Identification of a common Ara h 3 epitope recognized by both the capture and the detection monoclonal antibodies in an ELISA detection kit

Allergy to peanuts has become a common and severe problem, especially in westernized countries. In this study, we evaluated the target and epitope specificity of the capture and detection mouse monoclonal antibodies (mAbs) used in a commercial peanut allergen detection platform. We first identified...

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Veröffentlicht in:	PloS one 2017-08, Vol.12 (8), p.e0182935-e0182935
Hauptverfasser:	Zhao, Lipei, Zhao, Liang, Zhang, Buchang, Robotham, Jason M, Roux, Kenneth H, Tang, Hengli
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine Alanine - chemistry Alanine - genetics Alanine - immunology Allergens Allergens - chemistry Allergens - immunology Allergies Amino Acid Sequence Amino Acid Substitution Amino acids Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Antigenic determinants Antigens, Plant - chemistry Antigens, Plant - genetics Antigens, Plant - immunology Ara h 3 antigen Arachis - chemistry Arachis - immunology Binding Biology and Life Sciences Chromatography Cloning Diagnostic systems Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - standards Epitope Mapping Epitopes Epitopes - analysis Epitopes - chemistry Epitopes - immunology Food allergies Gene Expression Health sciences Humans Hypersensitivity Identification and classification Immunoblotting Inhibition Isoelectric focusing Life sciences Liquid chromatography Mass spectrometry Mass spectroscopy Medicine and Health Sciences Mice Models, Molecular Monoclonal antibodies Nuts Peanut Hypersensitivity - diagnosis Peanut Hypersensitivity - immunology Peanuts Peptides Physical Sciences Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - immunology Protein Array Analysis Protein Multimerization Protein Structure, Secondary Proteins Reagent Kits, Diagnostic Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Research and Analysis Methods Seed Storage Proteins - chemistry Seed Storage Proteins - genetics Seed Storage Proteins - immunology Target recognition Testing
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