><display><type>article</type><title>The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Boo, Mel V ; Hiong, Kum C ; Choo, Celine Y L ; Cao-Pham, Anh H ; Wong, Wai P ; Chew, Shit F ; Ip, Yuen K</creator><contributor>Quintas, Luis Eduardo M</contributor><creatorcontrib>Boo, Mel V ; Hiong, Kum C ; Choo, Celine Y L ; Cao-Pham, Anh H ; Wong, Wai P ; Chew, Shit F ; Ip, Yuen K ; Quintas, Luis Eduardo M</creatorcontrib><description>Na+/K+-ATPase (NKA) is essential for maintaining the Na+ and K+ gradients, and supporting the secondary active transport of certain ions/molecules, across the plasma membrane of animal cells. This study aimed to clone the NKA α-subunit (NKAα) from the inner mantle adjacent to the extrapallial fluid of Tridacna squamosa, to determine its subcellular localization, and to examine the effects of light exposure on its transcript level and protein abundance. The cDNA coding sequence of NKAα from T. squamosa comprised 3105 bp, encoding 1034 amino acids with an estimated molecular mass of 114 kDa. NKAα had a basolateral localization along the shell-facing epithelium of the inner mantle. Exposure to 12 h of light led to a significantly stronger basolateral NKAα-immunofluorescence at the shell-facing epithelium, indicating that NKA might play a role in light-enhanced calcification in T. squamosa. After 3 h of light exposure, the transcript level of NKAα decreased transiently in the inner mantle, but returned to the control level thereafter. In comparison, the protein abundance of NKAα remained unchanged at hour 3, but became significantly higher than the control after 12 h of light exposure. Hence, the expression of NKAα in the inner mantle of T. squamosa was light-dependent. It is probable that a higher expression level of NKA was needed in the shell-facing epithelial cells of the inner mantle to cope with a rise in Na+ influx, possibly caused by increases in activities of some Na+-dependent ion transporters/channels involved in light-enhanced calcification.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0186865</identifier><identifier>PMID: 29049367</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Active transport ; Amino Acid Sequence ; Amino acids ; Animals ; Base Sequence ; Biology and Life Sciences ; Bivalvia - metabolism ; Blotting, Western ; Calcification ; Coral reefs ; DNA, Complementary ; Epithelial cells ; Epithelium ; Epithelium - metabolism ; Exposure ; Gene expression ; Immunofluorescence ; Light ; Light emitting diodes ; Localization ; Mantle ; Medicine and Health Sciences ; Microscopy, Fluorescence ; Mollusks ; Na+/K+-exchanging ATPase ; Nitrogen ; Photosynthesis ; Proteins ; Real-Time Polymerase Chain Reaction ; Research and Analysis Methods ; Respiration ; RNA, Messenger - genetics ; Science education ; Sequence Homology, Amino Acid ; Sodium-Potassium-Exchanging ATPase - chemistry ; Sodium-Potassium-Exchanging ATPase - genetics ; Sodium-Potassium-Exchanging ATPase - metabolism ; Transcription ; Tridacna squamosa</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0186865-e0186865</ispartof><rights>2017 Boo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Boo et al 2017 Boo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4415-6f389b8306487653b4f3eba019b991eb12e5398165d3d9e67c2ece31ea6d5e173</citedby><cites>FETCH-LOGICAL-c4415-6f389b8306487653b4f3eba019b991eb12e5398165d3d9e67c2ece31ea6d5e173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648256/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648256/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29049367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Quintas, Luis Eduardo M</contributor><creatorcontrib>Boo, Mel V</creatorcontrib><creatorcontrib>Hiong, Kum C</creatorcontrib><creatorcontrib>Choo, Celine Y L</creatorcontrib><creatorcontrib>Cao-Pham, Anh H</creatorcontrib><creatorcontrib>Wong, Wai P</creatorcontrib><creatorcontrib>Chew, Shit F</creatorcontrib><creatorcontrib>Ip, Yuen K</creatorcontrib><title>The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Na+/K+-ATPase (NKA) is essential for maintaining the Na+ and K+ gradients, and supporting the secondary active transport of certain ions/molecules, across the plasma membrane of animal cells. This study aimed to clone the NKA α-subunit (NKAα) from the inner mantle adjacent to the extrapallial fluid of Tridacna squamosa, to determine its subcellular localization, and to examine the effects of light exposure on its transcript level and protein abundance. The cDNA coding sequence of NKAα from T. squamosa comprised 3105 bp, encoding 1034 amino acids with an estimated molecular mass of 114 kDa. NKAα had a basolateral localization along the shell-facing epithelium of the inner mantle. Exposure to 12 h of light led to a significantly stronger basolateral NKAα-immunofluorescence at the shell-facing epithelium, indicating that NKA might play a role in light-enhanced calcification in T. squamosa. After 3 h of light exposure, the transcript level of NKAα decreased transiently in the inner mantle, but returned to the control level thereafter. In comparison, the protein abundance of NKAα remained unchanged at hour 3, but became significantly higher than the control after 12 h of light exposure. Hence, the expression of NKAα in the inner mantle of T. squamosa was light-dependent. It is probable that a higher expression level of NKA was needed in the shell-facing epithelial cells of the inner mantle to cope with a rise in Na+ influx, possibly caused by increases in activities of some Na+-dependent ion transporters/channels involved in light-enhanced calcification.</description><subject>Abundance</subject><subject>Active transport</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biology and Life Sciences</subject><subject>Bivalvia - metabolism</subject><subject>Blotting, Western</subject><subject>Calcification</subject><subject>Coral reefs</subject><subject>DNA, Complementary</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Epithelium - metabolism</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Immunofluorescence</subject><subject>Light</subject><subject>Light emitting diodes</subject><subject>Localization</subject><subject>Mantle</subject><subject>Medicine and Health Sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Mollusks</subject><subject>Na+/K+-exchanging ATPase</subject><subject>Nitrogen</subject><subject>Photosynthesis</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research and Analysis Methods</subject><subject>Respiration</subject><subject>RNA, Messenger - genetics</subject><subject>Science education</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sodium-Potassium-Exchanging ATPase - chemistry</subject><subject>Sodium-Potassium-Exchanging ATPase - genetics</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Transcription</subject><subject>Tridacna squamosa</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAQjRCIloV_gMASF6Q22ziOnfhSqar4qKiAw3K2JvFk15Vjp3YC9GfxI7jym3C326pFnOI8v3kz8_yy7CUtlpTV9OjCz8GBXY7e4bKgjWgEf5TtU8nKXJQFe3zvvJc9i_GiKDhrhHia7ZWyqCQT9X72e7VBYpzDQAZwk0XiezIlbG3SL-ksDIdkFYyGzgGJlzMMPsIhwZ9jwBgxEiAtRG9hwgCWfIaDo08H-cnqK0Qkf37lcW5nZ6ZD8mNjug3RJo4WriKxZr2Zco0jOo2p0xodEnCajMFPaNxtB-MdAevdejtV3KC1eQ-dSQCOJmHWzMPz7EkPNuKL3XeRfXv_bnX6MT__8uHs9OQ876qK8lz0rJFtwwpRNbXgrK16hi0UVLZSUmxpiZzJhgqumZYo6q7EDhlFEJojrdkie32jO1of1e4FoqKSM8plnZxeZGc3DO3hQo3BDBCulAejtoAPawVhMp1FxXXb99CCbkRR1YWEnjdNRTWTNZaihaR1vOs2twPqLtmULH4g-vDGmY1a---Kp_1KLpLA251A8JczxkkNJnbJQXDo5-3cVfKiKliivvmH-v_tqhtWF3yMAfu7YWihrlN5W6WuU6l2qUxlr-4vcld0G0P2F6Z85JM</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Boo, Mel V</creator><creator>Hiong, Kum C</creator><creator>Choo, Celine Y L</creator><creator>Cao-Pham, Anh H</creator><creator>Wong, Wai P</creator><creator>Chew, Shit F</creator><creator>Ip, Yuen K</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20171001</creationdate><title>The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium</title><author>Boo, Mel V ; Hiong, Kum C ; Choo, Celine Y L ; Cao-Pham, Anh H ; Wong, Wai P ; Chew, Shit F ; Ip, Yuen K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4415-6f389b8306487653b4f3eba019b991eb12e5398165d3d9e67c2ece31ea6d5e173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abundance</topic><topic>Active transport</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biology and Life Sciences</topic><topic>Bivalvia - metabolism</topic><topic>Blotting, Western</topic><topic>Calcification</topic><topic>Coral reefs</topic><topic>DNA, Complementary</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Epithelium - metabolism</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Immunofluorescence</topic><topic>Light</topic><topic>Light emitting diodes</topic><topic>Localization</topic><topic>Mantle</topic><topic>Medicine and Health Sciences</topic><topic>Microscopy, Fluorescence</topic><topic>Mollusks</topic><topic>Na+/K+-exchanging ATPase</topic><topic>Nitrogen</topic><topic>Photosynthesis</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Research and Analysis Methods</topic><topic>Respiration</topic><topic>RNA, Messenger - genetics</topic><topic>Science education</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sodium-Potassium-Exchanging ATPase - chemistry</topic><topic>Sodium-Potassium-Exchanging ATPase - genetics</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Transcription</topic><topic>Tridacna squamosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boo, Mel V</creatorcontrib><creatorcontrib>Hiong, Kum C</creatorcontrib><creatorcontrib>Choo, Celine Y L</creatorcontrib><creatorcontrib>Cao-Pham, Anh H</creatorcontrib><creatorcontrib>Wong, Wai P</creatorcontrib><creatorcontrib>Chew, Shit F</creatorcontrib><creatorcontrib>Ip, Yuen K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boo, Mel V</au><au>Hiong, Kum C</au><au>Choo, Celine Y L</au><au>Cao-Pham, Anh H</au><au>Wong, Wai P</au><au>Chew, Shit F</au><au>Ip, Yuen K</au><au>Quintas, Luis Eduardo M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>e0186865</spage><epage>e0186865</epage><pages>e0186865-e0186865</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Na+/K+-ATPase (NKA) is essential for maintaining the Na+ and K+ gradients, and supporting the secondary active transport of certain ions/molecules, across the plasma membrane of animal cells. This study aimed to clone the NKA α-subunit (NKAα) from the inner mantle adjacent to the extrapallial fluid of Tridacna squamosa, to determine its subcellular localization, and to examine the effects of light exposure on its transcript level and protein abundance. The cDNA coding sequence of NKAα from T. squamosa comprised 3105 bp, encoding 1034 amino acids with an estimated molecular mass of 114 kDa. NKAα had a basolateral localization along the shell-facing epithelium of the inner mantle. Exposure to 12 h of light led to a significantly stronger basolateral NKAα-immunofluorescence at the shell-facing epithelium, indicating that NKA might play a role in light-enhanced calcification in T. squamosa. After 3 h of light exposure, the transcript level of NKAα decreased transiently in the inner mantle, but returned to the control level thereafter. In comparison, the protein abundance of NKAα remained unchanged at hour 3, but became significantly higher than the control after 12 h of light exposure. Hence, the expression of NKAα in the inner mantle of T. squamosa was light-dependent. It is probable that a higher expression level of NKA was needed in the shell-facing epithelial cells of the inner mantle to cope with a rise in Na+ influx, possibly caused by increases in activities of some Na+-dependent ion transporters/channels involved in light-enhanced calcification.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29049367</pmid><doi>10.1371/journal.pone.0186865</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-10, Vol.12 (10), p.e0186865-e0186865 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1953159720 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abundance Active transport Amino Acid Sequence Amino acids Animals Base Sequence Biology and Life Sciences Bivalvia - metabolism Blotting, Western Calcification Coral reefs DNA, Complementary Epithelial cells Epithelium Epithelium - metabolism Exposure Gene expression Immunofluorescence Light Light emitting diodes Localization Mantle Medicine and Health Sciences Microscopy, Fluorescence Mollusks Na+/K+-exchanging ATPase Nitrogen Photosynthesis Proteins Real-Time Polymerase Chain Reaction Research and Analysis Methods Respiration RNA, Messenger - genetics Science education Sequence Homology, Amino Acid Sodium-Potassium-Exchanging ATPase - chemistry Sodium-Potassium-Exchanging ATPase - genetics Sodium-Potassium-Exchanging ATPase - metabolism Transcription Tridacna squamosa |
| title | The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T09%3A52%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20inner%20mantle%20of%20the%20giant%20clam,%20Tridacna%20squamosa,%20expresses%20a%20basolateral%20Na+/K+-ATPase%20%CE%B1-subunit,%20which%20displays%20light-dependent%20gene%20and%20protein%20expression%20along%20the%20shell-facing%20epithelium&rft.jtitle=PloS%20one&rft.au=Boo,%20Mel%20V&rft.date=2017-10-01&rft.volume=12&rft.issue=10&rft.spage=e0186865&rft.epage=e0186865&rft.pages=e0186865-e0186865&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0186865&rft_dat=%3Cproquest_plos_%3E1954064403%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1953159720&rft_id=info:pmid/29049367&rft_doaj_id=oai_doaj_org_article_5dbffabad8604709af58841d397e26ba&rfr_iscdi=true |