Highly dynamic temporal changes of TSPY gene copy number in aging bulls
The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudie...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-05, Vol.12 (5), p.e0178558-e0178558 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0178558 |
|---|---|
| container_issue | 5 |
| container_start_page | e0178558 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Oluwole, Olutobi A Mahboubi, Kiana Favetta, Laura A Revay, Tamas Kroetsch, Tom King, William Allan |
| description | The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudied aspect of the biology of TSPY that has been shown to vary among cattle breeds, individual bulls and somatic tissues. The subjects of this prospective 30-month long study were 25 Holstein bulls, sampled every six months. Real-time quantitative PCR was used to determine the relative TSPY copy number (rTSPY CN) and telomere length in the DNA samples extracted from blood. Twenty bulls showed an altered rTSPY CN after 30 months, although only 9 bulls showed a significant change (4 significant increase while 5 significant decrease, P |
| doi_str_mv | 10.1371/journal.pone.0178558 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1902658310</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A492947211</galeid><doaj_id>oai_doaj_org_article_964c63ebf1904f7290f7390f28859316</doaj_id><sourcerecordid>A492947211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-aa834d403476ebd75e4113734deeacddd70e469ab575bfd036e2ebbfbf2c4e503</originalsourceid><addsrcrecordid>eNqNkl9r1EAUxYMotla_gWhAEH3Ydf5lknkRStF2oVCxVfBpmExusrNMZtJMIu63d7ablo30QQJJuPmdc3PvnCR5jdES0xx_2vixd8ouO-9giXBeZFnxJDnGgpIFJ4g-PXg_Sl6EsEEoowXnz5MjEmEi8uI4Ob8wzdpu02rrVGt0OkDb-V7ZVK-VayCkvk5vrr_9ShtwkGrfbVM3tiX0qXGpaoxr0nK0NrxMntXKBng1PU-SH1-_3JxdLC6vzldnp5cLzQUZFkoVlFUMUZZzKKs8A4bjNLEGoHRVVTkCxoUqszwr6wpRDgTKsi5rohlkiJ4kb_e-nfVBTjsIEgtEeFZQvCNWe6LyaiO73rSq30qvjLwr-L6Rqh-MtiAFZ5pTKOsoZ3VOBKpzGm-kKDJBMY9en6duY9lCpcENcTcz0_kXZ9ay8b9lxhjHHEeDD5NB729HCINsTdBgrXLgx7v_powKgfOIvvsHfXy6iWpUHMC42se-emcqT5kgguUE79ouH6HiVUE85BiY2sT6TPBxJojMAH-GRo0hyNX19_9nr37O2fcH7BqUHdbB23Ew3oU5yPag7n0IPdQPS8ZI7vJ-vw25y7uc8h5lbw4P6EF0H3D6Fywn-S0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1902658310</pqid></control><display><type>article</type><title>Highly dynamic temporal changes of TSPY gene copy number in aging bulls</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Oluwole, Olutobi A ; Mahboubi, Kiana ; Favetta, Laura A ; Revay, Tamas ; Kroetsch, Tom ; King, William Allan</creator><contributor>Paiva, Samuel Rezende</contributor><creatorcontrib>Oluwole, Olutobi A ; Mahboubi, Kiana ; Favetta, Laura A ; Revay, Tamas ; Kroetsch, Tom ; King, William Allan ; Paiva, Samuel Rezende</creatorcontrib><description>The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudied aspect of the biology of TSPY that has been shown to vary among cattle breeds, individual bulls and somatic tissues. The subjects of this prospective 30-month long study were 25 Holstein bulls, sampled every six months. Real-time quantitative PCR was used to determine the relative TSPY copy number (rTSPY CN) and telomere length in the DNA samples extracted from blood. Twenty bulls showed an altered rTSPY CN after 30 months, although only 9 bulls showed a significant change (4 significant increase while 5 significant decrease, P<0.01). The sequential sampling provided the flow of rTSPY CN over six observations in 30 months and wide-spread variation of rTSPY CN was detected. Although a clear trend of the direction of change was not identifiable, the highly dynamic changes of individual rTSPY CN in aging bulls were observed here for the first time. In summary we have observed a highly variable rTSPY CN in bulls over a short period of time. Our results suggest the importance of further long term studies of the dynamics of rTSPY CN variablility.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0178558</identifier><identifier>PMID: 28552978</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Age factors ; Aging ; Aging (artificial) ; Aging (materials) ; Aging - genetics ; Animal health ; Animals ; Artificial insemination ; Biology and Life Sciences ; Blood ; Blood cells ; Brain ; Bulls (Cattle) ; Cancer ; Cattle ; Cell Cycle Proteins - genetics ; Cell division ; Cell survival ; Chloroform ; Chromosome rearrangements ; Chromosomes ; Clusters ; Comminution ; Copy number ; Coronary artery disease ; Denaturation ; Deoxyribonucleic acid ; DNA ; DNA repair ; Elongation ; Embryos ; Fertility ; Food ; Functions (mathematics) ; Gene Dosage ; Gene families ; Genes ; Genetic aspects ; Genetic variation ; Genomes ; Genomic instability ; Heart ; Heart diseases ; Horses ; Housing ; Inspection ; Male ; Mammals ; Mathematical analysis ; Melting ; Mice ; Mortality ; Normality ; Nucleic acids ; Offspring ; Physiological aspects ; Population studies ; Precipitation ; Primates ; Proteins ; Quality ; Rats ; Recombination ; Reproduction ; Research and Analysis Methods ; Samples ; Sampling ; Sperm ; Spermatogenesis ; Statistical analysis ; Statistics ; Stem cells ; Studies</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0178558-e0178558</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Oluwole et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Oluwole et al 2017 Oluwole et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-aa834d403476ebd75e4113734deeacddd70e469ab575bfd036e2ebbfbf2c4e503</citedby><cites>FETCH-LOGICAL-c692t-aa834d403476ebd75e4113734deeacddd70e469ab575bfd036e2ebbfbf2c4e503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446161/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446161/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28552978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Paiva, Samuel Rezende</contributor><creatorcontrib>Oluwole, Olutobi A</creatorcontrib><creatorcontrib>Mahboubi, Kiana</creatorcontrib><creatorcontrib>Favetta, Laura A</creatorcontrib><creatorcontrib>Revay, Tamas</creatorcontrib><creatorcontrib>Kroetsch, Tom</creatorcontrib><creatorcontrib>King, William Allan</creatorcontrib><title>Highly dynamic temporal changes of TSPY gene copy number in aging bulls</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudied aspect of the biology of TSPY that has been shown to vary among cattle breeds, individual bulls and somatic tissues. The subjects of this prospective 30-month long study were 25 Holstein bulls, sampled every six months. Real-time quantitative PCR was used to determine the relative TSPY copy number (rTSPY CN) and telomere length in the DNA samples extracted from blood. Twenty bulls showed an altered rTSPY CN after 30 months, although only 9 bulls showed a significant change (4 significant increase while 5 significant decrease, P<0.01). The sequential sampling provided the flow of rTSPY CN over six observations in 30 months and wide-spread variation of rTSPY CN was detected. Although a clear trend of the direction of change was not identifiable, the highly dynamic changes of individual rTSPY CN in aging bulls were observed here for the first time. In summary we have observed a highly variable rTSPY CN in bulls over a short period of time. Our results suggest the importance of further long term studies of the dynamics of rTSPY CN variablility.</description><subject>Age</subject><subject>Age factors</subject><subject>Aging</subject><subject>Aging (artificial)</subject><subject>Aging (materials)</subject><subject>Aging - genetics</subject><subject>Animal health</subject><subject>Animals</subject><subject>Artificial insemination</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood cells</subject><subject>Brain</subject><subject>Bulls (Cattle)</subject><subject>Cancer</subject><subject>Cattle</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell division</subject><subject>Cell survival</subject><subject>Chloroform</subject><subject>Chromosome rearrangements</subject><subject>Chromosomes</subject><subject>Clusters</subject><subject>Comminution</subject><subject>Copy number</subject><subject>Coronary artery disease</subject><subject>Denaturation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA repair</subject><subject>Elongation</subject><subject>Embryos</subject><subject>Fertility</subject><subject>Food</subject><subject>Functions (mathematics)</subject><subject>Gene Dosage</subject><subject>Gene families</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic variation</subject><subject>Genomes</subject><subject>Genomic instability</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Horses</subject><subject>Housing</subject><subject>Inspection</subject><subject>Male</subject><subject>Mammals</subject><subject>Mathematical analysis</subject><subject>Melting</subject><subject>Mice</subject><subject>Mortality</subject><subject>Normality</subject><subject>Nucleic acids</subject><subject>Offspring</subject><subject>Physiological aspects</subject><subject>Population studies</subject><subject>Precipitation</subject><subject>Primates</subject><subject>Proteins</subject><subject>Quality</subject><subject>Rats</subject><subject>Recombination</subject><subject>Reproduction</subject><subject>Research and Analysis Methods</subject><subject>Samples</subject><subject>Sampling</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>Statistical analysis</subject><subject>Statistics</subject><subject>Stem cells</subject><subject>Studies</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl9r1EAUxYMotla_gWhAEH3Ydf5lknkRStF2oVCxVfBpmExusrNMZtJMIu63d7ablo30QQJJuPmdc3PvnCR5jdES0xx_2vixd8ouO-9giXBeZFnxJDnGgpIFJ4g-PXg_Sl6EsEEoowXnz5MjEmEi8uI4Ob8wzdpu02rrVGt0OkDb-V7ZVK-VayCkvk5vrr_9ShtwkGrfbVM3tiX0qXGpaoxr0nK0NrxMntXKBng1PU-SH1-_3JxdLC6vzldnp5cLzQUZFkoVlFUMUZZzKKs8A4bjNLEGoHRVVTkCxoUqszwr6wpRDgTKsi5rohlkiJ4kb_e-nfVBTjsIEgtEeFZQvCNWe6LyaiO73rSq30qvjLwr-L6Rqh-MtiAFZ5pTKOsoZ3VOBKpzGm-kKDJBMY9en6duY9lCpcENcTcz0_kXZ9ay8b9lxhjHHEeDD5NB729HCINsTdBgrXLgx7v_powKgfOIvvsHfXy6iWpUHMC42se-emcqT5kgguUE79ouH6HiVUE85BiY2sT6TPBxJojMAH-GRo0hyNX19_9nr37O2fcH7BqUHdbB23Ew3oU5yPag7n0IPdQPS8ZI7vJ-vw25y7uc8h5lbw4P6EF0H3D6Fywn-S0</recordid><startdate>20170526</startdate><enddate>20170526</enddate><creator>Oluwole, Olutobi A</creator><creator>Mahboubi, Kiana</creator><creator>Favetta, Laura A</creator><creator>Revay, Tamas</creator><creator>Kroetsch, Tom</creator><creator>King, William Allan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170526</creationdate><title>Highly dynamic temporal changes of TSPY gene copy number in aging bulls</title><author>Oluwole, Olutobi A ; Mahboubi, Kiana ; Favetta, Laura A ; Revay, Tamas ; Kroetsch, Tom ; King, William Allan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-aa834d403476ebd75e4113734deeacddd70e469ab575bfd036e2ebbfbf2c4e503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Age</topic><topic>Age factors</topic><topic>Aging</topic><topic>Aging (artificial)</topic><topic>Aging (materials)</topic><topic>Aging - genetics</topic><topic>Animal health</topic><topic>Animals</topic><topic>Artificial insemination</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood cells</topic><topic>Brain</topic><topic>Bulls (Cattle)</topic><topic>Cancer</topic><topic>Cattle</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell division</topic><topic>Cell survival</topic><topic>Chloroform</topic><topic>Chromosome rearrangements</topic><topic>Chromosomes</topic><topic>Clusters</topic><topic>Comminution</topic><topic>Copy number</topic><topic>Coronary artery disease</topic><topic>Denaturation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA repair</topic><topic>Elongation</topic><topic>Embryos</topic><topic>Fertility</topic><topic>Food</topic><topic>Functions (mathematics)</topic><topic>Gene Dosage</topic><topic>Gene families</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic variation</topic><topic>Genomes</topic><topic>Genomic instability</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Horses</topic><topic>Housing</topic><topic>Inspection</topic><topic>Male</topic><topic>Mammals</topic><topic>Mathematical analysis</topic><topic>Melting</topic><topic>Mice</topic><topic>Mortality</topic><topic>Normality</topic><topic>Nucleic acids</topic><topic>Offspring</topic><topic>Physiological aspects</topic><topic>Population studies</topic><topic>Precipitation</topic><topic>Primates</topic><topic>Proteins</topic><topic>Quality</topic><topic>Rats</topic><topic>Recombination</topic><topic>Reproduction</topic><topic>Research and Analysis Methods</topic><topic>Samples</topic><topic>Sampling</topic><topic>Sperm</topic><topic>Spermatogenesis</topic><topic>Statistical analysis</topic><topic>Statistics</topic><topic>Stem cells</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oluwole, Olutobi A</creatorcontrib><creatorcontrib>Mahboubi, Kiana</creatorcontrib><creatorcontrib>Favetta, Laura A</creatorcontrib><creatorcontrib>Revay, Tamas</creatorcontrib><creatorcontrib>Kroetsch, Tom</creatorcontrib><creatorcontrib>King, William Allan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oluwole, Olutobi A</au><au>Mahboubi, Kiana</au><au>Favetta, Laura A</au><au>Revay, Tamas</au><au>Kroetsch, Tom</au><au>King, William Allan</au><au>Paiva, Samuel Rezende</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly dynamic temporal changes of TSPY gene copy number in aging bulls</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-05-26</date><risdate>2017</risdate><volume>12</volume><issue>5</issue><spage>e0178558</spage><epage>e0178558</epage><pages>e0178558-e0178558</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudied aspect of the biology of TSPY that has been shown to vary among cattle breeds, individual bulls and somatic tissues. The subjects of this prospective 30-month long study were 25 Holstein bulls, sampled every six months. Real-time quantitative PCR was used to determine the relative TSPY copy number (rTSPY CN) and telomere length in the DNA samples extracted from blood. Twenty bulls showed an altered rTSPY CN after 30 months, although only 9 bulls showed a significant change (4 significant increase while 5 significant decrease, P<0.01). The sequential sampling provided the flow of rTSPY CN over six observations in 30 months and wide-spread variation of rTSPY CN was detected. Although a clear trend of the direction of change was not identifiable, the highly dynamic changes of individual rTSPY CN in aging bulls were observed here for the first time. In summary we have observed a highly variable rTSPY CN in bulls over a short period of time. Our results suggest the importance of further long term studies of the dynamics of rTSPY CN variablility.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28552978</pmid><doi>10.1371/journal.pone.0178558</doi><tpages>e0178558</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-05, Vol.12 (5), p.e0178558-e0178558 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1902658310 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age Age factors Aging Aging (artificial) Aging (materials) Aging - genetics Animal health Animals Artificial insemination Biology and Life Sciences Blood Blood cells Brain Bulls (Cattle) Cancer Cattle Cell Cycle Proteins - genetics Cell division Cell survival Chloroform Chromosome rearrangements Chromosomes Clusters Comminution Copy number Coronary artery disease Denaturation Deoxyribonucleic acid DNA DNA repair Elongation Embryos Fertility Food Functions (mathematics) Gene Dosage Gene families Genes Genetic aspects Genetic variation Genomes Genomic instability Heart Heart diseases Horses Housing Inspection Male Mammals Mathematical analysis Melting Mice Mortality Normality Nucleic acids Offspring Physiological aspects Population studies Precipitation Primates Proteins Quality Rats Recombination Reproduction Research and Analysis Methods Samples Sampling Sperm Spermatogenesis Statistical analysis Statistics Stem cells Studies |
| title | Highly dynamic temporal changes of TSPY gene copy number in aging bulls |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T15%3A06%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Highly%20dynamic%20temporal%20changes%20of%20TSPY%20gene%20copy%20number%20in%20aging%20bulls&rft.jtitle=PloS%20one&rft.au=Oluwole,%20Olutobi%20A&rft.date=2017-05-26&rft.volume=12&rft.issue=5&rft.spage=e0178558&rft.epage=e0178558&rft.pages=e0178558-e0178558&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0178558&rft_dat=%3Cgale_plos_%3EA492947211%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1902658310&rft_id=info:pmid/28552978&rft_galeid=A492947211&rft_doaj_id=oai_doaj_org_article_964c63ebf1904f7290f7390f28859316&rfr_iscdi=true |