A single gp120 residue can affect HIV-1 tropism in macaques
Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express H...
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| Veröffentlicht in: | PLoS pathogens 2017-09, Vol.13 (9), p.e1006572 |
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| creator | Del Prete, Gregory Q Keele, Brandon F Fode, Jeannine Thummar, Keyur Swanstrom, Adrienne E Rodriguez, Anthony Raymond, Alice Estes, Jacob D LaBranche, Celia C Montefiori, David C KewalRamani, Vineet N Lifson, Jeffrey D Bieniasz, Paul D Hatziioannou, Theodora |
| description | Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1. |
| doi_str_mv | 10.1371/journal.ppat.1006572 |
| format | Article |
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Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1006572</identifier><identifier>PMID: 28945790</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; Adaptation ; Adaptation, Physiological - physiology ; AIDS ; Amino acids ; Animal models ; Animals ; Binding sites ; Biology and life sciences ; Biomedical research ; Cancer ; Care and treatment ; CD4 antigen ; CD4 Antigens - metabolism ; Disease Models, Animal ; Female ; Flow Cytometry ; Glycoprotein gp120 ; Glycoproteins ; HIV ; HIV Envelope Protein gp120 - chemistry ; HIV Envelope Protein gp120 - genetics ; HIV Infections - genetics ; HIV Infections - virology ; HIV-1 - physiology ; Human immunodeficiency virus ; Humans ; Immunoblotting ; Infections ; Laboratories ; Macaca mulatta ; Macaca nemestrina ; Macaques ; Male ; Medical research ; Medicine and Health Sciences ; Monkeys & apes ; Mutation ; Neutralization ; Physiological aspects ; Polymerase Chain Reaction ; Prevention ; Proteins ; Reagents ; Research and Analysis Methods ; Simian Immunodeficiency Virus ; Surgery ; Tropism ; Viral Tropism - physiology ; Virology ; Virus replication ; Viruses</subject><ispartof>PLoS pathogens, 2017-09, Vol.13 (9), p.e1006572</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Del Prete GQ, Keele BF, Fode J, Thummar K, Swanstrom AE, Rodriguez A, et al. (2017) A single gp120 residue can affect HIV-1 tropism in macaques. PLoS Pathog13(9): e1006572. https://doi.org/10.1371/journal.ppat.1006572</rights><rights>2017 Del Prete et al 2017 Del Prete et al</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Del Prete GQ, Keele BF, Fode J, Thummar K, Swanstrom AE, Rodriguez A, et al. (2017) A single gp120 residue can affect HIV-1 tropism in macaques. PLoS Pathog13(9): e1006572. https://doi.org/10.1371/journal.ppat.1006572</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c661t-3e89e222c532b6e5ecde0ca4c3be5a88352f62752a1f9444b260fc86334c3d753</citedby><cites>FETCH-LOGICAL-c661t-3e89e222c532b6e5ecde0ca4c3be5a88352f62752a1f9444b260fc86334c3d753</cites><orcidid>0000-0002-2381-1151 ; 0000-0002-2368-3719 ; 0000-0002-6653-6655 ; 0000-0003-3430-235X ; 0000-0001-6816-5870 ; 0000-0002-9494-0268 ; 0000-0002-7889-0766 ; 0000-0003-0429-4213 ; 0000-0001-6180-5361 ; 0000-0002-3148-9764</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5629034/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5629034/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28945790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Trkola, Alexandra</contributor><creatorcontrib>Del Prete, Gregory Q</creatorcontrib><creatorcontrib>Keele, Brandon F</creatorcontrib><creatorcontrib>Fode, Jeannine</creatorcontrib><creatorcontrib>Thummar, Keyur</creatorcontrib><creatorcontrib>Swanstrom, Adrienne E</creatorcontrib><creatorcontrib>Rodriguez, Anthony</creatorcontrib><creatorcontrib>Raymond, Alice</creatorcontrib><creatorcontrib>Estes, Jacob D</creatorcontrib><creatorcontrib>LaBranche, Celia C</creatorcontrib><creatorcontrib>Montefiori, David C</creatorcontrib><creatorcontrib>KewalRamani, Vineet N</creatorcontrib><creatorcontrib>Lifson, Jeffrey D</creatorcontrib><creatorcontrib>Bieniasz, Paul D</creatorcontrib><creatorcontrib>Hatziioannou, Theodora</creatorcontrib><title>A single gp120 residue can affect HIV-1 tropism in macaques</title><title>PLoS pathogens</title><addtitle>PLoS Pathog</addtitle><description>Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1.</description><subject>Acquired immune deficiency syndrome</subject><subject>Adaptation</subject><subject>Adaptation, Physiological - physiology</subject><subject>AIDS</subject><subject>Amino acids</subject><subject>Animal models</subject><subject>Animals</subject><subject>Binding sites</subject><subject>Biology and life sciences</subject><subject>Biomedical research</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>CD4 antigen</subject><subject>CD4 Antigens - metabolism</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Glycoprotein gp120</subject><subject>Glycoproteins</subject><subject>HIV</subject><subject>HIV Envelope Protein gp120 - chemistry</subject><subject>HIV Envelope Protein gp120 - genetics</subject><subject>HIV Infections - genetics</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - physiology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Macaca mulatta</subject><subject>Macaca nemestrina</subject><subject>Macaques</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Monkeys & apes</subject><subject>Mutation</subject><subject>Neutralization</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction</subject><subject>Prevention</subject><subject>Proteins</subject><subject>Reagents</subject><subject>Research and Analysis Methods</subject><subject>Simian Immunodeficiency Virus</subject><subject>Surgery</subject><subject>Tropism</subject><subject>Viral Tropism - 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single gp120 residue can affect HIV-1 tropism in macaques</title><author>Del Prete, Gregory Q ; Keele, Brandon F ; Fode, Jeannine ; Thummar, Keyur ; Swanstrom, Adrienne E ; Rodriguez, Anthony ; Raymond, Alice ; Estes, Jacob D ; LaBranche, Celia C ; Montefiori, David C ; KewalRamani, Vineet N ; Lifson, Jeffrey D ; Bieniasz, Paul D ; Hatziioannou, Theodora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c661t-3e89e222c532b6e5ecde0ca4c3be5a88352f62752a1f9444b260fc86334c3d753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>Adaptation</topic><topic>Adaptation, Physiological - physiology</topic><topic>AIDS</topic><topic>Amino acids</topic><topic>Animal models</topic><topic>Animals</topic><topic>Binding sites</topic><topic>Biology and life sciences</topic><topic>Biomedical research</topic><topic>Cancer</topic><topic>Care and 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Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. 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| ispartof | PLoS pathogens, 2017-09, Vol.13 (9), p.e1006572 |
| issn | 1553-7374 1553-7366 1553-7374 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acquired immune deficiency syndrome Adaptation Adaptation, Physiological - physiology AIDS Amino acids Animal models Animals Binding sites Biology and life sciences Biomedical research Cancer Care and treatment CD4 antigen CD4 Antigens - metabolism Disease Models, Animal Female Flow Cytometry Glycoprotein gp120 Glycoproteins HIV HIV Envelope Protein gp120 - chemistry HIV Envelope Protein gp120 - genetics HIV Infections - genetics HIV Infections - virology HIV-1 - physiology Human immunodeficiency virus Humans Immunoblotting Infections Laboratories Macaca mulatta Macaca nemestrina Macaques Male Medical research Medicine and Health Sciences Monkeys & apes Mutation Neutralization Physiological aspects Polymerase Chain Reaction Prevention Proteins Reagents Research and Analysis Methods Simian Immunodeficiency Virus Surgery Tropism Viral Tropism - physiology Virology Virus replication Viruses |
| title | A single gp120 residue can affect HIV-1 tropism in macaques |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A26%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20single%20gp120%20residue%20can%20affect%20HIV-1%20tropism%20in%20macaques&rft.jtitle=PLoS%20pathogens&rft.au=Del%20Prete,%20Gregory%20Q&rft.date=2017-09-01&rft.volume=13&rft.issue=9&rft.spage=e1006572&rft.pages=e1006572-&rft.issn=1553-7374&rft.eissn=1553-7374&rft_id=info:doi/10.1371/journal.ppat.1006572&rft_dat=%3Cgale_plos_%3EA507384834%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1951452394&rft_id=info:pmid/28945790&rft_galeid=A507384834&rft_doaj_id=oai_doaj_org_article_6b49d622424547a9829eab904952bf7a&rfr_iscdi=true |