Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Phenotypic assays using human primary cells are highly valuable tools for target discovery and validation in drug discovery. Expression knockdown (KD) of such targets in these assays allows the investigation of their role in models of disease processes. Therefore, efficient and fast modes of protein...

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Veröffentlicht in:	PloS one 2017-08, Vol.12 (8), p.e0182974
Hauptverfasser:	Voets, Olaf, Tielen, Frans, Elstak, Edo, Benschop, Julian, Grimbergen, Max, Stallen, Jan, Janssen, Richard, van Marle, Andre, Essrich, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Actins - genetics Actins - metabolism Adenoviridae - genetics Adenoviridae - metabolism Adenoviruses Assaying Base Sequence Biology and Life Sciences Biotechnology Bronchi - cytology Bronchi - metabolism Cell lines Clonal deletion Clustered Regularly Interspaced Short Palindromic Repeats CRISPR CRISPR-Cas Systems Deactivation Deoxyribonucleic acid DNA Drug discovery Editing Endonucleases - genetics Endonucleases - metabolism Engineering and Technology Epithelial cells Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelial-Mesenchymal Transition - drug effects Epithelial-Mesenchymal Transition - genetics Expression vectors Fibroblasts Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Fibronectin Fibronectins Fibronectins - genetics Fibronectins - metabolism Fibrosis Gene Editing Gene expression Gene Expression Regulation Gene Silencing Genetic engineering Genetic Vectors - chemistry Genetic Vectors - metabolism Genome editing Genome, Human Genomes gRNA Humans Inactivation INDEL Mutation Insertion Lungs Medicine and Health Sciences Mesenchyme Mutagenesis Mutation Nuclear transport Physical Sciences Plasmids Primary Cell Culture Protein Transport Research and Analysis Methods RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism Smad3 protein Smad3 Protein - antagonists & inhibitors Smad3 Protein - genetics Smad3 Protein - metabolism Smooth muscle Stem cells Transduction, Genetic Transforming Growth Factor beta - pharmacology Translocation
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