Poa secunda local collections and commercial releases: A genotypic evaluation

The genetics of native plants influence the success of ecological restoration, yet genetic variability of local seed collections and commercial seed releases remains unclear for most taxa. Poa secunda, a common native grass species in Intermountain West grasslands and a frequent component of restora...

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Veröffentlicht in:PloS one 2017-04, Vol.12 (4), p.e0173221-e0173221
Hauptverfasser: Shaw, Alanna N, Mummey, Daniel L
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description The genetics of native plants influence the success of ecological restoration, yet genetic variability of local seed collections and commercial seed releases remains unclear for most taxa. Poa secunda, a common native grass species in Intermountain West grasslands and a frequent component of restoration seed mixes, is one such species. Here, we evaluate the genetic variation of local Poa secunda collections in the context of wild populations and commercial seed releases. We evaluated AFLP markers for seven Poa secunda collections made over a 4000-hectare area and four commercial releases (High Plains, MT-1, Opportunity, and Sherman). We compare the genetic distance and distribution of genetic variation within and between local collections and commercial releases. The extent and patterns of genetic variation in our local collections indicate subtle site differences with most variation occurring within rather than between collections. Identical genetic matches were usually, but not always, found within 5 m2 collection sites. Our results suggest that the genetic variation in two Poa secunda releases (High Plains and MT-1) is similar to our local collections. Our results affirm that guidelines for Poa secunda seed collection should follow recommendations for selfing species, by collecting from many sites over large individual sites.
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Poa secunda, a common native grass species in Intermountain West grasslands and a frequent component of restoration seed mixes, is one such species. Here, we evaluate the genetic variation of local Poa secunda collections in the context of wild populations and commercial seed releases. We evaluated AFLP markers for seven Poa secunda collections made over a 4000-hectare area and four commercial releases (High Plains, MT-1, Opportunity, and Sherman). We compare the genetic distance and distribution of genetic variation within and between local collections and commercial releases. The extent and patterns of genetic variation in our local collections indicate subtle site differences with most variation occurring within rather than between collections. Identical genetic matches were usually, but not always, found within 5 m2 collection sites. Our results suggest that the genetic variation in two Poa secunda releases (High Plains and MT-1) is similar to our local collections. Our results affirm that guidelines for Poa secunda seed collection should follow recommendations for selfing species, by collecting from many sites over large individual sites.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28369130</pmid><doi>10.1371/journal.pone.0173221</doi><tpages>e0173221</tpages><oa>free_for_read</oa></addata></record>
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subjects Adaptation, Physiological - genetics
Agriculture
Amplified fragment length polymorphism
Amplified Fragment Length Polymorphism Analysis
Apomixis
Bioinformatics
Biology and Life Sciences
Collection
Collections
Conservation of Natural Resources
Crop science
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
DNA, Plant - isolation & purification
Earth Sciences
Ecological effects
Ecological restoration
Ecology
Ecology and Environmental Sciences
Ecosystem
Genetic distance
Genetic diversity
Genetic variability
Genetic Variation
Genetics
Genotype
Germplasm
Grasslands
High plains
Indigenous plants
Mitochondrial DNA
Montana
Physical Sciences
Poa - genetics
Poa - physiology
Population
Research and Analysis Methods
Restoration
Seed collection
Seeds
Seeds - genetics
Species
Taxa
title Poa secunda local collections and commercial releases: A genotypic evaluation
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