The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)
The necrotrophic pathogen Rhizoctonia solani is one of the most economically important soil-borne pathogens of crop plants. Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Cur...
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| description | The necrotrophic pathogen Rhizoctonia solani is one of the most economically important soil-borne pathogens of crop plants. Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Currently, there is lack of information regarding the molecular responses in R. solani during the pathogenic interaction between the necrotrophic soil-borne pathogen and its host plant. The genome of R. solani AG1-IB (isolate 7/3/14) was recently established to obtain insights into its putative pathogenicity determinants. In this study, the transcriptional activity of R. solani AG1-IB was followed during the course of its pathogenic interaction with the host plant lettuce under controlled conditions. Based on visual observations, three distinct pathogen-host interaction zones on lettuce leaves were defined which covered different phases of disease progression on tissue inoculated with the AG1-IB (isolate 7/3/14). The zones were defined as: Zone 1-symptomless, Zone 2-light brown discoloration, and Zone 3-dark brown, necrotic lesions. Differences in R. solani hyphae structure in these three zones were investigated by microscopic observation. Transcriptional activity within these three interaction zones was used to represent the course of R. solani disease progression applying high-throughput RNA sequencing (RNA-Seq) analysis of samples collected from each Zone. The resulting three transcriptome data sets were analyzed for their highest expressed genes and for differentially transcribed genes between the respective interaction zones. Among the highest expressed genes was a group of not previously described genes which were transcribed exclusively during early stages of interaction, in Zones 1 and 2. Previously described importance of up-regulation in R. solani agglutinin genes during disease progression could be further confirmed; here, the corresponding genes exhibited extremely high transcription levels. Most differentially higher expressed transcripts were found within Zone 2. In Zone 3, the zone with the strongest degree of interaction, gene transcripts indicative of apoptotic activity were highly abundant. The transcriptome data presented in this work support previous models of the disease and interaction cycle of R. solani and lettuce and may influence effective techniques for control of this pathogen. |
| doi_str_mv | 10.1371/journal.pone.0177278 |
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Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Currently, there is lack of information regarding the molecular responses in R. solani during the pathogenic interaction between the necrotrophic soil-borne pathogen and its host plant. The genome of R. solani AG1-IB (isolate 7/3/14) was recently established to obtain insights into its putative pathogenicity determinants. In this study, the transcriptional activity of R. solani AG1-IB was followed during the course of its pathogenic interaction with the host plant lettuce under controlled conditions. Based on visual observations, three distinct pathogen-host interaction zones on lettuce leaves were defined which covered different phases of disease progression on tissue inoculated with the AG1-IB (isolate 7/3/14). The zones were defined as: Zone 1-symptomless, Zone 2-light brown discoloration, and Zone 3-dark brown, necrotic lesions. Differences in R. solani hyphae structure in these three zones were investigated by microscopic observation. Transcriptional activity within these three interaction zones was used to represent the course of R. solani disease progression applying high-throughput RNA sequencing (RNA-Seq) analysis of samples collected from each Zone. The resulting three transcriptome data sets were analyzed for their highest expressed genes and for differentially transcribed genes between the respective interaction zones. Among the highest expressed genes was a group of not previously described genes which were transcribed exclusively during early stages of interaction, in Zones 1 and 2. Previously described importance of up-regulation in R. solani agglutinin genes during disease progression could be further confirmed; here, the corresponding genes exhibited extremely high transcription levels. Most differentially higher expressed transcripts were found within Zone 2. In Zone 3, the zone with the strongest degree of interaction, gene transcripts indicative of apoptotic activity were highly abundant. The transcriptome data presented in this work support previous models of the disease and interaction cycle of R. solani and lettuce and may influence effective techniques for control of this pathogen.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0177278</identifier><identifier>PMID: 28486484</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Apoptosis ; Biology and Life Sciences ; Biotechnology ; Controlled conditions ; Crop diseases ; Data processing ; Discoloration ; Economic importance ; Enzymes ; Fungi ; Gangrene ; Gene expression ; Gene Expression Profiling ; Gene sequencing ; Genes ; Genes, Bacterial ; Genomes ; High-Throughput Nucleotide Sequencing ; Host plants ; Host-Pathogen Interactions ; Hyphae ; Infections ; Lactuca - microbiology ; Lactuca sativa ; Leaves ; Lesions ; Lettuce ; Medicine and Health Sciences ; Pathogenesis ; Pathogenicity ; Pathogens ; Plant diseases ; Plant species ; Proteins ; Rhizoctonia - genetics ; Rhizoctonia solani ; Ribonucleic acid ; Rice ; RNA ; RNA, Plant - genetics ; Rot ; Transcription ; Transcriptome ; Vegetables ; Visual observation</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0177278</ispartof><rights>2017 Verwaaijen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Verwaaijen et al 2017 Verwaaijen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-9a0692738e56415bfcce97c5c6177f4a0cf50de7febf08305c65e079e0829bd93</citedby><cites>FETCH-LOGICAL-c526t-9a0692738e56415bfcce97c5c6177f4a0cf50de7febf08305c65e079e0829bd93</cites><orcidid>0000-0003-4830-310X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423683/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423683/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28486484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>van Overbeek, Leonard Simon</contributor><creatorcontrib>Verwaaijen, Bart</creatorcontrib><creatorcontrib>Wibberg, Daniel</creatorcontrib><creatorcontrib>Kröber, Magdalena</creatorcontrib><creatorcontrib>Winkler, Anika</creatorcontrib><creatorcontrib>Zrenner, Rita</creatorcontrib><creatorcontrib>Bednarz, Hanna</creatorcontrib><creatorcontrib>Niehaus, Karsten</creatorcontrib><creatorcontrib>Grosch, Rita</creatorcontrib><creatorcontrib>Pühler, Alfred</creatorcontrib><creatorcontrib>Schlüter, Andreas</creatorcontrib><title>The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The necrotrophic pathogen Rhizoctonia solani is one of the most economically important soil-borne pathogens of crop plants. Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Currently, there is lack of information regarding the molecular responses in R. solani during the pathogenic interaction between the necrotrophic soil-borne pathogen and its host plant. The genome of R. solani AG1-IB (isolate 7/3/14) was recently established to obtain insights into its putative pathogenicity determinants. In this study, the transcriptional activity of R. solani AG1-IB was followed during the course of its pathogenic interaction with the host plant lettuce under controlled conditions. Based on visual observations, three distinct pathogen-host interaction zones on lettuce leaves were defined which covered different phases of disease progression on tissue inoculated with the AG1-IB (isolate 7/3/14). The zones were defined as: Zone 1-symptomless, Zone 2-light brown discoloration, and Zone 3-dark brown, necrotic lesions. Differences in R. solani hyphae structure in these three zones were investigated by microscopic observation. Transcriptional activity within these three interaction zones was used to represent the course of R. solani disease progression applying high-throughput RNA sequencing (RNA-Seq) analysis of samples collected from each Zone. The resulting three transcriptome data sets were analyzed for their highest expressed genes and for differentially transcribed genes between the respective interaction zones. Among the highest expressed genes was a group of not previously described genes which were transcribed exclusively during early stages of interaction, in Zones 1 and 2. Previously described importance of up-regulation in R. solani agglutinin genes during disease progression could be further confirmed; here, the corresponding genes exhibited extremely high transcription levels. Most differentially higher expressed transcripts were found within Zone 2. In Zone 3, the zone with the strongest degree of interaction, gene transcripts indicative of apoptotic activity were highly abundant. The transcriptome data presented in this work support previous models of the disease and interaction cycle of R. solani and lettuce and may influence effective techniques for control of this pathogen.</description><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Controlled conditions</subject><subject>Crop diseases</subject><subject>Data processing</subject><subject>Discoloration</subject><subject>Economic importance</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Gangrene</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genomes</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Host plants</subject><subject>Host-Pathogen Interactions</subject><subject>Hyphae</subject><subject>Infections</subject><subject>Lactuca - microbiology</subject><subject>Lactuca sativa</subject><subject>Leaves</subject><subject>Lesions</subject><subject>Lettuce</subject><subject>Medicine and Health Sciences</subject><subject>Pathogenesis</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant species</subject><subject>Proteins</subject><subject>Rhizoctonia - genetics</subject><subject>Rhizoctonia solani</subject><subject>Ribonucleic acid</subject><subject>Rice</subject><subject>RNA</subject><subject>RNA, Plant - genetics</subject><subject>Rot</subject><subject>Transcription</subject><subject>Transcriptome</subject><subject>Vegetables</subject><subject>Visual observation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkstuEzEUhkcIREvhDRBYYpMukvg2vmwqlQpKpEhIqKwtx3MmcTQZB9tTBBteHaeZVi1i5cv5z3cu-qvqLcEzwiSZb8MQe9vN9qGHGSZSUqmeVadEMzoVFLPnj-4n1auUthjXTAnxsjqhiivBFT-t_txsAH3b-N_B5dB7i1LobO_R5TWZLj6iiT-8MyA5Z3PCz1GOtk8u-n0OO0DNEH2_Rr7PEK3LPvTop88blAt0E1JG-wLLqIOcBwdosiyiwZUiNvtbi5az89fVi9Z2Cd6M51n1_fOnm6sv0-XX68XV5XLqairyVFssNJVMQS04qVetc6Clq50og7fcYtfWuAHZwqrFiuESqAFLDVhRvWo0O6veH7n7LiQz7i4ZorRQnJFaFcXiqGiC3Zp99Dsbf5lgvbn7CHFtbMzedWCEEtgJBaW-5ivaat1QwK7GVDZCC1dYF2O1YbWDxkFf9tY9gT6N9H5j1uHW1JwyoVgBTEZADD8GSNnsfHLQlXVCGO761lIzhnmRfvhH-v_p-FHlYkgpQvvQDMHm4Kf7LHPwkxn9VNLePR7kIeneQOwvPUjIhw</recordid><startdate>20170509</startdate><enddate>20170509</enddate><creator>Verwaaijen, Bart</creator><creator>Wibberg, Daniel</creator><creator>Kröber, Magdalena</creator><creator>Winkler, Anika</creator><creator>Zrenner, Rita</creator><creator>Bednarz, Hanna</creator><creator>Niehaus, Karsten</creator><creator>Grosch, Rita</creator><creator>Pühler, Alfred</creator><creator>Schlüter, Andreas</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-4830-310X</orcidid></search><sort><creationdate>20170509</creationdate><title>The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)</title><author>Verwaaijen, Bart ; Wibberg, Daniel ; Kröber, Magdalena ; Winkler, Anika ; Zrenner, Rita ; Bednarz, Hanna ; Niehaus, Karsten ; Grosch, Rita ; Pühler, Alfred ; Schlüter, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-9a0692738e56415bfcce97c5c6177f4a0cf50de7febf08305c65e079e0829bd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Apoptosis</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Controlled conditions</topic><topic>Crop diseases</topic><topic>Data processing</topic><topic>Discoloration</topic><topic>Economic importance</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Gangrene</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genomes</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Host plants</topic><topic>Host-Pathogen Interactions</topic><topic>Hyphae</topic><topic>Infections</topic><topic>Lactuca - 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Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Currently, there is lack of information regarding the molecular responses in R. solani during the pathogenic interaction between the necrotrophic soil-borne pathogen and its host plant. The genome of R. solani AG1-IB (isolate 7/3/14) was recently established to obtain insights into its putative pathogenicity determinants. In this study, the transcriptional activity of R. solani AG1-IB was followed during the course of its pathogenic interaction with the host plant lettuce under controlled conditions. Based on visual observations, three distinct pathogen-host interaction zones on lettuce leaves were defined which covered different phases of disease progression on tissue inoculated with the AG1-IB (isolate 7/3/14). The zones were defined as: Zone 1-symptomless, Zone 2-light brown discoloration, and Zone 3-dark brown, necrotic lesions. Differences in R. solani hyphae structure in these three zones were investigated by microscopic observation. Transcriptional activity within these three interaction zones was used to represent the course of R. solani disease progression applying high-throughput RNA sequencing (RNA-Seq) analysis of samples collected from each Zone. The resulting three transcriptome data sets were analyzed for their highest expressed genes and for differentially transcribed genes between the respective interaction zones. Among the highest expressed genes was a group of not previously described genes which were transcribed exclusively during early stages of interaction, in Zones 1 and 2. Previously described importance of up-regulation in R. solani agglutinin genes during disease progression could be further confirmed; here, the corresponding genes exhibited extremely high transcription levels. Most differentially higher expressed transcripts were found within Zone 2. In Zone 3, the zone with the strongest degree of interaction, gene transcripts indicative of apoptotic activity were highly abundant. The transcriptome data presented in this work support previous models of the disease and interaction cycle of R. solani and lettuce and may influence effective techniques for control of this pathogen.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28486484</pmid><doi>10.1371/journal.pone.0177278</doi><orcidid>https://orcid.org/0000-0003-4830-310X</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1896843158 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Apoptosis Biology and Life Sciences Biotechnology Controlled conditions Crop diseases Data processing Discoloration Economic importance Enzymes Fungi Gangrene Gene expression Gene Expression Profiling Gene sequencing Genes Genes, Bacterial Genomes High-Throughput Nucleotide Sequencing Host plants Host-Pathogen Interactions Hyphae Infections Lactuca - microbiology Lactuca sativa Leaves Lesions Lettuce Medicine and Health Sciences Pathogenesis Pathogenicity Pathogens Plant diseases Plant species Proteins Rhizoctonia - genetics Rhizoctonia solani Ribonucleic acid Rice RNA RNA, Plant - genetics Rot Transcription Transcriptome Vegetables Visual observation |
| title | The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T16%3A13%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Rhizoctonia%20solani%20AG1-IB%20(isolate%207/3/14)%20transcriptome%20during%20interaction%20with%20the%20host%20plant%20lettuce%20(Lactuca%20sativa%20L.)&rft.jtitle=PloS%20one&rft.au=Verwaaijen,%20Bart&rft.date=2017-05-09&rft.volume=12&rft.issue=5&rft.spage=e0177278&rft.pages=e0177278-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0177278&rft_dat=%3Cproquest_plos_%3E1899793304%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1896843158&rft_id=info:pmid/28486484&rft_doaj_id=oai_doaj_org_article_6860c68ec5c94b2f99d2e0c5027d696c&rfr_iscdi=true |