Total glucosides of paeony inhibits lipopolysaccharide-induced proliferation, migration and invasion in androgen insensitive prostate cancer cells
Previous studies demonstrated that inflammatory microenvironment promoted prostate cancer progression. This study investigated whether total glucosides of paeony (TGP), the active constituents extracted from the root of Paeonia Lactiflora Pall, suppressed lipopolysaccharide (LPS)-stimulated prolifer...
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| Veröffentlicht in: | PloS one 2017-08, Vol.12 (8), p.e0182584-e0182584 |
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| creator | Zhang, Zhi-Hui Xie, Dong-Dong Xu, Shen Xia, Mi-Zhen Zhang, Zhi-Qiang Geng, Hao Chen, Lei Wang, Da-Ming Wei, Wei Yu, De-Xin Xu, De-Xiang |
| description | Previous studies demonstrated that inflammatory microenvironment promoted prostate cancer progression. This study investigated whether total glucosides of paeony (TGP), the active constituents extracted from the root of Paeonia Lactiflora Pall, suppressed lipopolysaccharide (LPS)-stimulated proliferation, migration and invasion in androgen insensitive prostate cancer cells. PC-3 cells were incubated with LPS (2.0 μg/mL) in the absence or presence of TGP (312.5 μg /mL). As expected, cells at S phase and nuclear CyclinD1, the markers of cell proliferation, were increased in LPS-stimulated PC-3 cells. Migration activity, as determined by wound-healing assay and transwell migration assay, and invasion activity, as determined by transwell invasion assay, were elevated in LPS-stimulated PC-3 cells. Interestingly, TGP suppressed LPS-stimulated PC-3 cells proliferation. Moreover, TGP inhibited LPS-stimulated migration and invasion of PC-3 cells. Additional experiment showed that TGP inhibited activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK)/p38 in LPS-stimulated PC-3 cells. Correspondingly, TGP attenuated upregulation of interleukin (IL)-6 and IL-8 in LPS-stimulated PC-3 cells. In addition, TGP inhibited nuclear translocation of signal transducer and activator of transcription 3 (STAT3) in LPS-stimulated PC-3 cells. These results suggest that TGP inhibits inflammation-associated STAT3 activation and proliferation, migration and invasion in androgen insensitive prostate cancer cells. |
| doi_str_mv | 10.1371/journal.pone.0182584 |
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This study investigated whether total glucosides of paeony (TGP), the active constituents extracted from the root of Paeonia Lactiflora Pall, suppressed lipopolysaccharide (LPS)-stimulated proliferation, migration and invasion in androgen insensitive prostate cancer cells. PC-3 cells were incubated with LPS (2.0 μg/mL) in the absence or presence of TGP (312.5 μg /mL). As expected, cells at S phase and nuclear CyclinD1, the markers of cell proliferation, were increased in LPS-stimulated PC-3 cells. Migration activity, as determined by wound-healing assay and transwell migration assay, and invasion activity, as determined by transwell invasion assay, were elevated in LPS-stimulated PC-3 cells. Interestingly, TGP suppressed LPS-stimulated PC-3 cells proliferation. Moreover, TGP inhibited LPS-stimulated migration and invasion of PC-3 cells. Additional experiment showed that TGP inhibited activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK)/p38 in LPS-stimulated PC-3 cells. Correspondingly, TGP attenuated upregulation of interleukin (IL)-6 and IL-8 in LPS-stimulated PC-3 cells. In addition, TGP inhibited nuclear translocation of signal transducer and activator of transcription 3 (STAT3) in LPS-stimulated PC-3 cells. These results suggest that TGP inhibits inflammation-associated STAT3 activation and proliferation, migration and invasion in androgen insensitive prostate cancer cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0182584</identifier><identifier>PMID: 28783760</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Active Transport, Cell Nucleus - drug effects ; Androgens ; Apoptosis ; Apoptosis - drug effects ; Arthritis ; Assaying ; Biology and Life Sciences ; Cancer ; Cancer therapies ; Care and treatment ; Cell adhesion & migration ; Cell culture ; Cell cycle ; Cell Line, Tumor ; Cell migration ; Cell Movement - drug effects ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Cell proliferation ; Cell Proliferation - drug effects ; Chinese medicine ; Development and progression ; Enzyme Activation - drug effects ; Genetic aspects ; Glucosides ; Glucosides - pharmacology ; Healing ; Hospitals ; Humans ; Inflammation ; Interleukin 8 ; Interleukin-6 - metabolism ; Interleukin-8 - metabolism ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Male ; MAP kinase ; Medicine and Health Sciences ; Metastasis ; Neoplasm Invasiveness ; NF-kappa B - metabolism ; NF-κB protein ; Nuclear transport ; p38 Mitogen-Activated Protein Kinases - metabolism ; Paeonia - chemistry ; Pathogenesis ; Physiological aspects ; Plant extracts ; Prostate cancer ; Prostatic Neoplasms, Castration-Resistant - pathology ; Protein kinase ; Public health ; Research and Analysis Methods ; Rodents ; S phase ; Signal Transduction - drug effects ; Stat3 protein ; STAT3 Transcription Factor - metabolism ; Toxicology ; Transcription ; Translocation ; Up-Regulation - drug effects ; Urology ; Wound healing</subject><ispartof>PloS one, 2017-08, Vol.12 (8), p.e0182584-e0182584</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Zhang et al 2017 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-923148362c22f9ba86b0617052ff867f46cd3b1b3f745d78d9fc72c3397e408a3</citedby><cites>FETCH-LOGICAL-c758t-923148362c22f9ba86b0617052ff867f46cd3b1b3f745d78d9fc72c3397e408a3</cites><orcidid>0000-0002-5126-4335</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544245/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544245/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28783760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pizzo, Salvatore V</contributor><creatorcontrib>Zhang, Zhi-Hui</creatorcontrib><creatorcontrib>Xie, Dong-Dong</creatorcontrib><creatorcontrib>Xu, Shen</creatorcontrib><creatorcontrib>Xia, Mi-Zhen</creatorcontrib><creatorcontrib>Zhang, Zhi-Qiang</creatorcontrib><creatorcontrib>Geng, Hao</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Wang, Da-Ming</creatorcontrib><creatorcontrib>Wei, Wei</creatorcontrib><creatorcontrib>Yu, De-Xin</creatorcontrib><creatorcontrib>Xu, De-Xiang</creatorcontrib><title>Total glucosides of paeony inhibits lipopolysaccharide-induced proliferation, migration and invasion in androgen insensitive prostate cancer cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Previous studies demonstrated that inflammatory microenvironment promoted prostate cancer progression. This study investigated whether total glucosides of paeony (TGP), the active constituents extracted from the root of Paeonia Lactiflora Pall, suppressed lipopolysaccharide (LPS)-stimulated proliferation, migration and invasion in androgen insensitive prostate cancer cells. PC-3 cells were incubated with LPS (2.0 μg/mL) in the absence or presence of TGP (312.5 μg /mL). As expected, cells at S phase and nuclear CyclinD1, the markers of cell proliferation, were increased in LPS-stimulated PC-3 cells. Migration activity, as determined by wound-healing assay and transwell migration assay, and invasion activity, as determined by transwell invasion assay, were elevated in LPS-stimulated PC-3 cells. Interestingly, TGP suppressed LPS-stimulated PC-3 cells proliferation. Moreover, TGP inhibited LPS-stimulated migration and invasion of PC-3 cells. Additional experiment showed that TGP inhibited activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK)/p38 in LPS-stimulated PC-3 cells. Correspondingly, TGP attenuated upregulation of interleukin (IL)-6 and IL-8 in LPS-stimulated PC-3 cells. In addition, TGP inhibited nuclear translocation of signal transducer and activator of transcription 3 (STAT3) in LPS-stimulated PC-3 cells. These results suggest that TGP inhibits inflammation-associated STAT3 activation and proliferation, migration and invasion in androgen insensitive prostate cancer cells.</description><subject>Activation</subject><subject>Active Transport, Cell Nucleus - drug effects</subject><subject>Androgens</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Arthritis</subject><subject>Assaying</subject><subject>Biology and Life Sciences</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Chinese medicine</subject><subject>Development and progression</subject><subject>Enzyme Activation - drug effects</subject><subject>Genetic aspects</subject><subject>Glucosides</subject><subject>Glucosides - pharmacology</subject><subject>Healing</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Interleukin 8</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-8 - metabolism</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medicine and Health Sciences</subject><subject>Metastasis</subject><subject>Neoplasm Invasiveness</subject><subject>NF-kappa B - metabolism</subject><subject>NF-κB protein</subject><subject>Nuclear transport</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Paeonia - chemistry</subject><subject>Pathogenesis</subject><subject>Physiological aspects</subject><subject>Plant extracts</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms, Castration-Resistant - pathology</subject><subject>Protein kinase</subject><subject>Public health</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>S phase</subject><subject>Signal Transduction - drug effects</subject><subject>Stat3 protein</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Toxicology</subject><subject>Transcription</subject><subject>Translocation</subject><subject>Up-Regulation - drug effects</subject><subject>Urology</subject><subject>Wound healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-K1DAUxoso7rr6BqIFQRScsWnaJrkRlsU_AwsLunob0vSkkyGTdJN0cV7DJzadmV1mZC-kFz1Jf9-XnNNzsuwlKuYIE_Rx5UZvhZkPzsK8QLSsafUoO0UMl7OmLPDjg_gkexbCqihqTJvmaXZSUkIxaYrT7M-1i8LkvRmlC7qDkDuVDwKc3eTaLnWrY8iNHtzgzCYIKZfCJ2ymbTdK6PLBO6MVeBG1sx_yte53YS5slwxuRZgWerv2rocpDmCDjvoWJnWIIkIuhZXgcwnGhOfZEyVMgBf791n288vn64tvs8urr4uL88uZJDWNM1ZiVFHclLIsFWsFbdqiQaSoS6VoQ1TVyA63qMWKVHVHaMeUJKXEmBGoCirwWfZ65zsYF_i-nIEjVtaMFZjiRCx2ROfEig9er4XfcCc0324433Pho5YGeFMz1AAo1ilWMdYxxighokIUtRIkJK9P-9PGdg2dBBu9MEemx1-sXvLe3fK6rqqyqpPBu72BdzcjhMjXOkwFExbcuL03wQWpcZPQN_-gD2e3p3qREtBWuXSunEz5eUohQYhWiZo_QKWng7WWqfeUTvtHgvdHgsRE-B17MYbAFz--_z979euYfXvALkGYuAzOjFO3hWOw2oEydVfwoO6LjAo-jc5dNfg0Onw_Okn26vAH3YvuZgX_BepfF0I</recordid><startdate>20170804</startdate><enddate>20170804</enddate><creator>Zhang, Zhi-Hui</creator><creator>Xie, Dong-Dong</creator><creator>Xu, Shen</creator><creator>Xia, Mi-Zhen</creator><creator>Zhang, Zhi-Qiang</creator><creator>Geng, Hao</creator><creator>Chen, Lei</creator><creator>Wang, Da-Ming</creator><creator>Wei, Wei</creator><creator>Yu, De-Xin</creator><creator>Xu, De-Xiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5126-4335</orcidid></search><sort><creationdate>20170804</creationdate><title>Total glucosides of paeony inhibits lipopolysaccharide-induced proliferation, migration and invasion in androgen insensitive prostate cancer cells</title><author>Zhang, Zhi-Hui ; Xie, Dong-Dong ; Xu, Shen ; Xia, Mi-Zhen ; Zhang, Zhi-Qiang ; Geng, Hao ; Chen, Lei ; Wang, Da-Ming ; Wei, Wei ; Yu, De-Xin ; Xu, De-Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-923148362c22f9ba86b0617052ff867f46cd3b1b3f745d78d9fc72c3397e408a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Activation</topic><topic>Active Transport, Cell Nucleus - 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metabolism</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>MAP kinase</topic><topic>Medicine and Health Sciences</topic><topic>Metastasis</topic><topic>Neoplasm Invasiveness</topic><topic>NF-kappa B - metabolism</topic><topic>NF-κB protein</topic><topic>Nuclear transport</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Paeonia - chemistry</topic><topic>Pathogenesis</topic><topic>Physiological aspects</topic><topic>Plant extracts</topic><topic>Prostate cancer</topic><topic>Prostatic Neoplasms, Castration-Resistant - pathology</topic><topic>Protein kinase</topic><topic>Public health</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>S phase</topic><topic>Signal Transduction - drug effects</topic><topic>Stat3 protein</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Toxicology</topic><topic>Transcription</topic><topic>Translocation</topic><topic>Up-Regulation - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhi-Hui</au><au>Xie, Dong-Dong</au><au>Xu, Shen</au><au>Xia, Mi-Zhen</au><au>Zhang, Zhi-Qiang</au><au>Geng, Hao</au><au>Chen, Lei</au><au>Wang, Da-Ming</au><au>Wei, Wei</au><au>Yu, De-Xin</au><au>Xu, De-Xiang</au><au>Pizzo, Salvatore V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total glucosides of paeony inhibits lipopolysaccharide-induced proliferation, migration and invasion in androgen insensitive prostate cancer cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-08-04</date><risdate>2017</risdate><volume>12</volume><issue>8</issue><spage>e0182584</spage><epage>e0182584</epage><pages>e0182584-e0182584</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Previous studies demonstrated that inflammatory microenvironment promoted prostate cancer progression. This study investigated whether total glucosides of paeony (TGP), the active constituents extracted from the root of Paeonia Lactiflora Pall, suppressed lipopolysaccharide (LPS)-stimulated proliferation, migration and invasion in androgen insensitive prostate cancer cells. PC-3 cells were incubated with LPS (2.0 μg/mL) in the absence or presence of TGP (312.5 μg /mL). As expected, cells at S phase and nuclear CyclinD1, the markers of cell proliferation, were increased in LPS-stimulated PC-3 cells. Migration activity, as determined by wound-healing assay and transwell migration assay, and invasion activity, as determined by transwell invasion assay, were elevated in LPS-stimulated PC-3 cells. Interestingly, TGP suppressed LPS-stimulated PC-3 cells proliferation. Moreover, TGP inhibited LPS-stimulated migration and invasion of PC-3 cells. Additional experiment showed that TGP inhibited activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK)/p38 in LPS-stimulated PC-3 cells. Correspondingly, TGP attenuated upregulation of interleukin (IL)-6 and IL-8 in LPS-stimulated PC-3 cells. In addition, TGP inhibited nuclear translocation of signal transducer and activator of transcription 3 (STAT3) in LPS-stimulated PC-3 cells. These results suggest that TGP inhibits inflammation-associated STAT3 activation and proliferation, migration and invasion in androgen insensitive prostate cancer cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28783760</pmid><doi>10.1371/journal.pone.0182584</doi><tpages>e0182584</tpages><orcidid>https://orcid.org/0000-0002-5126-4335</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-08, Vol.12 (8), p.e0182584-e0182584 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1925990383 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Activation Active Transport, Cell Nucleus - drug effects Androgens Apoptosis Apoptosis - drug effects Arthritis Assaying Biology and Life Sciences Cancer Cancer therapies Care and treatment Cell adhesion & migration Cell culture Cell cycle Cell Line, Tumor Cell migration Cell Movement - drug effects Cell Nucleus - drug effects Cell Nucleus - metabolism Cell proliferation Cell Proliferation - drug effects Chinese medicine Development and progression Enzyme Activation - drug effects Genetic aspects Glucosides Glucosides - pharmacology Healing Hospitals Humans Inflammation Interleukin 8 Interleukin-6 - metabolism Interleukin-8 - metabolism Lipopolysaccharides Lipopolysaccharides - pharmacology Male MAP kinase Medicine and Health Sciences Metastasis Neoplasm Invasiveness NF-kappa B - metabolism NF-κB protein Nuclear transport p38 Mitogen-Activated Protein Kinases - metabolism Paeonia - chemistry Pathogenesis Physiological aspects Plant extracts Prostate cancer Prostatic Neoplasms, Castration-Resistant - pathology Protein kinase Public health Research and Analysis Methods Rodents S phase Signal Transduction - drug effects Stat3 protein STAT3 Transcription Factor - metabolism Toxicology Transcription Translocation Up-Regulation - drug effects Urology Wound healing |
| title | Total glucosides of paeony inhibits lipopolysaccharide-induced proliferation, migration and invasion in androgen insensitive prostate cancer cells |
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