Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we a...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-05, Vol.12 (5), p.e0177106-e0177106 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0177106 |
|---|---|
| container_issue | 5 |
| container_start_page | e0177106 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Kobatake, Eiji Nakagawa, Hisako Seki, Takahiro Miyazaki, Tadaaki |
| description | Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells. |
| doi_str_mv | 10.1371/journal.pone.0177106 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1898010312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A491534912</galeid><doaj_id>oai_doaj_org_article_1ed0195f29cd4f8e8a66c87f1714223a</doaj_id><sourcerecordid>A491534912</sourcerecordid><originalsourceid>FETCH-LOGICAL-c736t-84c99a8b025e546356041164623750374bd7eb9e81db88535f29de82a1da3fab3</originalsourceid><addsrcrecordid>eNqNk22L1DAQx4so3nn6DUQLguiLXfPQpukb4Tx8WFg48U7fhmmadLO0zZqkx_ntTXd7x1buhQSSMPnNf5LJTJK8xGiJaYE_bO3gemiXO9urJcJFgRF7lJzikpIFI4g-PtqfJM-83yKUU87Y0-SE8KykJSlOE_3d2aBkMDcqVVrHnU-hr1M99NFoY4C0U3IDvfGdT61O1yCDrUCath182oD3ypn06tM1QXmeQgOm9yG1t6aGvagPTnn_PHmiofXqxbSeJT-_fL6--LZYX35dXZyvF7KgLCx4JssSeIVIrvKM0ZyhDGOWMUKLHNEiq-pCVaXiuK44z2muSVkrTgDXQDVU9Cx5fdDdtdaLKUVeYF5yhBHFJBKrA1Fb2IqdMx24P8KCEXuDdY0AF4xslcCqRrgcY8g601xxYEzyQuMCZ4RQiFofp2hD1alaqj44aGei85PebERjb0SeEcbyIgq8mwSc_T0oH0RnvFRtC72yw_7eJUYRHO_95h_04ddNVAPxAabXNsaVo6g4z0qc0ziN1PIBKo5adUbGetIm2mcO72cOkQnqNjQweC9WVz_-n738NWffHrEbBW3YeNsOY-X5OZgdQOms907p-yRjJMZ2uMuGGNtBTO0Q3V4df9C9013907_T4wQ6</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1898010312</pqid></control><display><type>article</type><title>Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kobatake, Eiji ; Nakagawa, Hisako ; Seki, Takahiro ; Miyazaki, Tadaaki</creator><contributor>Pizzo, Salvatore V</contributor><creatorcontrib>Kobatake, Eiji ; Nakagawa, Hisako ; Seki, Takahiro ; Miyazaki, Tadaaki ; Pizzo, Salvatore V</creatorcontrib><description>Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0177106</identifier><identifier>PMID: 28493927</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adipose tissue ; Aging ; Aging (artificial) ; Allergic diseases ; Animals ; Antioxidant Response Elements ; Antioxidants ; Arteriosclerosis ; Assaying ; Atherosclerosis ; Augmentation ; Bacteria ; Biochemistry ; Biology and Life Sciences ; Biotechnology ; Cadmium ; Cancer ; Carbon dioxide ; Cardiovascular diseases ; Catalase ; Cell growth ; Cell Proliferation ; Cells, Cultured ; Cellular signal transduction ; Centrifugation ; Chemokines ; Chemotherapy ; Computer programs ; Cytotoxicity ; Diet ; Disease ; Distilled water ; Embryos ; Enzymes ; Feeding ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Fibroblasts - microbiology ; Fluorescence ; Food ; Gene expression ; Glucose ; Heart ; Heart diseases ; Immunoglobulin A ; Immunology ; Ingredients ; Inhibition ; Intestine ; Isoforms ; JNK Mitogen-Activated Protein Kinases - metabolism ; JNK protein ; Kinases ; Lactobacillus ; Lactobacillus gasseri - metabolism ; Life span ; Liver diseases ; MAP Kinase Signaling System ; Medicine ; Mice ; Microorganisms ; Milk ; NF-E2-Related Factor 2 - genetics ; NF-E2-Related Factor 2 - metabolism ; NIH 3T3 Cells ; Ostomy ; Oxidative Stress ; Oxygen ; Paraquat ; Pharmacology ; Phosphates ; Physiological aspects ; Probiotics ; Probiotics - metabolism ; Protein kinase ; Reactive oxygen species ; Research and Analysis Methods ; Rodents ; Senescence ; Signal Transduction ; Snow ; Toxicity ; Transcription factors ; Viruses</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0177106-e0177106</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Kobatake et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Kobatake et al 2017 Kobatake et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c736t-84c99a8b025e546356041164623750374bd7eb9e81db88535f29de82a1da3fab3</citedby><cites>FETCH-LOGICAL-c736t-84c99a8b025e546356041164623750374bd7eb9e81db88535f29de82a1da3fab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426657/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426657/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23870,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28493927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pizzo, Salvatore V</contributor><creatorcontrib>Kobatake, Eiji</creatorcontrib><creatorcontrib>Nakagawa, Hisako</creatorcontrib><creatorcontrib>Seki, Takahiro</creatorcontrib><creatorcontrib>Miyazaki, Tadaaki</creatorcontrib><title>Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.</description><subject>Acids</subject><subject>Adipose tissue</subject><subject>Aging</subject><subject>Aging (artificial)</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>Antioxidant Response Elements</subject><subject>Antioxidants</subject><subject>Arteriosclerosis</subject><subject>Assaying</subject><subject>Atherosclerosis</subject><subject>Augmentation</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Cadmium</subject><subject>Cancer</subject><subject>Carbon dioxide</subject><subject>Cardiovascular diseases</subject><subject>Catalase</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cellular signal transduction</subject><subject>Centrifugation</subject><subject>Chemokines</subject><subject>Chemotherapy</subject><subject>Computer programs</subject><subject>Cytotoxicity</subject><subject>Diet</subject><subject>Disease</subject><subject>Distilled water</subject><subject>Embryos</subject><subject>Enzymes</subject><subject>Feeding</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - microbiology</subject><subject>Fluorescence</subject><subject>Food</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Immunoglobulin A</subject><subject>Immunology</subject><subject>Ingredients</subject><subject>Inhibition</subject><subject>Intestine</subject><subject>Isoforms</subject><subject>JNK Mitogen-Activated Protein Kinases - metabolism</subject><subject>JNK protein</subject><subject>Kinases</subject><subject>Lactobacillus</subject><subject>Lactobacillus gasseri - metabolism</subject><subject>Life span</subject><subject>Liver diseases</subject><subject>MAP Kinase Signaling System</subject><subject>Medicine</subject><subject>Mice</subject><subject>Microorganisms</subject><subject>Milk</subject><subject>NF-E2-Related Factor 2 - genetics</subject><subject>NF-E2-Related Factor 2 - metabolism</subject><subject>NIH 3T3 Cells</subject><subject>Ostomy</subject><subject>Oxidative Stress</subject><subject>Oxygen</subject><subject>Paraquat</subject><subject>Pharmacology</subject><subject>Phosphates</subject><subject>Physiological aspects</subject><subject>Probiotics</subject><subject>Probiotics - metabolism</subject><subject>Protein kinase</subject><subject>Reactive oxygen species</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Senescence</subject><subject>Signal Transduction</subject><subject>Snow</subject><subject>Toxicity</subject><subject>Transcription factors</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk22L1DAQx4so3nn6DUQLguiLXfPQpukb4Tx8WFg48U7fhmmadLO0zZqkx_ntTXd7x1buhQSSMPnNf5LJTJK8xGiJaYE_bO3gemiXO9urJcJFgRF7lJzikpIFI4g-PtqfJM-83yKUU87Y0-SE8KykJSlOE_3d2aBkMDcqVVrHnU-hr1M99NFoY4C0U3IDvfGdT61O1yCDrUCath182oD3ypn06tM1QXmeQgOm9yG1t6aGvagPTnn_PHmiofXqxbSeJT-_fL6--LZYX35dXZyvF7KgLCx4JssSeIVIrvKM0ZyhDGOWMUKLHNEiq-pCVaXiuK44z2muSVkrTgDXQDVU9Cx5fdDdtdaLKUVeYF5yhBHFJBKrA1Fb2IqdMx24P8KCEXuDdY0AF4xslcCqRrgcY8g601xxYEzyQuMCZ4RQiFofp2hD1alaqj44aGei85PebERjb0SeEcbyIgq8mwSc_T0oH0RnvFRtC72yw_7eJUYRHO_95h_04ddNVAPxAabXNsaVo6g4z0qc0ziN1PIBKo5adUbGetIm2mcO72cOkQnqNjQweC9WVz_-n738NWffHrEbBW3YeNsOY-X5OZgdQOms907p-yRjJMZ2uMuGGNtBTO0Q3V4df9C9013907_T4wQ6</recordid><startdate>20170511</startdate><enddate>20170511</enddate><creator>Kobatake, Eiji</creator><creator>Nakagawa, Hisako</creator><creator>Seki, Takahiro</creator><creator>Miyazaki, Tadaaki</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170511</creationdate><title>Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress</title><author>Kobatake, Eiji ; Nakagawa, Hisako ; Seki, Takahiro ; Miyazaki, Tadaaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c736t-84c99a8b025e546356041164623750374bd7eb9e81db88535f29de82a1da3fab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acids</topic><topic>Adipose tissue</topic><topic>Aging</topic><topic>Aging (artificial)</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>Antioxidant Response Elements</topic><topic>Antioxidants</topic><topic>Arteriosclerosis</topic><topic>Assaying</topic><topic>Atherosclerosis</topic><topic>Augmentation</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Cadmium</topic><topic>Cancer</topic><topic>Carbon dioxide</topic><topic>Cardiovascular diseases</topic><topic>Catalase</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cellular signal transduction</topic><topic>Centrifugation</topic><topic>Chemokines</topic><topic>Chemotherapy</topic><topic>Computer programs</topic><topic>Cytotoxicity</topic><topic>Diet</topic><topic>Disease</topic><topic>Distilled water</topic><topic>Embryos</topic><topic>Enzymes</topic><topic>Feeding</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - microbiology</topic><topic>Fluorescence</topic><topic>Food</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Immunoglobulin A</topic><topic>Immunology</topic><topic>Ingredients</topic><topic>Inhibition</topic><topic>Intestine</topic><topic>Isoforms</topic><topic>JNK Mitogen-Activated Protein Kinases - metabolism</topic><topic>JNK protein</topic><topic>Kinases</topic><topic>Lactobacillus</topic><topic>Lactobacillus gasseri - metabolism</topic><topic>Life span</topic><topic>Liver diseases</topic><topic>MAP Kinase Signaling System</topic><topic>Medicine</topic><topic>Mice</topic><topic>Microorganisms</topic><topic>Milk</topic><topic>NF-E2-Related Factor 2 - genetics</topic><topic>NF-E2-Related Factor 2 - metabolism</topic><topic>NIH 3T3 Cells</topic><topic>Ostomy</topic><topic>Oxidative Stress</topic><topic>Oxygen</topic><topic>Paraquat</topic><topic>Pharmacology</topic><topic>Phosphates</topic><topic>Physiological aspects</topic><topic>Probiotics</topic><topic>Probiotics - metabolism</topic><topic>Protein kinase</topic><topic>Reactive oxygen species</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Senescence</topic><topic>Signal Transduction</topic><topic>Snow</topic><topic>Toxicity</topic><topic>Transcription factors</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobatake, Eiji</creatorcontrib><creatorcontrib>Nakagawa, Hisako</creatorcontrib><creatorcontrib>Seki, Takahiro</creatorcontrib><creatorcontrib>Miyazaki, Tadaaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobatake, Eiji</au><au>Nakagawa, Hisako</au><au>Seki, Takahiro</au><au>Miyazaki, Tadaaki</au><au>Pizzo, Salvatore V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-05-11</date><risdate>2017</risdate><volume>12</volume><issue>5</issue><spage>e0177106</spage><epage>e0177106</epage><pages>e0177106-e0177106</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28493927</pmid><doi>10.1371/journal.pone.0177106</doi><tpages>e0177106</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-05, Vol.12 (5), p.e0177106-e0177106 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1898010312 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Adipose tissue Aging Aging (artificial) Allergic diseases Animals Antioxidant Response Elements Antioxidants Arteriosclerosis Assaying Atherosclerosis Augmentation Bacteria Biochemistry Biology and Life Sciences Biotechnology Cadmium Cancer Carbon dioxide Cardiovascular diseases Catalase Cell growth Cell Proliferation Cells, Cultured Cellular signal transduction Centrifugation Chemokines Chemotherapy Computer programs Cytotoxicity Diet Disease Distilled water Embryos Enzymes Feeding Fibroblasts - cytology Fibroblasts - metabolism Fibroblasts - microbiology Fluorescence Food Gene expression Glucose Heart Heart diseases Immunoglobulin A Immunology Ingredients Inhibition Intestine Isoforms JNK Mitogen-Activated Protein Kinases - metabolism JNK protein Kinases Lactobacillus Lactobacillus gasseri - metabolism Life span Liver diseases MAP Kinase Signaling System Medicine Mice Microorganisms Milk NF-E2-Related Factor 2 - genetics NF-E2-Related Factor 2 - metabolism NIH 3T3 Cells Ostomy Oxidative Stress Oxygen Paraquat Pharmacology Phosphates Physiological aspects Probiotics Probiotics - metabolism Protein kinase Reactive oxygen species Research and Analysis Methods Rodents Senescence Signal Transduction Snow Toxicity Transcription factors Viruses |
| title | Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T09%3A40%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20effects%20and%20functional%20mechanisms%20of%20Lactobacillus%20gasseri%20SBT2055%20against%20oxidative%20stress&rft.jtitle=PloS%20one&rft.au=Kobatake,%20Eiji&rft.date=2017-05-11&rft.volume=12&rft.issue=5&rft.spage=e0177106&rft.epage=e0177106&rft.pages=e0177106-e0177106&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0177106&rft_dat=%3Cgale_plos_%3EA491534912%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1898010312&rft_id=info:pmid/28493927&rft_galeid=A491534912&rft_doaj_id=oai_doaj_org_article_1ed0195f29cd4f8e8a66c87f1714223a&rfr_iscdi=true |