Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution
A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae fa...
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| creator | Pozo, Francisco Juste, Javier Vázquez-Morón, Sonia Aznar-López, Carolina Ibáñez, Carlos Garin, Inazio Aihartza, Joxerra Casas, Inmaculada Tenorio, Antonio Echevarría, Juan Emilio |
| description | A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae family. By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. This supports the hypothesis of a species-specific parallel evolution process between the potentially new betaherpesviruses and their bat hosts. Interestingly, two of the betaherpesviruses' sequences detected in rhinolophid bats clustered together apart from the rest, closely related to viruses that belong to the Roseolovirus genus. This suggests a putative third roseolo lineage. On the contrary, no phylogenetic structure was detected among several potentially novel bat-hosted gammaherpesviruses found in the study. Remarkably, all of the possible novel bat herpesviruses described in this study are linked to a unique bat species. |
| doi_str_mv | 10.1371/journal.pone.0169153 |
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By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. This supports the hypothesis of a species-specific parallel evolution process between the potentially new betaherpesviruses and their bat hosts. Interestingly, two of the betaherpesviruses' sequences detected in rhinolophid bats clustered together apart from the rest, closely related to viruses that belong to the Roseolovirus genus. This suggests a putative third roseolo lineage. On the contrary, no phylogenetic structure was detected among several potentially novel bat-hosted gammaherpesviruses found in the study. Remarkably, all of the possible novel bat herpesviruses described in this study are linked to a unique bat species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0169153</identifier><identifier>PMID: 28036408</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Base Sequence ; Bats ; Bats (Animals) ; Betaherpesvirinae ; Betaherpesvirinae - classification ; Betaherpesvirinae - genetics ; Betaherpesvirinae - growth & development ; Betaherpesvirinae - isolation & purification ; Biological Evolution ; Biology ; Biology and Life Sciences ; Chiroptera ; Chiroptera - virology ; Computer and Information Sciences ; Convergent evolution ; Cytomegalovirus ; DNA, Viral - genetics ; Evolution ; Gammaherpesvirinae ; Gammaherpesvirinae - classification ; Gammaherpesvirinae - genetics ; Gammaherpesvirinae - isolation & purification ; Genetic aspects ; Genetic Variation - genetics ; Herpesviridae ; Herpesviruses ; Identification and classification ; Mammals ; Medicine and Health Sciences ; Miniopteridae ; Molossidae ; Myotis lucifugus ; Phylogenetics ; Phylogeny ; Polymerase Chain Reaction ; Portugal ; Pteropodidae ; Research and Analysis Methods ; Rhinolophidae ; Roseolovirus ; Roseolovirus - classification ; Roseolovirus - genetics ; Sequence Alignment ; Sequence Analysis, DNA ; Spain ; Species ; Taxonomy ; Vespertilionidae ; Virology ; Viruses ; Zoology</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0169153-e0169153</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Pozo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Pozo et al 2016 Pozo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-db909e8db7f87f5c3726f6e5c53a6d9115e2a8921ec1fa875fdd86907f15de653</citedby><cites>FETCH-LOGICAL-c725t-db909e8db7f87f5c3726f6e5c53a6d9115e2a8921ec1fa875fdd86907f15de653</cites><orcidid>0000-0003-1828-733X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5201282/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5201282/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28036408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Forrester, Naomi</contributor><creatorcontrib>Pozo, Francisco</creatorcontrib><creatorcontrib>Juste, Javier</creatorcontrib><creatorcontrib>Vázquez-Morón, Sonia</creatorcontrib><creatorcontrib>Aznar-López, Carolina</creatorcontrib><creatorcontrib>Ibáñez, Carlos</creatorcontrib><creatorcontrib>Garin, Inazio</creatorcontrib><creatorcontrib>Aihartza, Joxerra</creatorcontrib><creatorcontrib>Casas, Inmaculada</creatorcontrib><creatorcontrib>Tenorio, Antonio</creatorcontrib><creatorcontrib>Echevarría, Juan Emilio</creatorcontrib><title>Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae family. By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. 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isolation & purification</subject><subject>Genetic aspects</subject><subject>Genetic Variation - genetics</subject><subject>Herpesviridae</subject><subject>Herpesviruses</subject><subject>Identification and classification</subject><subject>Mammals</subject><subject>Medicine and Health Sciences</subject><subject>Miniopteridae</subject><subject>Molossidae</subject><subject>Myotis lucifugus</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Portugal</subject><subject>Pteropodidae</subject><subject>Research and Analysis Methods</subject><subject>Rhinolophidae</subject><subject>Roseolovirus</subject><subject>Roseolovirus - classification</subject><subject>Roseolovirus - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Spain</subject><subject>Species</subject><subject>Taxonomy</subject><subject>Vespertilionidae</subject><subject>Virology</subject><subject>Viruses</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0WLH8dcN0jYNqDQxNAa3lhMft67SuLOTCv49bptNDdrF5Atb9vO-xz4-J8teYzTFhONPS9-HVjfTtW9hijCTmJIn2TGWpJiwApGnB-uj7EWMS4QoEYw9z44KgQgrkTjObmYG2s5ZV-vO-Tb3Nv_uN9DkZ9DpBYQ1xI0LfYSYuzafVRCcbvMz3cX8Gjagm5j_0EE3TZJcbHzTb11eZs9sOoFXw3yS_fpycXP-bXJ59XV2fno5qXlBu4mpJJIgTMWt4JbWhBfMMqA1JZoZiTGFQgtZYKix1YJTa4xgEnGLqQFGyUn2du-7bnxUQ0KiwoKWBEnGRCJme8J4vVTr4FY6_FVeO7Xb8GGudOhc3YCihakqqFhJES8xtiJF4ZUmtuKSG7GN9nmI1lcrMHXKW3r4yHR80rqFmvtNcka4EEUy-DAYBH_bQ-zUysUamka34PvdvWUpEZLlY9CSYc6FTOi7_9CHEzFQc53e6lrr0xXrrak6LTlNHNuFnT5ApWFg5epUaNal_ZHg40iQmA7-dHPdx6hmP68fz179HrPvD9hFqrNuEYfqimOw3IN18DEGsPf_gZHa9sldNtS2T9TQJ0n25vAv70V3jUH-AdkKDPA</recordid><startdate>20161230</startdate><enddate>20161230</enddate><creator>Pozo, Francisco</creator><creator>Juste, Javier</creator><creator>Vázquez-Morón, Sonia</creator><creator>Aznar-López, Carolina</creator><creator>Ibáñez, Carlos</creator><creator>Garin, Inazio</creator><creator>Aihartza, Joxerra</creator><creator>Casas, Inmaculada</creator><creator>Tenorio, Antonio</creator><creator>Echevarría, Juan Emilio</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1828-733X</orcidid></search><sort><creationdate>20161230</creationdate><title>Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution</title><author>Pozo, Francisco ; Juste, Javier ; Vázquez-Morón, Sonia ; Aznar-López, Carolina ; Ibáñez, Carlos ; Garin, Inazio ; Aihartza, Joxerra ; Casas, Inmaculada ; Tenorio, Antonio ; Echevarría, Juan Emilio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-db909e8db7f87f5c3726f6e5c53a6d9115e2a8921ec1fa875fdd86907f15de653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Bats</topic><topic>Bats (Animals)</topic><topic>Betaherpesvirinae</topic><topic>Betaherpesvirinae - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pozo, Francisco</au><au>Juste, Javier</au><au>Vázquez-Morón, Sonia</au><au>Aznar-López, Carolina</au><au>Ibáñez, Carlos</au><au>Garin, Inazio</au><au>Aihartza, Joxerra</au><au>Casas, Inmaculada</au><au>Tenorio, Antonio</au><au>Echevarría, Juan Emilio</au><au>Forrester, Naomi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-30</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0169153</spage><epage>e0169153</epage><pages>e0169153-e0169153</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae family. By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. This supports the hypothesis of a species-specific parallel evolution process between the potentially new betaherpesviruses and their bat hosts. Interestingly, two of the betaherpesviruses' sequences detected in rhinolophid bats clustered together apart from the rest, closely related to viruses that belong to the Roseolovirus genus. This suggests a putative third roseolo lineage. On the contrary, no phylogenetic structure was detected among several potentially novel bat-hosted gammaherpesviruses found in the study. Remarkably, all of the possible novel bat herpesviruses described in this study are linked to a unique bat species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28036408</pmid><doi>10.1371/journal.pone.0169153</doi><tpages>e0169153</tpages><orcidid>https://orcid.org/0000-0003-1828-733X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-12, Vol.11 (12), p.e0169153-e0169153 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1854309668 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Bats Bats (Animals) Betaherpesvirinae Betaherpesvirinae - classification Betaherpesvirinae - genetics Betaherpesvirinae - growth & development Betaherpesvirinae - isolation & purification Biological Evolution Biology Biology and Life Sciences Chiroptera Chiroptera - virology Computer and Information Sciences Convergent evolution Cytomegalovirus DNA, Viral - genetics Evolution Gammaherpesvirinae Gammaherpesvirinae - classification Gammaherpesvirinae - genetics Gammaherpesvirinae - isolation & purification Genetic aspects Genetic Variation - genetics Herpesviridae Herpesviruses Identification and classification Mammals Medicine and Health Sciences Miniopteridae Molossidae Myotis lucifugus Phylogenetics Phylogeny Polymerase Chain Reaction Portugal Pteropodidae Research and Analysis Methods Rhinolophidae Roseolovirus Roseolovirus - classification Roseolovirus - genetics Sequence Alignment Sequence Analysis, DNA Spain Species Taxonomy Vespertilionidae Virology Viruses Zoology |
| title | Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution |
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