DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells
Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-03, Vol.12 (3), p.e0173047 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | e0173047 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Liu, Kai Mao, Jian Song, Lipu Fan, Anran Zhang, Sheng Wang, Jianyu Fan, Nana Liu, Na Ye, Xiaoying Fu, Haifeng Zhou, Zhongcheng Wang, Yong Wei, Hong Liu, Zhonghua Li, Ziyi Lai, Liangxue Wang, Xumin Liu, Lin |
| description | Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig iPSCs remain poorly understood. By improving induction conditions using various small chemicals, we generated pig iPSCs that exhibited high pluripotency and differentiation capacity that can contribute to chimeras. However, their potency was reduced with increasing passages by teratoma formation test, and correlated with declined expression levels of Rex1, an important marker for naïve state. By RNA-sequencing analysis, genes related to WNT signaling were upregulated and MAPK signaling and TGFβ pathways downregulated in pig iPSCs compared to fibroblasts, but they were abnormally expressed during passages. Notably, pathways involving in DNA repair and replication were upregulated at early passage, but downregulated in iPSCs during prolonged passage in cluster with fibroblasts. Our data suggests that reduced DNA repair and replication capacity links to the instability of pig iPSCs. Targeting these pathways may facilitate generation of truly pluripotent pig iPSCs, with implication in translational studies. |
| doi_str_mv | 10.1371/journal.pone.0173047 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1873690708</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A483749931</galeid><doaj_id>oai_doaj_org_article_9ae3b31d2282429f98c417255a619ae5</doaj_id><sourcerecordid>A483749931</sourcerecordid><originalsourceid>FETCH-LOGICAL-c791t-c3b46c4f080018185108675b46ee1363cae38c762e8d26dcb22a20b02e0bbf223</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKPxHZuJlXjq9LEEAMusRzHaV1cO9gOov8ep82mBu0C5SLW8XPeY78-J8ueQjCHmMI3G9d7K8y8c1bNAaQYFPRedgorjGYEAXz_aH2SPQphA0CJGSEPsxPEUIlxCU-zH28_LXKvOqF9LmwzLI2WImpnc6Ptz5BHl3em97pzUVm521ONblvllY36QErRCanjLndt3ulVrj9f51IZEx5nD1phgnoy_s-yb-_ffb34OLu8-rC8WFzOJK1gnElcF0QWLWAAQAZZCQEjtExBpSAmWAqFmaQEKdYg0sgaIYFADZACdd0ihM-y5wfdzrjAR2sCh4xiUgEKWCKWB6JxYsM7r7fC77gTmu8Dzq-48FFLo3iVqtUYNijZVKCqrZgsIEVlKQhMe2XSOh-r9fVWNTIZ4YWZiE53rF7zlfvNk-npBXASeDUKePerVyHyrQ6DYcIq1-_PTVNtgEhCX_yD3n27kVqJdAFtW5fqykGULwqGaVFVGCZqfgeVvkZttUx91OoUnyS8niQkJqo_cSX6EPjy-sv_s1ffp-zLI3athInr4Ew_NFOYgsUBlN6F4FV7azIEfBiDGzf4MAZ8HIOU9uz4gW6Tbvoe_wXNUgET</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1873690708</pqid></control><display><type>article</type><title>DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Liu, Kai ; Mao, Jian ; Song, Lipu ; Fan, Anran ; Zhang, Sheng ; Wang, Jianyu ; Fan, Nana ; Liu, Na ; Ye, Xiaoying ; Fu, Haifeng ; Zhou, Zhongcheng ; Wang, Yong ; Wei, Hong ; Liu, Zhonghua ; Li, Ziyi ; Lai, Liangxue ; Wang, Xumin ; Liu, Lin</creator><contributor>Cooney, Austin John</contributor><creatorcontrib>Liu, Kai ; Mao, Jian ; Song, Lipu ; Fan, Anran ; Zhang, Sheng ; Wang, Jianyu ; Fan, Nana ; Liu, Na ; Ye, Xiaoying ; Fu, Haifeng ; Zhou, Zhongcheng ; Wang, Yong ; Wei, Hong ; Liu, Zhonghua ; Li, Ziyi ; Lai, Liangxue ; Wang, Xumin ; Liu, Lin ; Cooney, Austin John</creatorcontrib><description>Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig iPSCs remain poorly understood. By improving induction conditions using various small chemicals, we generated pig iPSCs that exhibited high pluripotency and differentiation capacity that can contribute to chimeras. However, their potency was reduced with increasing passages by teratoma formation test, and correlated with declined expression levels of Rex1, an important marker for naïve state. By RNA-sequencing analysis, genes related to WNT signaling were upregulated and MAPK signaling and TGFβ pathways downregulated in pig iPSCs compared to fibroblasts, but they were abnormally expressed during passages. Notably, pathways involving in DNA repair and replication were upregulated at early passage, but downregulated in iPSCs during prolonged passage in cluster with fibroblasts. Our data suggests that reduced DNA repair and replication capacity links to the instability of pig iPSCs. Targeting these pathways may facilitate generation of truly pluripotent pig iPSCs, with implication in translational studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0173047</identifier><identifier>PMID: 28253351</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal models ; Animals ; Biology ; Biology and Life Sciences ; Cell Differentiation ; Cell lines ; Chimeras ; Collaboration ; Deoxyribonucleic acid ; Differentiation ; DNA ; DNA biosynthesis ; DNA methylation ; DNA Repair ; DNA Replication ; Embryonic stem cells ; Epigenetics ; Fibroblasts ; Gene expression ; Gene sequencing ; Genomics ; Health aspects ; Hogs ; Hospitals ; Induced Pluripotent Stem Cells - cytology ; Laboratories ; Life sciences ; MAP kinase ; MAP Kinase Signaling System ; Medicine and Health Sciences ; Mice ; Physical Sciences ; Pigs ; Pluripotency ; Quality ; Repair ; Replication ; Ribonucleic acid ; RNA ; Signal transduction ; Signaling ; Stability ; Stem cell transplantation ; Stem cells ; Swine ; Teratoma ; Wnt protein</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0173047</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Liu et al 2017 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-c3b46c4f080018185108675b46ee1363cae38c762e8d26dcb22a20b02e0bbf223</citedby><cites>FETCH-LOGICAL-c791t-c3b46c4f080018185108675b46ee1363cae38c762e8d26dcb22a20b02e0bbf223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333863/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333863/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28253351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Cooney, Austin John</contributor><creatorcontrib>Liu, Kai</creatorcontrib><creatorcontrib>Mao, Jian</creatorcontrib><creatorcontrib>Song, Lipu</creatorcontrib><creatorcontrib>Fan, Anran</creatorcontrib><creatorcontrib>Zhang, Sheng</creatorcontrib><creatorcontrib>Wang, Jianyu</creatorcontrib><creatorcontrib>Fan, Nana</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Ye, Xiaoying</creatorcontrib><creatorcontrib>Fu, Haifeng</creatorcontrib><creatorcontrib>Zhou, Zhongcheng</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Wei, Hong</creatorcontrib><creatorcontrib>Liu, Zhonghua</creatorcontrib><creatorcontrib>Li, Ziyi</creatorcontrib><creatorcontrib>Lai, Liangxue</creatorcontrib><creatorcontrib>Wang, Xumin</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><title>DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig iPSCs remain poorly understood. By improving induction conditions using various small chemicals, we generated pig iPSCs that exhibited high pluripotency and differentiation capacity that can contribute to chimeras. However, their potency was reduced with increasing passages by teratoma formation test, and correlated with declined expression levels of Rex1, an important marker for naïve state. By RNA-sequencing analysis, genes related to WNT signaling were upregulated and MAPK signaling and TGFβ pathways downregulated in pig iPSCs compared to fibroblasts, but they were abnormally expressed during passages. Notably, pathways involving in DNA repair and replication were upregulated at early passage, but downregulated in iPSCs during prolonged passage in cluster with fibroblasts. Our data suggests that reduced DNA repair and replication capacity links to the instability of pig iPSCs. Targeting these pathways may facilitate generation of truly pluripotent pig iPSCs, with implication in translational studies.</description><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Cell Differentiation</subject><subject>Cell lines</subject><subject>Chimeras</subject><subject>Collaboration</subject><subject>Deoxyribonucleic acid</subject><subject>Differentiation</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA methylation</subject><subject>DNA Repair</subject><subject>DNA Replication</subject><subject>Embryonic stem cells</subject><subject>Epigenetics</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Hogs</subject><subject>Hospitals</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>MAP kinase</subject><subject>MAP Kinase Signaling System</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Physical Sciences</subject><subject>Pigs</subject><subject>Pluripotency</subject><subject>Quality</subject><subject>Repair</subject><subject>Replication</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Stability</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Swine</subject><subject>Teratoma</subject><subject>Wnt protein</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKPxHZuJlXjq9LEEAMusRzHaV1cO9gOov8ep82mBu0C5SLW8XPeY78-J8ueQjCHmMI3G9d7K8y8c1bNAaQYFPRedgorjGYEAXz_aH2SPQphA0CJGSEPsxPEUIlxCU-zH28_LXKvOqF9LmwzLI2WImpnc6Ptz5BHl3em97pzUVm521ONblvllY36QErRCanjLndt3ulVrj9f51IZEx5nD1phgnoy_s-yb-_ffb34OLu8-rC8WFzOJK1gnElcF0QWLWAAQAZZCQEjtExBpSAmWAqFmaQEKdYg0sgaIYFADZACdd0ihM-y5wfdzrjAR2sCh4xiUgEKWCKWB6JxYsM7r7fC77gTmu8Dzq-48FFLo3iVqtUYNijZVKCqrZgsIEVlKQhMe2XSOh-r9fVWNTIZ4YWZiE53rF7zlfvNk-npBXASeDUKePerVyHyrQ6DYcIq1-_PTVNtgEhCX_yD3n27kVqJdAFtW5fqykGULwqGaVFVGCZqfgeVvkZttUx91OoUnyS8niQkJqo_cSX6EPjy-sv_s1ffp-zLI3athInr4Ew_NFOYgsUBlN6F4FV7azIEfBiDGzf4MAZ8HIOU9uz4gW6Tbvoe_wXNUgET</recordid><startdate>20170302</startdate><enddate>20170302</enddate><creator>Liu, Kai</creator><creator>Mao, Jian</creator><creator>Song, Lipu</creator><creator>Fan, Anran</creator><creator>Zhang, Sheng</creator><creator>Wang, Jianyu</creator><creator>Fan, Nana</creator><creator>Liu, Na</creator><creator>Ye, Xiaoying</creator><creator>Fu, Haifeng</creator><creator>Zhou, Zhongcheng</creator><creator>Wang, Yong</creator><creator>Wei, Hong</creator><creator>Liu, Zhonghua</creator><creator>Li, Ziyi</creator><creator>Lai, Liangxue</creator><creator>Wang, Xumin</creator><creator>Liu, Lin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170302</creationdate><title>DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells</title><author>Liu, Kai ; Mao, Jian ; Song, Lipu ; Fan, Anran ; Zhang, Sheng ; Wang, Jianyu ; Fan, Nana ; Liu, Na ; Ye, Xiaoying ; Fu, Haifeng ; Zhou, Zhongcheng ; Wang, Yong ; Wei, Hong ; Liu, Zhonghua ; Li, Ziyi ; Lai, Liangxue ; Wang, Xumin ; Liu, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-c3b46c4f080018185108675b46ee1363cae38c762e8d26dcb22a20b02e0bbf223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Animal models</topic><topic>Animals</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Cell Differentiation</topic><topic>Cell lines</topic><topic>Chimeras</topic><topic>Collaboration</topic><topic>Deoxyribonucleic acid</topic><topic>Differentiation</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA methylation</topic><topic>DNA Repair</topic><topic>DNA Replication</topic><topic>Embryonic stem cells</topic><topic>Epigenetics</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Hogs</topic><topic>Hospitals</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Laboratories</topic><topic>Life sciences</topic><topic>MAP kinase</topic><topic>MAP Kinase Signaling System</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Physical Sciences</topic><topic>Pigs</topic><topic>Pluripotency</topic><topic>Quality</topic><topic>Repair</topic><topic>Replication</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Stability</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Swine</topic><topic>Teratoma</topic><topic>Wnt protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Kai</creatorcontrib><creatorcontrib>Mao, Jian</creatorcontrib><creatorcontrib>Song, Lipu</creatorcontrib><creatorcontrib>Fan, Anran</creatorcontrib><creatorcontrib>Zhang, Sheng</creatorcontrib><creatorcontrib>Wang, Jianyu</creatorcontrib><creatorcontrib>Fan, Nana</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Ye, Xiaoying</creatorcontrib><creatorcontrib>Fu, Haifeng</creatorcontrib><creatorcontrib>Zhou, Zhongcheng</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Wei, Hong</creatorcontrib><creatorcontrib>Liu, Zhonghua</creatorcontrib><creatorcontrib>Li, Ziyi</creatorcontrib><creatorcontrib>Lai, Liangxue</creatorcontrib><creatorcontrib>Wang, Xumin</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Kai</au><au>Mao, Jian</au><au>Song, Lipu</au><au>Fan, Anran</au><au>Zhang, Sheng</au><au>Wang, Jianyu</au><au>Fan, Nana</au><au>Liu, Na</au><au>Ye, Xiaoying</au><au>Fu, Haifeng</au><au>Zhou, Zhongcheng</au><au>Wang, Yong</au><au>Wei, Hong</au><au>Liu, Zhonghua</au><au>Li, Ziyi</au><au>Lai, Liangxue</au><au>Wang, Xumin</au><au>Liu, Lin</au><au>Cooney, Austin John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-03-02</date><risdate>2017</risdate><volume>12</volume><issue>3</issue><spage>e0173047</spage><pages>e0173047-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pigs are proposed to be suitable large animal models for test of the efficacy and safety of induced pluripotent stem cells (iPSCs) for stem cell therapy, but authentic pig ES/iPS cell lines with germline competence are rarely produced. The pathways or signaling underlying the defective competent pig iPSCs remain poorly understood. By improving induction conditions using various small chemicals, we generated pig iPSCs that exhibited high pluripotency and differentiation capacity that can contribute to chimeras. However, their potency was reduced with increasing passages by teratoma formation test, and correlated with declined expression levels of Rex1, an important marker for naïve state. By RNA-sequencing analysis, genes related to WNT signaling were upregulated and MAPK signaling and TGFβ pathways downregulated in pig iPSCs compared to fibroblasts, but they were abnormally expressed during passages. Notably, pathways involving in DNA repair and replication were upregulated at early passage, but downregulated in iPSCs during prolonged passage in cluster with fibroblasts. Our data suggests that reduced DNA repair and replication capacity links to the instability of pig iPSCs. Targeting these pathways may facilitate generation of truly pluripotent pig iPSCs, with implication in translational studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28253351</pmid><doi>10.1371/journal.pone.0173047</doi><tpages>e0173047</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-03, Vol.12 (3), p.e0173047 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1873690708 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal models Animals Biology Biology and Life Sciences Cell Differentiation Cell lines Chimeras Collaboration Deoxyribonucleic acid Differentiation DNA DNA biosynthesis DNA methylation DNA Repair DNA Replication Embryonic stem cells Epigenetics Fibroblasts Gene expression Gene sequencing Genomics Health aspects Hogs Hospitals Induced Pluripotent Stem Cells - cytology Laboratories Life sciences MAP kinase MAP Kinase Signaling System Medicine and Health Sciences Mice Physical Sciences Pigs Pluripotency Quality Repair Replication Ribonucleic acid RNA Signal transduction Signaling Stability Stem cell transplantation Stem cells Swine Teratoma Wnt protein |
| title | DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T09%3A05%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20repair%20and%20replication%20links%20to%20pluripotency%20and%20differentiation%20capacity%20of%20pig%20iPS%20cells&rft.jtitle=PloS%20one&rft.au=Liu,%20Kai&rft.date=2017-03-02&rft.volume=12&rft.issue=3&rft.spage=e0173047&rft.pages=e0173047-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0173047&rft_dat=%3Cgale_plos_%3EA483749931%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1873690708&rft_id=info:pmid/28253351&rft_galeid=A483749931&rft_doaj_id=oai_doaj_org_article_9ae3b31d2282429f98c417255a619ae5&rfr_iscdi=true |