A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae
Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus...
Gespeichert in:
| Veröffentlicht in: | PloS one 2017-03, Vol.12 (3), p.e0173059-e0173059 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0173059 |
|---|---|
| container_issue | 3 |
| container_start_page | e0173059 |
| container_title | PloS one |
| container_volume | 12 |
| creator | Nakayama, Darlan Gonçalves Santos Júnior, Célio Dias Kishi, Luciano Takeshi Pedezzi, Rafael Santiago, Adelita Carolina Soares-Costa, Andrea Henrique-Silva, Flavio |
| description | Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus, a cerambycidae beetle) is known to cause severe damage to the crops in Brazil. The absence of molecular information about this insect reinforces the need for studies and an effective method to control this pest. In this study, RNA-Seq technology was employed to study different parts of M. fryanus larvae. The generated data will help in further investigations about the taxonomy, development, and adaptation of this insect. RNA was extracted from six different parts (head, fat body, integument, hindgut, midgut, and foregut) using Trizol methodology. Using Illumina paired-end sequencing technology and the Trinity platform, trimming and de novo assembly was performed, resulting in 44,567 contigs longer than 200 nt for a reunion of data from all transcriptomes, with a mean length of 1,095.27 nt. Transcripts were annotated using BLAST against different protein databanks (Uniprot/Swissprot, PFAM, KEEG, SignalP 4.1, Gene Ontology, and CAZY) and were compared for similarity using a Venn diagram. Differential expression patterns were studied for select genes through qPCR and FPKM comprising important protein families (digestive peptidases, glucosyl hydrolases, serine protease inhibitors and otopetrin), which allowed a better understanding of the insect's digestion, immunity and gravity sensorial mechanisms. |
| doi_str_mv | 10.1371/journal.pone.0173059 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1873345184</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A483676009</galeid><doaj_id>oai_doaj_org_article_894e1073a58a44448630a2ca6c93fd54</doaj_id><sourcerecordid>A483676009</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-ca923ae2866bd74eeb7f9c4af303760c0ccea77896198a8563764c7bd070a9f33</originalsourceid><addsrcrecordid>eNqNk1tr2zAUx83YWC_bNxibYTDah2S62ZJeBqHsEsgo7PYqTmTZUXCsVLLD0k8_uXFLPPpQ-0Hi-Hf-Ov7rnCR5g9EUU44_rl3nG6inW9eYKcKcokw-S06xpGSSE0SfH-1PkrMQ1ghlVOT5y-SECMKElOg0WczS1kMTtLfb1m2sTkPnd2afujL9bqvC1V1IS7-HJq4XoavAa2hM6lf21m1MunTe-Mu0Br8D8yp5UUIdzOthPU9-f_n86-rbZHH9dX41W0w0J1k70SAJBUNiLcuCM2OWvJSaQUkR5TnSSGsDnAuZYylAZHmMMs2XBeIIZEnpefLuoLutXVCDEUFhwSllGRYsEvMDUThYq623G_B75cCqu4DzlQLfWl0bJSQzGHEKmQAWH5FTBERDriUti6zX-jSc1i03ptCmiY7VI9Hxl8auVOV2KqOUYNSXezEIeHfTmdCqjQ3a1HU00nV3dXNBiBDyKSjlhOUCRfT9f-jjRgxUBfFfbVO6WKLuRdWMCZpHv1F_7PQRKr6FiS0RG6y0MT5KuBwlRKY1f9sKuhDU_OePp7PXf8bshyN2ZaBuVyH2YGtdE8YgO4DauxC8KR_uAyPVz8e9G6qfDzXMR0x7e3yXD0n3A0H_AaikCSs</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1873345184</pqid></control><display><type>article</type><title>A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae</title><source>Open Access: DOAJ - Directory of Open Access Journals</source><source>MEDLINE</source><source>Public Library of Science（OA）</source><source>PubMed Central（OA）</source><source>Free E-Journal (出版社公開部分のみ）</source><source>Free Full-Text Journals in Chemistry</source><creator>Nakayama, Darlan Gonçalves ; Santos Júnior, Célio Dias ; Kishi, Luciano Takeshi ; Pedezzi, Rafael ; Santiago, Adelita Carolina ; Soares-Costa, Andrea ; Henrique-Silva, Flavio</creator><contributor>Doucet, Daniel</contributor><creatorcontrib>Nakayama, Darlan Gonçalves ; Santos Júnior, Célio Dias ; Kishi, Luciano Takeshi ; Pedezzi, Rafael ; Santiago, Adelita Carolina ; Soares-Costa, Andrea ; Henrique-Silva, Flavio ; Doucet, Daniel</creatorcontrib><description>Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus, a cerambycidae beetle) is known to cause severe damage to the crops in Brazil. The absence of molecular information about this insect reinforces the need for studies and an effective method to control this pest. In this study, RNA-Seq technology was employed to study different parts of M. fryanus larvae. The generated data will help in further investigations about the taxonomy, development, and adaptation of this insect. RNA was extracted from six different parts (head, fat body, integument, hindgut, midgut, and foregut) using Trizol methodology. Using Illumina paired-end sequencing technology and the Trinity platform, trimming and de novo assembly was performed, resulting in 44,567 contigs longer than 200 nt for a reunion of data from all transcriptomes, with a mean length of 1,095.27 nt. Transcripts were annotated using BLAST against different protein databanks (Uniprot/Swissprot, PFAM, KEEG, SignalP 4.1, Gene Ontology, and CAZY) and were compared for similarity using a Venn diagram. Differential expression patterns were studied for select genes through qPCR and FPKM comprising important protein families (digestive peptidases, glucosyl hydrolases, serine protease inhibitors and otopetrin), which allowed a better understanding of the insect's digestion, immunity and gravity sensorial mechanisms.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0173059</identifier><identifier>PMID: 28248990</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biofuels ; Biology and Life Sciences ; Borers ; Cerambycidae ; Cloning ; Coleoptera ; Coleoptera - genetics ; Coleoptera - growth & development ; Coleoptera - metabolism ; Coleoptera - pathogenicity ; Crop damage ; Crops ; Data banks ; Ethanol ; Evolution ; Fat body ; Foregut ; Gene expression ; Gravitation ; Gravity ; Hindgut ; Immunity ; Insect pests ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insects ; Integument ; Kinases ; Laboratories ; Larvae ; Medicine and Health Sciences ; Midgut ; Molecular biology ; Molecular Sequence Annotation ; Open Reading Frames ; Peptidases ; Pest control ; Pests ; Physical Sciences ; Protease inhibitors ; Protein families ; Proteinase inhibitors ; Ribonucleic acid ; RNA ; RNA sequencing ; Saccharum - parasitology ; Saccharum officinarum ; Serine ; Serine proteinase ; Studies ; Sucrose ; Sugar ; Sugarcane ; Taxonomy ; Technology ; Transcription (Genetics) ; Transcriptome ; Tribolium ; Venn diagrams ; Zebrafish</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0173059-e0173059</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Nakayama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Nakayama et al 2017 Nakayama et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-ca923ae2866bd74eeb7f9c4af303760c0ccea77896198a8563764c7bd070a9f33</citedby><cites>FETCH-LOGICAL-c725t-ca923ae2866bd74eeb7f9c4af303760c0ccea77896198a8563764c7bd070a9f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332103/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332103/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28248990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Doucet, Daniel</contributor><creatorcontrib>Nakayama, Darlan Gonçalves</creatorcontrib><creatorcontrib>Santos Júnior, Célio Dias</creatorcontrib><creatorcontrib>Kishi, Luciano Takeshi</creatorcontrib><creatorcontrib>Pedezzi, Rafael</creatorcontrib><creatorcontrib>Santiago, Adelita Carolina</creatorcontrib><creatorcontrib>Soares-Costa, Andrea</creatorcontrib><creatorcontrib>Henrique-Silva, Flavio</creatorcontrib><title>A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus, a cerambycidae beetle) is known to cause severe damage to the crops in Brazil. The absence of molecular information about this insect reinforces the need for studies and an effective method to control this pest. In this study, RNA-Seq technology was employed to study different parts of M. fryanus larvae. The generated data will help in further investigations about the taxonomy, development, and adaptation of this insect. RNA was extracted from six different parts (head, fat body, integument, hindgut, midgut, and foregut) using Trizol methodology. Using Illumina paired-end sequencing technology and the Trinity platform, trimming and de novo assembly was performed, resulting in 44,567 contigs longer than 200 nt for a reunion of data from all transcriptomes, with a mean length of 1,095.27 nt. Transcripts were annotated using BLAST against different protein databanks (Uniprot/Swissprot, PFAM, KEEG, SignalP 4.1, Gene Ontology, and CAZY) and were compared for similarity using a Venn diagram. Differential expression patterns were studied for select genes through qPCR and FPKM comprising important protein families (digestive peptidases, glucosyl hydrolases, serine protease inhibitors and otopetrin), which allowed a better understanding of the insect's digestion, immunity and gravity sensorial mechanisms.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biofuels</subject><subject>Biology and Life Sciences</subject><subject>Borers</subject><subject>Cerambycidae</subject><subject>Cloning</subject><subject>Coleoptera</subject><subject>Coleoptera - genetics</subject><subject>Coleoptera - growth & development</subject><subject>Coleoptera - metabolism</subject><subject>Coleoptera - pathogenicity</subject><subject>Crop damage</subject><subject>Crops</subject><subject>Data banks</subject><subject>Ethanol</subject><subject>Evolution</subject><subject>Fat body</subject><subject>Foregut</subject><subject>Gene expression</subject><subject>Gravitation</subject><subject>Gravity</subject><subject>Hindgut</subject><subject>Immunity</subject><subject>Insect pests</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insects</subject><subject>Integument</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Larvae</subject><subject>Medicine and Health Sciences</subject><subject>Midgut</subject><subject>Molecular biology</subject><subject>Molecular Sequence Annotation</subject><subject>Open Reading Frames</subject><subject>Peptidases</subject><subject>Pest control</subject><subject>Pests</subject><subject>Physical Sciences</subject><subject>Protease inhibitors</subject><subject>Protein families</subject><subject>Proteinase inhibitors</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>Saccharum - parasitology</subject><subject>Saccharum officinarum</subject><subject>Serine</subject><subject>Serine proteinase</subject><subject>Studies</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Sugarcane</subject><subject>Taxonomy</subject><subject>Technology</subject><subject>Transcription (Genetics)</subject><subject>Transcriptome</subject><subject>Tribolium</subject><subject>Venn diagrams</subject><subject>Zebrafish</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1tr2zAUx83YWC_bNxibYTDah2S62ZJeBqHsEsgo7PYqTmTZUXCsVLLD0k8_uXFLPPpQ-0Hi-Hf-Ov7rnCR5g9EUU44_rl3nG6inW9eYKcKcokw-S06xpGSSE0SfH-1PkrMQ1ghlVOT5y-SECMKElOg0WczS1kMTtLfb1m2sTkPnd2afujL9bqvC1V1IS7-HJq4XoavAa2hM6lf21m1MunTe-Mu0Br8D8yp5UUIdzOthPU9-f_n86-rbZHH9dX41W0w0J1k70SAJBUNiLcuCM2OWvJSaQUkR5TnSSGsDnAuZYylAZHmMMs2XBeIIZEnpefLuoLutXVCDEUFhwSllGRYsEvMDUThYq623G_B75cCqu4DzlQLfWl0bJSQzGHEKmQAWH5FTBERDriUti6zX-jSc1i03ptCmiY7VI9Hxl8auVOV2KqOUYNSXezEIeHfTmdCqjQ3a1HU00nV3dXNBiBDyKSjlhOUCRfT9f-jjRgxUBfFfbVO6WKLuRdWMCZpHv1F_7PQRKr6FiS0RG6y0MT5KuBwlRKY1f9sKuhDU_OePp7PXf8bshyN2ZaBuVyH2YGtdE8YgO4DauxC8KR_uAyPVz8e9G6qfDzXMR0x7e3yXD0n3A0H_AaikCSs</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Nakayama, Darlan Gonçalves</creator><creator>Santos Júnior, Célio Dias</creator><creator>Kishi, Luciano Takeshi</creator><creator>Pedezzi, Rafael</creator><creator>Santiago, Adelita Carolina</creator><creator>Soares-Costa, Andrea</creator><creator>Henrique-Silva, Flavio</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170301</creationdate><title>A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae</title><author>Nakayama, Darlan Gonçalves ; Santos Júnior, Célio Dias ; Kishi, Luciano Takeshi ; Pedezzi, Rafael ; Santiago, Adelita Carolina ; Soares-Costa, Andrea ; Henrique-Silva, Flavio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-ca923ae2866bd74eeb7f9c4af303760c0ccea77896198a8563764c7bd070a9f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biofuels</topic><topic>Biology and Life Sciences</topic><topic>Borers</topic><topic>Cerambycidae</topic><topic>Cloning</topic><topic>Coleoptera</topic><topic>Coleoptera - genetics</topic><topic>Coleoptera - growth & development</topic><topic>Coleoptera - metabolism</topic><topic>Coleoptera - pathogenicity</topic><topic>Crop damage</topic><topic>Crops</topic><topic>Data banks</topic><topic>Ethanol</topic><topic>Evolution</topic><topic>Fat body</topic><topic>Foregut</topic><topic>Gene expression</topic><topic>Gravitation</topic><topic>Gravity</topic><topic>Hindgut</topic><topic>Immunity</topic><topic>Insect pests</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insects</topic><topic>Integument</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Larvae</topic><topic>Medicine and Health Sciences</topic><topic>Midgut</topic><topic>Molecular biology</topic><topic>Molecular Sequence Annotation</topic><topic>Open Reading Frames</topic><topic>Peptidases</topic><topic>Pest control</topic><topic>Pests</topic><topic>Physical Sciences</topic><topic>Protease inhibitors</topic><topic>Protein families</topic><topic>Proteinase inhibitors</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>Saccharum - parasitology</topic><topic>Saccharum officinarum</topic><topic>Serine</topic><topic>Serine proteinase</topic><topic>Studies</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Sugarcane</topic><topic>Taxonomy</topic><topic>Technology</topic><topic>Transcription (Genetics)</topic><topic>Transcriptome</topic><topic>Tribolium</topic><topic>Venn diagrams</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakayama, Darlan Gonçalves</creatorcontrib><creatorcontrib>Santos Júnior, Célio Dias</creatorcontrib><creatorcontrib>Kishi, Luciano Takeshi</creatorcontrib><creatorcontrib>Pedezzi, Rafael</creatorcontrib><creatorcontrib>Santiago, Adelita Carolina</creatorcontrib><creatorcontrib>Soares-Costa, Andrea</creatorcontrib><creatorcontrib>Henrique-Silva, Flavio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakayama, Darlan Gonçalves</au><au>Santos Júnior, Célio Dias</au><au>Kishi, Luciano Takeshi</au><au>Pedezzi, Rafael</au><au>Santiago, Adelita Carolina</au><au>Soares-Costa, Andrea</au><au>Henrique-Silva, Flavio</au><au>Doucet, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-03-01</date><risdate>2017</risdate><volume>12</volume><issue>3</issue><spage>e0173059</spage><epage>e0173059</epage><pages>e0173059-e0173059</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Sugarcane, a major crop grown in the tropical and subtropical areas of the world, is produced mainly for sucrose, which is used as a sweetener or for the production of bioethanol. Among the numerous pests that significantly affect the yield of sugarcane, the sugarcane rhizome borer (Migdolus fryanus, a cerambycidae beetle) is known to cause severe damage to the crops in Brazil. The absence of molecular information about this insect reinforces the need for studies and an effective method to control this pest. In this study, RNA-Seq technology was employed to study different parts of M. fryanus larvae. The generated data will help in further investigations about the taxonomy, development, and adaptation of this insect. RNA was extracted from six different parts (head, fat body, integument, hindgut, midgut, and foregut) using Trizol methodology. Using Illumina paired-end sequencing technology and the Trinity platform, trimming and de novo assembly was performed, resulting in 44,567 contigs longer than 200 nt for a reunion of data from all transcriptomes, with a mean length of 1,095.27 nt. Transcripts were annotated using BLAST against different protein databanks (Uniprot/Swissprot, PFAM, KEEG, SignalP 4.1, Gene Ontology, and CAZY) and were compared for similarity using a Venn diagram. Differential expression patterns were studied for select genes through qPCR and FPKM comprising important protein families (digestive peptidases, glucosyl hydrolases, serine protease inhibitors and otopetrin), which allowed a better understanding of the insect's digestion, immunity and gravity sensorial mechanisms.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28248990</pmid><doi>10.1371/journal.pone.0173059</doi><tpages>e0173059</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-03, Vol.12 (3), p.e0173059-e0173059 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1873345184 |
| source | Open Access: DOAJ - Directory of Open Access Journals; MEDLINE; Public Library of Science（OA）; PubMed Central（OA）; Free E-Journal (出版社公開部分のみ）; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Biofuels Biology and Life Sciences Borers Cerambycidae Cloning Coleoptera Coleoptera - genetics Coleoptera - growth & development Coleoptera - metabolism Coleoptera - pathogenicity Crop damage Crops Data banks Ethanol Evolution Fat body Foregut Gene expression Gravitation Gravity Hindgut Immunity Insect pests Insect Proteins - genetics Insect Proteins - metabolism Insects Integument Kinases Laboratories Larvae Medicine and Health Sciences Midgut Molecular biology Molecular Sequence Annotation Open Reading Frames Peptidases Pest control Pests Physical Sciences Protease inhibitors Protein families Proteinase inhibitors Ribonucleic acid RNA RNA sequencing Saccharum - parasitology Saccharum officinarum Serine Serine proteinase Studies Sucrose Sugar Sugarcane Taxonomy Technology Transcription (Genetics) Transcriptome Tribolium Venn diagrams Zebrafish |
| title | A transcriptomic survey of Migdolus fryanus (sugarcane rhizome borer) larvae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T08%3A20%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20transcriptomic%20survey%20of%20Migdolus%20fryanus%20(sugarcane%20rhizome%20borer)%20larvae&rft.jtitle=PloS%20one&rft.au=Nakayama,%20Darlan%20Gon%C3%A7alves&rft.date=2017-03-01&rft.volume=12&rft.issue=3&rft.spage=e0173059&rft.epage=e0173059&rft.pages=e0173059-e0173059&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0173059&rft_dat=%3Cgale_plos_%3EA483676009%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1873345184&rft_id=info:pmid/28248990&rft_galeid=A483676009&rft_doaj_id=oai_doaj_org_article_894e1073a58a44448630a2ca6c93fd54&rfr_iscdi=true |