Identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by righ-resolution melting analysis

The objective of this study was to standardize the high-resolution melting method for identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by amplification of 18S ribosomal DNA (rDNA) using a single primer pair. The analyses were performed...

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Veröffentlicht in:	PloS one 2017-03, Vol.12 (3), p.e0174168-e0174168
Hauptverfasser:	Fehlberg, Hllytchaikra Ferraz, Maciel, Bianca Mendes, Albuquerque, George Rêgo
Format:	Artikel
Sprache:	eng
Schlagworte:	Abortion Acetal resins Agronomy Amine oxidase (flavin-containing) Amplification Animal health Animals Antibiotic resistance Antibiotics Antigens Apical membrane antigen 1 Assaying B1 gene Biochemistry Biology and Life Sciences Bradyzoites Brain Cadmium Coccidiosis - diagnosis Coccidiosis - parasitology Complementarity Computer programs Concentration (composition) Cryptosporidiosis Cryptosporidiosis - diagnosis Cryptosporidiosis - parasitology Cryptosporidium Cryptosporidium - genetics Cryptosporidium - isolation & purification Denaturation Deoxyribonucleic acid Diagnosis Dilution Disease Diseases DNA DNA methylation DNA, Protozoan - genetics DNA, Protozoan - isolation & purification DNA, Ribosomal - genetics DNA, Ribosomal - isolation & purification Dyes Epidemiology Feces Fluorescence Free water Genetic analysis Genetic testing Genomes Genotypes High resolution Humans Identification Identification and classification Inhibition Intervention Intestine Life cycles Meadows Melting Mice Multiplexing Neospora - genetics Neospora - isolation & purification Nucleic Acid Denaturation Nucleotide sequence Oocysts Parasites Peritoneum pH effects Physical Sciences Polymerase Chain Reaction Protozoa Protozoan Infections - diagnosis Protozoan Infections - parasitology Research and Analysis Methods Ribosomal DNA Sarcocystis Sarcocystis - genetics Sarcocystis - isolation & purification Sarcocystosis - diagnosis Sarcocystosis - parasitology Saturation Sexually transmitted diseases STD Systems analysis Temperature effects Toxoplasma Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasmosis Toxoplasmosis - diagnosis Toxoplasmosis - parasitology Ultraviolet radiation
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