Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry
Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determ...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-06, Vol.12 (6), p.e0179748-e0179748 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0179748 |
|---|---|
| container_issue | 6 |
| container_start_page | e0179748 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Fontanarosa, Carolina Pane, Francesca Sepe, Nunzio Pinto, Gabriella Trifuoggi, Marco Squillace, Marta Errico, Francesco Usiello, Alessandro Pucci, Piero Amoresano, Angela |
| description | Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free d-Asp, l-Asp and N-methyl d-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing l-Asp, d-Asp and N-methyl d-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75-110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for d-Asp, 0.46 pg/μl for l-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for d-Asp, 1.41 pg/μl for l-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of d-Asp, l-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free d-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses. |
| doi_str_mv | 10.1371/journal.pone.0179748 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1914827652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A497380585</galeid><doaj_id>oai_doaj_org_article_2441101abd444f6bb409db9cdade4fac</doaj_id><sourcerecordid>A497380585</sourcerecordid><originalsourceid>FETCH-LOGICAL-c688t-abc8b24aff12f64e8841be46c04bca77448dca5bef2ffd3bb47fcfd15adaf5473</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEolB4AwSWkBBIZLETJ3FukKqWQ6WWinK4tSbOeNdVEgfbqdiX4hlxutuqi3qBcjH25Jt_7BlPkjxjdMHyir27sJMboFuMdsAFZVVdcXEvecTqPEvLjOb3b633ksfeX1Ba5KIsHyZ7WTQZFfRR8ufrBEMwAYK5RNJiQNebIe7sQKwm2iGSo_TAj2_JyWwIDC35kvYYVusuPUrBj-BiNBIzkN5OHknjIK6D8X5CT5o1USvjoCMeI7pRnkVOpy6YsUNyjqCuvKd2MME6MyxJiAT2pAfviR9RBWdjSrd-kjzQ0Hl8urX7yY-PH74ffk5Pzj4dHx6cpKoUIqTQKNFkHLRmmS45CsFZg7xUlDcKqopz0SooGtSZ1m3eNLzSSresgBZ0wat8P3mx0R076-W21F6ymnGRVWWRReJ4Q7QWLuToTA9uLS0YeeWwbiljYYzqUGacM0YZNC3nXJcxG63bplYttMg1qKj1fpttanpsFQ4hFmxHdPfPYFZyaS9lwWtGuYgCr7cCzv6KVQ-yN15h18GAsSfzuYuc1yWbz_3yH_Tu222pJcQLmEHbmFfNovKA11UuaCGKSC3uoOIXe2dUfJfaRP9OwJudgMgE_B2WMHkvj7-d_z979nOXfXWLXSF0YeVtN83Pyu-CfAMqZ713qG-KzKicx-q6GnIeK7kdqxj2_HaDboKu5yj_C5rJIpI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1914827652</pqid></control><display><type>article</type><title>Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Fontanarosa, Carolina ; Pane, Francesca ; Sepe, Nunzio ; Pinto, Gabriella ; Trifuoggi, Marco ; Squillace, Marta ; Errico, Francesco ; Usiello, Alessandro ; Pucci, Piero ; Amoresano, Angela</creator><creatorcontrib>Fontanarosa, Carolina ; Pane, Francesca ; Sepe, Nunzio ; Pinto, Gabriella ; Trifuoggi, Marco ; Squillace, Marta ; Errico, Francesco ; Usiello, Alessandro ; Pucci, Piero ; Amoresano, Angela</creatorcontrib><description>Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free d-Asp, l-Asp and N-methyl d-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing l-Asp, d-Asp and N-methyl d-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75-110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for d-Asp, 0.46 pg/μl for l-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for d-Asp, 1.41 pg/μl for l-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of d-Asp, l-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free d-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0179748</identifier><identifier>PMID: 28662080</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Analysis ; Analytical chemistry ; Animals ; Aspartic Acid - metabolism ; Biology and Life Sciences ; Biotechnology ; Brain ; Brain - metabolism ; Buffers ; Calibration ; Capillary electrophoresis ; Chromatography ; Chromatography, Liquid ; Enzymes ; Extraction ; Glutamic acid receptors ; Growth hormones ; Ions ; Limit of Detection ; Linearity ; Mammals ; Mass spectrometry ; Mass spectroscopy ; Medicine and Health Sciences ; Metabolites ; Mice ; Monitoring ; N-methyl-D-aspartate ; N-Methyl-D-aspartic acid ; N-Methylaspartate - metabolism ; Neurotransmission ; Physical Sciences ; Physiological aspects ; Physiology ; Reference Standards ; Reproducibility ; Reproducibility of Results ; Research and Analysis Methods ; Scientific imaging ; Separation ; Solvents ; Stereoisomerism ; Tandem Mass Spectrometry - methods ; Tissues ; Water analysis</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0179748-e0179748</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Fontanarosa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Fontanarosa et al 2017 Fontanarosa et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c688t-abc8b24aff12f64e8841be46c04bca77448dca5bef2ffd3bb47fcfd15adaf5473</citedby><cites>FETCH-LOGICAL-c688t-abc8b24aff12f64e8841be46c04bca77448dca5bef2ffd3bb47fcfd15adaf5473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491048/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491048/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28662080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fontanarosa, Carolina</creatorcontrib><creatorcontrib>Pane, Francesca</creatorcontrib><creatorcontrib>Sepe, Nunzio</creatorcontrib><creatorcontrib>Pinto, Gabriella</creatorcontrib><creatorcontrib>Trifuoggi, Marco</creatorcontrib><creatorcontrib>Squillace, Marta</creatorcontrib><creatorcontrib>Errico, Francesco</creatorcontrib><creatorcontrib>Usiello, Alessandro</creatorcontrib><creatorcontrib>Pucci, Piero</creatorcontrib><creatorcontrib>Amoresano, Angela</creatorcontrib><title>Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free d-Asp, l-Asp and N-methyl d-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing l-Asp, d-Asp and N-methyl d-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75-110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for d-Asp, 0.46 pg/μl for l-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for d-Asp, 1.41 pg/μl for l-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of d-Asp, l-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free d-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses.</description><subject>Amino acids</subject><subject>Analysis</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Aspartic Acid - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Buffers</subject><subject>Calibration</subject><subject>Capillary electrophoresis</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Enzymes</subject><subject>Extraction</subject><subject>Glutamic acid receptors</subject><subject>Growth hormones</subject><subject>Ions</subject><subject>Limit of Detection</subject><subject>Linearity</subject><subject>Mammals</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine and Health Sciences</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Monitoring</subject><subject>N-methyl-D-aspartate</subject><subject>N-Methyl-D-aspartic acid</subject><subject>N-Methylaspartate - metabolism</subject><subject>Neurotransmission</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Reference Standards</subject><subject>Reproducibility</subject><subject>Reproducibility of Results</subject><subject>Research and Analysis Methods</subject><subject>Scientific imaging</subject><subject>Separation</subject><subject>Solvents</subject><subject>Stereoisomerism</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tissues</subject><subject>Water analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEolB4AwSWkBBIZLETJ3FukKqWQ6WWinK4tSbOeNdVEgfbqdiX4hlxutuqi3qBcjH25Jt_7BlPkjxjdMHyir27sJMboFuMdsAFZVVdcXEvecTqPEvLjOb3b633ksfeX1Ba5KIsHyZ7WTQZFfRR8ufrBEMwAYK5RNJiQNebIe7sQKwm2iGSo_TAj2_JyWwIDC35kvYYVusuPUrBj-BiNBIzkN5OHknjIK6D8X5CT5o1USvjoCMeI7pRnkVOpy6YsUNyjqCuvKd2MME6MyxJiAT2pAfviR9RBWdjSrd-kjzQ0Hl8urX7yY-PH74ffk5Pzj4dHx6cpKoUIqTQKNFkHLRmmS45CsFZg7xUlDcKqopz0SooGtSZ1m3eNLzSSresgBZ0wat8P3mx0R076-W21F6ymnGRVWWRReJ4Q7QWLuToTA9uLS0YeeWwbiljYYzqUGacM0YZNC3nXJcxG63bplYttMg1qKj1fpttanpsFQ4hFmxHdPfPYFZyaS9lwWtGuYgCr7cCzv6KVQ-yN15h18GAsSfzuYuc1yWbz_3yH_Tu222pJcQLmEHbmFfNovKA11UuaCGKSC3uoOIXe2dUfJfaRP9OwJudgMgE_B2WMHkvj7-d_z979nOXfXWLXSF0YeVtN83Pyu-CfAMqZ713qG-KzKicx-q6GnIeK7kdqxj2_HaDboKu5yj_C5rJIpI</recordid><startdate>20170629</startdate><enddate>20170629</enddate><creator>Fontanarosa, Carolina</creator><creator>Pane, Francesca</creator><creator>Sepe, Nunzio</creator><creator>Pinto, Gabriella</creator><creator>Trifuoggi, Marco</creator><creator>Squillace, Marta</creator><creator>Errico, Francesco</creator><creator>Usiello, Alessandro</creator><creator>Pucci, Piero</creator><creator>Amoresano, Angela</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170629</creationdate><title>Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry</title><author>Fontanarosa, Carolina ; Pane, Francesca ; Sepe, Nunzio ; Pinto, Gabriella ; Trifuoggi, Marco ; Squillace, Marta ; Errico, Francesco ; Usiello, Alessandro ; Pucci, Piero ; Amoresano, Angela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c688t-abc8b24aff12f64e8841be46c04bca77448dca5bef2ffd3bb47fcfd15adaf5473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amino acids</topic><topic>Analysis</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Aspartic Acid - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Buffers</topic><topic>Calibration</topic><topic>Capillary electrophoresis</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Enzymes</topic><topic>Extraction</topic><topic>Glutamic acid receptors</topic><topic>Growth hormones</topic><topic>Ions</topic><topic>Limit of Detection</topic><topic>Linearity</topic><topic>Mammals</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicine and Health Sciences</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Monitoring</topic><topic>N-methyl-D-aspartate</topic><topic>N-Methyl-D-aspartic acid</topic><topic>N-Methylaspartate - metabolism</topic><topic>Neurotransmission</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Reference Standards</topic><topic>Reproducibility</topic><topic>Reproducibility of Results</topic><topic>Research and Analysis Methods</topic><topic>Scientific imaging</topic><topic>Separation</topic><topic>Solvents</topic><topic>Stereoisomerism</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tissues</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fontanarosa, Carolina</creatorcontrib><creatorcontrib>Pane, Francesca</creatorcontrib><creatorcontrib>Sepe, Nunzio</creatorcontrib><creatorcontrib>Pinto, Gabriella</creatorcontrib><creatorcontrib>Trifuoggi, Marco</creatorcontrib><creatorcontrib>Squillace, Marta</creatorcontrib><creatorcontrib>Errico, Francesco</creatorcontrib><creatorcontrib>Usiello, Alessandro</creatorcontrib><creatorcontrib>Pucci, Piero</creatorcontrib><creatorcontrib>Amoresano, Angela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fontanarosa, Carolina</au><au>Pane, Francesca</au><au>Sepe, Nunzio</au><au>Pinto, Gabriella</au><au>Trifuoggi, Marco</au><au>Squillace, Marta</au><au>Errico, Francesco</au><au>Usiello, Alessandro</au><au>Pucci, Piero</au><au>Amoresano, Angela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-06-29</date><risdate>2017</risdate><volume>12</volume><issue>6</issue><spage>e0179748</spage><epage>e0179748</epage><pages>e0179748-e0179748</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free d-Asp, l-Asp and N-methyl d-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing l-Asp, d-Asp and N-methyl d-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75-110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for d-Asp, 0.46 pg/μl for l-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for d-Asp, 1.41 pg/μl for l-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of d-Asp, l-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free d-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28662080</pmid><doi>10.1371/journal.pone.0179748</doi><tpages>e0179748</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-06, Vol.12 (6), p.e0179748-e0179748 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1914827652 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Amino acids Analysis Analytical chemistry Animals Aspartic Acid - metabolism Biology and Life Sciences Biotechnology Brain Brain - metabolism Buffers Calibration Capillary electrophoresis Chromatography Chromatography, Liquid Enzymes Extraction Glutamic acid receptors Growth hormones Ions Limit of Detection Linearity Mammals Mass spectrometry Mass spectroscopy Medicine and Health Sciences Metabolites Mice Monitoring N-methyl-D-aspartate N-Methyl-D-aspartic acid N-Methylaspartate - metabolism Neurotransmission Physical Sciences Physiological aspects Physiology Reference Standards Reproducibility Reproducibility of Results Research and Analysis Methods Scientific imaging Separation Solvents Stereoisomerism Tandem Mass Spectrometry - methods Tissues Water analysis |
| title | Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T22%3A40%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20determination%20of%20free%20D-Asp,%20L-Asp%20and%20N-methyl-D-aspartate%20in%20mouse%20brain%20tissues%20by%20chiral%20separation%20and%20Multiple%20Reaction%20Monitoring%20tandem%20mass%20spectrometry&rft.jtitle=PloS%20one&rft.au=Fontanarosa,%20Carolina&rft.date=2017-06-29&rft.volume=12&rft.issue=6&rft.spage=e0179748&rft.epage=e0179748&rft.pages=e0179748-e0179748&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0179748&rft_dat=%3Cgale_plos_%3EA497380585%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1914827652&rft_id=info:pmid/28662080&rft_galeid=A497380585&rft_doaj_id=oai_doaj_org_article_2441101abd444f6bb409db9cdade4fac&rfr_iscdi=true |