Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system
The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent d...
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| description | The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury. |
| doi_str_mv | 10.1371/journal.pone.0172736 |
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The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0172736</identifier><identifier>PMID: 28234971</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Axon guidance ; Axons ; Axons - metabolism ; Biology and Life Sciences ; Blood vessels ; Blood Vessels - metabolism ; Brain ; Cell adhesion & migration ; Cell migration ; Cell Movement - genetics ; Cells (biology) ; Dentistry ; Dorsal root ganglia ; Drosophila ; Fibroblasts ; Ganglia, Spinal - metabolism ; Gene expression ; Gene Expression Regulation, Developmental ; Glycoproteins ; Homeostasis ; Immunoglobulins ; Insects ; Intercellular Signaling Peptides and Proteins - biosynthesis ; Intercellular Signaling Peptides and Proteins - metabolism ; Mammals ; Medicine and Health Sciences ; Membrane Proteins - biosynthesis ; Membrane Proteins - metabolism ; Mice ; Motor neurons ; Motor Neurons - metabolism ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Nervous system ; Neurons ; Peripheral nerves ; Peripheral nervous system ; Peripheral Nervous System - metabolism ; Phosphorylation ; Physiological aspects ; Proteins ; Receptors ; Receptors, Immunologic - biosynthesis ; Receptors, Immunologic - metabolism ; Research and Analysis Methods ; Rodents ; Roundabout Proteins ; Satellite cells ; Schwann cells ; Sciatic nerve ; Sciatic Nerve - metabolism ; Sensory neurons ; Sensory Receptor Cells - metabolism ; Sensory receptors ; Slit protein ; Spinal cord ; Spinal Cord - metabolism</subject><ispartof>PloS one, 2017-02, Vol.12 (2), p.e0172736-e0172736</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Carr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Carr et al 2017 Carr et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-7d011f91e5de8a5fb03aca4cdc54d406b72b950d524321af136e05fa125b9ab23</citedby><cites>FETCH-LOGICAL-c725t-7d011f91e5de8a5fb03aca4cdc54d406b72b950d524321af136e05fa125b9ab23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325304/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325304/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28234971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gillingwater, Thomas H</contributor><creatorcontrib>Carr, Lauren</creatorcontrib><creatorcontrib>Parkinson, David B</creatorcontrib><creatorcontrib>Dun, Xin-Peng</creatorcontrib><title>Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury.</description><subject>Animals</subject><subject>Axon guidance</subject><subject>Axons</subject><subject>Axons - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Blood vessels</subject><subject>Blood Vessels - metabolism</subject><subject>Brain</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cells (biology)</subject><subject>Dentistry</subject><subject>Dorsal root ganglia</subject><subject>Drosophila</subject><subject>Fibroblasts</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Glycoproteins</subject><subject>Homeostasis</subject><subject>Immunoglobulins</subject><subject>Insects</subject><subject>Intercellular Signaling Peptides and Proteins - 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metabolism</subject><subject>Sensory neurons</subject><subject>Sensory Receptor Cells - metabolism</subject><subject>Sensory receptors</subject><subject>Slit protein</subject><subject>Spinal cord</subject><subject>Spinal Cord - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIHqxaz7n40YopepCodCqtyGTObObZWYyTTKl--_NdKdlR3pRcpFw8rxvTk5ykuQ9wUvCMvJtawfXqWbZ2w6WmGQ0Y-mL5JgUjC5SitnLg_VR8sb7LcaC5Wn6OjmiOWW8yMhx0pzf9Q68N7ZDvQoBXOeRrdF1YwJSXYWubGlRrVrT7FALbQnOI9MhVQ1NQK0dPCDfm5gI0tZV95IenOk34GKsA3cbGeR3PkD7NnlVq8bDu2k-Sf78OP999mtxcflzdXZ6sdAZFWGRVZiQuiAgKsiVqEvMlFZcV1rwiuO0zGhZCFwJyhklqiYsBSxqRagoC1VSdpJ83Pv2jfVyKpSXJM9IjhnmIhKrPVFZtZW9M61yO2mVkfcB69ZSuWB0AzKlSpBC5DkIzbVgSmGiMwI1L1TG6Oj1fTptKFuoNHQhXn1mOt_pzEau7a0UoxrzaPBlMnD2ZgAfZGu8hqZRHcTijXlnucCkKJ6DUpFxnrOIfvoPfboQE7VW8a6mq21MUY-m8pTnVHAsCI7U8gkqjgpao-MHrE2MzwRfZ4LIBLgLazV4L1fXV89nL__O2c8H7AZUEzbeNkOI_9fPQb4HtbPeO6gf34NgOfbPQzXk2D9y6p8o-3D4lo-ih4Zh_wCR0BUk</recordid><startdate>20170224</startdate><enddate>20170224</enddate><creator>Carr, Lauren</creator><creator>Parkinson, David B</creator><creator>Dun, Xin-Peng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170224</creationdate><title>Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system</title><author>Carr, Lauren ; Parkinson, David B ; Dun, Xin-Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-7d011f91e5de8a5fb03aca4cdc54d406b72b950d524321af136e05fa125b9ab23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Axon guidance</topic><topic>Axons</topic><topic>Axons - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Blood vessels</topic><topic>Blood Vessels - metabolism</topic><topic>Brain</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cells (biology)</topic><topic>Dentistry</topic><topic>Dorsal root ganglia</topic><topic>Drosophila</topic><topic>Fibroblasts</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Glycoproteins</topic><topic>Homeostasis</topic><topic>Immunoglobulins</topic><topic>Insects</topic><topic>Intercellular Signaling Peptides and Proteins - biosynthesis</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Mammals</topic><topic>Medicine and Health Sciences</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Motor neurons</topic><topic>Motor Neurons - metabolism</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Nervous system</topic><topic>Neurons</topic><topic>Peripheral nerves</topic><topic>Peripheral nervous system</topic><topic>Peripheral Nervous System - metabolism</topic><topic>Phosphorylation</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Immunologic - biosynthesis</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Roundabout Proteins</topic><topic>Satellite cells</topic><topic>Schwann cells</topic><topic>Sciatic nerve</topic><topic>Sciatic Nerve - metabolism</topic><topic>Sensory neurons</topic><topic>Sensory Receptor Cells - metabolism</topic><topic>Sensory receptors</topic><topic>Slit protein</topic><topic>Spinal cord</topic><topic>Spinal Cord - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carr, Lauren</creatorcontrib><creatorcontrib>Parkinson, David B</creatorcontrib><creatorcontrib>Dun, Xin-Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carr, Lauren</au><au>Parkinson, David B</au><au>Dun, Xin-Peng</au><au>Gillingwater, Thomas H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-02-24</date><risdate>2017</risdate><volume>12</volume><issue>2</issue><spage>e0172736</spage><epage>e0172736</epage><pages>e0172736-e0172736</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28234971</pmid><doi>10.1371/journal.pone.0172736</doi><tpages>e0172736</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-02, Vol.12 (2), p.e0172736-e0172736 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1871803045 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Axon guidance Axons Axons - metabolism Biology and Life Sciences Blood vessels Blood Vessels - metabolism Brain Cell adhesion & migration Cell migration Cell Movement - genetics Cells (biology) Dentistry Dorsal root ganglia Drosophila Fibroblasts Ganglia, Spinal - metabolism Gene expression Gene Expression Regulation, Developmental Glycoproteins Homeostasis Immunoglobulins Insects Intercellular Signaling Peptides and Proteins - biosynthesis Intercellular Signaling Peptides and Proteins - metabolism Mammals Medicine and Health Sciences Membrane Proteins - biosynthesis Membrane Proteins - metabolism Mice Motor neurons Motor Neurons - metabolism Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Nervous system Neurons Peripheral nerves Peripheral nervous system Peripheral Nervous System - metabolism Phosphorylation Physiological aspects Proteins Receptors Receptors, Immunologic - biosynthesis Receptors, Immunologic - metabolism Research and Analysis Methods Rodents Roundabout Proteins Satellite cells Schwann cells Sciatic nerve Sciatic Nerve - metabolism Sensory neurons Sensory Receptor Cells - metabolism Sensory receptors Slit protein Spinal cord Spinal Cord - metabolism |
| title | Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T11%3A44%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20patterns%20of%20Slit%20and%20Robo%20family%20members%20in%20adult%20mouse%20spinal%20cord%20and%20peripheral%20nervous%20system&rft.jtitle=PloS%20one&rft.au=Carr,%20Lauren&rft.date=2017-02-24&rft.volume=12&rft.issue=2&rft.spage=e0172736&rft.epage=e0172736&rft.pages=e0172736-e0172736&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0172736&rft_dat=%3Cgale_plos_%3EA482540510%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1871803045&rft_id=info:pmid/28234971&rft_galeid=A482540510&rft_doaj_id=oai_doaj_org_article_62a519588e5c4c53aa01c71ef49a7325&rfr_iscdi=true |