Sampling and detection of airborne influenza virus towards point-of-care applications
Airborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of th...
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| Veröffentlicht in: | PloS one 2017-03, Vol.12 (3), p.e0174314 |
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| creator | Ladhani, Laila Pardon, Gaspard Meeuws, Hanne van Wesenbeeck, Liesbeth Schmidt, Kristiane Stuyver, Lieven van der Wijngaart, Wouter |
| description | Airborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler. |
| doi_str_mv | 10.1371/journal.pone.0174314 |
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Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0174314</identifier><identifier>PMID: 28350811</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aerosols ; Aerosols - analysis ; Air Microbiology ; Analysis ; Bioaerosols ; Bioassays ; Biological assays ; Biology and life sciences ; Biosensors ; Care and treatment ; Collection ; Design ; Dilution ; Disease transmission ; Efficiency ; Electrostatic precipitation ; Environmental Monitoring - instrumentation ; Epidemics ; Equipment Design ; Humans ; Infections ; Influenza ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H1N1 Subtype - isolation & purification ; Influenza A Virus, H3N2 Subtype - genetics ; Influenza A Virus, H3N2 Subtype - isolation & purification ; Influenza viruses ; Influenza, Human - virology ; Medicine and Health Sciences ; Microorganisms ; Pandemics ; Physical Sciences ; Point-of-Care Systems ; Polymerase chain reaction ; Precipitation (Meteorology) ; Research and analysis methods ; Ribonucleic acid ; RNA ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Samplers ; Sampling ; Sampling methods ; Technology application ; Virology ; Viruses</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0174314</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Ladhani et al. 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Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28350811</pmid><doi>10.1371/journal.pone.0174314</doi><tpages>e0174314</tpages><orcidid>https://orcid.org/0000-0001-9177-1174</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-03, Vol.12 (3), p.e0174314 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SWEPUB Freely available online; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aerosols Aerosols - analysis Air Microbiology Analysis Bioaerosols Bioassays Biological assays Biology and life sciences Biosensors Care and treatment Collection Design Dilution Disease transmission Efficiency Electrostatic precipitation Environmental Monitoring - instrumentation Epidemics Equipment Design Humans Infections Influenza Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - isolation & purification Influenza viruses Influenza, Human - virology Medicine and Health Sciences Microorganisms Pandemics Physical Sciences Point-of-Care Systems Polymerase chain reaction Precipitation (Meteorology) Research and analysis methods Ribonucleic acid RNA RNA, Viral - genetics RNA, Viral - isolation & purification Samplers Sampling Sampling methods Technology application Virology Viruses |
| title | Sampling and detection of airborne influenza virus towards point-of-care applications |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T11%3A18%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sampling%20and%20detection%20of%20airborne%20influenza%20virus%20towards%20point-of-care%20applications&rft.jtitle=PloS%20one&rft.au=Ladhani,%20Laila&rft.date=2017-03-28&rft.volume=12&rft.issue=3&rft.spage=e0174314&rft.pages=e0174314-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0174314&rft_dat=%3Cgale_plos_%3EA487530190%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1882248096&rft_id=info:pmid/28350811&rft_galeid=A487530190&rft_doaj_id=oai_doaj_org_article_316cc61f2f224a51b7d0b9dc41e6b407&rfr_iscdi=true |