OPTiM: Optical projection tomography integrated microscope using open-source hardware and software
We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by sta...
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| Veröffentlicht in: | PloS one 2017-07, Vol.12 (7), p.e0180309-e0180309 |
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| creator | Watson, Thomas Andrews, Natalie Davis, Samuel Bugeon, Laurence Dallman, Margaret D McGinty, James |
| description | We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability. |
| doi_str_mv | 10.1371/journal.pone.0180309 |
| format | Article |
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The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0180309</identifier><identifier>PMID: 28700724</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Algorithms ; Animals ; Biology and Life Sciences ; Cell culture ; Computer programs ; Embryos ; Engineering and Technology ; Gene expression ; Hardware ; Lenses ; Life sciences ; Light ; Light sources ; Medical imaging ; Microscopes ; Microscopy ; Microscopy - methods ; Motor task performance ; Open source software ; Optical tomography ; Physical Sciences ; Physics ; Projection ; Research and Analysis Methods ; Scanning ; Software ; Spatial discrimination ; Spatial resolution ; Tomography ; Tomography, Optical - methods ; Viability ; Zebrafish</subject><ispartof>PloS one, 2017-07, Vol.12 (7), p.e0180309-e0180309</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Watson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Watson et al 2017 Watson et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bb7d3557ff437e62a4701b27286fc8c07ae944fd4bab990edd8fb1ab6ccae1bf3</citedby><cites>FETCH-LOGICAL-c692t-bb7d3557ff437e62a4701b27286fc8c07ae944fd4bab990edd8fb1ab6ccae1bf3</cites><orcidid>0000-0002-3282-494X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507440/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507440/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28700724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Degtyar, Vadim E.</contributor><creatorcontrib>Watson, Thomas</creatorcontrib><creatorcontrib>Andrews, Natalie</creatorcontrib><creatorcontrib>Davis, Samuel</creatorcontrib><creatorcontrib>Bugeon, Laurence</creatorcontrib><creatorcontrib>Dallman, Margaret D</creatorcontrib><creatorcontrib>McGinty, James</creatorcontrib><title>OPTiM: Optical projection tomography integrated microscope using open-source hardware and software</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watson, Thomas</au><au>Andrews, Natalie</au><au>Davis, Samuel</au><au>Bugeon, Laurence</au><au>Dallman, Margaret D</au><au>McGinty, James</au><au>Degtyar, Vadim E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OPTiM: Optical projection tomography integrated microscope using open-source hardware and software</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-07-11</date><risdate>2017</risdate><volume>12</volume><issue>7</issue><spage>e0180309</spage><epage>e0180309</epage><pages>e0180309-e0180309</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28700724</pmid><doi>10.1371/journal.pone.0180309</doi><tpages>e0180309</tpages><orcidid>https://orcid.org/0000-0002-3282-494X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Algorithms Animals Biology and Life Sciences Cell culture Computer programs Embryos Engineering and Technology Gene expression Hardware Lenses Life sciences Light Light sources Medical imaging Microscopes Microscopy Microscopy - methods Motor task performance Open source software Optical tomography Physical Sciences Physics Projection Research and Analysis Methods Scanning Software Spatial discrimination Spatial resolution Tomography Tomography, Optical - methods Viability Zebrafish |
| title | OPTiM: Optical projection tomography integrated microscope using open-source hardware and software |
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