Two populations of less-virulent Helicobacter pylori genotypes in Bangladesh
Bangladesh has a population with a low gastric cancer risk but high prevalence of Helicobacter pylori infection. Several studies have examined virulence genes in H. pylori from Bangladesh. We analyzed cagA and vacA subtypes and their association with severe histology phenotypes, and analyzed populat...
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| description | Bangladesh has a population with a low gastric cancer risk but high prevalence of Helicobacter pylori infection. Several studies have examined virulence genes in H. pylori from Bangladesh. We analyzed cagA and vacA subtypes and their association with severe histology phenotypes, and analyzed population types among Bangladeshi strains. We included patients who underwent endoscopy in Dhaka. Sequences of virulence genes and seven housekeeping genes were obtained by next generation sequencing and confirmed by Sanger sequencing. We isolated 56 H. pylori strains from 133 patients, of which 73.2% carried cagA, and all were considered Western-type. Patients infected with cagA-positive strains had more severe histological scores than patients infected with cagA-negative strains. Among vacA s1 and m1 genotypes, the s1a (97.8%, 43/44) and m1c (28/30, 93.3%) genotypes were predominant. All strains containing s1 and m1 (30/56, 53.6%) also had i1, d1, and c1. In contrast, all strains containing the less-virulent genotypes s2 and m2 (12/56, 21.4%) also possessed i2, d2, and c2. Multivariate analysis indicated that subjects infected with vacA m1-genotype strains only had a significantly higher risk of antrum atrophy than patients infected with m2-genotype strains. Of the two main H. pylori populations in this study, hpAsia2 strains were associated with higher activity and inflammation in the antrum compared to hpEurope strains; however, only vacA s1m1i1d1c1 strains, independent of population type, were significantly associated with inflammation in the antrum, unlike the s2m2i2d2c2 genotype. In conclusion, Bangladeshi strains were divided into two main populations of different genotypes. The low incidence of gastric cancer in Bangladesh might be attributable to the high proportion of less-virulent genotypes, which may be a better predictor of gastric cancer risk than the ancestral origin of the H. pylori strains. Finally, the vacA m region may be a better virulence marker than other regions. |
| doi_str_mv | 10.1371/journal.pone.0182947 |
| format | Article |
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Several studies have examined virulence genes in H. pylori from Bangladesh. We analyzed cagA and vacA subtypes and their association with severe histology phenotypes, and analyzed population types among Bangladeshi strains. We included patients who underwent endoscopy in Dhaka. Sequences of virulence genes and seven housekeeping genes were obtained by next generation sequencing and confirmed by Sanger sequencing. We isolated 56 H. pylori strains from 133 patients, of which 73.2% carried cagA, and all were considered Western-type. Patients infected with cagA-positive strains had more severe histological scores than patients infected with cagA-negative strains. Among vacA s1 and m1 genotypes, the s1a (97.8%, 43/44) and m1c (28/30, 93.3%) genotypes were predominant. All strains containing s1 and m1 (30/56, 53.6%) also had i1, d1, and c1. In contrast, all strains containing the less-virulent genotypes s2 and m2 (12/56, 21.4%) also possessed i2, d2, and c2. Multivariate analysis indicated that subjects infected with vacA m1-genotype strains only had a significantly higher risk of antrum atrophy than patients infected with m2-genotype strains. Of the two main H. pylori populations in this study, hpAsia2 strains were associated with higher activity and inflammation in the antrum compared to hpEurope strains; however, only vacA s1m1i1d1c1 strains, independent of population type, were significantly associated with inflammation in the antrum, unlike the s2m2i2d2c2 genotype. In conclusion, Bangladeshi strains were divided into two main populations of different genotypes. The low incidence of gastric cancer in Bangladesh might be attributable to the high proportion of less-virulent genotypes, which may be a better predictor of gastric cancer risk than the ancestral origin of the H. pylori strains. Finally, the vacA m region may be a better virulence marker than other regions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0182947</identifier><identifier>PMID: 28797101</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Atrophy ; Bangladesh - epidemiology ; Biology and Life Sciences ; Cancer ; Causes of ; Cytotoxicity ; Disease ; Disease prevention ; Endoscopy ; Female ; Gastric cancer ; Gastroenterology ; Gene sequencing ; Genes ; Genes, Bacterial ; Genetic aspects ; Genotype ; Genotypes ; Health risks ; Helicobacter Infections - complications ; Helicobacter Infections - epidemiology ; Helicobacter Infections - microbiology ; Helicobacter Infections - pathology ; Helicobacter pylori ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Helicobacter pylori - pathogenicity ; Hepatology ; High-Throughput Nucleotide Sequencing ; Histology ; Hospitals ; Humans ; Incidence ; Infections ; M region ; Male ; Medicine and Health Sciences ; Middle Aged ; Multiculturalism & pluralism ; Multivariate analysis ; Patients ; People and Places ; Phylogeny ; Population studies ; Populations ; Preventive medicine ; Research and Analysis Methods ; Risk ; Risk factors ; Signal transduction ; Stomach - microbiology ; Stomach - pathology ; Stomach cancer ; Stomach Neoplasms - epidemiology ; Stomach Neoplasms - microbiology ; Strains (organisms) ; University faculty ; Virulence ; Virulence Factors - genetics ; Young Adult</subject><ispartof>PloS one, 2017-08, Vol.12 (8), p.e0182947-e0182947</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Aftab et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Aftab et al 2017 Aftab et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-e66597a6fb0b36fa176de0c22851232b6fed7e2a400e423d0535f780fc2698903</citedby><cites>FETCH-LOGICAL-c758t-e66597a6fb0b36fa176de0c22851232b6fed7e2a400e423d0535f780fc2698903</cites><orcidid>0000-0002-1222-5819</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552282/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552282/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28797101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Katoh, Masaru</contributor><creatorcontrib>Aftab, Hafeza</creatorcontrib><creatorcontrib>Miftahussurur, Muhammad</creatorcontrib><creatorcontrib>Subsomwong, Phawinee</creatorcontrib><creatorcontrib>Ahmed, Faruque</creatorcontrib><creatorcontrib>Khan, A K Azad</creatorcontrib><creatorcontrib>Matsumoto, Takashi</creatorcontrib><creatorcontrib>Suzuki, Rumiko</creatorcontrib><creatorcontrib>Yamaoka, Yoshio</creatorcontrib><title>Two populations of less-virulent Helicobacter pylori genotypes in Bangladesh</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bangladesh has a population with a low gastric cancer risk but high prevalence of Helicobacter pylori infection. Several studies have examined virulence genes in H. pylori from Bangladesh. We analyzed cagA and vacA subtypes and their association with severe histology phenotypes, and analyzed population types among Bangladeshi strains. We included patients who underwent endoscopy in Dhaka. Sequences of virulence genes and seven housekeeping genes were obtained by next generation sequencing and confirmed by Sanger sequencing. We isolated 56 H. pylori strains from 133 patients, of which 73.2% carried cagA, and all were considered Western-type. Patients infected with cagA-positive strains had more severe histological scores than patients infected with cagA-negative strains. Among vacA s1 and m1 genotypes, the s1a (97.8%, 43/44) and m1c (28/30, 93.3%) genotypes were predominant. All strains containing s1 and m1 (30/56, 53.6%) also had i1, d1, and c1. In contrast, all strains containing the less-virulent genotypes s2 and m2 (12/56, 21.4%) also possessed i2, d2, and c2. Multivariate analysis indicated that subjects infected with vacA m1-genotype strains only had a significantly higher risk of antrum atrophy than patients infected with m2-genotype strains. Of the two main H. pylori populations in this study, hpAsia2 strains were associated with higher activity and inflammation in the antrum compared to hpEurope strains; however, only vacA s1m1i1d1c1 strains, independent of population type, were significantly associated with inflammation in the antrum, unlike the s2m2i2d2c2 genotype. In conclusion, Bangladeshi strains were divided into two main populations of different genotypes. The low incidence of gastric cancer in Bangladesh might be attributable to the high proportion of less-virulent genotypes, which may be a better predictor of gastric cancer risk than the ancestral origin of the H. pylori strains. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aftab, Hafeza</au><au>Miftahussurur, Muhammad</au><au>Subsomwong, Phawinee</au><au>Ahmed, Faruque</au><au>Khan, A K Azad</au><au>Matsumoto, Takashi</au><au>Suzuki, Rumiko</au><au>Yamaoka, Yoshio</au><au>Katoh, Masaru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two populations of less-virulent Helicobacter pylori genotypes in Bangladesh</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-08-10</date><risdate>2017</risdate><volume>12</volume><issue>8</issue><spage>e0182947</spage><epage>e0182947</epage><pages>e0182947-e0182947</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bangladesh has a population with a low gastric cancer risk but high prevalence of Helicobacter pylori infection. Several studies have examined virulence genes in H. pylori from Bangladesh. We analyzed cagA and vacA subtypes and their association with severe histology phenotypes, and analyzed population types among Bangladeshi strains. We included patients who underwent endoscopy in Dhaka. Sequences of virulence genes and seven housekeeping genes were obtained by next generation sequencing and confirmed by Sanger sequencing. We isolated 56 H. pylori strains from 133 patients, of which 73.2% carried cagA, and all were considered Western-type. Patients infected with cagA-positive strains had more severe histological scores than patients infected with cagA-negative strains. Among vacA s1 and m1 genotypes, the s1a (97.8%, 43/44) and m1c (28/30, 93.3%) genotypes were predominant. All strains containing s1 and m1 (30/56, 53.6%) also had i1, d1, and c1. In contrast, all strains containing the less-virulent genotypes s2 and m2 (12/56, 21.4%) also possessed i2, d2, and c2. Multivariate analysis indicated that subjects infected with vacA m1-genotype strains only had a significantly higher risk of antrum atrophy than patients infected with m2-genotype strains. Of the two main H. pylori populations in this study, hpAsia2 strains were associated with higher activity and inflammation in the antrum compared to hpEurope strains; however, only vacA s1m1i1d1c1 strains, independent of population type, were significantly associated with inflammation in the antrum, unlike the s2m2i2d2c2 genotype. In conclusion, Bangladeshi strains were divided into two main populations of different genotypes. The low incidence of gastric cancer in Bangladesh might be attributable to the high proportion of less-virulent genotypes, which may be a better predictor of gastric cancer risk than the ancestral origin of the H. pylori strains. Finally, the vacA m region may be a better virulence marker than other regions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28797101</pmid><doi>10.1371/journal.pone.0182947</doi><tpages>e0182947</tpages><orcidid>https://orcid.org/0000-0002-1222-5819</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-08, Vol.12 (8), p.e0182947-e0182947 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1927795049 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Atrophy Bangladesh - epidemiology Biology and Life Sciences Cancer Causes of Cytotoxicity Disease Disease prevention Endoscopy Female Gastric cancer Gastroenterology Gene sequencing Genes Genes, Bacterial Genetic aspects Genotype Genotypes Health risks Helicobacter Infections - complications Helicobacter Infections - epidemiology Helicobacter Infections - microbiology Helicobacter Infections - pathology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Helicobacter pylori - pathogenicity Hepatology High-Throughput Nucleotide Sequencing Histology Hospitals Humans Incidence Infections M region Male Medicine and Health Sciences Middle Aged Multiculturalism & pluralism Multivariate analysis Patients People and Places Phylogeny Population studies Populations Preventive medicine Research and Analysis Methods Risk Risk factors Signal transduction Stomach - microbiology Stomach - pathology Stomach cancer Stomach Neoplasms - epidemiology Stomach Neoplasms - microbiology Strains (organisms) University faculty Virulence Virulence Factors - genetics Young Adult |
| title | Two populations of less-virulent Helicobacter pylori genotypes in Bangladesh |
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