Characterization and Functional Analysis of Five MADS-Box B Class Genes Related to Floral Organ Identification in Tagetes erecta
According to the floral organ development ABC model, B class genes specify petal and stamen identification. In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. S...
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| description | According to the floral organ development ABC model, B class genes specify petal and stamen identification. In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. Sequence comparisons and phylogenetic analyses indicated that there were one PI-like gene-TePI, two euAP3-like genes-TeAP3-1 and TeAP3-2, and two TM6-like genes-TeTM6-1 and TeTM6-2 in T. erecta. Strong expression levels of these genes were detected in stamens of the disk florets, but little or no expression was detected in bracts, receptacles or vegetative organs. Yeast hybrid experiments of the B class proteins showed that TePI protein could form a homodimer and heterodimers with all the other four B class proteins TeAP3-1, TeAP3-2, TeTM6-1 and TeTM6-2. No homodimer or interaction was observed between the euAP3 and TM6 clade members. Over-expression of five B class genes of T. erecta in Nicotiana rotundifolia showed that only the transgenic plants of 35S::TePI showed altered floral morphology compared with the non-transgenic line. This study could contribute to the understanding of the function of B class genes in flower development of T. erecta, and provide a theoretical basis for further research to change floral organ structures and create new materials for plant breeding. |
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In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. Sequence comparisons and phylogenetic analyses indicated that there were one PI-like gene-TePI, two euAP3-like genes-TeAP3-1 and TeAP3-2, and two TM6-like genes-TeTM6-1 and TeTM6-2 in T. erecta. Strong expression levels of these genes were detected in stamens of the disk florets, but little or no expression was detected in bracts, receptacles or vegetative organs. Yeast hybrid experiments of the B class proteins showed that TePI protein could form a homodimer and heterodimers with all the other four B class proteins TeAP3-1, TeAP3-2, TeTM6-1 and TeTM6-2. No homodimer or interaction was observed between the euAP3 and TM6 clade members. Over-expression of five B class genes of T. erecta in Nicotiana rotundifolia showed that only the transgenic plants of 35S::TePI showed altered floral morphology compared with the non-transgenic line. This study could contribute to the understanding of the function of B class genes in flower development of T. erecta, and provide a theoretical basis for further research to change floral organ structures and create new materials for plant breeding.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0169777</identifier><identifier>PMID: 28081202</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino Acid Sequence ; Asteraceae ; Biology and Life Sciences ; Calendula ; Construction ; Dimerization ; Education ; Flowers & plants ; Flowers - genetics ; Flowers - metabolism ; Functional analysis ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Genomics ; Horticulture ; Laboratories ; MADS Domain Proteins - classification ; MADS Domain Proteins - genetics ; MADS Domain Proteins - metabolism ; Marigolds ; Molecular Sequence Data ; Nicotiana ; Nicotiana - genetics ; Nicotiana - metabolism ; Organs ; Overexpression ; Phenotype ; Phylogeny ; Physiological aspects ; Plant biology ; Plant breeding ; Plant Proteins - classification ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants (botany) ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Protein Isoforms - chemistry ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Proteins ; Receptacles ; Research and Analysis Methods ; RNA, Plant - genetics ; RNA, Plant - isolation & purification ; RNA, Plant - metabolism ; Sequence Alignment ; Stamens ; Tagetes - classification ; Tagetes - genetics ; Tagetes - growth & development ; Tagetes - metabolism ; Tagetes erecta ; Transgenic plants ; Two-Hybrid System Techniques ; Yeast</subject><ispartof>PloS one, 2017-01, Vol.12 (1), p.e0169777-e0169777</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Ai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Ai et al 2017 Ai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-f32fc23a835b697fee176918dffd87aa876e22d80e494de8638f7a062678149d3</citedby><cites>FETCH-LOGICAL-c791t-f32fc23a835b697fee176918dffd87aa876e22d80e494de8638f7a062678149d3</cites><orcidid>0000-0002-3502-5166</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5231280/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5231280/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28081202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aceto, Serena</contributor><creatorcontrib>Ai, Ye</creatorcontrib><creatorcontrib>Zhang, Chunling</creatorcontrib><creatorcontrib>Sun, Yalin</creatorcontrib><creatorcontrib>Wang, Weining</creatorcontrib><creatorcontrib>He, Yanhong</creatorcontrib><creatorcontrib>Bao, Manzhu</creatorcontrib><title>Characterization and Functional Analysis of Five MADS-Box B Class Genes Related to Floral Organ Identification in Tagetes erecta</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>According to the floral organ development ABC model, B class genes specify petal and stamen identification. In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. Sequence comparisons and phylogenetic analyses indicated that there were one PI-like gene-TePI, two euAP3-like genes-TeAP3-1 and TeAP3-2, and two TM6-like genes-TeTM6-1 and TeTM6-2 in T. erecta. Strong expression levels of these genes were detected in stamens of the disk florets, but little or no expression was detected in bracts, receptacles or vegetative organs. Yeast hybrid experiments of the B class proteins showed that TePI protein could form a homodimer and heterodimers with all the other four B class proteins TeAP3-1, TeAP3-2, TeTM6-1 and TeTM6-2. No homodimer or interaction was observed between the euAP3 and TM6 clade members. Over-expression of five B class genes of T. erecta in Nicotiana rotundifolia showed that only the transgenic plants of 35S::TePI showed altered floral morphology compared with the non-transgenic line. This study could contribute to the understanding of the function of B class genes in flower development of T. erecta, and provide a theoretical basis for further research to change floral organ structures and create new materials for plant breeding.</description><subject>Amino Acid Sequence</subject><subject>Asteraceae</subject><subject>Biology and Life Sciences</subject><subject>Calendula</subject><subject>Construction</subject><subject>Dimerization</subject><subject>Education</subject><subject>Flowers & plants</subject><subject>Flowers - genetics</subject><subject>Flowers - metabolism</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genomics</subject><subject>Horticulture</subject><subject>Laboratories</subject><subject>MADS Domain Proteins - classification</subject><subject>MADS Domain Proteins - genetics</subject><subject>MADS Domain Proteins - metabolism</subject><subject>Marigolds</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Organs</subject><subject>Overexpression</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Plant biology</subject><subject>Plant breeding</subject><subject>Plant Proteins - classification</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants (botany)</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Proteins</subject><subject>Receptacles</subject><subject>Research and Analysis Methods</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - isolation & purification</subject><subject>RNA, Plant - metabolism</subject><subject>Sequence Alignment</subject><subject>Stamens</subject><subject>Tagetes - classification</subject><subject>Tagetes - genetics</subject><subject>Tagetes - growth & development</subject><subject>Tagetes - metabolism</subject><subject>Tagetes erecta</subject><subject>Transgenic plants</subject><subject>Two-Hybrid System Techniques</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1Fv0zAUhSMEYmPwDxBYQkLw0BLbie28IHWFjkpDk7bBq3XnXLee0rjYybTxxE_Hod20oj1MkRLH-c7J9bFvlr2m-ZhyST9d-j600IzXvsVxTkUlpXyS7dOKs5FgOX96b7yXvYjxMs9LroR4nu0xlSvKcraf_ZkuIYDpMLjf0DnfEmhrMutbM7xAQybpdhNdJN6SmbtC8n3y5Wx06K_JIZk2ECM5whYjOcUGOqxJ58ms8SEpT8ICWjKvse2cdWbj7lpyDgvskgIDmg5eZs8sNBFfbZ8H2Y_Z1_Ppt9HxydF8OjkeGVnRbmQ5s4ZxULy8SEu1iFSKiqra2lpJACUFMlarHIuqqFEJrqyEXDAhFS2qmh9kbze-68ZHvQ0vaqrKlEXFS56I-YaoPVzqdXArCDfag9P_JnxYaAidMw3qHKUqbCUEp6JIVQFIOZQgreKqMpi8Pm__1l-ssDYpgxTJjunul9Yt9cJf6ZJxmnYnGXzYGgT_q8fY6ZWLBpsGWvT9ULdQPFdlSR-D0kJSyoqEvvsPfTiILbWAtFbXWp9KNIOpnhRSsoorPlDjB6h01bhyJh1K69L8juDjjiAxHV53C-hj1POz08ezJz932ff32CVC0y2jb_rhvMVdsNiAJvgYA9q7_aC5HnrqNg099JTe9lSSvbm_l3ei2ybifwECrxpm</recordid><startdate>20170112</startdate><enddate>20170112</enddate><creator>Ai, Ye</creator><creator>Zhang, Chunling</creator><creator>Sun, Yalin</creator><creator>Wang, Weining</creator><creator>He, Yanhong</creator><creator>Bao, Manzhu</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3502-5166</orcidid></search><sort><creationdate>20170112</creationdate><title>Characterization and Functional Analysis of Five MADS-Box B Class Genes Related to Floral Organ Identification in Tagetes erecta</title><author>Ai, Ye ; Zhang, Chunling ; Sun, Yalin ; Wang, Weining ; He, Yanhong ; Bao, Manzhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-f32fc23a835b697fee176918dffd87aa876e22d80e494de8638f7a062678149d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amino Acid Sequence</topic><topic>Asteraceae</topic><topic>Biology and Life Sciences</topic><topic>Calendula</topic><topic>Construction</topic><topic>Dimerization</topic><topic>Education</topic><topic>Flowers & plants</topic><topic>Flowers - 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In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. Sequence comparisons and phylogenetic analyses indicated that there were one PI-like gene-TePI, two euAP3-like genes-TeAP3-1 and TeAP3-2, and two TM6-like genes-TeTM6-1 and TeTM6-2 in T. erecta. Strong expression levels of these genes were detected in stamens of the disk florets, but little or no expression was detected in bracts, receptacles or vegetative organs. Yeast hybrid experiments of the B class proteins showed that TePI protein could form a homodimer and heterodimers with all the other four B class proteins TeAP3-1, TeAP3-2, TeTM6-1 and TeTM6-2. No homodimer or interaction was observed between the euAP3 and TM6 clade members. Over-expression of five B class genes of T. erecta in Nicotiana rotundifolia showed that only the transgenic plants of 35S::TePI showed altered floral morphology compared with the non-transgenic line. This study could contribute to the understanding of the function of B class genes in flower development of T. erecta, and provide a theoretical basis for further research to change floral organ structures and create new materials for plant breeding.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28081202</pmid><doi>10.1371/journal.pone.0169777</doi><tpages>e0169777</tpages><orcidid>https://orcid.org/0000-0002-3502-5166</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Asteraceae Biology and Life Sciences Calendula Construction Dimerization Education Flowers & plants Flowers - genetics Flowers - metabolism Functional analysis Gene expression Gene Expression Regulation, Plant Genes Genomics Horticulture Laboratories MADS Domain Proteins - classification MADS Domain Proteins - genetics MADS Domain Proteins - metabolism Marigolds Molecular Sequence Data Nicotiana Nicotiana - genetics Nicotiana - metabolism Organs Overexpression Phenotype Phylogeny Physiological aspects Plant biology Plant breeding Plant Proteins - classification Plant Proteins - genetics Plant Proteins - metabolism Plants (botany) Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Protein Isoforms - chemistry Protein Isoforms - genetics Protein Isoforms - metabolism Proteins Receptacles Research and Analysis Methods RNA, Plant - genetics RNA, Plant - isolation & purification RNA, Plant - metabolism Sequence Alignment Stamens Tagetes - classification Tagetes - genetics Tagetes - growth & development Tagetes - metabolism Tagetes erecta Transgenic plants Two-Hybrid System Techniques Yeast |
| title | Characterization and Functional Analysis of Five MADS-Box B Class Genes Related to Floral Organ Identification in Tagetes erecta |
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