In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives
Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibili...
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| creator | Abriouel, Hikmate Pérez Montoro, Beatriz Casado Muñoz, María Del Carmen Knapp, Charles W Gálvez, Antonio Benomar, Nabil |
| description | Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations. |
| doi_str_mv | 10.1371/journal.pone.0176801 |
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The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0176801</identifier><identifier>PMID: 28651019</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptability ; Adaptation ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Arrays ; Biology and Life Sciences ; Brines ; CRISPR ; CRISPR-Associated Proteins - genetics ; Defense mechanisms ; Drug resistance ; Drug Resistance, Bacterial - genetics ; Ecological monitoring ; Efflux ; Engineering and Technology ; Fermentation ; Fermented food ; Food ; Food Microbiology ; Food Safety ; Fruits ; Genes ; Genome, Bacterial ; Genomes ; Immune system ; Lactobacilli ; Lactobacillus pentosus - genetics ; Lactobacillus pentosus - isolation & purification ; Medicine and Health Sciences ; Niches ; Nucleotide sequence ; Olea - chemistry ; Olives ; Probiotics ; Prophages ; Research and Analysis Methods ; Safety ; Saline water ; Similarity ; Transcription ; Transposons ; Virulence</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0176801-e0176801</ispartof><rights>2017 Abriouel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. 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This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.</description><subject>Adaptability</subject><subject>Adaptation</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Arrays</subject><subject>Biology and Life Sciences</subject><subject>Brines</subject><subject>CRISPR</subject><subject>CRISPR-Associated Proteins - genetics</subject><subject>Defense mechanisms</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Ecological monitoring</subject><subject>Efflux</subject><subject>Engineering and Technology</subject><subject>Fermentation</subject><subject>Fermented food</subject><subject>Food</subject><subject>Food Microbiology</subject><subject>Food Safety</subject><subject>Fruits</subject><subject>Genes</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Immune system</subject><subject>Lactobacilli</subject><subject>Lactobacillus pentosus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abriouel, Hikmate</au><au>Pérez Montoro, Beatriz</au><au>Casado Muñoz, María Del Carmen</au><au>Knapp, Charles W</au><au>Gálvez, Antonio</au><au>Benomar, Nabil</au><au>Lin, Baochuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-06-26</date><risdate>2017</risdate><volume>12</volume><issue>6</issue><spage>e0176801</spage><epage>e0176801</epage><pages>e0176801-e0176801</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28651019</pmid><doi>10.1371/journal.pone.0176801</doi><orcidid>https://orcid.org/0000-0002-0666-3978</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adaptability Adaptation Antibiotic resistance Antibiotics Antimicrobial agents Arrays Biology and Life Sciences Brines CRISPR CRISPR-Associated Proteins - genetics Defense mechanisms Drug resistance Drug Resistance, Bacterial - genetics Ecological monitoring Efflux Engineering and Technology Fermentation Fermented food Food Food Microbiology Food Safety Fruits Genes Genome, Bacterial Genomes Immune system Lactobacilli Lactobacillus pentosus - genetics Lactobacillus pentosus - isolation & purification Medicine and Health Sciences Niches Nucleotide sequence Olea - chemistry Olives Probiotics Prophages Research and Analysis Methods Safety Saline water Similarity Transcription Transposons Virulence |
| title | In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T06%3A22%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20silico%20genomic%20insights%20into%20aspects%20of%20food%20safety%20and%20defense%20mechanisms%20of%20a%20potentially%20probiotic%20Lactobacillus%20pentosus%20MP-10%20isolated%20from%20brines%20of%20naturally%20fermented%20Alore%C3%B1a%20green%20table%20olives&rft.jtitle=PloS%20one&rft.au=Abriouel,%20Hikmate&rft.date=2017-06-26&rft.volume=12&rft.issue=6&rft.spage=e0176801&rft.epage=e0176801&rft.pages=e0176801-e0176801&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0176801&rft_dat=%3Cproquest_plos_%3E1914287187%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1913783732&rft_id=info:pmid/28651019&rft_doaj_id=oai_doaj_org_article_3144a8a9a01f4e978acb7484c512d5ed&rfr_iscdi=true |