Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria
Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection report...
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| creator | Bukbuk, David N Dowall, Stuart D Lewandowski, Kuiama Bosworth, Andrew Baba, Saka S Varghese, Anitha Watson, Robert J Bell, Andrew Atkinson, Barry Hewson, Roger |
| description | Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria.
Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies. The positivity rates for IgG and IgM were 10.6% and 3.5%, respectively. Additionally, sera from undiagnosed febrile patients (n = 380) were assessed by RT-PCR assay for the presence of CCHFV RNA. One positive sample was characterised by further by next generation sequencing (NGS) resulting in complete S, M and L segment sequences.
This article provides evidence for the continued exposure of the human population of Nigeria to CCHFV. The genomic analysis provides the first published evidence of a human case of CCHFV in Nigeria and its phylogenetic context. |
| doi_str_mv | 10.1371/journal.pntd.0005126 |
| format | Article |
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Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies. The positivity rates for IgG and IgM were 10.6% and 3.5%, respectively. Additionally, sera from undiagnosed febrile patients (n = 380) were assessed by RT-PCR assay for the presence of CCHFV RNA. One positive sample was characterised by further by next generation sequencing (NGS) resulting in complete S, M and L segment sequences.
This article provides evidence for the continued exposure of the human population of Nigeria to CCHFV. The genomic analysis provides the first published evidence of a human case of CCHFV in Nigeria and its phylogenetic context.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0005126</identifier><identifier>PMID: 27926935</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Antibodies ; Antibodies, Viral - blood ; Arachnids ; Biology and Life Sciences ; Cattle ; Colleges & universities ; Diagnosis ; Disease ; Fever ; Funding ; Health aspects ; Hemorrhagic Fever Virus, Crimean-Congo - classification ; Hemorrhagic Fever Virus, Crimean-Congo - genetics ; Hemorrhagic Fever Virus, Crimean-Congo - immunology ; Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification ; Hemorrhagic Fever, Crimean - blood ; Hemorrhagic Fever, Crimean - epidemiology ; Hemorrhagic Fever, Crimean - virology ; Hemorrhagic fevers ; Human populations ; Humans ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Immunoglobulins ; Laboratories ; Local government ; Medicine and health sciences ; Nigeria - epidemiology ; Nosocomial infections ; People and Places ; Phylogenetics ; Phylogeny ; Physiological aspects ; Polymerase chain reaction ; Population ; Public health ; Research and Analysis Methods ; Risk factors ; Seroepidemiologic Studies ; Tropical diseases ; Viruses ; Zoonoses</subject><ispartof>PLoS neglected tropical diseases, 2016-12, Vol.10 (12), p.e0005126-e0005126</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Bukbuk DN, Dowall SD, Lewandowski K, Bosworth A, Baba SS, Varghese A, et al. (2016) Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria. PLoS Negl Trop Dis 10(12): e0005126. doi:10.1371/journal.pntd.0005126</rights><rights>2016 Bukbuk et al 2016 Bukbuk et al</rights><rights>2016 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Bukbuk DN, Dowall SD, Lewandowski K, Bosworth A, Baba SS, Varghese A, et al. (2016) Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria. PLoS Negl Trop Dis 10(12): e0005126. doi:10.1371/journal.pntd.0005126</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c690t-2be9627a1c3d3fe704fe8e71723f29f50d5ffd65e7ae82fa571a0aacb0a770673</citedby><cites>FETCH-LOGICAL-c690t-2be9627a1c3d3fe704fe8e71723f29f50d5ffd65e7ae82fa571a0aacb0a770673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142770/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142770/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27926935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Papa, Anna</contributor><creatorcontrib>Bukbuk, David N</creatorcontrib><creatorcontrib>Dowall, Stuart D</creatorcontrib><creatorcontrib>Lewandowski, Kuiama</creatorcontrib><creatorcontrib>Bosworth, Andrew</creatorcontrib><creatorcontrib>Baba, Saka S</creatorcontrib><creatorcontrib>Varghese, Anitha</creatorcontrib><creatorcontrib>Watson, Robert J</creatorcontrib><creatorcontrib>Bell, Andrew</creatorcontrib><creatorcontrib>Atkinson, Barry</creatorcontrib><creatorcontrib>Hewson, Roger</creatorcontrib><title>Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria.
Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies. The positivity rates for IgG and IgM were 10.6% and 3.5%, respectively. Additionally, sera from undiagnosed febrile patients (n = 380) were assessed by RT-PCR assay for the presence of CCHFV RNA. One positive sample was characterised by further by next generation sequencing (NGS) resulting in complete S, M and L segment sequences.
This article provides evidence for the continued exposure of the human population of Nigeria to CCHFV. The genomic analysis provides the first published evidence of a human case of CCHFV in Nigeria and its phylogenetic context.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Arachnids</subject><subject>Biology and Life Sciences</subject><subject>Cattle</subject><subject>Colleges & universities</subject><subject>Diagnosis</subject><subject>Disease</subject><subject>Fever</subject><subject>Funding</subject><subject>Health aspects</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - classification</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - genetics</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - immunology</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification</subject><subject>Hemorrhagic Fever, Crimean - blood</subject><subject>Hemorrhagic Fever, Crimean - epidemiology</subject><subject>Hemorrhagic Fever, Crimean - virology</subject><subject>Hemorrhagic fevers</subject><subject>Human populations</subject><subject>Humans</subject><subject>Immunoglobulin G - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bukbuk, David N</au><au>Dowall, Stuart D</au><au>Lewandowski, Kuiama</au><au>Bosworth, Andrew</au><au>Baba, Saka S</au><au>Varghese, Anitha</au><au>Watson, Robert J</au><au>Bell, Andrew</au><au>Atkinson, Barry</au><au>Hewson, Roger</au><au>Papa, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2016-12-07</date><risdate>2016</risdate><volume>10</volume><issue>12</issue><spage>e0005126</spage><epage>e0005126</epage><pages>e0005126-e0005126</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria.
Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies. The positivity rates for IgG and IgM were 10.6% and 3.5%, respectively. Additionally, sera from undiagnosed febrile patients (n = 380) were assessed by RT-PCR assay for the presence of CCHFV RNA. One positive sample was characterised by further by next generation sequencing (NGS) resulting in complete S, M and L segment sequences.
This article provides evidence for the continued exposure of the human population of Nigeria to CCHFV. The genomic analysis provides the first published evidence of a human case of CCHFV in Nigeria and its phylogenetic context.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27926935</pmid><doi>10.1371/journal.pntd.0005126</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PLoS neglected tropical diseases, 2016-12, Vol.10 (12), p.e0005126-e0005126 |
| issn | 1935-2735 1935-2727 1935-2735 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis Animals Antibodies Antibodies, Viral - blood Arachnids Biology and Life Sciences Cattle Colleges & universities Diagnosis Disease Fever Funding Health aspects Hemorrhagic Fever Virus, Crimean-Congo - classification Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - immunology Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification Hemorrhagic Fever, Crimean - blood Hemorrhagic Fever, Crimean - epidemiology Hemorrhagic Fever, Crimean - virology Hemorrhagic fevers Human populations Humans Immunoglobulin G - blood Immunoglobulin M - blood Immunoglobulins Laboratories Local government Medicine and health sciences Nigeria - epidemiology Nosocomial infections People and Places Phylogenetics Phylogeny Physiological aspects Polymerase chain reaction Population Public health Research and Analysis Methods Risk factors Seroepidemiologic Studies Tropical diseases Viruses Zoonoses |
| title | Serological and Virological Evidence of Crimean-Congo Haemorrhagic Fever Virus Circulation in the Human Population of Borno State, Northeastern Nigeria |
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