Response to: Comment on Rohrscheib et al. 2016 "Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes"

About the Authors: Chelsie E. Rohrscheib Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Francesca D. Frentiu Affiliation: Institute of Health and Biomedical Innovation and School...

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Veröffentlicht in:PLoS pathogens 2017-09, Vol.13 (9), p.e1006521-e1006521
Hauptverfasser: Rohrscheib, Chelsie E, Frentiu, Francesca D, Horn, Emilie, Ritchie, Fiona K, van Swinderen, Bruno, Weible, 2nd, Michael W, O'Neill, Scott L, Brownlie, Jeremy C
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container_end_page e1006521
container_issue 9
container_start_page e1006521
container_title PLoS pathogens
container_volume 13
creator Rohrscheib, Chelsie E
Frentiu, Francesca D
Horn, Emilie
Ritchie, Fiona K
van Swinderen, Bruno
Weible, 2nd, Michael W
O'Neill, Scott L
Brownlie, Jeremy C
description About the Authors: Chelsie E. Rohrscheib Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Francesca D. Frentiu Affiliation: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Australia Emilie Horn Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Fiona K. Ritchie Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Bruno van Swinderen Affiliation: Queensland Brain Institute, The University of Queensland, St. Lucia, Australia Michael W. Weible II Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Scott L. O’Neill Affiliation: School of Biological Sciences, Monash University, Clayton, VIC, Australia Jeremy C. Brownlie * E-mail: j.brownlie@griffith.edu.au Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Environmental Futures Research Institute, Griffith University, Nathan, Australia ORCID http://orcid.org/0000-0003-0161-2204Citation: Rohrscheib CE, Frentiu FD, Horn E, Ritchie FK, van Swinderen B, Weible MW II, et al. [...]we conclude that our qPCR assay was valid and our previous estimates of Octomom copy number correct. In their commentary Chrostek and Teixeira conclude from this model that “flies carrying wMelPop with high Octomom copy numbers die faster and, therefore, at later time points these Wolbachia are depleted from the pool of total wMelPop and mean Octomom copy number decreases.” Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines.
doi_str_mv 10.1371/journal.ppat.1006521
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Rohrscheib Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Francesca D. Frentiu Affiliation: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Australia Emilie Horn Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Fiona K. Ritchie Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Bruno van Swinderen Affiliation: Queensland Brain Institute, The University of Queensland, St. Lucia, Australia Michael W. Weible II Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Scott L. O’Neill Affiliation: School of Biological Sciences, Monash University, Clayton, VIC, Australia Jeremy C. Brownlie * E-mail: j.brownlie@griffith.edu.au Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Environmental Futures Research Institute, Griffith University, Nathan, Australia ORCID http://orcid.org/0000-0003-0161-2204Citation: Rohrscheib CE, Frentiu FD, Horn E, Ritchie FK, van Swinderen B, Weible MW II, et al. [...]we conclude that our qPCR assay was valid and our previous estimates of Octomom copy number correct. In their commentary Chrostek and Teixeira conclude from this model that “flies carrying wMelPop with high Octomom copy numbers die faster and, therefore, at later time points these Wolbachia are depleted from the pool of total wMelPop and mean Octomom copy number decreases.” Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1006521</identifier><identifier>PMID: 28892518</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aquatic insects ; Awards &amp; honors ; Biology ; Biology and Life Sciences ; Breakdowns ; Drosophila ; Drosophila melanogaster ; Evolution ; Experiments ; Formal Comment ; Gene expression ; Genetic engineering ; Genome, Bacterial ; Genomes ; Insects ; Medical innovations ; Medicine and Health Sciences ; Mutualism ; Pathology ; Phylogeny ; R&amp;D ; Research &amp; development ; Research and Analysis Methods ; Studies ; Symbiosis ; Temperature ; Wolbachia ; Wolbachia - genetics</subject><ispartof>PLoS pathogens, 2017-09, Vol.13 (9), p.e1006521-e1006521</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: genes". PLoS Pathog13(9): e1006521. https://doi.org/10.1371/journal.ppat.1006521</rights><rights>2017 Rohrscheib et al 2017 Rohrscheib et al</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: genes". 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Rohrscheib Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Francesca D. Frentiu Affiliation: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Australia Emilie Horn Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Fiona K. Ritchie Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Bruno van Swinderen Affiliation: Queensland Brain Institute, The University of Queensland, St. Lucia, Australia Michael W. Weible II Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Scott L. O’Neill Affiliation: School of Biological Sciences, Monash University, Clayton, VIC, Australia Jeremy C. Brownlie * E-mail: j.brownlie@griffith.edu.au Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Environmental Futures Research Institute, Griffith University, Nathan, Australia ORCID http://orcid.org/0000-0003-0161-2204Citation: Rohrscheib CE, Frentiu FD, Horn E, Ritchie FK, van Swinderen B, Weible MW II, et al. [...]we conclude that our qPCR assay was valid and our previous estimates of Octomom copy number correct. In their commentary Chrostek and Teixeira conclude from this model that “flies carrying wMelPop with high Octomom copy numbers die faster and, therefore, at later time points these Wolbachia are depleted from the pool of total wMelPop and mean Octomom copy number decreases.” Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines.</description><subject>Aquatic insects</subject><subject>Awards &amp; honors</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Breakdowns</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Evolution</subject><subject>Experiments</subject><subject>Formal Comment</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Insects</subject><subject>Medical innovations</subject><subject>Medicine and Health Sciences</subject><subject>Mutualism</subject><subject>Pathology</subject><subject>Phylogeny</subject><subject>R&amp;D</subject><subject>Research &amp; 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Rohrscheib Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Francesca D. Frentiu Affiliation: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Australia Emilie Horn Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Fiona K. Ritchie Affiliation: School of Natural Sciences, Griffith University, Nathan, Australia Bruno van Swinderen Affiliation: Queensland Brain Institute, The University of Queensland, St. Lucia, Australia Michael W. Weible II Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Griffith Research Institute for Drug Discovery, Griffith University, Nathan, Australia Scott L. O’Neill Affiliation: School of Biological Sciences, Monash University, Clayton, VIC, Australia Jeremy C. Brownlie * E-mail: j.brownlie@griffith.edu.au Affiliations School of Natural Sciences, Griffith University, Nathan, Australia, Environmental Futures Research Institute, Griffith University, Nathan, Australia ORCID http://orcid.org/0000-0003-0161-2204Citation: Rohrscheib CE, Frentiu FD, Horn E, Ritchie FK, van Swinderen B, Weible MW II, et al. [...]we conclude that our qPCR assay was valid and our previous estimates of Octomom copy number correct. In their commentary Chrostek and Teixeira conclude from this model that “flies carrying wMelPop with high Octomom copy numbers die faster and, therefore, at later time points these Wolbachia are depleted from the pool of total wMelPop and mean Octomom copy number decreases.” Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28892518</pmid><doi>10.1371/journal.ppat.1006521</doi><orcidid>https://orcid.org/0000-0003-0161-2204</orcidid><oa>free_for_read</oa></addata></record>
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subjects Aquatic insects
Awards & honors
Biology
Biology and Life Sciences
Breakdowns
Drosophila
Drosophila melanogaster
Evolution
Experiments
Formal Comment
Gene expression
Genetic engineering
Genome, Bacterial
Genomes
Insects
Medical innovations
Medicine and Health Sciences
Mutualism
Pathology
Phylogeny
R&D
Research & development
Research and Analysis Methods
Studies
Symbiosis
Temperature
Wolbachia
Wolbachia - genetics
title Response to: Comment on Rohrscheib et al. 2016 "Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes"
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