Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea
Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate...
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| description | Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). |
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This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD).</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0178226</identifier><identifier>PMID: 28542554</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Absorption ; Acetylcholine receptors (muscarinic) ; Acetyltransferase ; Activation ; Aeration ; Aerosols ; Allosteric properties ; Animals ; Anions ; Asthma ; Attenuation ; Bacteria ; Biology and Life Sciences ; Bronchitis ; Cages ; Calcium ; Calcium - metabolism ; Calcium chloride ; Canning ; Carbon dioxide ; Cells, Cultured ; Centrifugation ; Chambers ; Chlorides - metabolism ; Cholinergic transmission ; Chronic obstructive pulmonary disease ; Cloning ; Conductance ; Cystic fibrosis ; Defensive behavior ; Dehydration ; Dilution ; Epithelium ; Epithelium - drug effects ; Epithelium - growth & development ; Epithelium - metabolism ; Female ; Glands ; Glucose ; Groundwater flow ; Heart diseases ; Homeostasis ; Hydration ; Hypersensitivity ; Hypertension ; Hypoxia ; Immunoglobulin G ; Infections ; Inhalation ; Inhibition ; Ion channels ; Ion transport ; Ion Transport - drug effects ; Keratin ; Life sciences ; Lung diseases ; Male ; Medicinal plants ; Medicine and Health Sciences ; Mice ; Modulation ; Mutation ; Nitric oxide ; Pathogens ; Pharmacology ; Phenanthrenes - pharmacology ; Physical Sciences ; Physiology ; Respiratory tract diseases ; Smooth muscle ; Solubility ; Temperature effects ; Trachea ; Trachea - drug effects ; Trachea - growth & development ; Trachea - metabolism ; Vasoactive agents</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0178226-e0178226</ispartof><rights>2017 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Chen et al 2017 Chen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-8fdce899532c0e3d3fa89a839707f6c702fe328b687589465b6a934c04f33adc3</citedby><cites>FETCH-LOGICAL-c526t-8fdce899532c0e3d3fa89a839707f6c702fe328b687589465b6a934c04f33adc3</cites><orcidid>0000-0002-6311-1299</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440052/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440052/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28542554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fehrenbach, Heinz</contributor><creatorcontrib>Chen, Peng-Xiao</creatorcontrib><creatorcontrib>Zhang, Yi-Lin</creatorcontrib><creatorcontrib>Xu, Jia-Wen</creatorcontrib><creatorcontrib>Yu, Ming-Hao</creatorcontrib><creatorcontrib>Huang, Jie-Hong</creatorcontrib><creatorcontrib>Zhao, Lei</creatorcontrib><creatorcontrib>Zhou, Wen-Liang</creatorcontrib><title>Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD).</description><subject>Absorption</subject><subject>Acetylcholine receptors (muscarinic)</subject><subject>Acetyltransferase</subject><subject>Activation</subject><subject>Aeration</subject><subject>Aerosols</subject><subject>Allosteric properties</subject><subject>Animals</subject><subject>Anions</subject><subject>Asthma</subject><subject>Attenuation</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Bronchitis</subject><subject>Cages</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium chloride</subject><subject>Canning</subject><subject>Carbon dioxide</subject><subject>Cells, Cultured</subject><subject>Centrifugation</subject><subject>Chambers</subject><subject>Chlorides - metabolism</subject><subject>Cholinergic transmission</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Cloning</subject><subject>Conductance</subject><subject>Cystic fibrosis</subject><subject>Defensive behavior</subject><subject>Dehydration</subject><subject>Dilution</subject><subject>Epithelium</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - growth & development</subject><subject>Epithelium - metabolism</subject><subject>Female</subject><subject>Glands</subject><subject>Glucose</subject><subject>Groundwater flow</subject><subject>Heart diseases</subject><subject>Homeostasis</subject><subject>Hydration</subject><subject>Hypersensitivity</subject><subject>Hypertension</subject><subject>Hypoxia</subject><subject>Immunoglobulin G</subject><subject>Infections</subject><subject>Inhalation</subject><subject>Inhibition</subject><subject>Ion channels</subject><subject>Ion transport</subject><subject>Ion Transport - drug effects</subject><subject>Keratin</subject><subject>Life sciences</subject><subject>Lung diseases</subject><subject>Male</subject><subject>Medicinal plants</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Modulation</subject><subject>Mutation</subject><subject>Nitric oxide</subject><subject>Pathogens</subject><subject>Pharmacology</subject><subject>Phenanthrenes - pharmacology</subject><subject>Physical Sciences</subject><subject>Physiology</subject><subject>Respiratory tract diseases</subject><subject>Smooth muscle</subject><subject>Solubility</subject><subject>Temperature effects</subject><subject>Trachea</subject><subject>Trachea - drug effects</subject><subject>Trachea - growth & development</subject><subject>Trachea - metabolism</subject><subject>Vasoactive agents</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUktv1DAYjBCIlsI_QBCJSy9Z_H5ckKoVhUiVOABny-vYXa8ce7GTSvx7HDatWsTJn-z5xjOjaZq3EGwg5vDjIc056rA5pmg3AHKBEHvWnEOJUccQwM8fzWfNq1IOAFAsGHvZnCFBCaKUnDf99zT4eWwnHcvex8rV9v1VW-bgUtSTbcvkxznUaWi3oWuLNdlOPsXWx3ZMc7HtlLXZW_26eeF0KPbNel40P68__9h-7W6-fem3VzedoYhNnXCDsUJKipEBFg_YaSG1wJID7pjhADmLkdgxwamQhNEd0xITA4jDWA8GXzTvT7zHkIpaUygKSgAx5IjJiuhPiCHpgzpmP-r8WyXt1d-LlG-VzpM3wSouCTZkgBw6SQbgJKspCmcl5YQRhirXp_W3eTfaKj1Wu-EJ6dOX6PfqNt0pSkjNeyG4XAly-jXbMqnRF2ND0NHW-BbdGDII-KL7wz_Q_7sjJ5TJqZRs3YMYCNTSjPsttTRDrc2oa-8eG3lYuq8C_gMVT7Vz</recordid><startdate>20170522</startdate><enddate>20170522</enddate><creator>Chen, Peng-Xiao</creator><creator>Zhang, Yi-Lin</creator><creator>Xu, Jia-Wen</creator><creator>Yu, Ming-Hao</creator><creator>Huang, Jie-Hong</creator><creator>Zhao, Lei</creator><creator>Zhou, Wen-Liang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6311-1299</orcidid></search><sort><creationdate>20170522</creationdate><title>Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea</title><author>Chen, Peng-Xiao ; Zhang, Yi-Lin ; Xu, Jia-Wen ; Yu, Ming-Hao ; Huang, Jie-Hong ; Zhao, Lei ; Zhou, Wen-Liang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-8fdce899532c0e3d3fa89a839707f6c702fe328b687589465b6a934c04f33adc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Absorption</topic><topic>Acetylcholine receptors (muscarinic)</topic><topic>Acetyltransferase</topic><topic>Activation</topic><topic>Aeration</topic><topic>Aerosols</topic><topic>Allosteric properties</topic><topic>Animals</topic><topic>Anions</topic><topic>Asthma</topic><topic>Attenuation</topic><topic>Bacteria</topic><topic>Biology and Life Sciences</topic><topic>Bronchitis</topic><topic>Cages</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium chloride</topic><topic>Canning</topic><topic>Carbon dioxide</topic><topic>Cells, Cultured</topic><topic>Centrifugation</topic><topic>Chambers</topic><topic>Chlorides - metabolism</topic><topic>Cholinergic transmission</topic><topic>Chronic obstructive pulmonary disease</topic><topic>Cloning</topic><topic>Conductance</topic><topic>Cystic fibrosis</topic><topic>Defensive behavior</topic><topic>Dehydration</topic><topic>Dilution</topic><topic>Epithelium</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - growth & development</topic><topic>Epithelium - metabolism</topic><topic>Female</topic><topic>Glands</topic><topic>Glucose</topic><topic>Groundwater flow</topic><topic>Heart diseases</topic><topic>Homeostasis</topic><topic>Hydration</topic><topic>Hypersensitivity</topic><topic>Hypertension</topic><topic>Hypoxia</topic><topic>Immunoglobulin G</topic><topic>Infections</topic><topic>Inhalation</topic><topic>Inhibition</topic><topic>Ion channels</topic><topic>Ion transport</topic><topic>Ion Transport - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Peng-Xiao</au><au>Zhang, Yi-Lin</au><au>Xu, Jia-Wen</au><au>Yu, Ming-Hao</au><au>Huang, Jie-Hong</au><au>Zhao, Lei</au><au>Zhou, Wen-Liang</au><au>Fehrenbach, Heinz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-05-22</date><risdate>2017</risdate><volume>12</volume><issue>5</issue><spage>e0178226</spage><epage>e0178226</epage><pages>e0178226-e0178226</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD).</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28542554</pmid><doi>10.1371/journal.pone.0178226</doi><orcidid>https://orcid.org/0000-0002-6311-1299</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Absorption Acetylcholine receptors (muscarinic) Acetyltransferase Activation Aeration Aerosols Allosteric properties Animals Anions Asthma Attenuation Bacteria Biology and Life Sciences Bronchitis Cages Calcium Calcium - metabolism Calcium chloride Canning Carbon dioxide Cells, Cultured Centrifugation Chambers Chlorides - metabolism Cholinergic transmission Chronic obstructive pulmonary disease Cloning Conductance Cystic fibrosis Defensive behavior Dehydration Dilution Epithelium Epithelium - drug effects Epithelium - growth & development Epithelium - metabolism Female Glands Glucose Groundwater flow Heart diseases Homeostasis Hydration Hypersensitivity Hypertension Hypoxia Immunoglobulin G Infections Inhalation Inhibition Ion channels Ion transport Ion Transport - drug effects Keratin Life sciences Lung diseases Male Medicinal plants Medicine and Health Sciences Mice Modulation Mutation Nitric oxide Pathogens Pharmacology Phenanthrenes - pharmacology Physical Sciences Physiology Respiratory tract diseases Smooth muscle Solubility Temperature effects Trachea Trachea - drug effects Trachea - growth & development Trachea - metabolism Vasoactive agents |
| title | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A16%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sodium%20tanshinone%20IIA%20sulfonate%20stimulated%20Cl-%20secretion%20in%20mouse%20trachea&rft.jtitle=PloS%20one&rft.au=Chen,%20Peng-Xiao&rft.date=2017-05-22&rft.volume=12&rft.issue=5&rft.spage=e0178226&rft.epage=e0178226&rft.pages=e0178226-e0178226&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0178226&rft_dat=%3Cproquest_plos_%3E1903161079%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1901317269&rft_id=info:pmid/28542554&rft_doaj_id=oai_doaj_org_article_7943c4d171f94d0f962268fe95746462&rfr_iscdi=true |