Upregulation of Far Upstream Element-Binding Protein 1 (FUBP1) Promotes Tumor Proliferation and Tumorigenesis of Clear Cell Renal Cell Carcinoma
The far upstream element (FUSE)-binding protein 1 (FUBP1) is a transactivator of human c-myc proto-oncogene transcription, with important roles in carcinogenesis. However, the expression pattern and potential biological function of FUBP1 in clear cell renal cell carcinoma (ccRCC) is yet to be establ...
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| Veröffentlicht in: | PloS one 2017-01, Vol.12 (1), p.e0169852 |
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| creator | Duan, Junyao Bao, Xu Ma, Xin Zhang, Yu Ni, Dong Wang, Hanfeng Zhang, Fan Du, Qingshan Fan, Yang Chen, Jianwen Wu, Shengpan Li, Xintao Gao, Yu Zhang, Xu |
| description | The far upstream element (FUSE)-binding protein 1 (FUBP1) is a transactivator of human c-myc proto-oncogene transcription, with important roles in carcinogenesis. However, the expression pattern and potential biological function of FUBP1 in clear cell renal cell carcinoma (ccRCC) is yet to be established.
FUBP1 expression was detected in ccRCC tissues and cell lines by real-time RT-PCR, Western blot analysis, and immunohistochemistry. The correlations of FUBP1 mRNA expression levels with clinicopathological factors were evaluated. The biological function of FUBP1 during tumor cell proliferation was studied by MTS, colony formation, and soft-agar colony formation. The effects of FUBP1 on cell cycle distribution and apoptosis were analyzed by flow cytometry. Western blot analysis was used to identify the potential mechanism of FUBP1 regulating cell cycle and apoptosis.
The levels of FUBP1 mRNA and protein expression were upregulated in human ccRCC tissues compared with adjacent noncancerous tissues. High levels of FUBP1 mRNA expression were associated with higher tumor stage and tumor size. FUBP1 knockdown inhibited cell proliferation and induced cell cycle arrest and apoptosis. Meanwhile, the expression levels of c-myc and p21 mRNA were correlated with that of FUBP1 mRNA.
FUBP1 acts as a potential oncogene in ccRCC and may be considered as a novel biomarker or an attractive treatment target of ccRCC. |
| doi_str_mv | 10.1371/journal.pone.0169852 |
| format | Article |
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FUBP1 expression was detected in ccRCC tissues and cell lines by real-time RT-PCR, Western blot analysis, and immunohistochemistry. The correlations of FUBP1 mRNA expression levels with clinicopathological factors were evaluated. The biological function of FUBP1 during tumor cell proliferation was studied by MTS, colony formation, and soft-agar colony formation. The effects of FUBP1 on cell cycle distribution and apoptosis were analyzed by flow cytometry. Western blot analysis was used to identify the potential mechanism of FUBP1 regulating cell cycle and apoptosis.
The levels of FUBP1 mRNA and protein expression were upregulated in human ccRCC tissues compared with adjacent noncancerous tissues. High levels of FUBP1 mRNA expression were associated with higher tumor stage and tumor size. FUBP1 knockdown inhibited cell proliferation and induced cell cycle arrest and apoptosis. Meanwhile, the expression levels of c-myc and p21 mRNA were correlated with that of FUBP1 mRNA.
FUBP1 acts as a potential oncogene in ccRCC and may be considered as a novel biomarker or an attractive treatment target of ccRCC.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0169852</identifier><identifier>PMID: 28076379</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Agar ; Aged ; Aged, 80 and over ; Analysis ; Apoptosis ; Bioindicators ; Biology and Life Sciences ; Biomarkers ; Biomarkers, Tumor - genetics ; c-Myc protein ; Carcinogenesis ; Carcinogenesis - genetics ; Carcinogens ; Carcinoma, Renal Cell - genetics ; Carcinoma, Renal Cell - pathology ; Care and treatment ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - genetics ; Cell size ; Cell Transformation, Neoplastic - genetics ; Clear cell-type renal cell carcinoma ; Colonies ; Correlation analysis ; Cyclin-dependent kinases ; Cytometry ; Development and progression ; DNA Helicases - genetics ; DNA-Binding Proteins - genetics ; Far upstream element-binding protein ; Female ; Flow cytometry ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Hospitals ; Humans ; Immunohistochemistry ; Kidney cancer ; Kidney diseases ; Kidney Neoplasms - genetics ; Kidney Neoplasms - pathology ; Kinases ; Laboratories ; Liver cancer ; Male ; Medical schools ; Medicine and Health Sciences ; Middle Aged ; Myc protein ; Polymerase chain reaction ; Protein binding ; Proteins ; Renal cell carcinoma ; Research and Analysis Methods ; Tissues ; Transcription ; Transcriptional Activation ; Tumorigenesis ; Tumors ; Up-Regulation - genetics ; Upstream ; Urology</subject><ispartof>PloS one, 2017-01, Vol.12 (1), p.e0169852</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Duan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Duan et al 2017 Duan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c626t-b5e81abd762c0c6ea78063a829deee0135097fcaaac790f5c583d39fc038e6d33</citedby><cites>FETCH-LOGICAL-c626t-b5e81abd762c0c6ea78063a829deee0135097fcaaac790f5c583d39fc038e6d33</cites><orcidid>0000-0002-0608-6509</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226774/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226774/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28076379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Junyao</creatorcontrib><creatorcontrib>Bao, Xu</creatorcontrib><creatorcontrib>Ma, Xin</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Ni, Dong</creatorcontrib><creatorcontrib>Wang, Hanfeng</creatorcontrib><creatorcontrib>Zhang, Fan</creatorcontrib><creatorcontrib>Du, Qingshan</creatorcontrib><creatorcontrib>Fan, Yang</creatorcontrib><creatorcontrib>Chen, Jianwen</creatorcontrib><creatorcontrib>Wu, Shengpan</creatorcontrib><creatorcontrib>Li, Xintao</creatorcontrib><creatorcontrib>Gao, Yu</creatorcontrib><creatorcontrib>Zhang, Xu</creatorcontrib><title>Upregulation of Far Upstream Element-Binding Protein 1 (FUBP1) Promotes Tumor Proliferation and Tumorigenesis of Clear Cell Renal Cell Carcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The far upstream element (FUSE)-binding protein 1 (FUBP1) is a transactivator of human c-myc proto-oncogene transcription, with important roles in carcinogenesis. However, the expression pattern and potential biological function of FUBP1 in clear cell renal cell carcinoma (ccRCC) is yet to be established.
FUBP1 expression was detected in ccRCC tissues and cell lines by real-time RT-PCR, Western blot analysis, and immunohistochemistry. The correlations of FUBP1 mRNA expression levels with clinicopathological factors were evaluated. The biological function of FUBP1 during tumor cell proliferation was studied by MTS, colony formation, and soft-agar colony formation. The effects of FUBP1 on cell cycle distribution and apoptosis were analyzed by flow cytometry. Western blot analysis was used to identify the potential mechanism of FUBP1 regulating cell cycle and apoptosis.
The levels of FUBP1 mRNA and protein expression were upregulated in human ccRCC tissues compared with adjacent noncancerous tissues. High levels of FUBP1 mRNA expression were associated with higher tumor stage and tumor size. FUBP1 knockdown inhibited cell proliferation and induced cell cycle arrest and apoptosis. Meanwhile, the expression levels of c-myc and p21 mRNA were correlated with that of FUBP1 mRNA.
FUBP1 acts as a potential oncogene in ccRCC and may be considered as a novel biomarker or an attractive treatment target of ccRCC.</description><subject>Adult</subject><subject>Agar</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Analysis</subject><subject>Apoptosis</subject><subject>Bioindicators</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>c-Myc protein</subject><subject>Carcinogenesis</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogens</subject><subject>Carcinoma, Renal Cell - genetics</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Cell size</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>Clear cell-type renal cell carcinoma</subject><subject>Colonies</subject><subject>Correlation analysis</subject><subject>Cyclin-dependent kinases</subject><subject>Cytometry</subject><subject>Development and progression</subject><subject>DNA Helicases - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Far upstream element-binding protein</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney cancer</subject><subject>Kidney diseases</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidney Neoplasms - pathology</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Male</subject><subject>Medical schools</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Myc protein</subject><subject>Polymerase chain reaction</subject><subject>Protein binding</subject><subject>Proteins</subject><subject>Renal cell carcinoma</subject><subject>Research and Analysis Methods</subject><subject>Tissues</subject><subject>Transcription</subject><subject>Transcriptional Activation</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Up-Regulation - 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genetics</topic><topic>c-Myc protein</topic><topic>Carcinogenesis</topic><topic>Carcinogenesis - genetics</topic><topic>Carcinogens</topic><topic>Carcinoma, Renal Cell - genetics</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Cell size</topic><topic>Cell Transformation, Neoplastic - genetics</topic><topic>Clear cell-type renal cell carcinoma</topic><topic>Colonies</topic><topic>Correlation analysis</topic><topic>Cyclin-dependent kinases</topic><topic>Cytometry</topic><topic>Development and progression</topic><topic>DNA Helicases - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Far upstream element-binding protein</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kidney cancer</topic><topic>Kidney diseases</topic><topic>Kidney Neoplasms - 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However, the expression pattern and potential biological function of FUBP1 in clear cell renal cell carcinoma (ccRCC) is yet to be established.
FUBP1 expression was detected in ccRCC tissues and cell lines by real-time RT-PCR, Western blot analysis, and immunohistochemistry. The correlations of FUBP1 mRNA expression levels with clinicopathological factors were evaluated. The biological function of FUBP1 during tumor cell proliferation was studied by MTS, colony formation, and soft-agar colony formation. The effects of FUBP1 on cell cycle distribution and apoptosis were analyzed by flow cytometry. Western blot analysis was used to identify the potential mechanism of FUBP1 regulating cell cycle and apoptosis.
The levels of FUBP1 mRNA and protein expression were upregulated in human ccRCC tissues compared with adjacent noncancerous tissues. High levels of FUBP1 mRNA expression were associated with higher tumor stage and tumor size. FUBP1 knockdown inhibited cell proliferation and induced cell cycle arrest and apoptosis. Meanwhile, the expression levels of c-myc and p21 mRNA were correlated with that of FUBP1 mRNA.
FUBP1 acts as a potential oncogene in ccRCC and may be considered as a novel biomarker or an attractive treatment target of ccRCC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28076379</pmid><doi>10.1371/journal.pone.0169852</doi><orcidid>https://orcid.org/0000-0002-0608-6509</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-01, Vol.12 (1), p.e0169852 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1857740197 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adult Agar Aged Aged, 80 and over Analysis Apoptosis Bioindicators Biology and Life Sciences Biomarkers Biomarkers, Tumor - genetics c-Myc protein Carcinogenesis Carcinogenesis - genetics Carcinogens Carcinoma, Renal Cell - genetics Carcinoma, Renal Cell - pathology Care and treatment Cell cycle Cell growth Cell Line, Tumor Cell proliferation Cell Proliferation - genetics Cell size Cell Transformation, Neoplastic - genetics Clear cell-type renal cell carcinoma Colonies Correlation analysis Cyclin-dependent kinases Cytometry Development and progression DNA Helicases - genetics DNA-Binding Proteins - genetics Far upstream element-binding protein Female Flow cytometry Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Hospitals Humans Immunohistochemistry Kidney cancer Kidney diseases Kidney Neoplasms - genetics Kidney Neoplasms - pathology Kinases Laboratories Liver cancer Male Medical schools Medicine and Health Sciences Middle Aged Myc protein Polymerase chain reaction Protein binding Proteins Renal cell carcinoma Research and Analysis Methods Tissues Transcription Transcriptional Activation Tumorigenesis Tumors Up-Regulation - genetics Upstream Urology |
| title | Upregulation of Far Upstream Element-Binding Protein 1 (FUBP1) Promotes Tumor Proliferation and Tumorigenesis of Clear Cell Renal Cell Carcinoma |
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