Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains
Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 wer...
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| description | Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin. All eleven genes of one bovine RVA strain were closely related to those of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it emerged from multiple reassortment events involving different host species. The porcine RVA strains had mixed genotype backbones with possible multiple reassortant events with strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses found in domestic animals in Uganda strongly suggested the presence of human-to animal RVA transmission, with concomitant circulation of multi-reassortant strains potentially derived from complex interspecies transmission events. However, whole genome data from the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda indicated that they were primarily transmitted from person-to-person. |
| doi_str_mv | 10.1371/journal.pone.0178855 |
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To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin. All eleven genes of one bovine RVA strain were closely related to those of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it emerged from multiple reassortment events involving different host species. The porcine RVA strains had mixed genotype backbones with possible multiple reassortant events with strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses found in domestic animals in Uganda strongly suggested the presence of human-to animal RVA transmission, with concomitant circulation of multi-reassortant strains potentially derived from complex interspecies transmission events. However, whole genome data from the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda indicated that they were primarily transmitted from person-to-person.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0178855</identifier><identifier>PMID: 28640820</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Backbone ; Biology and Life Sciences ; Birds ; Cattle ; Children ; Circulation ; Classification ; Computer and Information Sciences ; Diarrhea ; Disease transmission ; Domestic animals ; Epidemiology ; Gene Rearrangement ; Genes ; Genetics ; Genome, Viral - genetics ; Genomes ; Genomics ; Genotype ; Goats - virology ; Humans ; Immunology ; Infections ; Infectious diseases ; Mammals ; Medicine and Health Sciences ; People and Places ; Phylogeny ; Research and Analysis Methods ; Rotavirus ; Rotavirus - classification ; Rotavirus - genetics ; Rotavirus - isolation & purification ; Rotavirus - physiology ; Species Specificity ; Swine - virology ; Transcription ; Uganda ; Virology ; Viruses ; Zoonoses</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0178855-e0178855</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Bwogi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin. All eleven genes of one bovine RVA strain were closely related to those of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it emerged from multiple reassortment events involving different host species. The porcine RVA strains had mixed genotype backbones with possible multiple reassortant events with strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses found in domestic animals in Uganda strongly suggested the presence of human-to animal RVA transmission, with concomitant circulation of multi-reassortant strains potentially derived from complex interspecies transmission events. However, whole genome data from the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda indicated that they were primarily transmitted from person-to-person.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28640820</pmid><doi>10.1371/journal.pone.0178855</doi><tpages>e0178855</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-06, Vol.12 (6), p.e0178855-e0178855 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1912556199 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; PMC (PubMed Central); Full-Text Journals in Chemistry (Open access); Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Analysis Animals Backbone Biology and Life Sciences Birds Cattle Children Circulation Classification Computer and Information Sciences Diarrhea Disease transmission Domestic animals Epidemiology Gene Rearrangement Genes Genetics Genome, Viral - genetics Genomes Genomics Genotype Goats - virology Humans Immunology Infections Infectious diseases Mammals Medicine and Health Sciences People and Places Phylogeny Research and Analysis Methods Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotavirus - physiology Species Specificity Swine - virology Transcription Uganda Virology Viruses Zoonoses |
| title | Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T17%3A08%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Whole%20genome%20analysis%20of%20selected%20human%20and%20animal%20rotaviruses%20identified%20in%20Uganda%20from%202012%20to%202014%20reveals%20complex%20genome%20reassortment%20events%20between%20human,%20bovine,%20caprine%20and%20porcine%20strains&rft.jtitle=PloS%20one&rft.au=Bwogi,%20Josephine&rft.date=2017-06-22&rft.volume=12&rft.issue=6&rft.spage=e0178855&rft.epage=e0178855&rft.pages=e0178855-e0178855&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0178855&rft_dat=%3Cgale_plos_%3EA543295297%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1912556199&rft_id=info:pmid/28640820&rft_galeid=A543295297&rft_doaj_id=oai_doaj_org_article_4d400c1c36c943f38ac8298c6ac48626&rfr_iscdi=true |