Acute exhaustive aerobic exercise training impair cardiomyocyte function and calcium handling in Sprague-Dawley rats
Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and...
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| description | Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and therefore the aim of this study was to examine cardiomyocyte and molecular responses of the right and left ventricle to an acute bout of exhaustive endurance exercise.
Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run. The contractile function and Ca2+ handling properties in isolated cardiomyocytes, protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban including two of its phosphorylated states (serine 16 and threonine 17), and the mitochondrial respiration in permeabilized cardiac muscle fibers were analyzed.
The exercise group showed a significant reduction in cardiomyocyte fractional shortening (right ventricle 1 Hz and 3 Hz p |
| doi_str_mv | 10.1371/journal.pone.0173449 |
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Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run. The contractile function and Ca2+ handling properties in isolated cardiomyocytes, protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban including two of its phosphorylated states (serine 16 and threonine 17), and the mitochondrial respiration in permeabilized cardiac muscle fibers were analyzed.
The exercise group showed a significant reduction in cardiomyocyte fractional shortening (right ventricle 1 Hz and 3 Hz p<0.001; left ventricle 1 Hz p<0.05), intracellular Ca2+ amplitude (right ventricle 1 and 3 Hz p<0.001; left ventricle 1 Hz p<0.01 and 3 Hz p<0.05) and rate of diastolic Ca2+ decay (right ventricle 1 Hz p<0.001 and 3 Hz p<0.01; left ventricle 1 and 3 Hz p<0.01). Cardiomyocyte relaxation during diastole was only significantly prolonged at 3 Hz in the right ventricle (p<0.05) compared to sedentary controls. We found an increase in phosphorylation of phospholamban at serine 16 and threonine 17 in the left (p<0.05), but not the right, ventricle from exhaustively exercised animals. The protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban was not changed. Furthermore, we found a reduction in maximal oxidative phosphorylation and electron transport system capacities of mitochondrial respiration in the right (p<0.01 and p<0.05, respectively), but not the left ventricle from rats subjected to acute exhaustive treadmill exercise.
Acute exhaustive treadmill exercise is associated with impairment of cardiomyocyte Ca2+ handling and mitochondrial respiration that causes depression in both contraction and diastolic relaxation of cardiomyocytes.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0173449</identifier><identifier>PMID: 28273177</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine triphosphatase ; Aerobic exercises ; Aerobics ; Animals ; Bioenergetics ; Biology and Life Sciences ; Biomarkers ; Ca2+-transporting ATPase ; Calcium ; Calcium (intracellular) ; Calcium (mitochondrial) ; Calcium (reticular) ; Calcium - metabolism ; Calcium ions ; Cardiac muscle ; Cardiology ; Cardiomyocytes ; Cell Membrane Permeability ; Cell Respiration ; Cells, Cultured ; Contraction ; Diastole ; Durability ; Electron transport ; Endurance ; Exercise ; Fitness equipment ; Handling ; Heart ; Heart - physiopathology ; Heart cells ; Heart diseases ; Ion transport ; Male ; Medical imaging ; Medicine ; Medicine and Health Sciences ; Mental depression ; Mitochondria ; Mitochondria, Heart - metabolism ; Muscle contraction ; Muscles ; Myocardial Contraction ; Myocardium - metabolism ; Myocytes, Cardiac - metabolism ; Observations ; Oxidative phosphorylation ; Phospholamban ; Phosphorylation ; Physical Conditioning, Animal ; Physical fitness ; Physical Sciences ; Physiological aspects ; Physiology ; Properties ; Pulmonary arteries ; Rats ; Rats, Sprague-Dawley ; Reduction ; Rehabilitation ; Research and Analysis Methods ; Respiration ; Rodents ; Running ; Sarcoplasmic reticulum ; Science ; Serine ; Studies ; Threonine ; Ventricle ; Working groups ; Workloads</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0173449-e0173449</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Ljones et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Ljones et al 2017 Ljones et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-645d26eed563aabe9add24421f3f35b2d965ce5cfc217e4f48f31fe957d3e74d3</citedby><cites>FETCH-LOGICAL-c791t-645d26eed563aabe9add24421f3f35b2d965ce5cfc217e4f48f31fe957d3e74d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342256/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342256/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28273177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>González, Germán E.</contributor><creatorcontrib>Ljones, Kristine</creatorcontrib><creatorcontrib>Ness, Henning Ofstad</creatorcontrib><creatorcontrib>Solvang-Garten, Karin</creatorcontrib><creatorcontrib>Gaustad, Svein Erik</creatorcontrib><creatorcontrib>Høydal, Morten Andre</creatorcontrib><title>Acute exhaustive aerobic exercise training impair cardiomyocyte function and calcium handling in Sprague-Dawley rats</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and therefore the aim of this study was to examine cardiomyocyte and molecular responses of the right and left ventricle to an acute bout of exhaustive endurance exercise.
Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run. The contractile function and Ca2+ handling properties in isolated cardiomyocytes, protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban including two of its phosphorylated states (serine 16 and threonine 17), and the mitochondrial respiration in permeabilized cardiac muscle fibers were analyzed.
The exercise group showed a significant reduction in cardiomyocyte fractional shortening (right ventricle 1 Hz and 3 Hz p<0.001; left ventricle 1 Hz p<0.05), intracellular Ca2+ amplitude (right ventricle 1 and 3 Hz p<0.001; left ventricle 1 Hz p<0.01 and 3 Hz p<0.05) and rate of diastolic Ca2+ decay (right ventricle 1 Hz p<0.001 and 3 Hz p<0.01; left ventricle 1 and 3 Hz p<0.01). Cardiomyocyte relaxation during diastole was only significantly prolonged at 3 Hz in the right ventricle (p<0.05) compared to sedentary controls. We found an increase in phosphorylation of phospholamban at serine 16 and threonine 17 in the left (p<0.05), but not the right, ventricle from exhaustively exercised animals. The protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban was not changed. Furthermore, we found a reduction in maximal oxidative phosphorylation and electron transport system capacities of mitochondrial respiration in the right (p<0.01 and p<0.05, respectively), but not the left ventricle from rats subjected to acute exhaustive treadmill exercise.
Acute exhaustive treadmill exercise is associated with impairment of cardiomyocyte Ca2+ handling and mitochondrial respiration that causes depression in both contraction and diastolic relaxation of cardiomyocytes.]]></description><subject>Adenosine triphosphatase</subject><subject>Aerobic exercises</subject><subject>Aerobics</subject><subject>Animals</subject><subject>Bioenergetics</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Ca2+-transporting ATPase</subject><subject>Calcium</subject><subject>Calcium (intracellular)</subject><subject>Calcium (mitochondrial)</subject><subject>Calcium (reticular)</subject><subject>Calcium - metabolism</subject><subject>Calcium ions</subject><subject>Cardiac muscle</subject><subject>Cardiology</subject><subject>Cardiomyocytes</subject><subject>Cell Membrane Permeability</subject><subject>Cell Respiration</subject><subject>Cells, Cultured</subject><subject>Contraction</subject><subject>Diastole</subject><subject>Durability</subject><subject>Electron transport</subject><subject>Endurance</subject><subject>Exercise</subject><subject>Fitness equipment</subject><subject>Handling</subject><subject>Heart</subject><subject>Heart - physiopathology</subject><subject>Heart cells</subject><subject>Heart diseases</subject><subject>Ion transport</subject><subject>Male</subject><subject>Medical imaging</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mental depression</subject><subject>Mitochondria</subject><subject>Mitochondria, Heart - metabolism</subject><subject>Muscle contraction</subject><subject>Muscles</subject><subject>Myocardial Contraction</subject><subject>Myocardium - metabolism</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Observations</subject><subject>Oxidative phosphorylation</subject><subject>Phospholamban</subject><subject>Phosphorylation</subject><subject>Physical Conditioning, Animal</subject><subject>Physical fitness</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Properties</subject><subject>Pulmonary arteries</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reduction</subject><subject>Rehabilitation</subject><subject>Research and Analysis Methods</subject><subject>Respiration</subject><subject>Rodents</subject><subject>Running</subject><subject>Sarcoplasmic reticulum</subject><subject>Science</subject><subject>Serine</subject><subject>Studies</subject><subject>Threonine</subject><subject>Ventricle</subject><subject>Working 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exhaustive aerobic exercise training impair cardiomyocyte function and calcium handling in Sprague-Dawley rats</title><author>Ljones, Kristine ; Ness, Henning Ofstad ; Solvang-Garten, Karin ; Gaustad, Svein Erik ; Høydal, Morten Andre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-645d26eed563aabe9add24421f3f35b2d965ce5cfc217e4f48f31fe957d3e74d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenosine triphosphatase</topic><topic>Aerobic exercises</topic><topic>Aerobics</topic><topic>Animals</topic><topic>Bioenergetics</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Ca2+-transporting ATPase</topic><topic>Calcium</topic><topic>Calcium (intracellular)</topic><topic>Calcium (mitochondrial)</topic><topic>Calcium (reticular)</topic><topic>Calcium - metabolism</topic><topic>Calcium ions</topic><topic>Cardiac muscle</topic><topic>Cardiology</topic><topic>Cardiomyocytes</topic><topic>Cell Membrane Permeability</topic><topic>Cell Respiration</topic><topic>Cells, Cultured</topic><topic>Contraction</topic><topic>Diastole</topic><topic>Durability</topic><topic>Electron transport</topic><topic>Endurance</topic><topic>Exercise</topic><topic>Fitness equipment</topic><topic>Handling</topic><topic>Heart</topic><topic>Heart - physiopathology</topic><topic>Heart cells</topic><topic>Heart diseases</topic><topic>Ion transport</topic><topic>Male</topic><topic>Medical imaging</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Mental depression</topic><topic>Mitochondria</topic><topic>Mitochondria, Heart - metabolism</topic><topic>Muscle contraction</topic><topic>Muscles</topic><topic>Myocardial Contraction</topic><topic>Myocardium - metabolism</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Observations</topic><topic>Oxidative 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Erik</au><au>Høydal, Morten Andre</au><au>González, Germán E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute exhaustive aerobic exercise training impair cardiomyocyte function and calcium handling in Sprague-Dawley rats</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-03-08</date><risdate>2017</risdate><volume>12</volume><issue>3</issue><spage>e0173449</spage><epage>e0173449</epage><pages>e0173449-e0173449</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and therefore the aim of this study was to examine cardiomyocyte and molecular responses of the right and left ventricle to an acute bout of exhaustive endurance exercise.
Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run. The contractile function and Ca2+ handling properties in isolated cardiomyocytes, protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban including two of its phosphorylated states (serine 16 and threonine 17), and the mitochondrial respiration in permeabilized cardiac muscle fibers were analyzed.
The exercise group showed a significant reduction in cardiomyocyte fractional shortening (right ventricle 1 Hz and 3 Hz p<0.001; left ventricle 1 Hz p<0.05), intracellular Ca2+ amplitude (right ventricle 1 and 3 Hz p<0.001; left ventricle 1 Hz p<0.01 and 3 Hz p<0.05) and rate of diastolic Ca2+ decay (right ventricle 1 Hz p<0.001 and 3 Hz p<0.01; left ventricle 1 and 3 Hz p<0.01). Cardiomyocyte relaxation during diastole was only significantly prolonged at 3 Hz in the right ventricle (p<0.05) compared to sedentary controls. We found an increase in phosphorylation of phospholamban at serine 16 and threonine 17 in the left (p<0.05), but not the right, ventricle from exhaustively exercised animals. The protein expression levels of sarcoplasmic reticulum Ca2+-ATPase and phospholamban was not changed. Furthermore, we found a reduction in maximal oxidative phosphorylation and electron transport system capacities of mitochondrial respiration in the right (p<0.01 and p<0.05, respectively), but not the left ventricle from rats subjected to acute exhaustive treadmill exercise.
Acute exhaustive treadmill exercise is associated with impairment of cardiomyocyte Ca2+ handling and mitochondrial respiration that causes depression in both contraction and diastolic relaxation of cardiomyocytes.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28273177</pmid><doi>10.1371/journal.pone.0173449</doi><tpages>e0173449</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-03, Vol.12 (3), p.e0173449-e0173449 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1875378293 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adenosine triphosphatase Aerobic exercises Aerobics Animals Bioenergetics Biology and Life Sciences Biomarkers Ca2+-transporting ATPase Calcium Calcium (intracellular) Calcium (mitochondrial) Calcium (reticular) Calcium - metabolism Calcium ions Cardiac muscle Cardiology Cardiomyocytes Cell Membrane Permeability Cell Respiration Cells, Cultured Contraction Diastole Durability Electron transport Endurance Exercise Fitness equipment Handling Heart Heart - physiopathology Heart cells Heart diseases Ion transport Male Medical imaging Medicine Medicine and Health Sciences Mental depression Mitochondria Mitochondria, Heart - metabolism Muscle contraction Muscles Myocardial Contraction Myocardium - metabolism Myocytes, Cardiac - metabolism Observations Oxidative phosphorylation Phospholamban Phosphorylation Physical Conditioning, Animal Physical fitness Physical Sciences Physiological aspects Physiology Properties Pulmonary arteries Rats Rats, Sprague-Dawley Reduction Rehabilitation Research and Analysis Methods Respiration Rodents Running Sarcoplasmic reticulum Science Serine Studies Threonine Ventricle Working groups Workloads |
| title | Acute exhaustive aerobic exercise training impair cardiomyocyte function and calcium handling in Sprague-Dawley rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T19%3A42%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acute%20exhaustive%20aerobic%20exercise%20training%20impair%20cardiomyocyte%20function%20and%20calcium%20handling%20in%20Sprague-Dawley%20rats&rft.jtitle=PloS%20one&rft.au=Ljones,%20Kristine&rft.date=2017-03-08&rft.volume=12&rft.issue=3&rft.spage=e0173449&rft.epage=e0173449&rft.pages=e0173449-e0173449&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0173449&rft_dat=%3Cgale_plos_%3EA484557776%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1875378293&rft_id=info:pmid/28273177&rft_galeid=A484557776&rft_doaj_id=oai_doaj_org_article_c2d49c1066cb44cf9c8c227d5d1a23f2&rfr_iscdi=true |