Muscle Reactive Oxygen Species (ROS) Contribute to Post-Incisional Guarding via the TRPA1 Receptor
Deep tissues and their afferents have unique responses to various stimuli and respond to injury distinctively. However, the types of receptors and endogenous ligands that have a key role in pain after deep tissue incision are unknown. TRPA1 has been shown to mediate pain-related responses in inflamm...
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| description | Deep tissues and their afferents have unique responses to various stimuli and respond to injury distinctively. However, the types of receptors and endogenous ligands that have a key role in pain after deep tissue incision are unknown. TRPA1 has been shown to mediate pain-related responses in inflammation- and nerve injury-induced pain models. We hypothesized that TRPA1 has an important role in pain behaviors after deep tissue incision.
The effect of various doses of intraperitoneal (i.p.) TRPA1 antagonist, HC-030031, on pain behaviors after skin + deep tissue incision of the rat hind paw was measured. In vivo reactive oxygen species (ROS)-imaging and hydrogen peroxide (H2O2) levels after incision were also evaluated. Separate groups of rats were examined for H2O2-evoked pain-related behaviors after injections into the deep tissue or the subcutaneous tissue.
Guarding pain behavior after skin + deep tissue incision was decreased by i.p. HC-030031. However, HC-030031 did not affect mechanical or heat responses after incision. Treatment either before or after incision was effective against incision-induced guarding behavior. ROS increased after skin + deep tissue incision in both the incised muscle and the skin. Tissue H2O2 also increased in both skin and muscle after incision. H2O2 injection produced pain behaviors when injected into muscle but not after subcutaneous injection.
This study demonstrates that TRPA1 antagonist HC-030031 reduced spontaneous guarding pain behavior after skin + deep tissue incision. These data indicate that TRPA1 receptors on nociceptors are active in incised fascia and muscle but this is not evident in incised skin. Even though endogenous TRPA1 agonists like ROS and H2O2 were increased in both incised skin and muscle, those in skin do not contribute to nociceptive behaviors. This study suggests that endogenous TRPA1 ligands and the TRPA1 receptor are important targets for acute pain from deep tissue injury. |
| doi_str_mv | 10.1371/journal.pone.0170410 |
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The effect of various doses of intraperitoneal (i.p.) TRPA1 antagonist, HC-030031, on pain behaviors after skin + deep tissue incision of the rat hind paw was measured. In vivo reactive oxygen species (ROS)-imaging and hydrogen peroxide (H2O2) levels after incision were also evaluated. Separate groups of rats were examined for H2O2-evoked pain-related behaviors after injections into the deep tissue or the subcutaneous tissue.
Guarding pain behavior after skin + deep tissue incision was decreased by i.p. HC-030031. However, HC-030031 did not affect mechanical or heat responses after incision. Treatment either before or after incision was effective against incision-induced guarding behavior. ROS increased after skin + deep tissue incision in both the incised muscle and the skin. Tissue H2O2 also increased in both skin and muscle after incision. H2O2 injection produced pain behaviors when injected into muscle but not after subcutaneous injection.
This study demonstrates that TRPA1 antagonist HC-030031 reduced spontaneous guarding pain behavior after skin + deep tissue incision. These data indicate that TRPA1 receptors on nociceptors are active in incised fascia and muscle but this is not evident in incised skin. Even though endogenous TRPA1 agonists like ROS and H2O2 were increased in both incised skin and muscle, those in skin do not contribute to nociceptive behaviors. This study suggests that endogenous TRPA1 ligands and the TRPA1 receptor are important targets for acute pain from deep tissue injury.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0170410</identifier><identifier>PMID: 28103292</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetanilides - administration & dosage ; Analysis ; Anesthesiology ; Animal models ; Animal tissues ; Animals ; Antagonists (Biochemistry) ; Behavior ; Biology and Life Sciences ; Depth indicators ; Fascia ; Free radicals ; Gene expression ; Growth factors ; Guarding behavior ; Heat treatment ; Hydrogen ; Hydrogen peroxide ; Hydrogen Peroxide - administration & dosage ; Hydrogen Peroxide - metabolism ; Injection ; Injuries ; Laboratory animals ; Ligands ; Male ; Medical research ; Medicine ; Medicine and Health Sciences ; Muscle, Skeletal - innervation ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - surgery ; Muscles ; Neurosciences ; Nociceptors ; Nociceptors - drug effects ; Nociceptors - metabolism ; Oxidative stress ; Oxygen ; Pain ; Pain management ; Pain perception ; Pain Threshold - drug effects ; Pain Threshold - physiology ; Pain, Postoperative - metabolism ; Purines - administration & dosage ; Rats ; Rats, Sprague-Dawley ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Receptors ; Research and Analysis Methods ; Rodents ; Skin ; Skin - innervation ; Skin - metabolism ; Surgery ; Tissues ; TRPA1 Cation Channel ; TRPC Cation Channels - antagonists & inhibitors ; TRPC Cation Channels - metabolism</subject><ispartof>PloS one, 2017-01, Vol.12 (1), p.e0170410-e0170410</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Sugiyama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Sugiyama et al 2017 Sugiyama et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-ce3ed2b36f69704cb8257c9324a9266370ca699af4f764c6f16f79c7d0f704703</citedby><cites>FETCH-LOGICAL-c593t-ce3ed2b36f69704cb8257c9324a9266370ca699af4f764c6f16f79c7d0f704703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245866/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245866/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28103292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugiyama, Daisuke</creatorcontrib><creatorcontrib>Kang, Sinyoung</creatorcontrib><creatorcontrib>Brennan, Timothy J</creatorcontrib><title>Muscle Reactive Oxygen Species (ROS) Contribute to Post-Incisional Guarding via the TRPA1 Receptor</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Deep tissues and their afferents have unique responses to various stimuli and respond to injury distinctively. However, the types of receptors and endogenous ligands that have a key role in pain after deep tissue incision are unknown. TRPA1 has been shown to mediate pain-related responses in inflammation- and nerve injury-induced pain models. We hypothesized that TRPA1 has an important role in pain behaviors after deep tissue incision.
The effect of various doses of intraperitoneal (i.p.) TRPA1 antagonist, HC-030031, on pain behaviors after skin + deep tissue incision of the rat hind paw was measured. In vivo reactive oxygen species (ROS)-imaging and hydrogen peroxide (H2O2) levels after incision were also evaluated. Separate groups of rats were examined for H2O2-evoked pain-related behaviors after injections into the deep tissue or the subcutaneous tissue.
Guarding pain behavior after skin + deep tissue incision was decreased by i.p. HC-030031. However, HC-030031 did not affect mechanical or heat responses after incision. Treatment either before or after incision was effective against incision-induced guarding behavior. ROS increased after skin + deep tissue incision in both the incised muscle and the skin. Tissue H2O2 also increased in both skin and muscle after incision. H2O2 injection produced pain behaviors when injected into muscle but not after subcutaneous injection.
This study demonstrates that TRPA1 antagonist HC-030031 reduced spontaneous guarding pain behavior after skin + deep tissue incision. These data indicate that TRPA1 receptors on nociceptors are active in incised fascia and muscle but this is not evident in incised skin. Even though endogenous TRPA1 agonists like ROS and H2O2 were increased in both incised skin and muscle, those in skin do not contribute to nociceptive behaviors. This study suggests that endogenous TRPA1 ligands and the TRPA1 receptor are important targets for acute pain from deep tissue injury.</description><subject>Acetanilides - administration & dosage</subject><subject>Analysis</subject><subject>Anesthesiology</subject><subject>Animal models</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Antagonists (Biochemistry)</subject><subject>Behavior</subject><subject>Biology and Life Sciences</subject><subject>Depth indicators</subject><subject>Fascia</subject><subject>Free radicals</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Guarding behavior</subject><subject>Heat treatment</subject><subject>Hydrogen</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - administration & dosage</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Injection</subject><subject>Injuries</subject><subject>Laboratory animals</subject><subject>Ligands</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Muscle, Skeletal - innervation</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - surgery</subject><subject>Muscles</subject><subject>Neurosciences</subject><subject>Nociceptors</subject><subject>Nociceptors - drug effects</subject><subject>Nociceptors - metabolism</subject><subject>Oxidative stress</subject><subject>Oxygen</subject><subject>Pain</subject><subject>Pain management</subject><subject>Pain perception</subject><subject>Pain Threshold - drug effects</subject><subject>Pain Threshold - physiology</subject><subject>Pain, Postoperative - metabolism</subject><subject>Purines - administration & dosage</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Receptors</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Skin</subject><subject>Skin - innervation</subject><subject>Skin - metabolism</subject><subject>Surgery</subject><subject>Tissues</subject><subject>TRPA1 Cation Channel</subject><subject>TRPC Cation Channels - antagonists & inhibitors</subject><subject>TRPC Cation Channels - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1v0zAUjRCIjcE_QGCJl_GQ4q_YyQtSVcGoNNSpG8-W49iZqzTubKdi_x5nzaYVTX6wZZ977jnXJ8s-IjhDhKNvGzf4Xnaznev1DCIOKYKvslNUEZwzDMnrZ-eT7F0IGwgLUjL2NjvBJYIEV_g0q38PQXUarLVU0e41WP29b3UPrndaWR3A-Xp1_RUsXB-9rYeoQXTgyoWYL3tlg3VJAbgYpG9s34K9lSDeanCzvpqjRKn0Ljr_PntjZBf0h2k_y_78_HGz-JVfri6Wi_llroqKxFxpohtcE2ZYlcyousQFV8kBlRVmjHCoJKsqaajhjCpmEDO8UryBJsE5JGfZ5wPvrnNBTOMJApUMIshZiRNieUA0Tm7Eztut9PfCSSseLpxvhfTRpnmIBhlpsCwqzgpKmkZKyo0qSyVrY2pCE9f3qdtQb3WjdJqQ7I5Ij196eytatxcFpkX6hURwPhF4dzfoEMXWBqW7TvbaDQ-6UVEiykbdX_6DvuxuQrUyGbC9camvGknFnPISEYLhqHv2AiqtRm-tSlkyNt0fFdBDgfIuBK_Nk0cExZjERzFiTKKYkpjKPj2fz1PRY_TIP_pf2fA</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Sugiyama, Daisuke</creator><creator>Kang, Sinyoung</creator><creator>Brennan, Timothy J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170101</creationdate><title>Muscle Reactive Oxygen Species (ROS) Contribute to Post-Incisional Guarding via the TRPA1 Receptor</title><author>Sugiyama, Daisuke ; Kang, Sinyoung ; Brennan, Timothy J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-ce3ed2b36f69704cb8257c9324a9266370ca699af4f764c6f16f79c7d0f704703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acetanilides - administration & dosage</topic><topic>Analysis</topic><topic>Anesthesiology</topic><topic>Animal models</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Antagonists (Biochemistry)</topic><topic>Behavior</topic><topic>Biology and Life Sciences</topic><topic>Depth indicators</topic><topic>Fascia</topic><topic>Free radicals</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Guarding behavior</topic><topic>Heat treatment</topic><topic>Hydrogen</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - administration & dosage</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Injection</topic><topic>Injuries</topic><topic>Laboratory animals</topic><topic>Ligands</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Muscle, Skeletal - innervation</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - surgery</topic><topic>Muscles</topic><topic>Neurosciences</topic><topic>Nociceptors</topic><topic>Nociceptors - drug effects</topic><topic>Nociceptors - metabolism</topic><topic>Oxidative stress</topic><topic>Oxygen</topic><topic>Pain</topic><topic>Pain management</topic><topic>Pain perception</topic><topic>Pain Threshold - drug effects</topic><topic>Pain Threshold - physiology</topic><topic>Pain, Postoperative - metabolism</topic><topic>Purines - administration & dosage</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Receptors</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Skin</topic><topic>Skin - innervation</topic><topic>Skin - metabolism</topic><topic>Surgery</topic><topic>Tissues</topic><topic>TRPA1 Cation Channel</topic><topic>TRPC Cation Channels - antagonists & inhibitors</topic><topic>TRPC Cation Channels - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugiyama, Daisuke</creatorcontrib><creatorcontrib>Kang, Sinyoung</creatorcontrib><creatorcontrib>Brennan, Timothy J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugiyama, Daisuke</au><au>Kang, Sinyoung</au><au>Brennan, Timothy J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscle Reactive Oxygen Species (ROS) Contribute to Post-Incisional Guarding via the TRPA1 Receptor</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>12</volume><issue>1</issue><spage>e0170410</spage><epage>e0170410</epage><pages>e0170410-e0170410</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Deep tissues and their afferents have unique responses to various stimuli and respond to injury distinctively. However, the types of receptors and endogenous ligands that have a key role in pain after deep tissue incision are unknown. TRPA1 has been shown to mediate pain-related responses in inflammation- and nerve injury-induced pain models. We hypothesized that TRPA1 has an important role in pain behaviors after deep tissue incision.
The effect of various doses of intraperitoneal (i.p.) TRPA1 antagonist, HC-030031, on pain behaviors after skin + deep tissue incision of the rat hind paw was measured. In vivo reactive oxygen species (ROS)-imaging and hydrogen peroxide (H2O2) levels after incision were also evaluated. Separate groups of rats were examined for H2O2-evoked pain-related behaviors after injections into the deep tissue or the subcutaneous tissue.
Guarding pain behavior after skin + deep tissue incision was decreased by i.p. HC-030031. However, HC-030031 did not affect mechanical or heat responses after incision. Treatment either before or after incision was effective against incision-induced guarding behavior. ROS increased after skin + deep tissue incision in both the incised muscle and the skin. Tissue H2O2 also increased in both skin and muscle after incision. H2O2 injection produced pain behaviors when injected into muscle but not after subcutaneous injection.
This study demonstrates that TRPA1 antagonist HC-030031 reduced spontaneous guarding pain behavior after skin + deep tissue incision. These data indicate that TRPA1 receptors on nociceptors are active in incised fascia and muscle but this is not evident in incised skin. Even though endogenous TRPA1 agonists like ROS and H2O2 were increased in both incised skin and muscle, those in skin do not contribute to nociceptive behaviors. This study suggests that endogenous TRPA1 ligands and the TRPA1 receptor are important targets for acute pain from deep tissue injury.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28103292</pmid><doi>10.1371/journal.pone.0170410</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| subjects | Acetanilides - administration & dosage Analysis Anesthesiology Animal models Animal tissues Animals Antagonists (Biochemistry) Behavior Biology and Life Sciences Depth indicators Fascia Free radicals Gene expression Growth factors Guarding behavior Heat treatment Hydrogen Hydrogen peroxide Hydrogen Peroxide - administration & dosage Hydrogen Peroxide - metabolism Injection Injuries Laboratory animals Ligands Male Medical research Medicine Medicine and Health Sciences Muscle, Skeletal - innervation Muscle, Skeletal - metabolism Muscle, Skeletal - surgery Muscles Neurosciences Nociceptors Nociceptors - drug effects Nociceptors - metabolism Oxidative stress Oxygen Pain Pain management Pain perception Pain Threshold - drug effects Pain Threshold - physiology Pain, Postoperative - metabolism Purines - administration & dosage Rats Rats, Sprague-Dawley Reactive oxygen species Reactive Oxygen Species - metabolism Receptors Research and Analysis Methods Rodents Skin Skin - innervation Skin - metabolism Surgery Tissues TRPA1 Cation Channel TRPC Cation Channels - antagonists & inhibitors TRPC Cation Channels - metabolism |
| title | Muscle Reactive Oxygen Species (ROS) Contribute to Post-Incisional Guarding via the TRPA1 Receptor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T10%3A29%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Muscle%20Reactive%20Oxygen%20Species%20(ROS)%20Contribute%20to%20Post-Incisional%20Guarding%20via%20the%20TRPA1%20Receptor&rft.jtitle=PloS%20one&rft.au=Sugiyama,%20Daisuke&rft.date=2017-01-01&rft.volume=12&rft.issue=1&rft.spage=e0170410&rft.epage=e0170410&rft.pages=e0170410-e0170410&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0170410&rft_dat=%3Cgale_plos_%3EA478133204%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1860107682&rft_id=info:pmid/28103292&rft_galeid=A478133204&rft_doaj_id=oai_doaj_org_article_d1faf2a5976543ddaa47fc88cabffb34&rfr_iscdi=true |