Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients
Numerous studies have shown Kinesin family member 20A (KIF20A) may play a critical role in the development and progression of cancer. However, the clinical value of KIF20A in nasopharyngeal carcinoma (NPC) is unknown. Here, we investigated the expression pattern of KIF20A in NPC and its correlation...
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| creator | Liu, Sai-Lan Lin, Huan-Xin Qiu, Fang Zhang, Wei-Jing Niu, Chun-Hao Wen, Wen Sun, Xiao-Qing Ye, Li-Ping Wu, Xian-Qiu Lin, Chu-Yong Song, Li-Bing Guo, Ling |
| description | Numerous studies have shown Kinesin family member 20A (KIF20A) may play a critical role in the development and progression of cancer. However, the clinical value of KIF20A in nasopharyngeal carcinoma (NPC) is unknown. Here, we investigated the expression pattern of KIF20A in NPC and its correlation with clinicopathological features of patients.
Real-time PCR and Western blotting were used to quantify KIF20A expression in NPC cell lines and clinical specimens compared with normal controls. KIF20A protein expression was also examined in archived paraffin embedded tumor samples from 105 patients with pathologically confirmed NPC by immunohistochemistry (IHC). Statistical analyses were applied to assess the associations between KIF20A expression and the clinicopathological features and survival outcomes. Effects on migration and invasion were assessed by wound healing and transwell invasion assays after KIF20A silencing.
KIF20A was significantly overexpressed at both the mRNA and protein levels in NPC cell lines and human tumor tissues. 45/105 (42.9%) of NPC specimens expressed high levels of KIF20A among the KIF20A detectable cases. Statistical analysis revealed that high KIF20A expression was significantly associated with gender (P = 0.046), clinical stage (P |
| doi_str_mv | 10.1371/journal.pone.0169280 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1858089279</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477293889</galeid><doaj_id>oai_doaj_org_article_eabb4e9963d64b81ae3d43365905c836</doaj_id><sourcerecordid>A477293889</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-a87f562cfda55ac158728bdadd127edb42467806d7ffcebd27e7dcc37b50248f3</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBYQkJw0eKPJHa4QKoKYxWDTePj1nLsk9ZVYhc7Gdtv4k_ibu20ol1MuUh08ryvzzk-J8ueEzwmjJN3Sz8Ep9rxyjsYY1JWVOAH2T6pGB2VFLOHt773sicxLjEumCjLx9leQgUhTOxnf0_OIcDFKkCM1jvkG_TFOojWoUPV2fYSfYWuhoAonqCpDwFa1UNEf2y_QB9tBBUBnQY_3xooZ9Cp9-Eq6Hy0ESWvo6FTDn1T0a8WKly6OagWTZXTEN6jCTqDPvi4At3bc0CTVNblWpiSIbhAp6q34Pr4NHvUqDbCs837IPt5-OnH9Gh0fPJ5Np0cjzSnRT9SgjdFSXVjVFEoTQrBqaiNMoZQDqbOaV5ygUvDm0ZDbVKQG60ZrwtMc9Gwg-zlte-q9VFu-hwlEUVqW0V5lYjZNWG8WspVsF0qSnpl5VXAh7lUobe6BQmqrnOoqpKZMq8FUcBMzlhZVLjQgpXJ68PmtKHuwOhUaVDtjunuH2cXcu7PZUEZ5pwkgzcbg-B_DxB72dmooW2VAz-s8y4FwxUW_D4oyRNNRUJf_Yfe3YgNNVepVusan1LUa1M5yTmnFRNiTY3voNJjoLM6zW9jU3xH8HZHkJgeLvq5GmKUs-9n92dPfu2yr2-xizSE_SL6dujT5MZdML8GdZrLGKC5uQ-C5Xr9tt2Q6_WTm_VLshe37_JGtN039g9gMSyL</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1858089279</pqid></control><display><type>article</type><title>Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Liu, Sai-Lan ; Lin, Huan-Xin ; Qiu, Fang ; Zhang, Wei-Jing ; Niu, Chun-Hao ; Wen, Wen ; Sun, Xiao-Qing ; Ye, Li-Ping ; Wu, Xian-Qiu ; Lin, Chu-Yong ; Song, Li-Bing ; Guo, Ling</creator><contributor>Sung, Shian-Ying</contributor><creatorcontrib>Liu, Sai-Lan ; Lin, Huan-Xin ; Qiu, Fang ; Zhang, Wei-Jing ; Niu, Chun-Hao ; Wen, Wen ; Sun, Xiao-Qing ; Ye, Li-Ping ; Wu, Xian-Qiu ; Lin, Chu-Yong ; Song, Li-Bing ; Guo, Ling ; Sung, Shian-Ying</creatorcontrib><description>Numerous studies have shown Kinesin family member 20A (KIF20A) may play a critical role in the development and progression of cancer. However, the clinical value of KIF20A in nasopharyngeal carcinoma (NPC) is unknown. Here, we investigated the expression pattern of KIF20A in NPC and its correlation with clinicopathological features of patients.
Real-time PCR and Western blotting were used to quantify KIF20A expression in NPC cell lines and clinical specimens compared with normal controls. KIF20A protein expression was also examined in archived paraffin embedded tumor samples from 105 patients with pathologically confirmed NPC by immunohistochemistry (IHC). Statistical analyses were applied to assess the associations between KIF20A expression and the clinicopathological features and survival outcomes. Effects on migration and invasion were assessed by wound healing and transwell invasion assays after KIF20A silencing.
KIF20A was significantly overexpressed at both the mRNA and protein levels in NPC cell lines and human tumor tissues. 45/105 (42.9%) of NPC specimens expressed high levels of KIF20A among the KIF20A detectable cases. Statistical analysis revealed that high KIF20A expression was significantly associated with gender (P = 0.046), clinical stage (P<0.001), T category (P = 0.022), N category (P<0.001), distant metastasis (P = 0.001) and vital status (P = 0.001). Moreover, Higher KIF20A expression patients had shorter overall survival (OS) and progression-free survival (PFS) (P = 0.001 and P = 0.001; log-rank test). In multivariate analysis, KIF20A was an independent prognostic factor for OS and PFS in the entire cohort (P = 0.033, P = 0.008). Knock down of KIF20A expression significantly suppressed NPC cell's migration and invasion.
KIF20A is overexpressed and may serve as an independent prognostic biomarker in NPC. Targeting KIF20A reduces migration and invasion of NPC cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0169280</identifier><identifier>PMID: 28081138</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Biology and life sciences ; Biomarkers ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Biotechnology ; Breast cancer ; Cancer ; Cell Line, Tumor ; Cell Movement ; Collaboration ; Disease Progression ; Female ; Follow-Up Studies ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Gynecology ; Humans ; Immunohistochemistry ; Immunotherapy ; Kinesin ; Kinesin - biosynthesis ; Laboratories ; Male ; Medical prognosis ; Medical research ; Medicine ; Medicine and Health Sciences ; Metastases ; Metastasis ; Middle Aged ; mRNA ; Multivariate analysis ; Nasopharyngeal cancer ; Nasopharyngeal carcinoma ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Nasopharyngeal Neoplasms - pathology ; Neoplasm Invasiveness ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Oncology ; Pancreatic cancer ; Paraffin ; Patients ; Prognosis ; Radiation therapy ; Research and Analysis Methods ; Statistical analysis ; Statistical methods ; Statistics ; Survival ; Throat cancer ; Tissues ; Tumor cell lines ; Vascular endothelial growth factor ; Western blotting ; Wound healing</subject><ispartof>PloS one, 2017-01, Vol.12 (1), p.e0169280-e0169280</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Liu et al 2017 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-a87f562cfda55ac158728bdadd127edb42467806d7ffcebd27e7dcc37b50248f3</citedby><cites>FETCH-LOGICAL-c725t-a87f562cfda55ac158728bdadd127edb42467806d7ffcebd27e7dcc37b50248f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5230771/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5230771/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28081138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sung, Shian-Ying</contributor><creatorcontrib>Liu, Sai-Lan</creatorcontrib><creatorcontrib>Lin, Huan-Xin</creatorcontrib><creatorcontrib>Qiu, Fang</creatorcontrib><creatorcontrib>Zhang, Wei-Jing</creatorcontrib><creatorcontrib>Niu, Chun-Hao</creatorcontrib><creatorcontrib>Wen, Wen</creatorcontrib><creatorcontrib>Sun, Xiao-Qing</creatorcontrib><creatorcontrib>Ye, Li-Ping</creatorcontrib><creatorcontrib>Wu, Xian-Qiu</creatorcontrib><creatorcontrib>Lin, Chu-Yong</creatorcontrib><creatorcontrib>Song, Li-Bing</creatorcontrib><creatorcontrib>Guo, Ling</creatorcontrib><title>Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Numerous studies have shown Kinesin family member 20A (KIF20A) may play a critical role in the development and progression of cancer. However, the clinical value of KIF20A in nasopharyngeal carcinoma (NPC) is unknown. Here, we investigated the expression pattern of KIF20A in NPC and its correlation with clinicopathological features of patients.
Real-time PCR and Western blotting were used to quantify KIF20A expression in NPC cell lines and clinical specimens compared with normal controls. KIF20A protein expression was also examined in archived paraffin embedded tumor samples from 105 patients with pathologically confirmed NPC by immunohistochemistry (IHC). Statistical analyses were applied to assess the associations between KIF20A expression and the clinicopathological features and survival outcomes. Effects on migration and invasion were assessed by wound healing and transwell invasion assays after KIF20A silencing.
KIF20A was significantly overexpressed at both the mRNA and protein levels in NPC cell lines and human tumor tissues. 45/105 (42.9%) of NPC specimens expressed high levels of KIF20A among the KIF20A detectable cases. Statistical analysis revealed that high KIF20A expression was significantly associated with gender (P = 0.046), clinical stage (P<0.001), T category (P = 0.022), N category (P<0.001), distant metastasis (P = 0.001) and vital status (P = 0.001). Moreover, Higher KIF20A expression patients had shorter overall survival (OS) and progression-free survival (PFS) (P = 0.001 and P = 0.001; log-rank test). In multivariate analysis, KIF20A was an independent prognostic factor for OS and PFS in the entire cohort (P = 0.033, P = 0.008). Knock down of KIF20A expression significantly suppressed NPC cell's migration and invasion.
KIF20A is overexpressed and may serve as an independent prognostic biomarker in NPC. Targeting KIF20A reduces migration and invasion of NPC cells.</description><subject>Adult</subject><subject>Aged</subject><subject>Biology and life sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Collaboration</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Gynecology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunotherapy</subject><subject>Kinesin</subject><subject>Kinesin - biosynthesis</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>mRNA</subject><subject>Multivariate analysis</subject><subject>Nasopharyngeal cancer</subject><subject>Nasopharyngeal carcinoma</subject><subject>Nasopharyngeal Neoplasms - genetics</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Nasopharyngeal Neoplasms - pathology</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Oncology</subject><subject>Pancreatic cancer</subject><subject>Paraffin</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Radiation therapy</subject><subject>Research and Analysis Methods</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Statistics</subject><subject>Survival</subject><subject>Throat cancer</subject><subject>Tissues</subject><subject>Tumor cell lines</subject><subject>Vascular endothelial growth factor</subject><subject>Western blotting</subject><subject>Wound healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBYQkJw0eKPJHa4QKoKYxWDTePj1nLsk9ZVYhc7Gdtv4k_ibu20ol1MuUh08ryvzzk-J8ueEzwmjJN3Sz8Ep9rxyjsYY1JWVOAH2T6pGB2VFLOHt773sicxLjEumCjLx9leQgUhTOxnf0_OIcDFKkCM1jvkG_TFOojWoUPV2fYSfYWuhoAonqCpDwFa1UNEf2y_QB9tBBUBnQY_3xooZ9Cp9-Eq6Hy0ESWvo6FTDn1T0a8WKly6OagWTZXTEN6jCTqDPvi4At3bc0CTVNblWpiSIbhAp6q34Pr4NHvUqDbCs837IPt5-OnH9Gh0fPJ5Np0cjzSnRT9SgjdFSXVjVFEoTQrBqaiNMoZQDqbOaV5ygUvDm0ZDbVKQG60ZrwtMc9Gwg-zlte-q9VFu-hwlEUVqW0V5lYjZNWG8WspVsF0qSnpl5VXAh7lUobe6BQmqrnOoqpKZMq8FUcBMzlhZVLjQgpXJ68PmtKHuwOhUaVDtjunuH2cXcu7PZUEZ5pwkgzcbg-B_DxB72dmooW2VAz-s8y4FwxUW_D4oyRNNRUJf_Yfe3YgNNVepVusan1LUa1M5yTmnFRNiTY3voNJjoLM6zW9jU3xH8HZHkJgeLvq5GmKUs-9n92dPfu2yr2-xizSE_SL6dujT5MZdML8GdZrLGKC5uQ-C5Xr9tt2Q6_WTm_VLshe37_JGtN039g9gMSyL</recordid><startdate>20170112</startdate><enddate>20170112</enddate><creator>Liu, Sai-Lan</creator><creator>Lin, Huan-Xin</creator><creator>Qiu, Fang</creator><creator>Zhang, Wei-Jing</creator><creator>Niu, Chun-Hao</creator><creator>Wen, Wen</creator><creator>Sun, Xiao-Qing</creator><creator>Ye, Li-Ping</creator><creator>Wu, Xian-Qiu</creator><creator>Lin, Chu-Yong</creator><creator>Song, Li-Bing</creator><creator>Guo, Ling</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170112</creationdate><title>Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients</title><author>Liu, Sai-Lan ; Lin, Huan-Xin ; Qiu, Fang ; Zhang, Wei-Jing ; Niu, Chun-Hao ; Wen, Wen ; Sun, Xiao-Qing ; Ye, Li-Ping ; Wu, Xian-Qiu ; Lin, Chu-Yong ; Song, Li-Bing ; Guo, Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-a87f562cfda55ac158728bdadd127edb42467806d7ffcebd27e7dcc37b50248f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biology and life sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - 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genetics</topic><topic>Nasopharyngeal Neoplasms - metabolism</topic><topic>Nasopharyngeal Neoplasms - pathology</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Oncology</topic><topic>Pancreatic cancer</topic><topic>Paraffin</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Radiation therapy</topic><topic>Research and Analysis Methods</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Statistics</topic><topic>Survival</topic><topic>Throat cancer</topic><topic>Tissues</topic><topic>Tumor cell lines</topic><topic>Vascular endothelial growth factor</topic><topic>Western blotting</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Sai-Lan</creatorcontrib><creatorcontrib>Lin, Huan-Xin</creatorcontrib><creatorcontrib>Qiu, Fang</creatorcontrib><creatorcontrib>Zhang, Wei-Jing</creatorcontrib><creatorcontrib>Niu, Chun-Hao</creatorcontrib><creatorcontrib>Wen, Wen</creatorcontrib><creatorcontrib>Sun, Xiao-Qing</creatorcontrib><creatorcontrib>Ye, Li-Ping</creatorcontrib><creatorcontrib>Wu, Xian-Qiu</creatorcontrib><creatorcontrib>Lin, Chu-Yong</creatorcontrib><creatorcontrib>Song, Li-Bing</creatorcontrib><creatorcontrib>Guo, Ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints in Context (Gale)</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Sai-Lan</au><au>Lin, Huan-Xin</au><au>Qiu, Fang</au><au>Zhang, Wei-Jing</au><au>Niu, Chun-Hao</au><au>Wen, Wen</au><au>Sun, Xiao-Qing</au><au>Ye, Li-Ping</au><au>Wu, Xian-Qiu</au><au>Lin, Chu-Yong</au><au>Song, Li-Bing</au><au>Guo, Ling</au><au>Sung, Shian-Ying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-01-12</date><risdate>2017</risdate><volume>12</volume><issue>1</issue><spage>e0169280</spage><epage>e0169280</epage><pages>e0169280-e0169280</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Numerous studies have shown Kinesin family member 20A (KIF20A) may play a critical role in the development and progression of cancer. However, the clinical value of KIF20A in nasopharyngeal carcinoma (NPC) is unknown. Here, we investigated the expression pattern of KIF20A in NPC and its correlation with clinicopathological features of patients.
Real-time PCR and Western blotting were used to quantify KIF20A expression in NPC cell lines and clinical specimens compared with normal controls. KIF20A protein expression was also examined in archived paraffin embedded tumor samples from 105 patients with pathologically confirmed NPC by immunohistochemistry (IHC). Statistical analyses were applied to assess the associations between KIF20A expression and the clinicopathological features and survival outcomes. Effects on migration and invasion were assessed by wound healing and transwell invasion assays after KIF20A silencing.
KIF20A was significantly overexpressed at both the mRNA and protein levels in NPC cell lines and human tumor tissues. 45/105 (42.9%) of NPC specimens expressed high levels of KIF20A among the KIF20A detectable cases. Statistical analysis revealed that high KIF20A expression was significantly associated with gender (P = 0.046), clinical stage (P<0.001), T category (P = 0.022), N category (P<0.001), distant metastasis (P = 0.001) and vital status (P = 0.001). Moreover, Higher KIF20A expression patients had shorter overall survival (OS) and progression-free survival (PFS) (P = 0.001 and P = 0.001; log-rank test). In multivariate analysis, KIF20A was an independent prognostic factor for OS and PFS in the entire cohort (P = 0.033, P = 0.008). Knock down of KIF20A expression significantly suppressed NPC cell's migration and invasion.
KIF20A is overexpressed and may serve as an independent prognostic biomarker in NPC. Targeting KIF20A reduces migration and invasion of NPC cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28081138</pmid><doi>10.1371/journal.pone.0169280</doi><tpages>e0169280</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-01, Vol.12 (1), p.e0169280-e0169280 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1858089279 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Biology and life sciences Biomarkers Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Biotechnology Breast cancer Cancer Cell Line, Tumor Cell Movement Collaboration Disease Progression Female Follow-Up Studies Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Gynecology Humans Immunohistochemistry Immunotherapy Kinesin Kinesin - biosynthesis Laboratories Male Medical prognosis Medical research Medicine Medicine and Health Sciences Metastases Metastasis Middle Aged mRNA Multivariate analysis Nasopharyngeal cancer Nasopharyngeal carcinoma Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Nasopharyngeal Neoplasms - pathology Neoplasm Invasiveness Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Oncology Pancreatic cancer Paraffin Patients Prognosis Radiation therapy Research and Analysis Methods Statistical analysis Statistical methods Statistics Survival Throat cancer Tissues Tumor cell lines Vascular endothelial growth factor Western blotting Wound healing |
| title | Overexpression of Kinesin Family Member 20A Correlates with Disease Progression and Poor Prognosis in Human Nasopharyngeal Cancer: A Retrospective Analysis of 105 Patients |
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