Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools
Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T....
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| creator | Ng-Nguyen, Dinh Stevenson, Mark A Dorny, Pierre Gabriël, Sarah Vo, Tinh Van Nguyen, Van-Anh Thi Phan, Trong Van Hii, Sze Fui Traub, Rebecca J |
| description | Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T. solium taeniasis carriers. In areas where all three species of Taenia tapeworms (T. solium, Taenia saginata and Taenia asiatica) occur sympatrically, conventional microscope- and copro-antigen based diagnostic methods are unable to distinguish between these three Taenia species. Molecular diagnostic tools have been developed to overcome this limitation; however, conventional PCR-based techniques remain unsuitable for large-scale deployment in community-based surveys. Moreover, a real-time PCR (qPCR) for the discrimination of all three species of Taenia in human stool does not exist. This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools collected from communities in the Central Highlands of Vietnam. The diagnostic characteristics of the test are compared with conventional Kato Katz (KK) thick smear and copro-antigen ELISA (cAgELISA) method utilizing fecal samples from a community based cross-sectional study. Using this new multiplex real-time PCR we provide an estimate of the true prevalence of taeniasis in the source population for the community based cross-sectional study.
Primers and TaqMan probes for the specific amplification of T. solium, T. saginata and T. asiatica were designed and successfully optimized to target the internal transcribed spacer I (ITS-1) gene of T. solium and the cytochrome oxidase subunit I (COX-1) gene of T. saginata and T. asiatica. The newly designed triplex qPCR (T3qPCR) was compared to KK and cAgELISA for the detection of Taenia eggs in stool samples collected from 342 individuals in Dak Lak province, Central Highlands of Vietnam. The overall apparent prevalence of taeniasis in Dak Lak province was 6.72% (95% confidence interval (CI) [3.94-9.50]) in which T. solium accounted for 1.17% (95% CI [0.37-3.17]), according to the T3qPCR. There was sympatric presence of T. solium, T. saginata and T. asiatica. The T3qPCR proved superior to KK and cAgELISA for the detection and differentiation of Taenia species in human feces. Diagnostic sensitivities of 0.94 (95% credible interval (CrI) [0.88-0.98]), 0.82 (95% Cr |
| doi_str_mv | 10.1371/journal.pntd.0005743 |
| format | Article |
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Primers and TaqMan probes for the specific amplification of T. solium, T. saginata and T. asiatica were designed and successfully optimized to target the internal transcribed spacer I (ITS-1) gene of T. solium and the cytochrome oxidase subunit I (COX-1) gene of T. saginata and T. asiatica. The newly designed triplex qPCR (T3qPCR) was compared to KK and cAgELISA for the detection of Taenia eggs in stool samples collected from 342 individuals in Dak Lak province, Central Highlands of Vietnam. The overall apparent prevalence of taeniasis in Dak Lak province was 6.72% (95% confidence interval (CI) [3.94-9.50]) in which T. solium accounted for 1.17% (95% CI [0.37-3.17]), according to the T3qPCR. There was sympatric presence of T. solium, T. saginata and T. asiatica. The T3qPCR proved superior to KK and cAgELISA for the detection and differentiation of Taenia species in human feces. Diagnostic sensitivities of 0.94 (95% credible interval (CrI) [0.88-0.98]), 0.82 (95% CrI [0.58-0.95]) and 0.52 (95% CrI [0.07-0.94]), and diagnostic specificities of 0.98 (95% CrI [0.94-1.00]), 0.91 (95% CrI [0.85-0.96]) and 0.99 (95% CrI [0.96-1.00]) were estimated for the diagnosis of taeniasis for the T3qPCR, cAgELISA and KK thick smear in this study, respectively.
T3qPCR is not only superior to the KK thick smear and cAgELISA in terms of diagnostic sensitivity and specificity, but it also has the advantage of discriminating between species of Taenia eggs in stools. Application of this newly developed T3qPCR has identified the existence of all three human Taenia tapeworms in Dak Lak province and proves for the first time, the existence of T. asiatica in the Central Highlands and the south of Vietnam.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0005743</identifier><identifier>PMID: 28686662</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal sciences ; Animals ; Antigens ; Biology and Life Sciences ; Care and treatment ; Communities ; Comparative analysis ; Confidence intervals ; Countries ; Cross-Sectional Studies ; Cyclooxygenase-1 ; Cysticercosis ; Cytochrome ; Cytochrome oxidase ; Cytochromes ; Deployment ; Detection ; Developing countries ; Diagnostic software ; Diagnostic systems ; Differentiation ; DNA ; DNA Primers ; Economic aspects ; Eggs ; ELISA ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Feces ; Feces - parasitology ; Highlands ; Humans ; Internet ; LDCs ; Markov analysis ; Medicine and Health Sciences ; Multiplex Polymerase Chain Reaction - methods ; Multiplexing ; Neurocysticercosis - diagnosis ; Neurocysticercosis - parasitology ; Nucleotide sequence ; Oxidase ; PCR ; People and Places ; Polymerase chain reaction ; Pork tapeworm ; Primers ; Probes ; Real time ; Real-Time Polymerase Chain Reaction - methods ; Research and Analysis Methods ; Samples ; Sensitivity ; Sensitivity and Specificity ; Smear ; Spacer ; Species ; Specificity ; Surveys ; Sympatric populations ; Taenia saginata - isolation & purification ; Taenia solium - isolation & purification ; Tropical diseases ; Veterinary medicine ; Vietnam ; Worms ; Zoonoses</subject><ispartof>PLoS neglected tropical diseases, 2017-07, Vol.11 (7), p.e0005743-e0005743</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: spp. in human stools. PLoS Negl Trop Dis 11(7): e0005743. https://doi.org/10.1371/journal.pntd.0005743</rights><rights>2017 Ng-Nguyen et al 2017 Ng-Nguyen et al</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: spp. in human stools. PLoS Negl Trop Dis 11(7): e0005743. https://doi.org/10.1371/journal.pntd.0005743</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-c2d03dddfd847f801c7b3625369c391f7c8bdb15d4feeae7ec5bc9897b9dfcb93</citedby><cites>FETCH-LOGICAL-c585t-c2d03dddfd847f801c7b3625369c391f7c8bdb15d4feeae7ec5bc9897b9dfcb93</cites><orcidid>0000-0002-2028-2186</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517074/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517074/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28686662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng-Nguyen, Dinh</creatorcontrib><creatorcontrib>Stevenson, Mark A</creatorcontrib><creatorcontrib>Dorny, Pierre</creatorcontrib><creatorcontrib>Gabriël, Sarah</creatorcontrib><creatorcontrib>Vo, Tinh Van</creatorcontrib><creatorcontrib>Nguyen, Van-Anh Thi</creatorcontrib><creatorcontrib>Phan, Trong Van</creatorcontrib><creatorcontrib>Hii, Sze Fui</creatorcontrib><creatorcontrib>Traub, Rebecca J</creatorcontrib><title>Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T. solium taeniasis carriers. In areas where all three species of Taenia tapeworms (T. solium, Taenia saginata and Taenia asiatica) occur sympatrically, conventional microscope- and copro-antigen based diagnostic methods are unable to distinguish between these three Taenia species. Molecular diagnostic tools have been developed to overcome this limitation; however, conventional PCR-based techniques remain unsuitable for large-scale deployment in community-based surveys. Moreover, a real-time PCR (qPCR) for the discrimination of all three species of Taenia in human stool does not exist. This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools collected from communities in the Central Highlands of Vietnam. The diagnostic characteristics of the test are compared with conventional Kato Katz (KK) thick smear and copro-antigen ELISA (cAgELISA) method utilizing fecal samples from a community based cross-sectional study. Using this new multiplex real-time PCR we provide an estimate of the true prevalence of taeniasis in the source population for the community based cross-sectional study.
Primers and TaqMan probes for the specific amplification of T. solium, T. saginata and T. asiatica were designed and successfully optimized to target the internal transcribed spacer I (ITS-1) gene of T. solium and the cytochrome oxidase subunit I (COX-1) gene of T. saginata and T. asiatica. The newly designed triplex qPCR (T3qPCR) was compared to KK and cAgELISA for the detection of Taenia eggs in stool samples collected from 342 individuals in Dak Lak province, Central Highlands of Vietnam. The overall apparent prevalence of taeniasis in Dak Lak province was 6.72% (95% confidence interval (CI) [3.94-9.50]) in which T. solium accounted for 1.17% (95% CI [0.37-3.17]), according to the T3qPCR. There was sympatric presence of T. solium, T. saginata and T. asiatica. The T3qPCR proved superior to KK and cAgELISA for the detection and differentiation of Taenia species in human feces. Diagnostic sensitivities of 0.94 (95% credible interval (CrI) [0.88-0.98]), 0.82 (95% CrI [0.58-0.95]) and 0.52 (95% CrI [0.07-0.94]), and diagnostic specificities of 0.98 (95% CrI [0.94-1.00]), 0.91 (95% CrI [0.85-0.96]) and 0.99 (95% CrI [0.96-1.00]) were estimated for the diagnosis of taeniasis for the T3qPCR, cAgELISA and KK thick smear in this study, respectively.
T3qPCR is not only superior to the KK thick smear and cAgELISA in terms of diagnostic sensitivity and specificity, but it also has the advantage of discriminating between species of Taenia eggs in stools. Application of this newly developed T3qPCR has identified the existence of all three human Taenia tapeworms in Dak Lak province and proves for the first time, the existence of T. asiatica in the Central Highlands and the south of Vietnam.</description><subject>Animal sciences</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Communities</subject><subject>Comparative analysis</subject><subject>Confidence intervals</subject><subject>Countries</subject><subject>Cross-Sectional Studies</subject><subject>Cyclooxygenase-1</subject><subject>Cysticercosis</subject><subject>Cytochrome</subject><subject>Cytochrome oxidase</subject><subject>Cytochromes</subject><subject>Deployment</subject><subject>Detection</subject><subject>Developing countries</subject><subject>Diagnostic software</subject><subject>Diagnostic systems</subject><subject>Differentiation</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>Economic aspects</subject><subject>Eggs</subject><subject>ELISA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Feces</subject><subject>Feces - parasitology</subject><subject>Highlands</subject><subject>Humans</subject><subject>Internet</subject><subject>LDCs</subject><subject>Markov analysis</subject><subject>Medicine and Health Sciences</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Multiplexing</subject><subject>Neurocysticercosis - diagnosis</subject><subject>Neurocysticercosis - parasitology</subject><subject>Nucleotide sequence</subject><subject>Oxidase</subject><subject>PCR</subject><subject>People and Places</subject><subject>Polymerase chain reaction</subject><subject>Pork tapeworm</subject><subject>Primers</subject><subject>Probes</subject><subject>Real time</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Research and Analysis Methods</subject><subject>Samples</subject><subject>Sensitivity</subject><subject>Sensitivity and Specificity</subject><subject>Smear</subject><subject>Spacer</subject><subject>Species</subject><subject>Specificity</subject><subject>Surveys</subject><subject>Sympatric populations</subject><subject>Taenia saginata - isolation & purification</subject><subject>Taenia solium - isolation & purification</subject><subject>Tropical diseases</subject><subject>Veterinary medicine</subject><subject>Vietnam</subject><subject>Worms</subject><subject>Zoonoses</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkt1u1DAQhSMEoqXwBggsISFudonj2I5vkFarAhWVQFCuLcce77okcbAdCrwQr4mzu61axI1_vznjGZ-ieIrLJSYcv770UxhUtxyHZJZlWVJek3vFMRaELipO6P1b66PiUYyXmRG0wQ-Lo6phDWOsOi7-rH0_quCiH5C3SKEBrlA_dcmNHfxEAVS3SK4H9Gn9GV25tEVpC-iDSn4efued099Q7EEFpAaDtB-DX6ghuQ0M6PT87MsKWR92UQYS6ORyppk0zloIkEm1O8vZLxQMTqE4jkvkBrSdejWgmLzv4uPigVVdhCeH-aT4-vb0Yv1-cf7x3dl6db7QtKFpoStTEmOMNU3NbVNizVvCKkqY0ERgy3XTmhZTU1sABRw0bbVoBG-FsboV5KR4vtcdOx_locdRYlGJmlSsrDJxtieMV5dyDK5X4Zf0ysndgQ8bqUJyugOpRdtyJphigtQ1KVuDCeGUEbCggdKs9eaQbWp7MDp3I6jujujdm8Ft5cb_kJRiXuYPPyleHQSC_z5BTLJ3UUPXqQH8NL8bc8JKzFlGX_yD_r-6A7VRuQA3WJ_z6llUrmohmKirpszUy1vUNpskbaPvpvkj412w3oM6-BgD2JvacClnG18_Qs42lgcb57Bnt_tyE3TtW_IXrvPxvA</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Ng-Nguyen, Dinh</creator><creator>Stevenson, Mark A</creator><creator>Dorny, Pierre</creator><creator>Gabriël, Sarah</creator><creator>Vo, Tinh Van</creator><creator>Nguyen, Van-Anh Thi</creator><creator>Phan, Trong Van</creator><creator>Hii, Sze Fui</creator><creator>Traub, Rebecca J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2028-2186</orcidid></search><sort><creationdate>20170701</creationdate><title>Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools</title><author>Ng-Nguyen, Dinh ; Stevenson, Mark A ; Dorny, Pierre ; Gabriël, Sarah ; Vo, Tinh Van ; Nguyen, Van-Anh Thi ; Phan, Trong Van ; Hii, Sze Fui ; Traub, Rebecca J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-c2d03dddfd847f801c7b3625369c391f7c8bdb15d4feeae7ec5bc9897b9dfcb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal sciences</topic><topic>Animals</topic><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Care and treatment</topic><topic>Communities</topic><topic>Comparative analysis</topic><topic>Confidence intervals</topic><topic>Countries</topic><topic>Cross-Sectional Studies</topic><topic>Cyclooxygenase-1</topic><topic>Cysticercosis</topic><topic>Cytochrome</topic><topic>Cytochrome oxidase</topic><topic>Cytochromes</topic><topic>Deployment</topic><topic>Detection</topic><topic>Developing countries</topic><topic>Diagnostic software</topic><topic>Diagnostic systems</topic><topic>Differentiation</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>Economic aspects</topic><topic>Eggs</topic><topic>ELISA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Feces</topic><topic>Feces - parasitology</topic><topic>Highlands</topic><topic>Humans</topic><topic>Internet</topic><topic>LDCs</topic><topic>Markov analysis</topic><topic>Medicine and Health Sciences</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Multiplexing</topic><topic>Neurocysticercosis - diagnosis</topic><topic>Neurocysticercosis - parasitology</topic><topic>Nucleotide sequence</topic><topic>Oxidase</topic><topic>PCR</topic><topic>People and Places</topic><topic>Polymerase chain reaction</topic><topic>Pork tapeworm</topic><topic>Primers</topic><topic>Probes</topic><topic>Real time</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Research and Analysis Methods</topic><topic>Samples</topic><topic>Sensitivity</topic><topic>Sensitivity and Specificity</topic><topic>Smear</topic><topic>Spacer</topic><topic>Species</topic><topic>Specificity</topic><topic>Surveys</topic><topic>Sympatric populations</topic><topic>Taenia saginata - isolation & purification</topic><topic>Taenia solium - isolation & purification</topic><topic>Tropical diseases</topic><topic>Veterinary medicine</topic><topic>Vietnam</topic><topic>Worms</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng-Nguyen, Dinh</creatorcontrib><creatorcontrib>Stevenson, Mark A</creatorcontrib><creatorcontrib>Dorny, Pierre</creatorcontrib><creatorcontrib>Gabriël, Sarah</creatorcontrib><creatorcontrib>Vo, Tinh Van</creatorcontrib><creatorcontrib>Nguyen, Van-Anh Thi</creatorcontrib><creatorcontrib>Phan, Trong Van</creatorcontrib><creatorcontrib>Hii, Sze Fui</creatorcontrib><creatorcontrib>Traub, Rebecca J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng-Nguyen, Dinh</au><au>Stevenson, Mark A</au><au>Dorny, Pierre</au><au>Gabriël, Sarah</au><au>Vo, Tinh Van</au><au>Nguyen, Van-Anh Thi</au><au>Phan, Trong Van</au><au>Hii, Sze Fui</au><au>Traub, Rebecca J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>11</volume><issue>7</issue><spage>e0005743</spage><epage>e0005743</epage><pages>e0005743-e0005743</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T. solium taeniasis carriers. In areas where all three species of Taenia tapeworms (T. solium, Taenia saginata and Taenia asiatica) occur sympatrically, conventional microscope- and copro-antigen based diagnostic methods are unable to distinguish between these three Taenia species. Molecular diagnostic tools have been developed to overcome this limitation; however, conventional PCR-based techniques remain unsuitable for large-scale deployment in community-based surveys. Moreover, a real-time PCR (qPCR) for the discrimination of all three species of Taenia in human stool does not exist. This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools collected from communities in the Central Highlands of Vietnam. The diagnostic characteristics of the test are compared with conventional Kato Katz (KK) thick smear and copro-antigen ELISA (cAgELISA) method utilizing fecal samples from a community based cross-sectional study. Using this new multiplex real-time PCR we provide an estimate of the true prevalence of taeniasis in the source population for the community based cross-sectional study.
Primers and TaqMan probes for the specific amplification of T. solium, T. saginata and T. asiatica were designed and successfully optimized to target the internal transcribed spacer I (ITS-1) gene of T. solium and the cytochrome oxidase subunit I (COX-1) gene of T. saginata and T. asiatica. The newly designed triplex qPCR (T3qPCR) was compared to KK and cAgELISA for the detection of Taenia eggs in stool samples collected from 342 individuals in Dak Lak province, Central Highlands of Vietnam. The overall apparent prevalence of taeniasis in Dak Lak province was 6.72% (95% confidence interval (CI) [3.94-9.50]) in which T. solium accounted for 1.17% (95% CI [0.37-3.17]), according to the T3qPCR. There was sympatric presence of T. solium, T. saginata and T. asiatica. The T3qPCR proved superior to KK and cAgELISA for the detection and differentiation of Taenia species in human feces. Diagnostic sensitivities of 0.94 (95% credible interval (CrI) [0.88-0.98]), 0.82 (95% CrI [0.58-0.95]) and 0.52 (95% CrI [0.07-0.94]), and diagnostic specificities of 0.98 (95% CrI [0.94-1.00]), 0.91 (95% CrI [0.85-0.96]) and 0.99 (95% CrI [0.96-1.00]) were estimated for the diagnosis of taeniasis for the T3qPCR, cAgELISA and KK thick smear in this study, respectively.
T3qPCR is not only superior to the KK thick smear and cAgELISA in terms of diagnostic sensitivity and specificity, but it also has the advantage of discriminating between species of Taenia eggs in stools. Application of this newly developed T3qPCR has identified the existence of all three human Taenia tapeworms in Dak Lak province and proves for the first time, the existence of T. asiatica in the Central Highlands and the south of Vietnam.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28686662</pmid><doi>10.1371/journal.pntd.0005743</doi><orcidid>https://orcid.org/0000-0002-2028-2186</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2017-07, Vol.11 (7), p.e0005743-e0005743 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_1929432602 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Animal sciences Animals Antigens Biology and Life Sciences Care and treatment Communities Comparative analysis Confidence intervals Countries Cross-Sectional Studies Cyclooxygenase-1 Cysticercosis Cytochrome Cytochrome oxidase Cytochromes Deployment Detection Developing countries Diagnostic software Diagnostic systems Differentiation DNA DNA Primers Economic aspects Eggs ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Feces Feces - parasitology Highlands Humans Internet LDCs Markov analysis Medicine and Health Sciences Multiplex Polymerase Chain Reaction - methods Multiplexing Neurocysticercosis - diagnosis Neurocysticercosis - parasitology Nucleotide sequence Oxidase PCR People and Places Polymerase chain reaction Pork tapeworm Primers Probes Real time Real-Time Polymerase Chain Reaction - methods Research and Analysis Methods Samples Sensitivity Sensitivity and Specificity Smear Spacer Species Specificity Surveys Sympatric populations Taenia saginata - isolation & purification Taenia solium - isolation & purification Tropical diseases Veterinary medicine Vietnam Worms Zoonoses |
| title | Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools |
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