The distinct features of microbial 'dysbiosis' of Crohn's disease do not occur to the same extent in their unaffected, genetically-linked kindred
Studying the gut microbiota in unaffected relatives of people with Crohn's disease (CD) may advance our understanding of the role of bacteria in disease aetiology. Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain...
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| creator | Ijaz, Umer Zeeshan Quince, Christopher Hanske, Laura Loman, Nick Calus, Szymon T Bertz, Martin Edwards, Christine A Gaya, Daniel R Hansen, Richard McGrogan, Paraic Russell, Richard K Gerasimidis, Konstantinos |
| description | Studying the gut microbiota in unaffected relatives of people with Crohn's disease (CD) may advance our understanding of the role of bacteria in disease aetiology.
Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain fatty acids (SCFA) were compared in unaffected adult relatives of CD children (CDR, n = 17) and adult healthy controls, unrelated to CD patients (HUC, n = 14). The microbiota characteristics of 19 CD children were used as a benchmark of CD 'dysbiosis'.
The CDR microbiota was less diverse (p = 0.044) than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 (1.8%) operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity (p = 0.207) and SCFA concentration or pattern were similar between CDR and HUC (p>0.05 for all SCFA). None of the KEGG metabolic pathways were different between these two groups. Both of these groups (HUC and CDR) had a higher microbiota α-diversity (CDR, p = 0.026 and HUC, p |
| doi_str_mv | 10.1371/journal.pone.0172605 |
| format | Article |
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Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain fatty acids (SCFA) were compared in unaffected adult relatives of CD children (CDR, n = 17) and adult healthy controls, unrelated to CD patients (HUC, n = 14). The microbiota characteristics of 19 CD children were used as a benchmark of CD 'dysbiosis'.
The CDR microbiota was less diverse (p = 0.044) than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 (1.8%) operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity (p = 0.207) and SCFA concentration or pattern were similar between CDR and HUC (p>0.05 for all SCFA). None of the KEGG metabolic pathways were different between these two groups. Both of these groups (HUC and CDR) had a higher microbiota α-diversity (CDR, p = 0.026 and HUC, p<0.001) with a community structure (β-diversity) distinct from that of children with CD.
While some alterations were observed, a distinct microbial 'dysbiosis', characteristic of CD patients, was not observed in their unaffected, genetically linked kindred.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0172605</identifier><identifier>PMID: 28222161</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Bacteria ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - metabolism ; Biodiversity ; Bioinformatics ; Biology ; Biology and Life Sciences ; Care and treatment ; Child ; Children ; Communities ; Community structure ; Comparative analysis ; Crohn Disease - genetics ; Crohn Disease - microbiology ; Crohn's disease ; Dentistry ; Disease ; Dysbacteriosis ; Dysbiosis - etiology ; Dysbiosis - genetics ; Dysbiosis - microbiology ; Family Health ; Fatty acids ; Fatty Acids, Volatile - analysis ; Feces - chemistry ; Feces - microbiology ; Female ; Gastroenterology ; Gastrointestinal Microbiome ; Gene sequencing ; Genes ; Genetic aspects ; Health aspects ; Hepatology ; Hospitals ; Humans ; Intestinal microflora ; Leukocyte L1 Antigen Complex - analysis ; Life sciences ; Male ; Medicine ; Medicine and Health Sciences ; Metabolic Networks and Pathways ; Metabolic pathways ; Metagenomics ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Nursing ; Nutrition ; Parents ; Patients ; Research and analysis methods ; Ribotyping ; RNA ; rRNA 16S ; Siblings ; Studies</subject><ispartof>PloS one, 2017-02, Vol.12 (2), p.e0172605-e0172605</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Ijaz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Ijaz et al 2017 Ijaz et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-5cb89caa1574b1dbde0cacce9bf944bcc6dc8d25a0e54bc065aa7c444f08c64c3</citedby><cites>FETCH-LOGICAL-c618t-5cb89caa1574b1dbde0cacce9bf944bcc6dc8d25a0e54bc065aa7c444f08c64c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319678/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319678/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28222161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ijaz, Umer Zeeshan</creatorcontrib><creatorcontrib>Quince, Christopher</creatorcontrib><creatorcontrib>Hanske, Laura</creatorcontrib><creatorcontrib>Loman, Nick</creatorcontrib><creatorcontrib>Calus, Szymon T</creatorcontrib><creatorcontrib>Bertz, Martin</creatorcontrib><creatorcontrib>Edwards, Christine A</creatorcontrib><creatorcontrib>Gaya, Daniel R</creatorcontrib><creatorcontrib>Hansen, Richard</creatorcontrib><creatorcontrib>McGrogan, Paraic</creatorcontrib><creatorcontrib>Russell, Richard K</creatorcontrib><creatorcontrib>Gerasimidis, Konstantinos</creatorcontrib><title>The distinct features of microbial 'dysbiosis' of Crohn's disease do not occur to the same extent in their unaffected, genetically-linked kindred</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Studying the gut microbiota in unaffected relatives of people with Crohn's disease (CD) may advance our understanding of the role of bacteria in disease aetiology.
Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain fatty acids (SCFA) were compared in unaffected adult relatives of CD children (CDR, n = 17) and adult healthy controls, unrelated to CD patients (HUC, n = 14). The microbiota characteristics of 19 CD children were used as a benchmark of CD 'dysbiosis'.
The CDR microbiota was less diverse (p = 0.044) than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 (1.8%) operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity (p = 0.207) and SCFA concentration or pattern were similar between CDR and HUC (p>0.05 for all SCFA). None of the KEGG metabolic pathways were different between these two groups. Both of these groups (HUC and CDR) had a higher microbiota α-diversity (CDR, p = 0.026 and HUC, p<0.001) with a community structure (β-diversity) distinct from that of children with CD.
While some alterations were observed, a distinct microbial 'dysbiosis', characteristic of CD patients, was not observed in their unaffected, genetically linked kindred.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Biodiversity</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Children</subject><subject>Communities</subject><subject>Community structure</subject><subject>Comparative analysis</subject><subject>Crohn Disease - genetics</subject><subject>Crohn Disease - microbiology</subject><subject>Crohn's disease</subject><subject>Dentistry</subject><subject>Disease</subject><subject>Dysbacteriosis</subject><subject>Dysbiosis - etiology</subject><subject>Dysbiosis - genetics</subject><subject>Dysbiosis - microbiology</subject><subject>Family Health</subject><subject>Fatty acids</subject><subject>Fatty Acids, Volatile - analysis</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Gastrointestinal Microbiome</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hepatology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Intestinal microflora</subject><subject>Leukocyte L1 Antigen Complex - analysis</subject><subject>Life sciences</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic Networks and Pathways</subject><subject>Metabolic pathways</subject><subject>Metagenomics</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Nursing</subject><subject>Nutrition</subject><subject>Parents</subject><subject>Patients</subject><subject>Research and analysis methods</subject><subject>Ribotyping</subject><subject>RNA</subject><subject>rRNA 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distinct features of microbial 'dysbiosis' of Crohn's disease do not occur to the same extent in their unaffected, genetically-linked kindred</title><author>Ijaz, Umer Zeeshan ; Quince, Christopher ; Hanske, Laura ; Loman, Nick ; Calus, Szymon T ; Bertz, Martin ; Edwards, Christine A ; Gaya, Daniel R ; Hansen, Richard ; McGrogan, Paraic ; Russell, Richard K ; Gerasimidis, Konstantinos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-5cb89caa1574b1dbde0cacce9bf944bcc6dc8d25a0e54bc065aa7c444f08c64c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Biodiversity</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Care and 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One</addtitle><date>2017-02-21</date><risdate>2017</risdate><volume>12</volume><issue>2</issue><spage>e0172605</spage><epage>e0172605</epage><pages>e0172605-e0172605</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Studying the gut microbiota in unaffected relatives of people with Crohn's disease (CD) may advance our understanding of the role of bacteria in disease aetiology.
Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain fatty acids (SCFA) were compared in unaffected adult relatives of CD children (CDR, n = 17) and adult healthy controls, unrelated to CD patients (HUC, n = 14). The microbiota characteristics of 19 CD children were used as a benchmark of CD 'dysbiosis'.
The CDR microbiota was less diverse (p = 0.044) than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 (1.8%) operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity (p = 0.207) and SCFA concentration or pattern were similar between CDR and HUC (p>0.05 for all SCFA). None of the KEGG metabolic pathways were different between these two groups. Both of these groups (HUC and CDR) had a higher microbiota α-diversity (CDR, p = 0.026 and HUC, p<0.001) with a community structure (β-diversity) distinct from that of children with CD.
While some alterations were observed, a distinct microbial 'dysbiosis', characteristic of CD patients, was not observed in their unaffected, genetically linked kindred.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28222161</pmid><doi>10.1371/journal.pone.0172605</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-02, Vol.12 (2), p.e0172605-e0172605 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1870633150 |
| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Freely accessible e-journals; PubMed Central |
| subjects | Adolescent Adult Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Biodiversity Bioinformatics Biology Biology and Life Sciences Care and treatment Child Children Communities Community structure Comparative analysis Crohn Disease - genetics Crohn Disease - microbiology Crohn's disease Dentistry Disease Dysbacteriosis Dysbiosis - etiology Dysbiosis - genetics Dysbiosis - microbiology Family Health Fatty acids Fatty Acids, Volatile - analysis Feces - chemistry Feces - microbiology Female Gastroenterology Gastrointestinal Microbiome Gene sequencing Genes Genetic aspects Health aspects Hepatology Hospitals Humans Intestinal microflora Leukocyte L1 Antigen Complex - analysis Life sciences Male Medicine Medicine and Health Sciences Metabolic Networks and Pathways Metabolic pathways Metagenomics Microbiota Microbiota (Symbiotic organisms) Microorganisms Nursing Nutrition Parents Patients Research and analysis methods Ribotyping RNA rRNA 16S Siblings Studies |
| title | The distinct features of microbial 'dysbiosis' of Crohn's disease do not occur to the same extent in their unaffected, genetically-linked kindred |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T19%3A57%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20distinct%20features%20of%20microbial%20'dysbiosis'%20of%20Crohn's%20disease%20do%20not%20occur%20to%20the%20same%20extent%20in%20their%20unaffected,%20genetically-linked%20kindred&rft.jtitle=PloS%20one&rft.au=Ijaz,%20Umer%20Zeeshan&rft.date=2017-02-21&rft.volume=12&rft.issue=2&rft.spage=e0172605&rft.epage=e0172605&rft.pages=e0172605-e0172605&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0172605&rft_dat=%3Cgale_plos_%3EA481999931%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1870633150&rft_id=info:pmid/28222161&rft_galeid=A481999931&rft_doaj_id=oai_doaj_org_article_ae88952367704d47898c3eb4d19072ac&rfr_iscdi=true |