Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source
X-ray microbeam radiotherapy can potentially widen the therapeutic window due to a geometrical redistribution of the dose. However, high requirements on photon flux, beam collimation, and system stability restrict its application mainly to large-scale, cost-intensive synchrotron facilities. With a u...
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| creator | Burger, Karin Ilicic, Katarina Dierolf, Martin Günther, Benedikt Walsh, Dietrich W M Schmid, Ernst Eggl, Elena Achterhold, Klaus Gleich, Bernhard Combs, Stephanie E Molls, Michael Schmid, Thomas E Pfeiffer, Franz Wilkens, Jan J |
| description | X-ray microbeam radiotherapy can potentially widen the therapeutic window due to a geometrical redistribution of the dose. However, high requirements on photon flux, beam collimation, and system stability restrict its application mainly to large-scale, cost-intensive synchrotron facilities. With a unique laser-based Compact Light Source using inverse Compton scattering, we investigated the translation of this promising radiotherapy technique to a machine of future clinical relevance. We performed in vitro colony-forming assays and chromosome aberration tests in normal tissue cells after microbeam irradiation compared to homogeneous irradiation at the same mean dose using 25 keV X-rays. The microplanar pattern was achieved with a tungsten slit array of 50 μm slit size and a spacing of 350 μm. Applying microbeams significantly increased cell survival for a mean dose above 2 Gy, which indicates fewer normal tissue complications. The observation of significantly less chromosome aberrations suggests a lower risk of second cancer development. Our findings provide valuable insight into the mechanisms of microbeam radiotherapy and prove its applicability at a compact synchrotron, which contributes to its future clinical translation. |
| doi_str_mv | 10.1371/journal.pone.0186005 |
| format | Article |
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However, high requirements on photon flux, beam collimation, and system stability restrict its application mainly to large-scale, cost-intensive synchrotron facilities. With a unique laser-based Compact Light Source using inverse Compton scattering, we investigated the translation of this promising radiotherapy technique to a machine of future clinical relevance. We performed in vitro colony-forming assays and chromosome aberration tests in normal tissue cells after microbeam irradiation compared to homogeneous irradiation at the same mean dose using 25 keV X-rays. The microplanar pattern was achieved with a tungsten slit array of 50 μm slit size and a spacing of 350 μm. Applying microbeams significantly increased cell survival for a mean dose above 2 Gy, which indicates fewer normal tissue complications. The observation of significantly less chromosome aberrations suggests a lower risk of second cancer development. Our findings provide valuable insight into the mechanisms of microbeam radiotherapy and prove its applicability at a compact synchrotron, which contributes to its future clinical translation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0186005</identifier><identifier>PMID: 29049300</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aberration ; Animals ; Bioengineering ; Biology and Life Sciences ; Brain cancer ; Cancer ; Cell Survival ; CHO Cells ; Chromosome aberrations ; Chromosome Aberrations - radiation effects ; Chromosomes ; Collimation ; Complications ; Cricetulus ; Dosimetry ; Elastic scattering ; Engineering and Technology ; Feasibility studies ; Gene expression ; Health risks ; HeLa Cells ; Humans ; Irradiation ; Laser applications ; Lasers ; Light sources ; Medicine and Health Sciences ; Methods ; Microbeams ; Oncology ; Physical Sciences ; Physics ; Prevention ; Radiation exposure ; Radiation therapy ; Radiotherapy ; Survival ; Synchrotrons ; Translation ; Tungsten ; X ray sources ; X-Rays</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0186005-e0186005</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Burger et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Burger et al 2017 Burger et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-13aac23249f07e6345ef23b3629a66dad1563f93a1963b3cf092e00f381741523</citedby><cites>FETCH-LOGICAL-c692t-13aac23249f07e6345ef23b3629a66dad1563f93a1963b3cf092e00f381741523</cites><orcidid>0000-0001-8812-8065</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648152/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648152/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29049300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Woloschak, Gayle E.</contributor><creatorcontrib>Burger, Karin</creatorcontrib><creatorcontrib>Ilicic, Katarina</creatorcontrib><creatorcontrib>Dierolf, Martin</creatorcontrib><creatorcontrib>Günther, Benedikt</creatorcontrib><creatorcontrib>Walsh, Dietrich W M</creatorcontrib><creatorcontrib>Schmid, Ernst</creatorcontrib><creatorcontrib>Eggl, Elena</creatorcontrib><creatorcontrib>Achterhold, Klaus</creatorcontrib><creatorcontrib>Gleich, Bernhard</creatorcontrib><creatorcontrib>Combs, Stephanie E</creatorcontrib><creatorcontrib>Molls, Michael</creatorcontrib><creatorcontrib>Schmid, Thomas E</creatorcontrib><creatorcontrib>Pfeiffer, Franz</creatorcontrib><creatorcontrib>Wilkens, Jan J</creatorcontrib><title>Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>X-ray microbeam radiotherapy can potentially widen the therapeutic window due to a geometrical redistribution of the dose. 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cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source</title><author>Burger, Karin ; Ilicic, Katarina ; Dierolf, Martin ; Günther, Benedikt ; Walsh, Dietrich W M ; Schmid, Ernst ; Eggl, Elena ; Achterhold, Klaus ; Gleich, Bernhard ; Combs, Stephanie E ; Molls, Michael ; Schmid, Thomas E ; Pfeiffer, Franz ; Wilkens, Jan J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-13aac23249f07e6345ef23b3629a66dad1563f93a1963b3cf092e00f381741523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aberration</topic><topic>Animals</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Brain cancer</topic><topic>Cancer</topic><topic>Cell Survival</topic><topic>CHO Cells</topic><topic>Chromosome aberrations</topic><topic>Chromosome Aberrations - radiation 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One</addtitle><date>2017-10-19</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>e0186005</spage><epage>e0186005</epage><pages>e0186005-e0186005</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>X-ray microbeam radiotherapy can potentially widen the therapeutic window due to a geometrical redistribution of the dose. However, high requirements on photon flux, beam collimation, and system stability restrict its application mainly to large-scale, cost-intensive synchrotron facilities. With a unique laser-based Compact Light Source using inverse Compton scattering, we investigated the translation of this promising radiotherapy technique to a machine of future clinical relevance. We performed in vitro colony-forming assays and chromosome aberration tests in normal tissue cells after microbeam irradiation compared to homogeneous irradiation at the same mean dose using 25 keV X-rays. The microplanar pattern was achieved with a tungsten slit array of 50 μm slit size and a spacing of 350 μm. Applying microbeams significantly increased cell survival for a mean dose above 2 Gy, which indicates fewer normal tissue complications. The observation of significantly less chromosome aberrations suggests a lower risk of second cancer development. Our findings provide valuable insight into the mechanisms of microbeam radiotherapy and prove its applicability at a compact synchrotron, which contributes to its future clinical translation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29049300</pmid><doi>10.1371/journal.pone.0186005</doi><orcidid>https://orcid.org/0000-0001-8812-8065</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aberration Animals Bioengineering Biology and Life Sciences Brain cancer Cancer Cell Survival CHO Cells Chromosome aberrations Chromosome Aberrations - radiation effects Chromosomes Collimation Complications Cricetulus Dosimetry Elastic scattering Engineering and Technology Feasibility studies Gene expression Health risks HeLa Cells Humans Irradiation Laser applications Lasers Light sources Medicine and Health Sciences Methods Microbeams Oncology Physical Sciences Physics Prevention Radiation exposure Radiation therapy Radiotherapy Survival Synchrotrons Translation Tungsten X ray sources X-Rays |
| title | Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T02%3A10%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20cell%20survival%20and%20cytogenetic%20integrity%20by%20spatial%20dose%20redistribution%20at%20a%20compact%20synchrotron%20X-ray%20source&rft.jtitle=PloS%20one&rft.au=Burger,%20Karin&rft.date=2017-10-19&rft.volume=12&rft.issue=10&rft.spage=e0186005&rft.epage=e0186005&rft.pages=e0186005-e0186005&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0186005&rft_dat=%3Cgale_plos_%3EA510413451%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1953157467&rft_id=info:pmid/29049300&rft_galeid=A510413451&rft_doaj_id=oai_doaj_org_article_a34e201663e84cbc9c34a26664488bf7&rfr_iscdi=true |