Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model
Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models an...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-09, Vol.12 (9), p.e0184557-e0184557 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0184557 |
|---|---|
| container_issue | 9 |
| container_start_page | e0184557 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Rakic Martinez, Mira Wiedmann, Martin Ferguson, Martine Datta, Atin R |
| description | Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study. |
| doi_str_mv | 10.1371/journal.pone.0184557 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1938124743</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A504280648</galeid><doaj_id>oai_doaj_org_article_708bdd25619a4be199320204e986fc69</doaj_id><sourcerecordid>A504280648</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-5c913e1fd4f3e44d81ac17e02bd77dc2578f9c0bb8db3b26a91683be12a387273</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlqm94Iw6LrwMCCX3cS0vR0JkPazCbp4P57U9tdprIXkouG5DnvOedNT5K8xGiJaYE_7G3vOmmWB9vBEmHOsqx4lJzjkpJFThB9fLI_S555v0coozzPnyZnhPOSk5ydJ79W3oP3LXQhtU260T6A0zJtbWfVbbBb6MCnR-16A52CVHdp2EF6JY0ZOTAmFmCMjFceVEiNdEcJUaAG8zx50kjj4cX0vUh-fP70_fLLYnN9tb5cbRYqL0lYZKrEFHBTs4YCYzXHUuECEKnqoqgVyQrelApVFa8rWpFcljjntAJMJOUFKehF8nrUPRjrxeSMF7F_jgkrGI3EeiRqK_fi4HQr3a2wUou_B9ZthXRBKwOiQLyqa5LluJQsJimji4ggBiXPm1hw1Po4ZeurFmoVvXPSzETnN53eia09iiwrM4qHYt5NAs7e9OCDaLVXg4kd2H6sO0eI4aGzN_-gD3c3UVsZG9BdY2NeNYiKVYYY4ShnPFLLB6i4ami1iq_Y6Hg-C3g_C4hMgN9hK3vvxfrb1_9nr3_O2bcn7A6kCTtvTR-07fwcZCOonPXeQXNvMkZimII7N8QwBWKaghj26vSB7oPufnv6B9doAi0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1938124743</pqid></control><display><type>article</type><title>Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Rakic Martinez, Mira ; Wiedmann, Martin ; Ferguson, Martine ; Datta, Atin R</creator><contributor>Ingmer, Hanne</contributor><creatorcontrib>Rakic Martinez, Mira ; Wiedmann, Martin ; Ferguson, Martine ; Datta, Atin R ; Ingmer, Hanne</creatorcontrib><description>Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0184557</identifier><identifier>PMID: 28898264</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal models ; Animals ; Bacteria ; Bacterial infections ; Biology and Life Sciences ; Caenorhabditis elegans ; Clinical isolates ; Deletion mutant ; Disease Models, Animal ; Drosophila melanogaster ; Food ; Food processing industry ; Galleria mellonella ; Gastroenteritis ; Gene Deletion ; Genes ; Genetic aspects ; Inoculation ; Inoculum ; Insects ; Larva - microbiology ; Larvae ; Lepidoptera - growth & development ; Lepidoptera - microbiology ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - genetics ; Listeria monocytogenes - pathogenicity ; Listeriosis ; Listeriosis - microbiology ; Medicine and Health Sciences ; Mutants ; Nutrition ; Outbreaks ; Pathogenicity ; Pathogens ; Pest outbreaks ; Physiological aspects ; Strains (organisms) ; Virulence ; Virulence (Microbiology) ; Virulence - genetics</subject><ispartof>PloS one, 2017-09, Vol.12 (9), p.e0184557-e0184557</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-5c913e1fd4f3e44d81ac17e02bd77dc2578f9c0bb8db3b26a91683be12a387273</citedby><cites>FETCH-LOGICAL-c692t-5c913e1fd4f3e44d81ac17e02bd77dc2578f9c0bb8db3b26a91683be12a387273</cites><orcidid>0000-0002-4168-5662</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595313/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595313/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28898264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ingmer, Hanne</contributor><creatorcontrib>Rakic Martinez, Mira</creatorcontrib><creatorcontrib>Wiedmann, Martin</creatorcontrib><creatorcontrib>Ferguson, Martine</creatorcontrib><creatorcontrib>Datta, Atin R</creatorcontrib><title>Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study.</description><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biology and Life Sciences</subject><subject>Caenorhabditis elegans</subject><subject>Clinical isolates</subject><subject>Deletion mutant</subject><subject>Disease Models, Animal</subject><subject>Drosophila melanogaster</subject><subject>Food</subject><subject>Food processing industry</subject><subject>Galleria mellonella</subject><subject>Gastroenteritis</subject><subject>Gene Deletion</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Inoculation</subject><subject>Inoculum</subject><subject>Insects</subject><subject>Larva - microbiology</subject><subject>Larvae</subject><subject>Lepidoptera - growth & development</subject><subject>Lepidoptera - microbiology</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Listeriosis</subject><subject>Listeriosis - microbiology</subject><subject>Medicine and Health Sciences</subject><subject>Mutants</subject><subject>Nutrition</subject><subject>Outbreaks</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pest outbreaks</subject><subject>Physiological aspects</subject><subject>Strains (organisms)</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Virulence - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlqm94Iw6LrwMCCX3cS0vR0JkPazCbp4P57U9tdprIXkouG5DnvOedNT5K8xGiJaYE_7G3vOmmWB9vBEmHOsqx4lJzjkpJFThB9fLI_S555v0coozzPnyZnhPOSk5ydJ79W3oP3LXQhtU260T6A0zJtbWfVbbBb6MCnR-16A52CVHdp2EF6JY0ZOTAmFmCMjFceVEiNdEcJUaAG8zx50kjj4cX0vUh-fP70_fLLYnN9tb5cbRYqL0lYZKrEFHBTs4YCYzXHUuECEKnqoqgVyQrelApVFa8rWpFcljjntAJMJOUFKehF8nrUPRjrxeSMF7F_jgkrGI3EeiRqK_fi4HQr3a2wUou_B9ZthXRBKwOiQLyqa5LluJQsJimji4ggBiXPm1hw1Po4ZeurFmoVvXPSzETnN53eia09iiwrM4qHYt5NAs7e9OCDaLVXg4kd2H6sO0eI4aGzN_-gD3c3UVsZG9BdY2NeNYiKVYYY4ShnPFLLB6i4ami1iq_Y6Hg-C3g_C4hMgN9hK3vvxfrb1_9nr3_O2bcn7A6kCTtvTR-07fwcZCOonPXeQXNvMkZimII7N8QwBWKaghj26vSB7oPufnv6B9doAi0</recordid><startdate>20170912</startdate><enddate>20170912</enddate><creator>Rakic Martinez, Mira</creator><creator>Wiedmann, Martin</creator><creator>Ferguson, Martine</creator><creator>Datta, Atin R</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4168-5662</orcidid></search><sort><creationdate>20170912</creationdate><title>Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model</title><author>Rakic Martinez, Mira ; Wiedmann, Martin ; Ferguson, Martine ; Datta, Atin R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-5c913e1fd4f3e44d81ac17e02bd77dc2578f9c0bb8db3b26a91683be12a387273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Animal models</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biology and Life Sciences</topic><topic>Caenorhabditis elegans</topic><topic>Clinical isolates</topic><topic>Deletion mutant</topic><topic>Disease Models, Animal</topic><topic>Drosophila melanogaster</topic><topic>Food</topic><topic>Food processing industry</topic><topic>Galleria mellonella</topic><topic>Gastroenteritis</topic><topic>Gene Deletion</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Inoculation</topic><topic>Inoculum</topic><topic>Insects</topic><topic>Larva - microbiology</topic><topic>Larvae</topic><topic>Lepidoptera - growth & development</topic><topic>Lepidoptera - microbiology</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Listeriosis</topic><topic>Listeriosis - microbiology</topic><topic>Medicine and Health Sciences</topic><topic>Mutants</topic><topic>Nutrition</topic><topic>Outbreaks</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Pest outbreaks</topic><topic>Physiological aspects</topic><topic>Strains (organisms)</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rakic Martinez, Mira</creatorcontrib><creatorcontrib>Wiedmann, Martin</creatorcontrib><creatorcontrib>Ferguson, Martine</creatorcontrib><creatorcontrib>Datta, Atin R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rakic Martinez, Mira</au><au>Wiedmann, Martin</au><au>Ferguson, Martine</au><au>Datta, Atin R</au><au>Ingmer, Hanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-09-12</date><risdate>2017</risdate><volume>12</volume><issue>9</issue><spage>e0184557</spage><epage>e0184557</epage><pages>e0184557-e0184557</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28898264</pmid><doi>10.1371/journal.pone.0184557</doi><tpages>e0184557</tpages><orcidid>https://orcid.org/0000-0002-4168-5662</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-09, Vol.12 (9), p.e0184557-e0184557 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1938124743 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal models Animals Bacteria Bacterial infections Biology and Life Sciences Caenorhabditis elegans Clinical isolates Deletion mutant Disease Models, Animal Drosophila melanogaster Food Food processing industry Galleria mellonella Gastroenteritis Gene Deletion Genes Genetic aspects Inoculation Inoculum Insects Larva - microbiology Larvae Lepidoptera - growth & development Lepidoptera - microbiology Listeria Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - pathogenicity Listeriosis Listeriosis - microbiology Medicine and Health Sciences Mutants Nutrition Outbreaks Pathogenicity Pathogens Pest outbreaks Physiological aspects Strains (organisms) Virulence Virulence (Microbiology) Virulence - genetics |
| title | Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A14%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20Listeria%20monocytogenes%20virulence%20in%20the%20Galleria%20mellonella%20insect%20larvae%20model&rft.jtitle=PloS%20one&rft.au=Rakic%20Martinez,%20Mira&rft.date=2017-09-12&rft.volume=12&rft.issue=9&rft.spage=e0184557&rft.epage=e0184557&rft.pages=e0184557-e0184557&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0184557&rft_dat=%3Cgale_plos_%3EA504280648%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1938124743&rft_id=info:pmid/28898264&rft_galeid=A504280648&rft_doaj_id=oai_doaj_org_article_708bdd25619a4be199320204e986fc69&rfr_iscdi=true |