A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation
Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynt...
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| creator | Embaby, Amira M Melika, Ramy R Hussein, Ahmed El-Kamel, Amal H Marey, Heba S |
| description | Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on citrus pectin-based medium as a core POS production medium. POS' levels accumulated in the fungal fermentation broth were optimized through a three step sequential statistical mathematical methodology; Plackett-Burman design (PBD), Box-Behnken design (BBD) and canonical analysis. Three key determinants namely citrus pectin, peptone and NaH2PO4 were pointed out by PBD to impose significant consequences (P |
| doi_str_mv | 10.1371/journal.pone.0167981 |
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Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Embaby, Amira M ; Melika, Ramy R ; Hussein, Ahmed ; El-Kamel, Amal H ; Marey, Heba S</creator><contributor>Papp, Tamás</contributor><creatorcontrib>Embaby, Amira M ; Melika, Ramy R ; Hussein, Ahmed ; El-Kamel, Amal H ; Marey, Heba S ; Papp, Tamás</creatorcontrib><description>Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on citrus pectin-based medium as a core POS production medium. POS' levels accumulated in the fungal fermentation broth were optimized through a three step sequential statistical mathematical methodology; Plackett-Burman design (PBD), Box-Behnken design (BBD) and canonical analysis. Three key determinants namely citrus pectin, peptone and NaH2PO4 were pointed out by PBD to impose significant consequences (P<0.05) on the process outcome (POS' levels). Optimal levels of these key determinants along with maximal of POS' levels were set by BBD and canonical analysis to be 2.28% (w/v) citrus pectin, 0.026% (w/v) peptone and 0.28% (w/v) NaH2PO4 to achieve a net amount of 1.3 g POS /2.28 g citrus pectin. Through this approach, a yield of 57% (w/w) POS of the total citrus pectin was obtained after 24 h of fungal growth on optimized citrus pectin-based medium. A fold enhancement of 13 times in POS' levels released in the fermentation fungal broth was realized by the end of the optimization strategy. This novel robust approach is considered a new insight towards POS biosynthesis via efficient, rapid and non-cumbersome procedure. To the best of authors' knowledge, the present work is the first article underlining detailed POS production from the fermentation broth of a fungus growing on citrus pectin-based medium.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167981</identifier><identifier>PMID: 27992459</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Aspergillus ; Aspergillus - growth & development ; Aspergillus - metabolism ; Bacillus licheniformis ; Biology and Life Sciences ; Biosynthesis ; Biotechnology ; Carbohydrate Metabolism ; Citrus ; Citrus fruits ; Enzymes ; Feed industry ; Fermentation ; Food Industry ; Fungi ; Graduate studies ; Hydrogen-Ion Concentration ; Membrane reactors ; Methods ; Oligosaccharides ; Oligosaccharides - biosynthesis ; Optimization ; Pectin ; Pectins - chemistry ; Peptones ; Peptones - chemistry ; Phosphates - chemistry ; Physical Sciences ; Physiological aspects ; Robustness (mathematics) ; Studies</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0167981-e0167981</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Embaby et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Embaby et al 2016 Embaby et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-98e9dbc7b80608db3275e49aab35c77a075868a853335de05a4736e668dbee9a3</citedby><cites>FETCH-LOGICAL-c725t-98e9dbc7b80608db3275e49aab35c77a075868a853335de05a4736e668dbee9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167267/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167267/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27992459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Papp, Tamás</contributor><creatorcontrib>Embaby, Amira M</creatorcontrib><creatorcontrib>Melika, Ramy R</creatorcontrib><creatorcontrib>Hussein, Ahmed</creatorcontrib><creatorcontrib>El-Kamel, Amal H</creatorcontrib><creatorcontrib>Marey, Heba S</creatorcontrib><title>A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. 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Optimal levels of these key determinants along with maximal of POS' levels were set by BBD and canonical analysis to be 2.28% (w/v) citrus pectin, 0.026% (w/v) peptone and 0.28% (w/v) NaH2PO4 to achieve a net amount of 1.3 g POS /2.28 g citrus pectin. Through this approach, a yield of 57% (w/w) POS of the total citrus pectin was obtained after 24 h of fungal growth on optimized citrus pectin-based medium. A fold enhancement of 13 times in POS' levels released in the fermentation fungal broth was realized by the end of the optimization strategy. This novel robust approach is considered a new insight towards POS biosynthesis via efficient, rapid and non-cumbersome procedure. To the best of authors' knowledge, the present work is the first article underlining detailed POS production from the fermentation broth of a fungus growing on citrus pectin-based medium.</description><subject>Analysis</subject><subject>Aspergillus</subject><subject>Aspergillus - growth & development</subject><subject>Aspergillus - metabolism</subject><subject>Bacillus licheniformis</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carbohydrate Metabolism</subject><subject>Citrus</subject><subject>Citrus fruits</subject><subject>Enzymes</subject><subject>Feed industry</subject><subject>Fermentation</subject><subject>Food Industry</subject><subject>Fungi</subject><subject>Graduate studies</subject><subject>Hydrogen-Ion Concentration</subject><subject>Membrane reactors</subject><subject>Methods</subject><subject>Oligosaccharides</subject><subject>Oligosaccharides - biosynthesis</subject><subject>Optimization</subject><subject>Pectin</subject><subject>Pectins - chemistry</subject><subject>Peptones</subject><subject>Peptones - chemistry</subject><subject>Phosphates - chemistry</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Robustness (mathematics)</subject><subject>Studies</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk2Fv1CAYxxujcfP0GxglMTH64k4oLZQ3S85zm5dMZ7xp4itCKW1ZaKnQzu27-eGkW7fcmb1YmlACv_-fh4fniaKXCC4QpujDuR1cK8yis61aQEQoy9CjaB8xHM9JDPHjrfle9Mz7cwhTnBHyNNqLKWNxkrL96O8SfLUXyoSxna-GJlfO20aBZdc5K2QNzuwf4QoPPmrrr9q-Vl57YEvwTcley_mp0ZX1QspaOF0oD_Kra6tlaURvL3WlWi3B0nfKVdqYwQPfLcBmFNsWHBlxocGmd0K34PD4FwKfNl8AgiiNIehrZ4eqBivdu6C7PjBIlGtU24tR_zx6Ugrj1YvpP4t-HB2erT7PT06P16vlyVzSOO3nLFOsyCXNM0hgVuQ4pqlKmBA5TiWlAtI0I5nIUoxxWiiYioRioggJrFJM4Fn0-sa3M9bzKfGeoyxlScIgJoFY3xCFFee8c7oR7opbofn1gnUVFy7kyyieSYZQnhNKYJnQsmQQlRkjBYExCjGx4HUwnTbkjSpkuK0TZsd0d6fVNa_sBU9DEcSEBoN3k4Gzvwfle95oL5UxolV2mOKmKWboISiKGYxDDc2iN_-h9ydioioR7qrb0oYQ5WjKlwkdIRSqcBYt7qHCV6hGy1DQpQ7rO4L3O4LA9Oqyr8TgPV9vvj-cPf25y77dYmslTF97a4axuvwumNyA0lnvnSrv3gNBPvbjbTb42I986scge7X9lnei2wbE_wAt3zBF</recordid><startdate>20161219</startdate><enddate>20161219</enddate><creator>Embaby, Amira M</creator><creator>Melika, Ramy R</creator><creator>Hussein, Ahmed</creator><creator>El-Kamel, Amal H</creator><creator>Marey, Heba S</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20161219</creationdate><title>A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. 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Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-19</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0167981</spage><epage>e0167981</epage><pages>e0167981-e0167981</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on citrus pectin-based medium as a core POS production medium. POS' levels accumulated in the fungal fermentation broth were optimized through a three step sequential statistical mathematical methodology; Plackett-Burman design (PBD), Box-Behnken design (BBD) and canonical analysis. Three key determinants namely citrus pectin, peptone and NaH2PO4 were pointed out by PBD to impose significant consequences (P<0.05) on the process outcome (POS' levels). Optimal levels of these key determinants along with maximal of POS' levels were set by BBD and canonical analysis to be 2.28% (w/v) citrus pectin, 0.026% (w/v) peptone and 0.28% (w/v) NaH2PO4 to achieve a net amount of 1.3 g POS /2.28 g citrus pectin. Through this approach, a yield of 57% (w/w) POS of the total citrus pectin was obtained after 24 h of fungal growth on optimized citrus pectin-based medium. A fold enhancement of 13 times in POS' levels released in the fermentation fungal broth was realized by the end of the optimization strategy. This novel robust approach is considered a new insight towards POS biosynthesis via efficient, rapid and non-cumbersome procedure. To the best of authors' knowledge, the present work is the first article underlining detailed POS production from the fermentation broth of a fungus growing on citrus pectin-based medium.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27992459</pmid><doi>10.1371/journal.pone.0167981</doi><tpages>e0167981</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Aspergillus Aspergillus - growth & development Aspergillus - metabolism Bacillus licheniformis Biology and Life Sciences Biosynthesis Biotechnology Carbohydrate Metabolism Citrus Citrus fruits Enzymes Feed industry Fermentation Food Industry Fungi Graduate studies Hydrogen-Ion Concentration Membrane reactors Methods Oligosaccharides Oligosaccharides - biosynthesis Optimization Pectin Pectins - chemistry Peptones Peptones - chemistry Phosphates - chemistry Physical Sciences Physiological aspects Robustness (mathematics) Studies |
| title | A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation |
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