Narrow microtunnel technology for the isolation and precise identification of axonal communication among distinct hippocampal subregion networks
Communication between different sub regions of the hippocampus is fundamental to learning and memory. However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have...
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| creator | Narula, Udit Ruiz, Andres McQuaide, McKinley DeMarse, Thomas B Wheeler, Bruce C Brewer, Gregory J |
| description | Communication between different sub regions of the hippocampus is fundamental to learning and memory. However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have begun to approach this problem but the commonly used 10 μm wide tunnels frequently measure signals from multiple axons. To reduce this complexity, we compared polydimethylsiloxane (PDMS) microtunnel devices each with a separate tunnel width of 2.5, 5 or 10 μm bridging two wells aligned over a multi electrode array (MEA). Primary rat neurons were grown in the chambers with neurons from the dentate gyrus on one side and hippocampal CA3 on the other. After 2-3 weeks of culture, spontaneous activity in the axons inside the tunnels was recorded. We report electrophysiological, exploratory data analysis for feature clustering and visual evidence to support the expectation that 2.5 μm wide tunnels have fewer axons per tunnel and therefore more clearly delineated signals than 10 or 5 μm wide tunnels. Several measures indicated that fewer axons per electrode enabled more accurate detection of spikes. A clustering analysis comparing the variations of spike height and width for different tunnel widths revealed tighter clusters representing unique spikes with less height and width variation when measured in narrow tunnels. Wider tunnels tended toward more diffuse clusters from a continuum of spike heights and widths. Standard deviations for multiple cluster measures, such as Average Dissimilarity, Silhouette Value (S) and Separation Factor (average dissimilarity/S value), support a conclusion that 2.5 μm wide tunnels containing fewer axons enable more precise determination of individual action potential peaks, their propagation direction, timing, and information transfer between sub networks. |
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However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have begun to approach this problem but the commonly used 10 μm wide tunnels frequently measure signals from multiple axons. To reduce this complexity, we compared polydimethylsiloxane (PDMS) microtunnel devices each with a separate tunnel width of 2.5, 5 or 10 μm bridging two wells aligned over a multi electrode array (MEA). Primary rat neurons were grown in the chambers with neurons from the dentate gyrus on one side and hippocampal CA3 on the other. After 2-3 weeks of culture, spontaneous activity in the axons inside the tunnels was recorded. We report electrophysiological, exploratory data analysis for feature clustering and visual evidence to support the expectation that 2.5 μm wide tunnels have fewer axons per tunnel and therefore more clearly delineated signals than 10 or 5 μm wide tunnels. Several measures indicated that fewer axons per electrode enabled more accurate detection of spikes. A clustering analysis comparing the variations of spike height and width for different tunnel widths revealed tighter clusters representing unique spikes with less height and width variation when measured in narrow tunnels. Wider tunnels tended toward more diffuse clusters from a continuum of spike heights and widths. Standard deviations for multiple cluster measures, such as Average Dissimilarity, Silhouette Value (S) and Separation Factor (average dissimilarity/S value), support a conclusion that 2.5 μm wide tunnels containing fewer axons enable more precise determination of individual action potential peaks, their propagation direction, timing, and information transfer between sub networks.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0176868</identifier><identifier>PMID: 28493886</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Action potential ; Action Potentials - physiology ; Actuators ; Alternating current ; Animals ; Axon guidance ; Axonogenesis ; Axons ; Axons - physiology ; Bioengineering ; Biology and Life Sciences ; Biomedical engineering ; Brain ; Brain damage ; Cell culture ; Central nervous system ; Chambers ; Circuits ; Clustering ; Clusters ; Collagen ; Communication ; Compartments ; Computer networks ; Cortex ; Coupling ; Data acquisition ; Data analysis ; Data collection ; Data processing ; Dendrites ; Dentate gyrus ; Disease transmission ; Elastomers ; Electrodes ; Engineering ; Firing pattern ; Hippocampus ; Hippocampus (Brain) ; Hippocampus - physiology ; Identification ; Interfaces ; Interference ; Learning ; Lithography ; Medical examination ; Medicine and Health Sciences ; Memory ; Memory consolidation ; Methods ; Micro-Electrical-Mechanical Systems - methods ; Microelectrodes ; Microscopy, Confocal ; Movement disorders ; Multiple sclerosis ; Nerve Net - physiology ; Nervous system ; Neural circuitry ; Neural Conduction - physiology ; Neural networks ; Neurodegenerative diseases ; Neurology ; Neurons ; Neurosciences ; Parkinson's disease ; Pharmacology ; Physical Sciences ; Populations ; Propagation ; Psychological aspects ; Rats, Sprague-Dawley ; Recording ; Research and Analysis Methods ; Sensors ; Separation ; Technology ; Tunnels ; Visual perception</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0176868-e0176868</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Narula et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Narula et al 2017 Narula et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-123421d4d969499cd40904829c0cc5f05be982d65bcd6dba892c36af134a94543</citedby><cites>FETCH-LOGICAL-c692t-123421d4d969499cd40904829c0cc5f05be982d65bcd6dba892c36af134a94543</cites><orcidid>0000-0002-8535-1832</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426613/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426613/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28493886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narula, Udit</creatorcontrib><creatorcontrib>Ruiz, Andres</creatorcontrib><creatorcontrib>McQuaide, McKinley</creatorcontrib><creatorcontrib>DeMarse, Thomas B</creatorcontrib><creatorcontrib>Wheeler, Bruce C</creatorcontrib><creatorcontrib>Brewer, Gregory J</creatorcontrib><title>Narrow microtunnel technology for the isolation and precise identification of axonal communication among distinct hippocampal subregion networks</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Communication between different sub regions of the hippocampus is fundamental to learning and memory. However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have begun to approach this problem but the commonly used 10 μm wide tunnels frequently measure signals from multiple axons. To reduce this complexity, we compared polydimethylsiloxane (PDMS) microtunnel devices each with a separate tunnel width of 2.5, 5 or 10 μm bridging two wells aligned over a multi electrode array (MEA). Primary rat neurons were grown in the chambers with neurons from the dentate gyrus on one side and hippocampal CA3 on the other. After 2-3 weeks of culture, spontaneous activity in the axons inside the tunnels was recorded. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narula, Udit</au><au>Ruiz, Andres</au><au>McQuaide, McKinley</au><au>DeMarse, Thomas B</au><au>Wheeler, Bruce C</au><au>Brewer, Gregory J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Narrow microtunnel technology for the isolation and precise identification of axonal communication among distinct hippocampal subregion networks</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-05-11</date><risdate>2017</risdate><volume>12</volume><issue>5</issue><spage>e0176868</spage><epage>e0176868</epage><pages>e0176868-e0176868</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Communication between different sub regions of the hippocampus is fundamental to learning and memory. However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have begun to approach this problem but the commonly used 10 μm wide tunnels frequently measure signals from multiple axons. To reduce this complexity, we compared polydimethylsiloxane (PDMS) microtunnel devices each with a separate tunnel width of 2.5, 5 or 10 μm bridging two wells aligned over a multi electrode array (MEA). Primary rat neurons were grown in the chambers with neurons from the dentate gyrus on one side and hippocampal CA3 on the other. After 2-3 weeks of culture, spontaneous activity in the axons inside the tunnels was recorded. We report electrophysiological, exploratory data analysis for feature clustering and visual evidence to support the expectation that 2.5 μm wide tunnels have fewer axons per tunnel and therefore more clearly delineated signals than 10 or 5 μm wide tunnels. Several measures indicated that fewer axons per electrode enabled more accurate detection of spikes. A clustering analysis comparing the variations of spike height and width for different tunnel widths revealed tighter clusters representing unique spikes with less height and width variation when measured in narrow tunnels. Wider tunnels tended toward more diffuse clusters from a continuum of spike heights and widths. Standard deviations for multiple cluster measures, such as Average Dissimilarity, Silhouette Value (S) and Separation Factor (average dissimilarity/S value), support a conclusion that 2.5 μm wide tunnels containing fewer axons enable more precise determination of individual action potential peaks, their propagation direction, timing, and information transfer between sub networks.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28493886</pmid><doi>10.1371/journal.pone.0176868</doi><tpages>e0176868</tpages><orcidid>https://orcid.org/0000-0002-8535-1832</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1897800109 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Action potential Action Potentials - physiology Actuators Alternating current Animals Axon guidance Axonogenesis Axons Axons - physiology Bioengineering Biology and Life Sciences Biomedical engineering Brain Brain damage Cell culture Central nervous system Chambers Circuits Clustering Clusters Collagen Communication Compartments Computer networks Cortex Coupling Data acquisition Data analysis Data collection Data processing Dendrites Dentate gyrus Disease transmission Elastomers Electrodes Engineering Firing pattern Hippocampus Hippocampus (Brain) Hippocampus - physiology Identification Interfaces Interference Learning Lithography Medical examination Medicine and Health Sciences Memory Memory consolidation Methods Micro-Electrical-Mechanical Systems - methods Microelectrodes Microscopy, Confocal Movement disorders Multiple sclerosis Nerve Net - physiology Nervous system Neural circuitry Neural Conduction - physiology Neural networks Neurodegenerative diseases Neurology Neurons Neurosciences Parkinson's disease Pharmacology Physical Sciences Populations Propagation Psychological aspects Rats, Sprague-Dawley Recording Research and Analysis Methods Sensors Separation Technology Tunnels Visual perception |
| title | Narrow microtunnel technology for the isolation and precise identification of axonal communication among distinct hippocampal subregion networks |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T12%3A22%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Narrow%20microtunnel%20technology%20for%20the%20isolation%20and%20precise%20identification%20of%20axonal%20communication%20among%20distinct%20hippocampal%20subregion%20networks&rft.jtitle=PloS%20one&rft.au=Narula,%20Udit&rft.date=2017-05-11&rft.volume=12&rft.issue=5&rft.spage=e0176868&rft.epage=e0176868&rft.pages=e0176868-e0176868&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0176868&rft_dat=%3Cgale_plos_%3EA491534913%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1897800109&rft_id=info:pmid/28493886&rft_galeid=A491534913&rft_doaj_id=oai_doaj_org_article_0299f10e90304692b6e2dd038362f375&rfr_iscdi=true |