Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non-Specificity, and Inhibition

The single-strand-specific S1 nuclease from Aspergillus oryzae is an archetypal enzyme of the S1-P1 family of nucleases with a widespread use for biochemical analyses of nucleic acids. We present the first X-ray structure of this nuclease along with a thorough analysis of the reaction and inhibition...

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Veröffentlicht in:	PloS one 2016-12, Vol.11 (12), p.e0168832-e0168832
Hauptverfasser:	Kovaľ, Tomáš, Østergaard, Lars H, Lehmbeck, Jan, Nørgaard, Allan, Lipovová, Petra, Dušková, Jarmila, Skálová, Tereza, Trundová, Mária, Kolenko, Petr, Fejfarová, Karla, Stránský, Jan, Švecová, Leona, Hašek, Jindřich, Dohnálek, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Amino Acids - metabolism Analysis Aspergillus Aspergillus oryzae Aspergillus oryzae - enzymology Aspergillus oryzae - metabolism Binding sites Binding Sites - physiology Biochemistry Biology and life sciences Biotechnology Catalysis Catalytic Domain - physiology Coordination compounds Crystallography Deoxyribonucleic acid DNA DNA binding proteins Enzymes Fungal Proteins - metabolism Kinetics Laboratories Ligands Mutants Nuclease Nucleases Nucleic acids Nucleosides Nucleotides Phosphates Physical Sciences Preferences Properties (attributes) Proteins Sequence Alignment Single-Strand Specific DNA and RNA Endonucleases - metabolism Substrate inhibition Substrate Specificity Sugar
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