TRACMIT: An effective pipeline for tracking and analyzing cells on micropatterns through mitosis

The use of micropatterns has transformed investigations of dynamic biological processes by enabling the reproducible analysis of live cells using time-lapse fluorescence microscopy. With micropatterns, thousands of individual cells can be efficiently imaged in parallel, rendering the approach well s...

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Veröffentlicht in:	PloS one 2017-07, Vol.12 (7), p.e0179752-e0179752
Hauptverfasser:	Burri, Olivier, Wolf, Benita, Seitz, Arne, Gönczy, Pierre
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaphase Biological activity Biology and Life Sciences Cell division Cell Tracking - methods Cells (Biology) Data processing Datasets Fluorescence Fluorescence microscopy Freeware HeLa Cells Histones - genetics Histones - metabolism Humans Image analysis Image detection Image processing Life sciences Luminescent Proteins - genetics Luminescent Proteins - metabolism Medical research Metaphase Micropatterning Microscopy Microscopy, Fluorescence - methods Mitosis Red Fluorescent Protein Reproducibility of Results Research and analysis methods Screening projects Software packages Source code Spindle Apparatus Time-Lapse Imaging - methods Tracking
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