Characterization of slow-cycling cells in the mouse cochlear lateral wall
Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SL...
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| description | Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies. |
| doi_str_mv | 10.1371/journal.pone.0179293 |
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In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0179293</identifier><identifier>PMID: 28632772</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology and Life Sciences ; Biomarkers - metabolism ; Bromodeoxyuridine ; Bromodeoxyuridine - metabolism ; Caspase ; Caspase-3 ; Cell Cycle ; Cell division ; Cell Proliferation ; Cell self-renewal ; Cells, Cultured ; Cochlea ; Cochlea - cytology ; Cochlea - physiology ; Cycles ; Damage ; Deoxyribonucleic acid ; DNA ; Exposure ; Fibroblasts - cytology ; Fibroblasts - physiology ; Genetic aspects ; Hearing - physiology ; Hearing loss ; Histone H3 ; Homeostasis ; Immunohistochemistry ; Laboratory animals ; Li, Yang ; Ligaments ; Male ; Mammals ; Medical research ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Neurosciences ; Noise ; Noise levels ; Otolaryngology ; Physiological aspects ; Regeneration ; Research and Analysis Methods ; Rodents ; Stem cells ; Studies</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0179293-e0179293</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies.</description><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers - metabolism</subject><subject>Bromodeoxyuridine</subject><subject>Bromodeoxyuridine - metabolism</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell Cycle</subject><subject>Cell division</subject><subject>Cell Proliferation</subject><subject>Cell self-renewal</subject><subject>Cells, Cultured</subject><subject>Cochlea</subject><subject>Cochlea - cytology</subject><subject>Cochlea - physiology</subject><subject>Cycles</subject><subject>Damage</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Exposure</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Genetic aspects</subject><subject>Hearing - physiology</subject><subject>Hearing loss</subject><subject>Histone H3</subject><subject>Homeostasis</subject><subject>Immunohistochemistry</subject><subject>Laboratory animals</subject><subject>Li, Yang</subject><subject>Ligaments</subject><subject>Male</subject><subject>Mammals</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neurosciences</subject><subject>Noise</subject><subject>Noise levels</subject><subject>Otolaryngology</subject><subject>Physiological aspects</subject><subject>Regeneration</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Stem 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of slow-cycling cells in the mouse cochlear lateral wall</title><author>Li, Yang ; Watanabe, Kotaro ; Fujioka, Masato ; Ogawa, Kaoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-5f2184a6cf57a4ac71641f4aa989101dc0aef36ecb9cc27db9b6f85aedcba9b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers - metabolism</topic><topic>Bromodeoxyuridine</topic><topic>Bromodeoxyuridine - metabolism</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell Cycle</topic><topic>Cell division</topic><topic>Cell Proliferation</topic><topic>Cell self-renewal</topic><topic>Cells, Cultured</topic><topic>Cochlea</topic><topic>Cochlea - cytology</topic><topic>Cochlea - physiology</topic><topic>Cycles</topic><topic>Damage</topic><topic>Deoxyribonucleic 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Masato</au><au>Ogawa, Kaoru</au><au>Riley, Bruce B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of slow-cycling cells in the mouse cochlear lateral wall</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-06-20</date><risdate>2017</risdate><volume>12</volume><issue>6</issue><spage>e0179293</spage><epage>e0179293</epage><pages>e0179293-e0179293</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28632772</pmid><doi>10.1371/journal.pone.0179293</doi><tpages>e0179293</tpages><orcidid>https://orcid.org/0000-0002-5317-0885</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Biology and Life Sciences Biomarkers - metabolism Bromodeoxyuridine Bromodeoxyuridine - metabolism Caspase Caspase-3 Cell Cycle Cell division Cell Proliferation Cell self-renewal Cells, Cultured Cochlea Cochlea - cytology Cochlea - physiology Cycles Damage Deoxyribonucleic acid DNA Exposure Fibroblasts - cytology Fibroblasts - physiology Genetic aspects Hearing - physiology Hearing loss Histone H3 Homeostasis Immunohistochemistry Laboratory animals Li, Yang Ligaments Male Mammals Medical research Medicine Medicine and Health Sciences Mice Mice, Inbred C57BL Neurosciences Noise Noise levels Otolaryngology Physiological aspects Regeneration Research and Analysis Methods Rodents Stem cells Studies |
| title | Characterization of slow-cycling cells in the mouse cochlear lateral wall |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T15%3A45%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20slow-cycling%20cells%20in%20the%20mouse%20cochlear%20lateral%20wall&rft.jtitle=PloS%20one&rft.au=Li,%20Yang&rft.date=2017-06-20&rft.volume=12&rft.issue=6&rft.spage=e0179293&rft.epage=e0179293&rft.pages=e0179293-e0179293&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0179293&rft_dat=%3Cgale_plos_%3EA496023738%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1911586304&rft_id=info:pmid/28632772&rft_galeid=A496023738&rft_doaj_id=oai_doaj_org_article_0cf443d5c08c469398cad3957996a98f&rfr_iscdi=true |