Periplasmic flagella in Borrelia burgdoferi function to maintain cellular integrity upon external stress
Tapping mode atomic force microscopy (AFM) in solution was used to analyze the role of the internally located periplasmic flagella (PFs) of the Lyme disease spirochete Borrelia burgdorferi in withstanding externally applied cellular stresses. By systematically imaging immobilized spirochetes with in...
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| Veröffentlicht in: | PloS one 2017-09, Vol.12 (9), p.e0184648-e0184648 |
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| creator | Kumar, Bharath Miller, Kelly Charon, Nyles W Legleiter, Justin |
| description | Tapping mode atomic force microscopy (AFM) in solution was used to analyze the role of the internally located periplasmic flagella (PFs) of the Lyme disease spirochete Borrelia burgdorferi in withstanding externally applied cellular stresses. By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress. |
| doi_str_mv | 10.1371/journal.pone.0184648 |
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By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0184648</identifier><identifier>PMID: 28898274</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Atomic force microscopy ; Bacteria ; Biology and Life Sciences ; Borrelia ; Borrelia - genetics ; Borrelia - metabolism ; Borrelia - ultrastructure ; Borrelia burgdorferi ; Byrd, Robert C ; Cell size ; Cytoskeleton ; Damage ; Dissipation ; Flagella ; Flagella - genetics ; Flagella - metabolism ; Flagella - ultrastructure ; Gene expression ; Health aspects ; Health sciences ; Immunology ; Integrity ; Lyme disease ; Mammals ; Mechanical properties ; Medicine and Health Sciences ; Microscopy ; Morphology ; Motility ; Movement ; Mutants ; Mutation ; Physical Sciences ; Proteins ; Research and Analysis Methods ; Spirochetes ; Stress, Mechanical ; Stress, Physiological ; Vector-borne diseases</subject><ispartof>PloS one, 2017-09, Vol.12 (9), p.e0184648-e0184648</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Kumar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress.</description><subject>Atomic force microscopy</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Borrelia</subject><subject>Borrelia - genetics</subject><subject>Borrelia - metabolism</subject><subject>Borrelia - ultrastructure</subject><subject>Borrelia burgdorferi</subject><subject>Byrd, Robert C</subject><subject>Cell size</subject><subject>Cytoskeleton</subject><subject>Damage</subject><subject>Dissipation</subject><subject>Flagella</subject><subject>Flagella - genetics</subject><subject>Flagella - metabolism</subject><subject>Flagella - ultrastructure</subject><subject>Gene expression</subject><subject>Health aspects</subject><subject>Health sciences</subject><subject>Immunology</subject><subject>Integrity</subject><subject>Lyme disease</subject><subject>Mammals</subject><subject>Mechanical properties</subject><subject>Medicine and Health Sciences</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Motility</subject><subject>Movement</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Spirochetes</subject><subject>Stress, Mechanical</subject><subject>Stress, Physiological</subject><subject>Vector-borne 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Brian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Periplasmic flagella in Borrelia burgdoferi function to maintain cellular integrity upon external stress</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-09-12</date><risdate>2017</risdate><volume>12</volume><issue>9</issue><spage>e0184648</spage><epage>e0184648</epage><pages>e0184648-e0184648</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tapping mode atomic force microscopy (AFM) in solution was used to analyze the role of the internally located periplasmic flagella (PFs) of the Lyme disease spirochete Borrelia burgdorferi in withstanding externally applied cellular stresses. By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28898274</pmid><doi>10.1371/journal.pone.0184648</doi><tpages>e0184648</tpages><orcidid>https://orcid.org/0000-0002-5262-1867</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Atomic force microscopy Bacteria Biology and Life Sciences Borrelia Borrelia - genetics Borrelia - metabolism Borrelia - ultrastructure Borrelia burgdorferi Byrd, Robert C Cell size Cytoskeleton Damage Dissipation Flagella Flagella - genetics Flagella - metabolism Flagella - ultrastructure Gene expression Health aspects Health sciences Immunology Integrity Lyme disease Mammals Mechanical properties Medicine and Health Sciences Microscopy Morphology Motility Movement Mutants Mutation Physical Sciences Proteins Research and Analysis Methods Spirochetes Stress, Mechanical Stress, Physiological Vector-borne diseases |
| title | Periplasmic flagella in Borrelia burgdoferi function to maintain cellular integrity upon external stress |
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