The infection staging and profile of genotypic distribution and drug resistance mutation among the human immunodeficiency virus-1 infected blood donors from five Chinese blood centers, 2012-2014
The increasing complexity and diversity of the human immunodeficiency virus-1 (HIV-1) infections challenge the disease control and anti-retrovirus treatment in China. The infection stages and molecular characteristics of HIV-1 from infected Chinese blood donors were examined to shed light on the HIV...
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| creator | Zeng, Peibin Liu, Yu He, Miao Wang, Jingxing Keating, Sheila Mao, Wei Huang, Mei Ma, Hongli He, Weilan Bi, Xinhong Liao, Dan Busch, Michael Ness, Paul Liu, Jing Shan, Hua |
| description | The increasing complexity and diversity of the human immunodeficiency virus-1 (HIV-1) infections challenge the disease control and anti-retrovirus treatment in China. The infection stages and molecular characteristics of HIV-1 from infected Chinese blood donors were examined to shed light on the HIV genotype distribution and the status of drug resistance mutations (DRMs) in the changing HIV epidemic in China. Western blot (WB) confirmed HIV-1 positive plasma samples were collected from blood donors at five Chinese blood centers from April 16, 2012, through June 30, 2014. The HIV infection stages were determined using the Lag-avidity assay. HIV Pol regions including whole protease and partial reverse transcriptase (RT) were amplified and sequenced to establish the profile of genotype distribution and drug resistance mutations (DRMs). Viral loads were determined using the ROCHE COBAS system. Of the 259 HIV-1 positive samples tested by the Lag-avidity assay, 23.6% (61/259) were identified as recent infections. A total of 205 amplified sequences displayed the following genotype distributions: circulating recombinant form (CRF) 07_BC (61.5%), CRF08_BC (8.3%), CRF01_AE (20%), B (6.3%), and 01B (3.9%). There was no significant difference in genotype distribution between recent and long-term infections. 31 DRMs were identified from 27 samples including four protease inhibitors (PIs) accessory DRMs, two PIs major DRMs (M46I), two nucleoside RT inhibitors DRMs (K219R and K70Q), and 23 nonnucleoside RT inhibitors DRMs. 27 samples had DRMs, yielding a drug resistance prevalence of 13.2% (27/205). Our findings provide important information for developing strategies for comprehensive HIV control and improving anti-retroviral treatment in China. |
| doi_str_mv | 10.1371/journal.pone.0179328 |
| format | Article |
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The infection stages and molecular characteristics of HIV-1 from infected Chinese blood donors were examined to shed light on the HIV genotype distribution and the status of drug resistance mutations (DRMs) in the changing HIV epidemic in China. Western blot (WB) confirmed HIV-1 positive plasma samples were collected from blood donors at five Chinese blood centers from April 16, 2012, through June 30, 2014. The HIV infection stages were determined using the Lag-avidity assay. HIV Pol regions including whole protease and partial reverse transcriptase (RT) were amplified and sequenced to establish the profile of genotype distribution and drug resistance mutations (DRMs). Viral loads were determined using the ROCHE COBAS system. Of the 259 HIV-1 positive samples tested by the Lag-avidity assay, 23.6% (61/259) were identified as recent infections. A total of 205 amplified sequences displayed the following genotype distributions: circulating recombinant form (CRF) 07_BC (61.5%), CRF08_BC (8.3%), CRF01_AE (20%), B (6.3%), and 01B (3.9%). There was no significant difference in genotype distribution between recent and long-term infections. 31 DRMs were identified from 27 samples including four protease inhibitors (PIs) accessory DRMs, two PIs major DRMs (M46I), two nucleoside RT inhibitors DRMs (K219R and K70Q), and 23 nonnucleoside RT inhibitors DRMs. 27 samples had DRMs, yielding a drug resistance prevalence of 13.2% (27/205). Our findings provide important information for developing strategies for comprehensive HIV control and improving anti-retroviral treatment in China.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0179328</identifier><identifier>PMID: 28622345</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accessories ; Acquired immune deficiency syndrome ; Adolescent ; Adult ; AIDS ; Amplification ; Antimicrobial agents ; Antiretroviral drugs ; Asian People ; Assaying ; Avidity ; Biodiversity ; Biology and Life Sciences ; Blood ; Blood Banks ; Blood Donors ; China - epidemiology ; Complexity ; Disease control ; Disease resistance ; Distribution ; Drug resistance ; Drug Resistance, Viral - genetics ; Epidemics ; Female ; Genetic aspects ; Genotype ; HIV ; HIV Infections - drug therapy ; HIV Infections - epidemiology ; HIV Infections - genetics ; HIV Protease - genetics ; HIV Protease Inhibitors - administration & dosage ; HIV Reverse Transcriptase - genetics ; HIV-1 - genetics ; Human immunodeficiency virus ; Humans ; Infections ; Information dissemination ; Load resistance ; Loads (forces) ; Male ; Medicine and health sciences ; Microbial drug resistance ; Middle Aged ; Mutation ; People and Places ; Protease ; Protease inhibitors ; Proteinase ; Proteinase inhibitors ; Reverse Transcriptase Inhibitors - administration & dosage ; RNA-directed DNA polymerase ; Stress concentration ; Viral Load - methods ; Viruses</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0179328-e0179328</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Zeng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Zeng et al 2017 Zeng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d47f50edfad03537b8a7e75d46a51eb689658690e6e67e39ca60115ee8e64ba03</citedby><cites>FETCH-LOGICAL-c692t-d47f50edfad03537b8a7e75d46a51eb689658690e6e67e39ca60115ee8e64ba03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473534/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473534/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28622345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Peibin</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>He, Miao</creatorcontrib><creatorcontrib>Wang, Jingxing</creatorcontrib><creatorcontrib>Keating, Sheila</creatorcontrib><creatorcontrib>Mao, Wei</creatorcontrib><creatorcontrib>Huang, Mei</creatorcontrib><creatorcontrib>Ma, Hongli</creatorcontrib><creatorcontrib>He, Weilan</creatorcontrib><creatorcontrib>Bi, Xinhong</creatorcontrib><creatorcontrib>Liao, Dan</creatorcontrib><creatorcontrib>Busch, Michael</creatorcontrib><creatorcontrib>Ness, Paul</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Shan, Hua</creatorcontrib><creatorcontrib>NHLBI Recipient Epidemiology and Donor Evaluation Study-III program</creatorcontrib><creatorcontrib>on behalf of the NHLBI Recipient Epidemiology and Donor Evaluation Study-III program</creatorcontrib><title>The infection staging and profile of genotypic distribution and drug resistance mutation among the human immunodeficiency virus-1 infected blood donors from five Chinese blood centers, 2012-2014</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The increasing complexity and diversity of the human immunodeficiency virus-1 (HIV-1) infections challenge the disease control and anti-retrovirus treatment in China. The infection stages and molecular characteristics of HIV-1 from infected Chinese blood donors were examined to shed light on the HIV genotype distribution and the status of drug resistance mutations (DRMs) in the changing HIV epidemic in China. Western blot (WB) confirmed HIV-1 positive plasma samples were collected from blood donors at five Chinese blood centers from April 16, 2012, through June 30, 2014. The HIV infection stages were determined using the Lag-avidity assay. HIV Pol regions including whole protease and partial reverse transcriptase (RT) were amplified and sequenced to establish the profile of genotype distribution and drug resistance mutations (DRMs). Viral loads were determined using the ROCHE COBAS system. Of the 259 HIV-1 positive samples tested by the Lag-avidity assay, 23.6% (61/259) were identified as recent infections. A total of 205 amplified sequences displayed the following genotype distributions: circulating recombinant form (CRF) 07_BC (61.5%), CRF08_BC (8.3%), CRF01_AE (20%), B (6.3%), and 01B (3.9%). There was no significant difference in genotype distribution between recent and long-term infections. 31 DRMs were identified from 27 samples including four protease inhibitors (PIs) accessory DRMs, two PIs major DRMs (M46I), two nucleoside RT inhibitors DRMs (K219R and K70Q), and 23 nonnucleoside RT inhibitors DRMs. 27 samples had DRMs, yielding a drug resistance prevalence of 13.2% (27/205). Our findings provide important information for developing strategies for comprehensive HIV control and improving anti-retroviral treatment in China.</description><subject>Accessories</subject><subject>Acquired immune deficiency syndrome</subject><subject>Adolescent</subject><subject>Adult</subject><subject>AIDS</subject><subject>Amplification</subject><subject>Antimicrobial agents</subject><subject>Antiretroviral drugs</subject><subject>Asian People</subject><subject>Assaying</subject><subject>Avidity</subject><subject>Biodiversity</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood Banks</subject><subject>Blood Donors</subject><subject>China - epidemiology</subject><subject>Complexity</subject><subject>Disease control</subject><subject>Disease resistance</subject><subject>Distribution</subject><subject>Drug resistance</subject><subject>Drug Resistance, Viral - genetics</subject><subject>Epidemics</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>HIV</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - epidemiology</subject><subject>HIV Infections - genetics</subject><subject>HIV Protease - genetics</subject><subject>HIV Protease Inhibitors - administration & dosage</subject><subject>HIV Reverse Transcriptase - genetics</subject><subject>HIV-1 - genetics</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Information dissemination</subject><subject>Load resistance</subject><subject>Loads (forces)</subject><subject>Male</subject><subject>Medicine and health sciences</subject><subject>Microbial drug resistance</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>People and Places</subject><subject>Protease</subject><subject>Protease inhibitors</subject><subject>Proteinase</subject><subject>Proteinase inhibitors</subject><subject>Reverse Transcriptase Inhibitors - administration & dosage</subject><subject>RNA-directed DNA polymerase</subject><subject>Stress concentration</subject><subject>Viral Load - methods</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk21rFDEQxxdRbK1-A9GAIAremWweNvtGKIcPhUJBq29DLjvZS9lNrslusV_PT2a2ty096QtZ2ITMb_6TmcwUxUuCl4RW5ONFGKPX3XIbPCwxqWpaykfFIcnrQpSYPr63PyiepXSBMadSiKfFQSlFWVLGD4s_5xtAzlswgwsepUG3zrdI-wZtY7CuAxQsasGH4XrrDGpcGqJbjzf0RDVxbFGElM-1N4D6cdA7Yx-y0JDlN2OvPXJ9P_rQgHXGgTfX6MrFMS3IHB0atO5CyILBh5iQjaFH1l0BWm2chwSz2YAfIKYPqMSkXOQfe148sbpL8GJej4qfXz6fr74tTs--nqyOTxdG1OWwaFhlOYbG6gZTTqu11BVUvGFCcwJrIWvBpagxCBAV0NpogQnhABIEW2tMj4rXO91tF5Kay58UqQlmEnNWZeJkRzRBX6htdL2O1ypop24OQmyVjoMzHSjDpLSyYpQzyyita10aa2sKWlotS561Ps3RxnUPzZR21N2e6L7Fu41qw5WaLsIpywLvZoEYLkdIg-pdMtB12kMYd_euallLkdE3_6APZzdTrc4J5FcLOa6ZRNUxq3mFBeYyU8sHqPw10DuTe3XqqX2H93sOmRng99DqMSV18uP7_7Nnv_bZt_fYDehu2KTQ3XRu2gfZDjQxpBTB3hWZYDWN2m011DRqah617Pbq_gPdOd3OFv0L7wkmlg</recordid><startdate>20170616</startdate><enddate>20170616</enddate><creator>Zeng, Peibin</creator><creator>Liu, Yu</creator><creator>He, Miao</creator><creator>Wang, Jingxing</creator><creator>Keating, Sheila</creator><creator>Mao, Wei</creator><creator>Huang, Mei</creator><creator>Ma, Hongli</creator><creator>He, Weilan</creator><creator>Bi, Xinhong</creator><creator>Liao, Dan</creator><creator>Busch, Michael</creator><creator>Ness, Paul</creator><creator>Liu, Jing</creator><creator>Shan, Hua</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170616</creationdate><title>The infection staging and profile of genotypic distribution and drug resistance mutation among the human immunodeficiency virus-1 infected blood donors from five Chinese blood centers, 2012-2014</title><author>Zeng, Peibin ; Liu, Yu ; He, Miao ; Wang, Jingxing ; Keating, Sheila ; Mao, Wei ; Huang, Mei ; Ma, Hongli ; He, Weilan ; Bi, Xinhong ; Liao, Dan ; Busch, Michael ; Ness, Paul ; Liu, Jing ; Shan, Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d47f50edfad03537b8a7e75d46a51eb689658690e6e67e39ca60115ee8e64ba03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Accessories</topic><topic>Acquired immune deficiency syndrome</topic><topic>Adolescent</topic><topic>Adult</topic><topic>AIDS</topic><topic>Amplification</topic><topic>Antimicrobial agents</topic><topic>Antiretroviral drugs</topic><topic>Asian People</topic><topic>Assaying</topic><topic>Avidity</topic><topic>Biodiversity</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood Banks</topic><topic>Blood Donors</topic><topic>China - epidemiology</topic><topic>Complexity</topic><topic>Disease control</topic><topic>Disease resistance</topic><topic>Distribution</topic><topic>Drug resistance</topic><topic>Drug Resistance, Viral - genetics</topic><topic>Epidemics</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>HIV</topic><topic>HIV Infections - drug therapy</topic><topic>HIV Infections - epidemiology</topic><topic>HIV Infections - genetics</topic><topic>HIV Protease - genetics</topic><topic>HIV Protease Inhibitors - administration & dosage</topic><topic>HIV Reverse Transcriptase - genetics</topic><topic>HIV-1 - genetics</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Infections</topic><topic>Information dissemination</topic><topic>Load resistance</topic><topic>Loads (forces)</topic><topic>Male</topic><topic>Medicine and health sciences</topic><topic>Microbial drug resistance</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>People and Places</topic><topic>Protease</topic><topic>Protease inhibitors</topic><topic>Proteinase</topic><topic>Proteinase inhibitors</topic><topic>Reverse Transcriptase Inhibitors - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Peibin</au><au>Liu, Yu</au><au>He, Miao</au><au>Wang, Jingxing</au><au>Keating, Sheila</au><au>Mao, Wei</au><au>Huang, Mei</au><au>Ma, Hongli</au><au>He, Weilan</au><au>Bi, Xinhong</au><au>Liao, Dan</au><au>Busch, Michael</au><au>Ness, Paul</au><au>Liu, Jing</au><au>Shan, Hua</au><aucorp>NHLBI Recipient Epidemiology and Donor Evaluation Study-III program</aucorp><aucorp>on behalf of the NHLBI Recipient Epidemiology and Donor Evaluation Study-III program</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The infection staging and profile of genotypic distribution and drug resistance mutation among the human immunodeficiency virus-1 infected blood donors from five Chinese blood centers, 2012-2014</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-06-16</date><risdate>2017</risdate><volume>12</volume><issue>6</issue><spage>e0179328</spage><epage>e0179328</epage><pages>e0179328-e0179328</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The increasing complexity and diversity of the human immunodeficiency virus-1 (HIV-1) infections challenge the disease control and anti-retrovirus treatment in China. The infection stages and molecular characteristics of HIV-1 from infected Chinese blood donors were examined to shed light on the HIV genotype distribution and the status of drug resistance mutations (DRMs) in the changing HIV epidemic in China. Western blot (WB) confirmed HIV-1 positive plasma samples were collected from blood donors at five Chinese blood centers from April 16, 2012, through June 30, 2014. The HIV infection stages were determined using the Lag-avidity assay. HIV Pol regions including whole protease and partial reverse transcriptase (RT) were amplified and sequenced to establish the profile of genotype distribution and drug resistance mutations (DRMs). Viral loads were determined using the ROCHE COBAS system. Of the 259 HIV-1 positive samples tested by the Lag-avidity assay, 23.6% (61/259) were identified as recent infections. A total of 205 amplified sequences displayed the following genotype distributions: circulating recombinant form (CRF) 07_BC (61.5%), CRF08_BC (8.3%), CRF01_AE (20%), B (6.3%), and 01B (3.9%). There was no significant difference in genotype distribution between recent and long-term infections. 31 DRMs were identified from 27 samples including four protease inhibitors (PIs) accessory DRMs, two PIs major DRMs (M46I), two nucleoside RT inhibitors DRMs (K219R and K70Q), and 23 nonnucleoside RT inhibitors DRMs. 27 samples had DRMs, yielding a drug resistance prevalence of 13.2% (27/205). Our findings provide important information for developing strategies for comprehensive HIV control and improving anti-retroviral treatment in China.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28622345</pmid><doi>10.1371/journal.pone.0179328</doi><tpages>e0179328</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-06, Vol.12 (6), p.e0179328-e0179328 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1910480547 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Accessories Acquired immune deficiency syndrome Adolescent Adult AIDS Amplification Antimicrobial agents Antiretroviral drugs Asian People Assaying Avidity Biodiversity Biology and Life Sciences Blood Blood Banks Blood Donors China - epidemiology Complexity Disease control Disease resistance Distribution Drug resistance Drug Resistance, Viral - genetics Epidemics Female Genetic aspects Genotype HIV HIV Infections - drug therapy HIV Infections - epidemiology HIV Infections - genetics HIV Protease - genetics HIV Protease Inhibitors - administration & dosage HIV Reverse Transcriptase - genetics HIV-1 - genetics Human immunodeficiency virus Humans Infections Information dissemination Load resistance Loads (forces) Male Medicine and health sciences Microbial drug resistance Middle Aged Mutation People and Places Protease Protease inhibitors Proteinase Proteinase inhibitors Reverse Transcriptase Inhibitors - administration & dosage RNA-directed DNA polymerase Stress concentration Viral Load - methods Viruses |
| title | The infection staging and profile of genotypic distribution and drug resistance mutation among the human immunodeficiency virus-1 infected blood donors from five Chinese blood centers, 2012-2014 |
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