Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury

We have developed a single probe method for detecting microRNA from human serum using single molecule arrays, with sequence specificity down to a single base, and without the use of amplification by polymerases. An abasic peptide nucleic acid (PNA) probe-containing a reactive amine instead of a nucl...

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Veröffentlicht in:	PloS one 2017-07, Vol.12 (7), p.e0179669
Hauptverfasser:	Rissin, David M, López-Longarela, Barbara, Pernagallo, Salvatore, Ilyine, Hugh, Vliegenthart, A D Bastiaan, Dear, James W, Díaz-Mochón, Juan J, Duffy, David C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetaminophen Acetaminophen - toxicity Adult Amplification Arrays Assaying Base Sequence Beads Biology and life sciences Biomarkers Biomarkers - blood Biotin Case-Control Studies Chemical and Drug Induced Liver Injury - blood Chemical and Drug Induced Liver Injury - diagnosis Chemical reactions Correlation analysis Covalence Deoxyribonucleic acid Diagnosis DNA DNA microarrays Drug Overdose - blood Drug Overdose - diagnosis Enzymes Galactosidase Gene expression Genetic aspects Genomics Humans Hybridization Immunoassay Injuries Limit of Detection Liver Liver diseases Measurement Medical research MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - genetics miRNA Molecular Diagnostic Techniques Nucleic Acid Hybridization Oil Patients Peptide nucleic acids Physical Sciences Physiological aspects Polymerase Probe method (forecasting) Research and Analysis Methods Ribonucleic acid RNA Sensitivity and Specificity Streptavidin Toxicity Young Adult β-Galactosidase
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