A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location
Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a g...
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| Veröffentlicht in: | PloS one 2017, Vol.12 (10), p.e0186576-e0186576 |
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| description | Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media. After antimicrobial susceptibility testing and multi-locus variable number of tandem repeats assay (MLVA), selected ESBL-EC strains from recreational water were characterized by whole genome sequencing (WGS) and compared to wastewater and human urine isolates from people living in the same area. We detected ESBL-EC in recreational water samples on 8/20 occasions (40%), representing all sites. The ratio of ESBL-EC to total number of E. coli colony forming units varied from 0 to 3.8%. ESBL-EC were present in all wastewater samples in ratios of 0.56-0.75%. ST131 was most prevalent in urine and wastewater samples, while ST10 dominated in water samples. Eight STs and identical ESBL-EC MLVA-types were detected in all compartments. Clinical ESBL-EC isolates were more likely to be multidrug-resistant (p |
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The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media. After antimicrobial susceptibility testing and multi-locus variable number of tandem repeats assay (MLVA), selected ESBL-EC strains from recreational water were characterized by whole genome sequencing (WGS) and compared to wastewater and human urine isolates from people living in the same area. We detected ESBL-EC in recreational water samples on 8/20 occasions (40%), representing all sites. The ratio of ESBL-EC to total number of E. coli colony forming units varied from 0 to 3.8%. ESBL-EC were present in all wastewater samples in ratios of 0.56-0.75%. ST131 was most prevalent in urine and wastewater samples, while ST10 dominated in water samples. Eight STs and identical ESBL-EC MLVA-types were detected in all compartments. Clinical ESBL-EC isolates were more likely to be multidrug-resistant (p<0.001). This study confirms that ESBL-EC, including those that are capable of causing human infection, are present in recreational waters where there is a potential for human exposure and subsequent gut colonisation and infection in bathers. Multidrug-resistant E. coli strains are present in urban aquatic environments even in countries where antibiotic consumption in both humans and animals is highly restricted.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0186576</identifier><identifier>PMID: 29040337</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Aquatic environment ; Bacteria ; Basale biofag: 470 ; Basic biosciences: 470 ; Bathing Beaches ; Beaches ; beta-Lactamases - genetics ; Biology and Life Sciences ; Clinical medicine ; Colonization ; Comparative analysis ; Compartments ; Disease control ; Drug resistance ; Drug Resistance, Multiple, Bacterial ; E coli ; Earth Sciences ; Ecology and Environmental Sciences ; Enterobacteriaceae ; Epidemiological Monitoring ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - growth & development ; Escherichia coli - isolation & purification ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; Fresh Water - microbiology ; Gene Expression ; Gene sequencing ; General microbiology: 472 ; Generell mikrobiologi: 472 ; Genome, Bacterial ; Genomes ; High-Throughput Nucleotide Sequencing ; Hospitals ; Human wastes ; Humans ; Infections ; Laboratories ; Matematikk og Naturvitenskap: 400 ; Mathematics and natural science: 400 ; Medicine ; Medicine and Health Sciences ; Microbial Sensitivity Tests ; Multidrug resistance ; Multilocus Sequence Typing ; Norway - epidemiology ; People and Places ; Recreation ; Recreational waters ; Selective media ; Sewage ; Sewage treatment plants ; Studies ; Surveillance ; Urinary tract diseases ; Urinary tract infections ; Urinary Tract Infections - epidemiology ; Urinary Tract Infections - microbiology ; Urine ; Urogenital system ; VDP ; Waste Water - microbiology ; Wastewater ; Wastewater treatment plants ; Water analysis ; Water Microbiology ; Water quality ; Water sampling ; Water treatment</subject><ispartof>PloS one, 2017, Vol.12 (10), p.e0186576-e0186576</ispartof><rights>2017 Jørgensen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>info:eu-repo/semantics/openAccess</rights><rights>2017 Jørgensen et al 2017 Jørgensen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-e4378fdac21179368fda4e40782826b11a5aa82debcf5c9a4a4aafc6fda5da763</citedby><cites>FETCH-LOGICAL-c550t-e4378fdac21179368fda4e40782826b11a5aa82debcf5c9a4a4aafc6fda5da763</cites><orcidid>0000-0002-3135-1431</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645111/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645111/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,4010,23845,26544,27900,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29040337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jørgensen, Silje B</creatorcontrib><creatorcontrib>Søraas, Arne V</creatorcontrib><creatorcontrib>Arnesen, Lotte S</creatorcontrib><creatorcontrib>Leegaard, Truls M</creatorcontrib><creatorcontrib>Sundsfjord, Arnfinn</creatorcontrib><creatorcontrib>Jenum, Pål A</creatorcontrib><title>A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media. After antimicrobial susceptibility testing and multi-locus variable number of tandem repeats assay (MLVA), selected ESBL-EC strains from recreational water were characterized by whole genome sequencing (WGS) and compared to wastewater and human urine isolates from people living in the same area. We detected ESBL-EC in recreational water samples on 8/20 occasions (40%), representing all sites. The ratio of ESBL-EC to total number of E. coli colony forming units varied from 0 to 3.8%. ESBL-EC were present in all wastewater samples in ratios of 0.56-0.75%. ST131 was most prevalent in urine and wastewater samples, while ST10 dominated in water samples. Eight STs and identical ESBL-EC MLVA-types were detected in all compartments. Clinical ESBL-EC isolates were more likely to be multidrug-resistant (p<0.001). This study confirms that ESBL-EC, including those that are capable of causing human infection, are present in recreational waters where there is a potential for human exposure and subsequent gut colonisation and infection in bathers. Multidrug-resistant E. coli strains are present in urban aquatic environments even in countries where antibiotic consumption in both humans and animals is highly restricted.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Aquatic environment</subject><subject>Bacteria</subject><subject>Basale biofag: 470</subject><subject>Basic biosciences: 470</subject><subject>Bathing Beaches</subject><subject>Beaches</subject><subject>beta-Lactamases - genetics</subject><subject>Biology and Life Sciences</subject><subject>Clinical medicine</subject><subject>Colonization</subject><subject>Comparative analysis</subject><subject>Compartments</subject><subject>Disease control</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>E coli</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Enterobacteriaceae</subject><subject>Epidemiological Monitoring</subject><subject>Escherichia coli</subject><subject>Escherichia coli - 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Academic</collection><collection>NORA - Norwegian Open Research Archives</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jørgensen, Silje B</au><au>Søraas, Arne V</au><au>Arnesen, Lotte S</au><au>Leegaard, Truls M</au><au>Sundsfjord, Arnfinn</au><au>Jenum, Pål A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>e0186576</spage><epage>e0186576</epage><pages>e0186576-e0186576</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media. After antimicrobial susceptibility testing and multi-locus variable number of tandem repeats assay (MLVA), selected ESBL-EC strains from recreational water were characterized by whole genome sequencing (WGS) and compared to wastewater and human urine isolates from people living in the same area. We detected ESBL-EC in recreational water samples on 8/20 occasions (40%), representing all sites. The ratio of ESBL-EC to total number of E. coli colony forming units varied from 0 to 3.8%. ESBL-EC were present in all wastewater samples in ratios of 0.56-0.75%. ST131 was most prevalent in urine and wastewater samples, while ST10 dominated in water samples. Eight STs and identical ESBL-EC MLVA-types were detected in all compartments. Clinical ESBL-EC isolates were more likely to be multidrug-resistant (p<0.001). This study confirms that ESBL-EC, including those that are capable of causing human infection, are present in recreational waters where there is a potential for human exposure and subsequent gut colonisation and infection in bathers. Multidrug-resistant E. coli strains are present in urban aquatic environments even in countries where antibiotic consumption in both humans and animals is highly restricted.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29040337</pmid><doi>10.1371/journal.pone.0186576</doi><orcidid>https://orcid.org/0000-0002-3135-1431</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017, Vol.12 (10), p.e0186576-e0186576 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1952048114 |
| source | MEDLINE; NORA - Norwegian Open Research Archives; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Aquatic environment Bacteria Basale biofag: 470 Basic biosciences: 470 Bathing Beaches Beaches beta-Lactamases - genetics Biology and Life Sciences Clinical medicine Colonization Comparative analysis Compartments Disease control Drug resistance Drug Resistance, Multiple, Bacterial E coli Earth Sciences Ecology and Environmental Sciences Enterobacteriaceae Epidemiological Monitoring Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Fresh Water - microbiology Gene Expression Gene sequencing General microbiology: 472 Generell mikrobiologi: 472 Genome, Bacterial Genomes High-Throughput Nucleotide Sequencing Hospitals Human wastes Humans Infections Laboratories Matematikk og Naturvitenskap: 400 Mathematics and natural science: 400 Medicine Medicine and Health Sciences Microbial Sensitivity Tests Multidrug resistance Multilocus Sequence Typing Norway - epidemiology People and Places Recreation Recreational waters Selective media Sewage Sewage treatment plants Studies Surveillance Urinary tract diseases Urinary tract infections Urinary Tract Infections - epidemiology Urinary Tract Infections - microbiology Urine Urogenital system VDP Waste Water - microbiology Wastewater Wastewater treatment plants Water analysis Water Microbiology Water quality Water sampling Water treatment |
| title | A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location |
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