Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV chall...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-09, Vol.12 (9), p.e0185184-e0185184 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0185184 |
|---|---|
| container_issue | 9 |
| container_start_page | e0185184 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Gammella, Mariela Digiacomo, Sebastian Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia |
| description | Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential. |
| doi_str_mv | 10.1371/journal.pone.0185184 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1943088110</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A506665048</galeid><doaj_id>oai_doaj_org_article_799498b29351497bb64c99928e4f1158</doaj_id><sourcerecordid>A506665048</sourcerecordid><originalsourceid>FETCH-LOGICAL-c734t-af9170340d6fbb9c62215e5c5e10bef283c9082624ed77d9da1d273a49d848113</originalsourceid><addsrcrecordid>eNqNk11r2zAUhs3YWLtu_2BshsHYLpLpy7J0MyjdV6BQ2BfsSsjScaLgWK4kl-3fT2nckIxeDGMsy8_7yueVTlE8x2iOaY3frf0Yet3NB9_DHGFRYcEeFKdYUjLjBNGHB-OT4kmMa4QqKjh_XJwQIZmUUpwWvz5Ab4PbQHCmHGBIzkIsje5L4_sWQjkEn8Ak5_tSL7XrYypb79NM93a28WNaldZF0BHKGxfGWLqs1Cl18LR41OouwrPpeVb8-PTx-8WX2eXV58XF-eXM1JRln1biGlGGLG-bRhpOCK6gMhVg1EBLBDUSCcIJA1vXVlqNLampZtIKJjCmZ8XLne_Q-aimVKLCklEkMoAysdgR1uu1GnK1OvxRXjt1O-HDUumQnOlA1TIHIxoiaYWZrJuGM5ODIgJYi3Elstf7abWx2YA10KeguyPT4y-9W6mlv1EVx6zidTbAOwMTR6MCGAg5r1vh_mV7E1QTRTETgmTNm2nR4K9HiEltXDTQdboHP-5q5YznjDL66h_0_kQmaqlz0a5vff5XszVV5xXinFeIbWud30Ply8LG5eMBrcvzR4K3R4LMJPidlnqMUS2-ff1_9urnMfv6gF2B7tIq-m7cnsp4DLIp3OBjDNDuNwYjte2auzTUtmvU1DVZ9uJwU_eiuzahfwFXHhCV</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1943088110</pqid></control><display><type>article</type><title>Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Recercat</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Soria, Ivana ; Quattrocchi, Valeria ; Langellotti, Cecilia ; Gammella, Mariela ; Digiacomo, Sebastian ; Garcia de la Torre, Beatriz ; Andreu, David ; Montoya, Maria ; Sobrino, Francisco ; Blanco, Esther ; Zamorano, Patricia</creator><creatorcontrib>Soria, Ivana ; Quattrocchi, Valeria ; Langellotti, Cecilia ; Gammella, Mariela ; Digiacomo, Sebastian ; Garcia de la Torre, Beatriz ; Andreu, David ; Montoya, Maria ; Sobrino, Francisco ; Blanco, Esther ; Zamorano, Patricia</creatorcontrib><description>Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0185184</identifier><identifier>PMID: 28949998</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies ; Biology and Life Sciences ; Cattle ; Dendrimers ; Dendrimers - pharmacology ; Epitopes ; Foot & mouth disease ; Foot and mouth disease ; Foot-and-Mouth Disease - prevention & control ; Foot-and-Mouth Disease Virus - immunology ; Immune response ; Immunization ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulins ; Immunology ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Medicine and Health Sciences ; Mucosa ; Peptides ; Peptides - pharmacology ; Pigs ; Prevention ; Proteins ; Research and Analysis Methods ; Secretions ; Swine ; Vaccines ; Viral Vaccines ; Viruses ; VP1 protein</subject><ispartof>PloS one, 2017-09, Vol.12 (9), p.e0185184-e0185184</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Soria et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Soria et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</a></rights><rights>2017 Soria et al 2017 Soria et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c734t-af9170340d6fbb9c62215e5c5e10bef283c9082624ed77d9da1d273a49d848113</citedby><cites>FETCH-LOGICAL-c734t-af9170340d6fbb9c62215e5c5e10bef283c9082624ed77d9da1d273a49d848113</cites><orcidid>0000-0002-0245-8993</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614567/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614567/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,26951,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28949998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soria, Ivana</creatorcontrib><creatorcontrib>Quattrocchi, Valeria</creatorcontrib><creatorcontrib>Langellotti, Cecilia</creatorcontrib><creatorcontrib>Gammella, Mariela</creatorcontrib><creatorcontrib>Digiacomo, Sebastian</creatorcontrib><creatorcontrib>Garcia de la Torre, Beatriz</creatorcontrib><creatorcontrib>Andreu, David</creatorcontrib><creatorcontrib>Montoya, Maria</creatorcontrib><creatorcontrib>Sobrino, Francisco</creatorcontrib><creatorcontrib>Blanco, Esther</creatorcontrib><creatorcontrib>Zamorano, Patricia</creatorcontrib><title>Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Biology and Life Sciences</subject><subject>Cattle</subject><subject>Dendrimers</subject><subject>Dendrimers - pharmacology</subject><subject>Epitopes</subject><subject>Foot & mouth disease</subject><subject>Foot and mouth disease</subject><subject>Foot-and-Mouth Disease - prevention & control</subject><subject>Foot-and-Mouth Disease Virus - immunology</subject><subject>Immune response</subject><subject>Immunization</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Immunology</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Medicine and Health Sciences</subject><subject>Mucosa</subject><subject>Peptides</subject><subject>Peptides - pharmacology</subject><subject>Pigs</subject><subject>Prevention</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Secretions</subject><subject>Swine</subject><subject>Vaccines</subject><subject>Viral Vaccines</subject><subject>Viruses</subject><subject>VP1 protein</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>XX2</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11r2zAUhs3YWLtu_2BshsHYLpLpy7J0MyjdV6BQ2BfsSsjScaLgWK4kl-3fT2nckIxeDGMsy8_7yueVTlE8x2iOaY3frf0Yet3NB9_DHGFRYcEeFKdYUjLjBNGHB-OT4kmMa4QqKjh_XJwQIZmUUpwWvz5Ab4PbQHCmHGBIzkIsje5L4_sWQjkEn8Ak5_tSL7XrYypb79NM93a28WNaldZF0BHKGxfGWLqs1Cl18LR41OouwrPpeVb8-PTx-8WX2eXV58XF-eXM1JRln1biGlGGLG-bRhpOCK6gMhVg1EBLBDUSCcIJA1vXVlqNLampZtIKJjCmZ8XLne_Q-aimVKLCklEkMoAysdgR1uu1GnK1OvxRXjt1O-HDUumQnOlA1TIHIxoiaYWZrJuGM5ODIgJYi3Elstf7abWx2YA10KeguyPT4y-9W6mlv1EVx6zidTbAOwMTR6MCGAg5r1vh_mV7E1QTRTETgmTNm2nR4K9HiEltXDTQdboHP-5q5YznjDL66h_0_kQmaqlz0a5vff5XszVV5xXinFeIbWud30Ply8LG5eMBrcvzR4K3R4LMJPidlnqMUS2-ff1_9urnMfv6gF2B7tIq-m7cnsp4DLIp3OBjDNDuNwYjte2auzTUtmvU1DVZ9uJwU_eiuzahfwFXHhCV</recordid><startdate>20170926</startdate><enddate>20170926</enddate><creator>Soria, Ivana</creator><creator>Quattrocchi, Valeria</creator><creator>Langellotti, Cecilia</creator><creator>Gammella, Mariela</creator><creator>Digiacomo, Sebastian</creator><creator>Garcia de la Torre, Beatriz</creator><creator>Andreu, David</creator><creator>Montoya, Maria</creator><creator>Sobrino, Francisco</creator><creator>Blanco, Esther</creator><creator>Zamorano, Patricia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>XX2</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0245-8993</orcidid></search><sort><creationdate>20170926</creationdate><title>Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle</title><author>Soria, Ivana ; Quattrocchi, Valeria ; Langellotti, Cecilia ; Gammella, Mariela ; Digiacomo, Sebastian ; Garcia de la Torre, Beatriz ; Andreu, David ; Montoya, Maria ; Sobrino, Francisco ; Blanco, Esther ; Zamorano, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c734t-af9170340d6fbb9c62215e5c5e10bef283c9082624ed77d9da1d273a49d848113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Biology and Life Sciences</topic><topic>Cattle</topic><topic>Dendrimers</topic><topic>Dendrimers - pharmacology</topic><topic>Epitopes</topic><topic>Foot & mouth disease</topic><topic>Foot and mouth disease</topic><topic>Foot-and-Mouth Disease - prevention & control</topic><topic>Foot-and-Mouth Disease Virus - immunology</topic><topic>Immune response</topic><topic>Immunization</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Immunology</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Medicine and Health Sciences</topic><topic>Mucosa</topic><topic>Peptides</topic><topic>Peptides - pharmacology</topic><topic>Pigs</topic><topic>Prevention</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Secretions</topic><topic>Swine</topic><topic>Vaccines</topic><topic>Viral Vaccines</topic><topic>Viruses</topic><topic>VP1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soria, Ivana</creatorcontrib><creatorcontrib>Quattrocchi, Valeria</creatorcontrib><creatorcontrib>Langellotti, Cecilia</creatorcontrib><creatorcontrib>Gammella, Mariela</creatorcontrib><creatorcontrib>Digiacomo, Sebastian</creatorcontrib><creatorcontrib>Garcia de la Torre, Beatriz</creatorcontrib><creatorcontrib>Andreu, David</creatorcontrib><creatorcontrib>Montoya, Maria</creatorcontrib><creatorcontrib>Sobrino, Francisco</creatorcontrib><creatorcontrib>Blanco, Esther</creatorcontrib><creatorcontrib>Zamorano, Patricia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Recercat</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soria, Ivana</au><au>Quattrocchi, Valeria</au><au>Langellotti, Cecilia</au><au>Gammella, Mariela</au><au>Digiacomo, Sebastian</au><au>Garcia de la Torre, Beatriz</au><au>Andreu, David</au><au>Montoya, Maria</au><au>Sobrino, Francisco</au><au>Blanco, Esther</au><au>Zamorano, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-09-26</date><risdate>2017</risdate><volume>12</volume><issue>9</issue><spage>e0185184</spage><epage>e0185184</epage><pages>e0185184-e0185184</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28949998</pmid><doi>10.1371/journal.pone.0185184</doi><tpages>e0185184</tpages><orcidid>https://orcid.org/0000-0002-0245-8993</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-09, Vol.12 (9), p.e0185184-e0185184 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1943088110 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Recercat; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Antibodies Biology and Life Sciences Cattle Dendrimers Dendrimers - pharmacology Epitopes Foot & mouth disease Foot and mouth disease Foot-and-Mouth Disease - prevention & control Foot-and-Mouth Disease Virus - immunology Immune response Immunization Immunoglobulin A Immunoglobulin G Immunoglobulins Immunology Lymphocytes Lymphocytes B Lymphocytes T Medicine and Health Sciences Mucosa Peptides Peptides - pharmacology Pigs Prevention Proteins Research and Analysis Methods Secretions Swine Vaccines Viral Vaccines Viruses VP1 protein |
| title | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T06%3A05%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dendrimeric%20peptides%20can%20confer%20protection%20against%20foot-and-mouth%20disease%20virus%20in%20cattle&rft.jtitle=PloS%20one&rft.au=Soria,%20Ivana&rft.date=2017-09-26&rft.volume=12&rft.issue=9&rft.spage=e0185184&rft.epage=e0185184&rft.pages=e0185184-e0185184&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0185184&rft_dat=%3Cgale_plos_%3EA506665048%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1943088110&rft_id=info:pmid/28949998&rft_galeid=A506665048&rft_doaj_id=oai_doaj_org_article_799498b29351497bb64c99928e4f1158&rfr_iscdi=true |