Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia
Three zygotic developmental stages and two somatic Araucaria angustifolia cell lines with contrasting embryogenic potential were analyzed to identify the carbohydrate-mediated responses associated with embryo formation. Using a comparison between zygotic and somatic embryogenesis systems, the non-st...
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| Veröffentlicht in: | PloS one 2017-07, Vol.12 (7), p.e0180051-e0180051 |
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| creator | Navarro, Bruno V Elbl, Paula De Souza, Amanda P Jardim, Vinicius de Oliveira, Leandro F Macedo, Amanda F Dos Santos, André L W Buckeridge, Marcos S Floh, Eny I S |
| description | Three zygotic developmental stages and two somatic Araucaria angustifolia cell lines with contrasting embryogenic potential were analyzed to identify the carbohydrate-mediated responses associated with embryo formation. Using a comparison between zygotic and somatic embryogenesis systems, the non-structural carbohydrate content, cell wall sugar composition and expression of genes involved in sugar sensing were analyzed, and a network analysis was used to identify coordinated features during embryogenesis. We observed that carbohydrate-mediated responses occur mainly during the early stages of zygotic embryo formation, and that during seed development there are coordinated changes that affect the development of the different structures (embryo and megagametophyte). Furthermore, sucrose and starch accumulation were associated with the responsiveness of the cell lines. This study sheds light on how carbohydrate metabolism is influenced during zygotic and somatic embryogenesis in the endangered conifer species, A. angustifolia. |
| doi_str_mv | 10.1371/journal.pone.0180051 |
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Using a comparison between zygotic and somatic embryogenesis systems, the non-structural carbohydrate content, cell wall sugar composition and expression of genes involved in sugar sensing were analyzed, and a network analysis was used to identify coordinated features during embryogenesis. We observed that carbohydrate-mediated responses occur mainly during the early stages of zygotic embryo formation, and that during seed development there are coordinated changes that affect the development of the different structures (embryo and megagametophyte). Furthermore, sucrose and starch accumulation were associated with the responsiveness of the cell lines. 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Using a comparison between zygotic and somatic embryogenesis systems, the non-structural carbohydrate content, cell wall sugar composition and expression of genes involved in sugar sensing were analyzed, and a network analysis was used to identify coordinated features during embryogenesis. We observed that carbohydrate-mediated responses occur mainly during the early stages of zygotic embryo formation, and that during seed development there are coordinated changes that affect the development of the different structures (embryo and megagametophyte). Furthermore, sucrose and starch accumulation were associated with the responsiveness of the cell lines. This study sheds light on how carbohydrate metabolism is influenced during zygotic and somatic embryogenesis in the endangered conifer species, A. angustifolia.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28678868</pmid><doi>10.1371/journal.pone.0180051</doi><orcidid>https://orcid.org/0000-0001-9289-4328</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Araucariaceae Biological research Biology Biology and Life Sciences Biotechnology Carbohydrate Metabolism Carbohydrates Cell lines Cell walls Coniferophyta - genetics Coniferophyta - growth & development Coniferophyta - metabolism Developmental stages Ecology Embryonic development Embryonic growth stage Embryos Endangered & extinct species Endangered Species Gene expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Kinases Laboratories Metabolism Metabolites Morphology Network analysis Physical Sciences Physiological aspects Physiology Plant sciences Proteins Seeds Seeds - genetics Seeds - growth & development Seeds - metabolism Somatic embryogenesis Starch Sucrose Sugar Transcriptome Trees |
| title | Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia |
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